161,273 results

1. pJW1311

   Plasmid

   #61254

   Purpose

   Template for cloning sgRNA(F+E) to generate new PU6::sgRNAs by PCR-fusion

   Depositor

   Jordan Ward

   Article

   Ward et al Genetics. 2014 Dec 9. pii: genetics.114.

   Insert

   sgRNA(F+E) targeting Y61A9L9.1
   

   Use

   CRISPR; Cloning template

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. AiP13563 - pAAV-AiE0170h_3xC2-minBG-SYFP2-WPRE3-BGHpA (Alias: CN3563)

   Plasmid

   #224029

   Purpose

   AiE0170h_3xC2 is an enhancer sequence, designed to drive AAV-mediated transgene expression in cortical Sst+ neurons

   Depositor

   The Allen Institute for Brain Science, Jonathan Ting

   Article

   Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

   Insert

   SYFP2
   

   Use

   AAV

   Tags

   Expression

   Mutation

   NA

   Promoter

   minBG

   Available since

   Oct. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLZ12Km2-P23R:TA:Xen5

   Plasmid

   #88902

   Purpose

   Toxin-antitoxin stabilized bioluminescent reporter plasmid for Streptococcus expression

   Depositor

   Thomas Proft

   Article

   Loh et al Appl Microbiol Biotechnol. 2013 Nov;97(2

   Insert

   toxin-antitoxin + LuxABCDE
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 5, 2017

   Availability

   Academic Institutions and Nonprofits only

4. pPYL-ABI

   Plasmid

   #233650

   Purpose

   Cellular colocalization assay (PYL/ABI) for the CIP OP (opabactin) or the CIP ABA (abscisic acid)

   Depositor

   Richard Wombacher

   Article

   Poschko et al ACS Chem Biol. 2025 Jan 27. doi: 10.1021

   Insert

   TOMM20-mCherry-PYL-P2A-eGFP-ABI
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. hMLKL NB(1-182)-2xFv-Venus

   Plasmid

   #106077

   Purpose

   expression in mammalian cells

   Depositor

   Douglas Green

   Article

   Quarato et al Mol Cell. 2016 Feb 18;61(4):589-601. doi

   Insert

   human MLKL NB(1-182)-2xFv-Venus (MLKL Human)
   

   Use

   Retroviral; Tet-on retrovirus

   Tags

   Venus

   Expression

   Mammalian

   Mutation

   amino acids 1-182

   Promoter

   Dox-inducible

   Available since

   March 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1-BCR-ABL-p210-FLAG

   Plasmid

   #205619

   Purpose

   Expression of BCR-ABL oncogene in mammalian cells

   Depositor

   Pavel Krejčí

   Article

   Gregor et al Cell Mol Life Sci. 2020 Oct;77(19):3885-

   Insert

   BCR-ABL oncogene, p210 isoform b3a2
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAAV-Ef1a-DO_DIO-TdTomato_EGFP-WPRE-pA

   Plasmid

   #37120

   Purpose

   DO/DIO Cre-Switch. Expresses tdTomato in Cre negative cells, expresses EGFP in Cre positive cells

   Depositor

   Bernardo Sabatini

   Article

   Saunders et al Front Neural Circuits. 2012 Jul 27;6:47.

   Insert

   TdTomato-EGFP
   

   Use

   AAV and Cre/Lox; Cre-switch

   Tags

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   July 27, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAcCas12nHS_ABE_D3_empty

   Plasmid

   #203809

   Purpose

   Plasmid containing a CMV-driven NLS-dAcCas12n(D240)-ABE-Design3-NLS cassette and a U6-driven sgRNA_V6 cassette(containing BsaI sites for spacer insertion).

   Depositor

   Quanjiang Ji

   Article

   Chen et al Mol Cell. 2023 Jun 27:S1097-2765(23)0046

   Insert

   ABE_D3
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   CMV

   Available since

   Aug. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pLenti_hU6-peg(ATP1A1-T804N)_mU6-BsmBI_Puro

   Plasmid

   #234070

   Purpose

   For lentiviral expression of user-specified pegRNA in tandem with epegRNA (ATP1A1-T804N) for ouabain co-selection

   Depositor

   Greg Findlay

   Article

   Herger et al Cell Genom. 2025 Mar 18:100814. doi: 10.

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   March 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-Syn1-tTA

    Plasmid

    #104109

    Purpose

    AAV-mediated expression of tTA under the Syn1 promoter.

    Depositor

    Takeshi Imai

    Article

    Sakaguchi et al eLife. 2018 Nov 20;7. pii: 40350. doi: 1

    Insert

    tTA2
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    hSyn1

    Available since

    June 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEGFP-hsRPA70 (MSW#473)

    Plasmid

    #208067

    Purpose

    Expression of GFP-tagged hsRPA1 in human cells

    Depositor

    Marc Wold

    Article

    Haring et al J Biol Chem. 2008 Jul 4;283(27):19095-11

    Insert

    RPA1 (RPA1 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA3β-FLAG-CBP-HA

    Plasmid

    #32908

    Depositor

    Tso-Pang Yao

    Article

    Zhao et al Mol Cell Biol. 2005 Oct;25(19):8456-64.

    Insert

    CBP (Crebbp Mouse)
    

    Use

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 18, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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13. pFUW-TetO-ETV6

    Plasmid

    #131594

    Purpose

    doxycycline-inducible expression of human ETV6 in mammalian cells

    Depositor

    Kateri Moore, Filipe Pereira

    Article

    Pereira et al Cell Stem Cell. 2013 Aug 1;13(2):205-18.

    Insert

    ETV6 (ETV6 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE-mCMV

    Available since

    Dec. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. GFP-(SUMO)3

    Plasmid

    #127093

    Purpose

    Bacterial expression plasmid for eGFP-(SUMO)3 client to assess SUMO-SIM scaffolds.

    Depositor

    Michael Rosen

    Article

    Banani et al Cell. 2016 Jul 28;166(3):651-663. doi: 1

    Insert

    EGFP - 3 repeats of human SUMO3 each separated by (GGS)4 (SUMO3 Human)
    

    Use

    Tags

    EGFP

    Expression

    Bacterial

    Mutation

    Promoter

    unknown

    Available since

    Sept. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. H2B-GFP-Aurora B WT

    Plasmid

    #184042

    Purpose

    Expresses H2B-GFP fused to Aurora B WT

    Depositor

    Lienhard Schmitz

    Article

    Pfisterer et al Open Biol. 2024 May;14(5):230460. doi: 1

    Insert

    AURKB (AURKB Human)
    

    Use

    Tags

    EGFP and H2B

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAAV-TPH2-Cre

    Plasmid

    #189616

    Purpose

    Expresses Cre recombinase under the control of the tryptophan hydroxylase promoter

    Depositor

    David Clapham

    Article

    Sheu et al Cell. 2022 Sep 1;185(18):3390-3407.e18.

    Insert

    Cre Recombinase
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Tph2

    Available since

    March 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRU41

    Plasmid

    #167663

    Purpose

    ENTRY vector for gRNA1 cloning under pAtU6 promoter

    Depositor

    Niko Geldner

    Article

    Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

    Insert

    pAtU6
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pALPS vpu

    Plasmid

    #101330

    Purpose

    Encodes codon optimized NL4-3 vpu

    Depositor

    Jeremy Luban

    Article

    McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

    Insert

    NL4-3 vpu
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAW-Nb127D01-GFP

    Plasmid

    #171571

    Purpose

    Expresses GFP-tagged anti-CXCR2 (human) recombinant llama nanobody (Nb127D01-GFP) in fly cells

    Depositor

    Norbert Perrimon, Hidde Ploegh

    Article

    Xu et al Elife. 2022 Jan 25;11. pii: 74326. doi:

    Insert

    Nb127D01-GFP (CXCR2 Human)
    

    Use

    Tags

    GFP

    Expression

    Insect

    Mutation

    Promoter

    fly actin5C promoter

    Available since

    June 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAGD335

    Plasmid

    #219499

    Purpose

    pAGD248 derivative with the retron Eco1 msr, sfGFP flanked by 2 BsaI sites , and the rest of the retron recombineering operon (Eco1 RT, CspRect, MutL-E32K) downstream of the pBad promoter

    Depositor

    Seth Shipman

    Article

    Gonzalez-Delgado et al bioRxiv [Preprint]. 2023 Jul 17:2023.07.

    Insert

    Eco1 msr, sfGFP, Eco1 RT, CspRecT, MutLE32K
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pBAD

    Available since

    June 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. WT Smarca4-sfGFP

    Plasmid

    #107056

    Purpose

    Expression of wild-type Smarca4 (Brg1) with C-terminal super-folder GFP fusion.

    Depositor

    Courtney Hodges, Jerry Crabtree

    Article

    Hodges et al Nat Struct Mol Biol. 2018 Jan;25(1):61-7

    Insert

    SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4 (Smarca4 Mouse)
    

    Use

    Lentiviral

    Tags

    Super-folder GFP (sfGFP)

    Expression

    Mammalian

    Mutation

    wild-type

    Promoter

    CAG

    Available since

    April 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pLR16-Phes*

    Plasmid

    #98783

    Purpose

    Suicide vector for allelic exchange in Listeria utilizing an engineered counterselection marker for Listeria monocytogenes based on a mutated phenylalanyl-tRNA synthetase gene (pheS*).

    Depositor

    Anat Herskovits

    Article

    Argov et al Appl Environ Microbiol. 2017 Mar 2;83(6)

    Insert

    pheS
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    A309G

    Promoter

    Available since

    Jan. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pGWB506

    Plasmid

    #74848

    Purpose

    Gateway cloning compatible binary vector for N-terminal fusion with sGFP (CaMV35S promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    CaMV35S

    Available since

    Aug. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pmCherry-C1 R62D actin-3XNLS P2A mCherry

    Plasmid

    #58477

    Purpose

    Expresses nuclear-targeted non-polymerizing R62D mutant of human actin, with an mCherry expression reporter after a P2A protease cleavage site, on a CMV promoter

    Depositor

    Dyche Mullins

    Article

    Belin et al Elife. 2015 Aug 19;4. doi: 10.7554/eLife

    Inserts

    beta actin (ACTB Human)
    3XNLS
    P2A cleavage site
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Changed Arginine 62 to Aspartic Acid

    Promoter

    CMV

    Available since

    Sept. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCMV_eA3A-NG-nCas9

    Plasmid

    #163536

    Purpose

    Mammalian CG-to-GC base editing

    Depositor

    David Liu

    Article

    Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

    Insert

    eA3A-NG-nCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    nCas9 (D10A)NG (L111R, D1135V, G1218R, E1219F, A1…

    Promoter

    Available since

    Dec. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. AAV-mOXT-hM3Dq-mCherry-WPRE

    Plasmid

    #70717

    Purpose

    Expresses DREADDs Gq receptor under oxytocin promoter control

    Depositor

    Daniel Geschwind

    Article

    Penagarikano et al Sci Transl Med. 2015 Jan 21;7(271):271ra

    Insert

    hM3Dq-mCherry
    

    Use

    AAV

    Tags

    mCherry

    Expression

    Mutation

    Promoter

    mouse oxytocin

    Available since

    Jan. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pcDNA5-FRT/TO-3*Flag-APEX2 N-term RNPS1

    Plasmid

    #187574

    Purpose

    Express FLAG epitope and APEX2-tagged RNPS1 fusion protein in mammalian cells

    Depositor

    Benjamin Blencowe

    Article

    Barutcu et al Mol Cell. 2022 Mar 3;82(5):1035-1052.e9.

    Insert

    RNPS1 (RNPS1 Human)
    

    Use

    Tags

    APEX2-FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pPAGFP-VSVG

    Plasmid

    #11915

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Presley et al Nature. 1997 Sep 4. 389(6646):81-5.

    Insert

    VSVG
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    (L64F/T65S/V163A/T203H). The PAGFP also contains …

    Promoter

    Available since

    July 3, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pAAV-hSyn-fDIO-hM4D(Gi)-mCherry-WPREpA

    Plasmid

    #154867

    Purpose

    Flp-dependent hM4D(Gi) DREADD-mCherry

    Depositor

    Ulrik Gether

    Article

    Runegaard et al eNeuro. 2018 Jun 18;5(3). pii: eN-NWR-03

    Has Service

    AAV Retrograde and AAV8

    Insert

    hM4D(Gi)-mCherry
    

    Use

    AAV; Flp-frt

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Human Synapsin-1

    Available since

    Aug. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pGEM4Z-Fluc

    Plasmid

    #183474

    Purpose

    This plasmid can be used to prepare Fluc mRNA by In Vitro Transcription

    Depositor

    Chantal Pichon

    Article

    Perche et al Mol Ther Nucleic Acids. 2019 Sep 6;17:76

    Insert

    Firefly luciferase
    

    Use

    Luciferase

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. AiP12109 - pAAV-AiE0410m-minBglobin-SYFP2-WPRE3-BGHpA (Alias: CN2109)

    Plasmid

    #208157

    Purpose

    Direct-expressing SYFP2 in oligodendrocytes AAV Virus

    Depositor

    The Allen Institute for Brain Science, Boaz Levi

    Article

    Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

    Insert

    SYFP2
    

    Use

    AAV

    Tags

    Expression

    Mutation

    NA

    Promoter

    Beta Globin minimal promoter

    Available since

    Oct. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pCMV-M2-TLR2

    Plasmid

    #12291

    Depositor

    Bruce Beutler

    Article

    Jiang et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

    Insert

    TLR2 (Tlr2 Mouse)
    

    Use

    Tags

    FLAG (FLAG-M2)

    Expression

    Mammalian

    Mutation

    M82I and H202Y; signal peptide (aa1-23) replaced …

    Promoter

    Available since

    Jan. 25, 2007

    Availability

    Academic Institutions and Nonprofits only

33. pRRL MND GFP

    Plasmid

    #36247

    Depositor

    David Rawlings

    Article

    Astrakhan et al Blood. 2012 Mar 19.

    Insert

    GFP (eGFP )
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    MND

    Available since

    July 27, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. 1436 pcDNA3 Flag HA

    Plasmid

    #10792

    Purpose

    pcDNA3 vector with added Flag, HA tags

    Depositor

    William Sellers

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 7, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pBS-ldhGFP

    Plasmid

    #27170

    Depositor

    Michela Lizier

    Article

    Lizier et al FEMS Microbiol Lett. 2010 Jul 1. 308(1):

    Insert

    lactate dehydrogenase promoter region fused with modified green fluorescent protein 5
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 24, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pDZ Flag USP28

    Plasmid

    #15665

    Depositor

    Martin Eilers

    Article

    Popov et al Nat Cell Biol. 2007 Jul . 9(7):765-74.

    Insert

    USP28 (USP28 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 2, 2007

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

37. pMT899

    Plasmid

    #228288

    Purpose

    Constitutive mutant rPhlTA expression with strong promoter

    Depositor

    Jun Ishii

    Article

    Tominaga et al Nat Commun. 2024 Dec 19;15(1):10653. doi

    Insert

    rphlTA mutant
    

    Use

    Synthetic Biology

    Tags

    Three copies of VP16 (VP48)-Nuclear localization …

    Expression

    Yeast

    Mutation

    P5S, S6P, K86T, F109L, Q117R, E143K

    Promoter

    KpGAPDH promoter

    Available since

    March 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pQFmcs-2H12.D11

    Plasmid

    #221183

    Purpose

    cdGreen2 expressed from a cumate-inducible promoter (Internal Lab ID: UJ11216)

    Depositor

    Urs Jenal

    Article

    Kaczmarczyk et al Nat Commun. 2024 May 9;15(1):3920. doi:

    Insert

    cdGreen2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PQ5 (cumate-inducible)

    Available since

    Sept. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pTBK1

    Plasmid

    #87443

    Purpose

    express TBK1 in mammalian cells

    Depositor

    Georgios Stathopoulos

    Article

    Marazioti et al Nat Commun. 2018 Feb 14;9(1):672. doi: 1

    Insert

    TBK1 (Tbk1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pMM553

    Plasmid

    #68543

    Purpose

    NanoLuc expressed constitutively from PBT1311, pNBU2 backbone, AmpR

    Depositor

    Timothy Lu, Christopher Voigt

    Article

    Mimee et al Cell Syst. 2015 Jul 29;1(1):62-71. doi:

    Insert

    NanoLuc
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge