We narrowed to 23,940 results for: nes

1. HIR Y1158,1162,1163F

   Plasmid

   #24054

   Depositor

   Frederick Stanley

   Article

   Jacob et al Mol Cell Endocrinol. 2002 Jan 15. 186(1)

   Insert

   Insulin Receptor (INSR Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Y1158F, Y1162F, Y1163F

   Promoter

   Available since

   Dec. 16, 2010

   Availability

   Academic Institutions and Nonprofits only

2. acta1-mRuby2-dGBP

   Plasmid

   #163855

   Purpose

   Zebrafish muscle specific expression of mRuby2-dVHH[GFP]

   Depositor

   Rob Parton

   Article

   Xiong et al Elife. 2021 Feb 16;10. pii: 64631. doi:

   Insert

   dVHH[GFP]
   

   Use

   In vitro transcription of rna

   Tags

   Ruby2

   Expression

   Mutation

   Promoter

   Available since

   Sept. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. S3_001_026_MV2

   Plasmid

   #134422

   Purpose

   pYTKMV2-pTDH3_NLS_FdeR-N_tPGK1-pGPM1-fdeO_mcherry_tTDH1

   Depositor

   Mattheos Koffas

   Article

   Wang et al ACS Synth Biol. 2019 Sep 20;8(9):2121-21

   Inserts

   mCherry
   NLS_FdeR-N
   

   Use

   Tags

   none

   Expression

   Yeast

   Mutation

   none

   Promoter

   pGMP1 and pTDH3

   Available since

   Dec. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHSP70B-CP6-bsr

   Plasmid

   #165878

   Purpose

   Basic Blasticidin resistance cassette in Alpha1, lacks physical marker or attB site

   Depositor

   Koen Venken

   Article

   Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

   Insert

   Basic Selection Vector
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCRISPR_eGFP_497

   Plasmid

   #111824

   Purpose

   Knockout eGFP

   Depositor

   Steve Shih

   Article

   Sinha et al Lab Chip. 2018 Jul 24;18(15):2300-2312.

   Insert

   gRNA targeting eGFP 477-497
   

   Use

   CRISPR

   Tags

   mCherry

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   U6

   Available since

   Aug. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pT3TS-nCas9n-A71

   Plasmid

   #125644

   Purpose

   micro-injection of poly-adenylated nCas9n RNA

   Depositor

   Jason Berman

   Article

   Prykhozhij et al Methods Mol Biol. 2020;2115:385-405. doi

   Insert

   nCas9n
   

   Use

   Rna in vitro transcription vector

   Tags

   Expression

   Mutation

   Promoter

   T3

   Available since

   July 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDEST14-SpyCatcher EQ

   Plasmid

   #35045

   Depositor

   Mark Howarth

   Article

   Zakeri et al Proc Natl Acad Sci U S A. 2012 Mar 20;10

   Insert

   SpyCatcher EQ
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Deletion of C-terminal till I113, and point mutat…

   Promoter

   Available since

   April 18, 2012

   Availability

   Academic Institutions and Nonprofits only

8. px458 EQR

   Plasmid

   #101731

   Purpose

   Expresses a sgRNA and a Cas9 EQR variant that recognizes "NGAG" PAM motifs

   Depositor

   Andrew Holland

   Article

   Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

   Insert

   SpCas9 EQR
   

   Use

   Tags

   3xFLAG-NLS and NLS

   Expression

   Mammalian

   Mutation

   EQR (D1135E, R1335Q, and T1337R mutations in Cas9)

   Promoter

   Available since

   Oct. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pV132-vCas9

   Plasmid

   #203223

   Purpose

   Mammalian expression of vCas9

   Depositor

   Robert Langer, Vikash Chauhan, Phil Sharp

   Article

   Chauhan et al Proc Natl Acad Sci U S A. 2023 Mar 14;12

   Insert

   vCas9
   

   Use

   AAV and CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   S55R R976A K1003A T1314R

   Promoter

   CBh

   Available since

   Aug. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pG3H-U6SC

    Plasmid

    #134755

    Purpose

    pGreen3 CRISPR/Cas9

    Depositor

    Qi-Jun Chen

    Article

    Zhang et al Plant Physiol. 2019 Sep 26. pii: pp.19.0

    Insert

    CRISPR/Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    gRNA-U6, zCas9-35SC

    Available since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pRU293

    Plasmid

    #167688

    Purpose

    Final destination vector for single Gateway LR reaction

    Depositor

    Niko Geldner

    Article

    Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

    Insert

    pEC1.2::Cas9i
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pBa.TfR-Halo

    Plasmid

    #162716

    Purpose

    Mammalian expression of human TFRC

    Depositor

    Marvin Bentley

    Article

    Frank et al Traffic. 2020 Sep 21. doi: 10.1111/tra.1

    Insert

    TfR (TFRC Human) (TFRC Human)
    

    Use

    Tags

    Halo

    Expression

    Mammalian

    Mutation

    none

    Promoter

    Chicken Beta actin

    Available since

    Dec. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMSCV-Pidd1(T788A)

    Plasmid

    #60529

    Purpose

    Retroviral expression of Pidd1 T788A phosphorylation mutant

    Depositor

    Trudy Oliver

    Article

    Terry et al Cell Death Differ. 2014 Oct 10. doi: 10.

    Insert

    Pidd1 (PIDD1 Human)
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Threonine 788 to Alanine*

    Promoter

    Available since

    March 11, 2015

    Availability

    Academic Institutions and Nonprofits only

14. SNAP-23

    Plasmid

    #186686

    Purpose

    Expresses SNAP-23 in E. Coli

    Depositor

    Axel Brunger

    Article

    Lai et al Nature. 2022 Mar;603(7903):949-956. doi:

    Insert

    SNAP23
    

    Use

    Tags

    GST and thrombin site

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. mCerulean3-PTK2-C-10

    Plasmid

    #55441

    Purpose

    Localization: FAK/Focal Adhesions, Excitation: 433, Emission: 475

    Depositor

    Michael Davidson

    Article

    Markwardt et al PLoS One. 2011 . 6(3):e17896.

    Insert

    PTK2 (PTK2 Human)
    

    Use

    Tags

    mCerulean3

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 10, 2014

    Availability

    Academic Institutions and Nonprofits only

16. PylT(UAG) PylS mCherry-P2A-eGFP(150TAG)

    Plasmid

    #174526

    Purpose

    Dual-fluorescence reporter for quantifying PylT(UAG) decoding efficiency

    Depositor

    Yu-Hsuan Tsai

    Article

    Mills et al Cell Rep Methods. 2021 Sep 16;1(6):10007

    Insert

    PylS
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1α

    Available since

    Oct. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGEMT-PT2A-GFP-Tdtomato-iRFP670

    Plasmid

    #111812

    Purpose

    Tricistronic construct: Co-express three fluorescent proteins by P2A and then T2A

    Depositor

    Li Qian

    Article

    Liu et al Sci Rep. 2017 May 19;7(1):2193. doi: 10.

    Inserts

    GFP
    Tdtomato
    iRFP670
    

    Use

    Cloning intermediate

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pBS NL4-3 envFS eGFP RT-D185K/D186L

    Plasmid

    #101332

    Purpose

    pBS NL4-3 envFS eGFP with mutation that disrupts RT catalytic activity

    Depositor

    Jeremy Luban

    Article

    McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

    Insert

    NL4-3 envFS
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    reverse transcriptase D185K/D186L

    Promoter

    Available since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCEP-Puro-TetOn-L1RP-ORF1_R261A_Halo-GFPai

    Plasmid

    #202569

    Purpose

    Expresses an endogenous-like L1RP LINE-1 element with a C-terminal HaloTag on the ORF1 protein with the R261A mutation and a GFP retrotransposition reporter (GFP-AI) in the 3' UTR

    Depositor

    Liam Holt

    Article

    Sil et al Elife. 2023 Apr 28;12:e82991. doi: 10.75

    Insert

    LINE-1
    

    Use

    Tags

    GFP-AI retrotransposition reporter and HaloTag7 o…

    Expression

    Mammalian

    Mutation

    Changed ORF1p Arginine 261 to Alanine

    Promoter

    Available since

    April 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET21(+)-His6-Ubc13

    Plasmid

    #212715

    Purpose

    Bacterial expression of His6-Ubc13

    Depositor

    Helle Ulrich

    Article

    Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

    Insert

    Ubc13
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pH6HTC_CCDC59-Halo

    Plasmid

    #175327

    Purpose

    Production of HaloTag fusion protein

    Depositor

    Claudia Kutter

    Article

    Atanasoai et al bioRxiv 2021.11.08.467743

    Insert

    CCDC59 (CCDC59 Human)
    

    Use

    Cell free expression

    Tags

    HaloTag-6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pXPR_212

    Plasmid

    #133457

    Purpose

    U6 promoter expresses customizable Spyo-guide; EFS promoter expresses SaurCas9 and 2A site provides puromycin resistance

    Depositor

    John Doench

    Article

    DeWeirdt et al Nat Commun. 2020 Feb 6;11(1):752. doi: 1

    Insert

    control guide
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pRS415_pGal-nCas9(H840A)

    Plasmid

    #79616

    Purpose

    Expresses nCas9(H840A) in yeast cells

    Depositor

    Akihiko Kondo

    Article

    Nishida et al Science. 2016 Aug 4. pii: aaf8729.

    Insert

    SpCas9
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    H840A for nickase Cas9

    Promoter

    pGal1

    Available since

    Sept. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pMOD_C3010

    Plasmid

    #91098

    Purpose

    Module C, Promoter: ZmUbi, Gene: GFP (+ BaeI site for GT donor cloning), Terminator: pea rbcsE9

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    GFP (+ BaeI site for GT donor cloning)
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    ZmUbi

    Available since

    July 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pRK5-mFzd6

    Plasmid

    #42258

    Depositor

    Chris Garcia, Jeremy Nathans

    Article

    Yu et al Development. 2012 Dec 1;139(23):4383-94.

    Insert

    Frizzled 6 (Fzd6 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 29, 2013

    Availability

    Academic Institutions and Nonprofits only

26. GFP-mROCK1-delta3

    Plasmid

    #101291

    Purpose

    Mammalian expression of Rock1-GFP for the study of localisation signaling.

    Depositor

    Alpha Yap

    Article

    Priya et al Nat Cell Biol. 2015 Oct;17(10):1282-93.

    Insert

    Rock1 (Rock1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    1-1080 aa

    Promoter

    CMV

    Available since

    Oct. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pcDNA5/FRT/TO DNAJB4

    Plasmid

    #19472

    Depositor

    Harm Kampinga

    Article

    Hageman et al Cell Stress Chaperones. 2008 Aug 7. ():.

    Insert

    DNAJB4 (DNAJB4 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 6, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pH6HTC_HNRNPC-Halo

    Plasmid

    #175306

    Purpose

    Production of HaloTag fusion protein

    Depositor

    Claudia Kutter

    Article

    Atanasoai et al bioRxiv 2021.11.08.467743

    Insert

    HNRNPC (HNRNPC Human)
    

    Use

    Cell free expression

    Tags

    HaloTag-6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. mLPx-shZMPSTE24

    Plasmid

    #69067

    Purpose

    encodes a shRNA against the protease ZMPSTE24

    Depositor

    Gerardo Ferbeyre

    Article

    Moiseeva et al Cell Cycle. 2015 Aug 3;14(15):2408-21. d

    Insert

    shRNA against ZMPSTE24 (ZMPSTE24 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available since

    Jan. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

30. EYFP-GalT

    Plasmid

    #11936

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Cole et al Science. 1996 Aug 9. 273(5276):797-801.

    Insert

    GalT (GALT Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Amino acids 1-60 of the human galactosyltransfera…

    Promoter

    Available since

    Sept. 13, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pSC5

    Plasmid

    #104779

    Purpose

    pSC218GG-Glycine max Ubiquitin:Gateway expression cassette binary vector

    Depositor

    Robert Stupar

    Article

    Curtin et al Plant Biotechnol J. 2017 Oct 31. doi: 10

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Jan. 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pH6HTC_IGF2BP1-Halo

    Plasmid

    #175300

    Purpose

    Production of HaloTag fusion protein

    Depositor

    Claudia Kutter

    Article

    Atanasoai et al bioRxiv 2021.11.08.467743

    Insert

    IGF2BP1 (IGF2BP1 Human)
    

    Use

    Cell free expression

    Tags

    HaloTag-6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pCORE-Kp53

    Plasmid

    #72241

    Purpose

    Contains the CORE cassette with KanMX4 and human p53 gene markers

    Depositor

    Francesca Storici, Michael Resnick

    Article

    Storici et al Genet Eng (N Y). 2003;25:189-207.

    Inserts

    p53 (TP53 Human)
    kanMX4
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    V122A mutation

    Promoter

    Available since

    Jan. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pCaUra3-CUbo-AID*-GFP

    Plasmid

    #212775

    Purpose

    C-terminal PCR-based tagging with CUbo-AID*-yeGFP in budding yeast with CaUra3 selection

    Depositor

    Helle Ulrich

    Article

    Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

    Type

    Empty backbone

    Use

    Pcr-based tagging

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pRTP801/TA-ODD

    Plasmid

    #206212

    Purpose

    An expression vector of hypoxia-responsive transactivator under control of RTP801 promoter

    Depositor

    Masamichi Kamihira

    Article

    Masumoto et al J Biosci Bioeng. 2021 Oct;132(4):399-407

    Insert

    tetracycline-responsive transactivator fused with oxygen-dependent degradation domain
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pRK5-mFzd4

    Plasmid

    #42256

    Depositor

    Chris Garcia, Jeremy Nathans

    Article

    Yu et al Development. 2012 Dec 1;139(23):4383-94.

    Insert

    Frizzled 4 (Fzd4 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 28, 2013

    Availability

    Academic Institutions and Nonprofits only

37. pQTEV-YWHAE

    Plasmid

    #31562

    Depositor

    Konrad Buessow

    Article

    Bussow et al Microb Cell Fact. 2005 Jul 5. 4():21.

    Insert

    tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, epsilon (YWHAE Human)
    

    Use

    Tags

    His and TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 22, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pCS2-SP-FRB (T2098L) -sfGFP-TM-V5

    Plasmid

    #186227

    Purpose

    mRNA synthesis of rapalog-induced cell adhesion molecule

    Depositor

    Yoshihiro Morishita

    Article

    Usami et al Genes Cells. 2022 Jun;27(6):436-450. doi

    Insert

    mRNA synthesis of rapalog-induced cell adhesion molecule
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    FRB (T2098L)

    Promoter

    Available since

    July 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pspCas9-3'UTR-Tetra-com-vector

    Plasmid

    #132553

    Purpose

    Expresses spCas9 and sgRNA scaffold with com aptamer replacing the Tetraloop

    Depositor

    Baisong Lu

    Article

    Lu et al CRISPR J. 2021 Mar 16. doi: 10.1089/cris

    Inserts

    Cas9
    sgRNA scaffold with com replacing the Tetraloop
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pCMB-NUCr

    Plasmid

    #61169

    Purpose

    Fluorescent reporter for nucleus NLS-mCherry-GUS expressed under MtBCP1 promoter

    Depositor

    Maria Harrison

    Article

    Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

    Insert

    NLS-mCherry-GUS
    

    Use

    Tags

    mCherry

    Expression

    Plant

    Mutation

    Promoter

    MtBCP1

    Available since

    Feb. 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge