We narrowed to 19,064 results for: iNOS

1. pNOC-CRISPR-P2A-BlastR

   Plasmid

   #98147

   Purpose

   Expresses Cas9-Nlux-P2A-BlastR and sgRNA in Nannochloropsis oceanica.

   Depositor

   Eva Farre

   Article

   Poliner et al ACS Synth Biol. 2018 Apr 20;7(4):962-968

   Type

   Empty backbone

   Use

   Algae, nannochloropsis expression

   Tags

   P2A-BlastR fusion with Cas9-Nlux

   Expression

   Mutation

   Promoter

   Ribi promoter

   Available Since

   Feb. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. L-GARS-2

   Plasmid

   #166531

   Purpose

   scFv of a human scaffold targeting Glycyl-tRNA synthetase. Antigen coverage aa 55-739 of 739

   Depositor

   Susanne Gräslund

   Article

   Preger et al J Biol Chem. 2020 Oct 9;295(41):13981-13

   Insert

   single chain-Fv, scFv
   

   Use

   Recombinant antibody expression

   Tags

   3xFLAG, His6 and OmpA leader sequence

   Expression

   Bacterial

   Mutation

   Promoter

   LacZ

   Available Since

   March 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-hSyn-sbGLuc-ChR2(CS/DA)-EYFP

   Plasmid

   #114106

   Purpose

   fusion protein of Gaussia luciferase variant slow burn, Channelrhodopsin-2 variant C128S/D156A (stabilized step function opsin), and enhanced yellow fluorescent protein for bioluminescent optogenetics

   Depositor

   Ute Hochgeschwender

   Article

   Berglund et al J Neurosci Res. 2019 Apr 7. doi: 10.1002

   Insert

   Gaussia luciferase, slow burn; Channelrhodopsin 2(CS/DA), stable step function opsin; EYFP
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hSyn

   Available Since

   Aug. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pKUNOC-ErbB1-mScarlet

   Plasmid

   #197360

   Purpose

   Encoding human ErbB1 tagged with mScarlet.

   Depositor

   Michiyuki Matsuda

   Article

   Matsuda et al J Cell Sci. 2023 Jul 31:jcs.261199. doi:

   Insert

   ERBB1 (EGFR Human)
   

   Use

   Tags

   mScarlet

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. AA008

   Plasmid

   #216003

   Purpose

   Fragmid fragment: (Cas protein) preferred over wildtype Cas12a

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Has Service

   Cloning Grade DNA

   Insert

   Cas12a_v1.1 [EnAs]
   

   Use

   CRISPR; Fragment

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRK5-FLAG-Sestrin1 isoform1

   Plasmid

   #72594

   Purpose

   overexpression, codon optimized for mammalian expression

   Depositor

   David Sabatini

   Article

   Chantranupong et al Cell Rep. 2014 Oct 9;9(1):1-8. doi: 10.1

   Insert

   SESN1 (SESN1 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   codon optimized for mammalian expression, see dep…

   Promoter

   CMV

   Available Since

   Feb. 2, 2016

   Availability

   Academic Institutions and Nonprofits only

7. pAAV-hSyn-OsTIR1(WT)

   Plasmid

   #140729

   Purpose

   OsTIR1(WT)-P2A-mAID-EGFP-NES

   Depositor

   Masato Kanemaki

   Article

   Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

   Insert

   OsTIR1(WT)-P2A-mAID-EGFP-NES
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAsCpf1(TATV)(BB) (pY221)

   Plasmid

   #89354

   Purpose

   Expresses humanized AsCpf1 TATV PAM variant and crRNA guide

   Depositor

   Feng Zhang

   Article

   Gao et al Nat Biotechnol. 2017 Jun 5. doi: 10.1038

   Insert

   Cpf1 RVR variant
   

   Use

   CRISPR

   Tags

   NLS and NLS-3xHA

   Expression

   Mammalian

   Mutation

   S542R/K548V/N552R

   Promoter

   CBh

   Available Since

   July 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBbE1k-3

   Plasmid

   #71722

   Purpose

   tyrosine hydroxylase from mouse, dihydropterine reductase from human, pterin-4a-carbinolamine dehydratase from human

   Depositor

   Taek Soon Lee

   Article

   Satoh et al Metab Eng. 2012 Nov;14(6):603-10. doi: 1

   Inserts

   dihydropterine reductase (QDPR Human)
   pterin-4a-carbinolamine dehydratase (PCBD1 Human)
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   pTrc

   Available Since

   April 13, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGEX 4T1 ERbeta

    Plasmid

    #35563

    Depositor

    Harish Srinivas

    Article

    Zhang et al Mol Endocrinol. 2010 Sep;24(9):1737-47.

    Insert

    Estrogen Receptor beta (ESR2 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 26, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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11. CRY2-OCRL(D523G)

    Plasmid

    #79567

    Purpose

    expression of CRY2PHR fused to phosphatase dead mutant of OCRL

    Depositor

    Pietro De Camilli, Olof Idevall-Hagren

    Article

    Idevall-Hagren et al Proc Natl Acad Sci U S A. 2012 Aug 28;10

    Insert

    OCRL (OCRL Human)
    

    Use

    Tags

    CRY2 (photolyase homology region domain of Arabid…

    Expression

    Mammalian

    Mutation

    aa 234_539 and phosphatase mutation, D523G

    Promoter

    CMV

    Available Since

    July 11, 2016

    Availability

    Academic Institutions and Nonprofits only

12. pGEX-4T-1 UBXN2A

    Plasmid

    #113505

    Purpose

    Bacterial expression of GST-tagged UBXN2A

    Depositor

    Hemmo Meyer

    Article

    Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

    Insert

    UBXN2A (UBXN2A Human)
    

    Use

    Tags

    GST-Thrombin cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Oct. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. CIBN-GFP-TMD

    Plasmid

    #79571

    Purpose

    expression of CIBN domain fused to GFP tagged mouse Syntaxin1 TMD domain (transmembrane domain and polybasic region)

    Depositor

    Pietro De Camilli, Olof Idevall-Hagren

    Article

    Idevall-Hagren et al Proc Natl Acad Sci U S A. 2012 Aug 28;10

    Insert

    syntaxin 1A (Stx1a Mouse)
    

    Use

    Tags

    CIBN and EGFP

    Expression

    Mammalian

    Mutation

    aa 249_288 (C-terminal region (polybasic stretch …

    Promoter

    CMV

    Available Since

    July 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRK5-HA-HBXIP

    Plasmid

    #42328

    Depositor

    David Sabatini

    Article

    Bar-Peled et al Cell. 2012 Sep 14;150(6):1196-208. doi:

    Insert

    HBXIP (LAMTOR5 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, SP6

    Available Since

    March 6, 2013

    Availability

    Academic Institutions and Nonprofits only

15. pRK5-FLAG-Sestrin3

    Plasmid

    #72592

    Purpose

    overexpression, codon optimized for mammalian expression

    Depositor

    David Sabatini

    Article

    Chantranupong et al Cell Rep. 2014 Oct 9;9(1):1-8. doi: 10.1

    Insert

    SESN3 (SESN3 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    codon optimized for mammalian expression, see dep…

    Promoter

    CMV

    Available Since

    March 28, 2016

    Availability

    Academic Institutions and Nonprofits only

16. pBSSK p85 alpha (delta iSH2)

    Plasmid

    #13432

    Depositor

    Ronald Kahn

    Article

    Taniguchi et al Proc Natl Acad Sci U S A. 2006 Aug 8. 10

    Insert

    p85 alpha (delta iSH2) (PIK3R1 Human)
    

    Use

    Tags

    flag

    Expression

    Bacterial

    Mutation

    Dominant negative p85 alpha. The p110 alpha bindi…

    Promoter

    Available Since

    Nov. 1, 2006

    Availability

    Academic Institutions and Nonprofits only

17. pT3TS-zAsCpf1

    Plasmid

    #107185

    Purpose

    plasmid to carry out IVT of AsCpf1 (zebrafish codon optimized)

    Depositor

    Antonio Giraldez

    Article

    Moreno-Mateos et al Nat Commun. 2017 Dec 8;8(1):2024. doi: 1

    Insert

    AsCpf1
    

    Use

    CRISPR

    Tags

    NLS-3xHA

    Expression

    Mutation

    Promoter

    Available Since

    March 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBabe puro RalA Q75L

    Plasmid

    #19719

    Depositor

    Channing Der

    Article

    Lim et al Curr Biol. 2006 Dec 19. 16(24):2385-94.

    Insert

    V-ral simian leukemia viral oncogene homolog A (RALA Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    GTPase deficient & constitutively active Gl…

    Promoter

    Available Since

    Oct. 27, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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19. pU6-sgGAL4-1

    Plasmid

    #46915

    Purpose

    Human pSico-based U6 vector containing murine U6 promoter and sgRNA targeting GAL4 UAS promoter

    Depositor

    Jonathan Weissman, Stanley Qi

    Article

    Gilbert et al Cell. 2013 Jul 9. pii: S0092-8674(13)008

    Inserts

    sgGAL4-1
    Puromycin resistance and mCherry
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and U6

    Available Since

    Oct. 1, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. kiCAP-AAV-PAL1C

    Plasmid

    #196685

    Purpose

    Rep/Cap plasmid for the production of PAL1C, an AAV capsid with CNS tropism in macaques.

    Depositor

    Pardis Sabeti

    Article

    Stanton et al Med (N Y). 2022 Nov 15:S2666-6340(22)004

    Insert

    AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
    

    Use

    AAV

    Tags

    Expression

    Mutation

    PTQGTLR insert between amino acids 588 and 589 of…

    Promoter

    p41

    Available Since

    April 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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