163,606 results

1. CRISPRoff-v2.1

   Plasmid

   #167981

   Purpose

   Expresses CRISPRoff-v2.1 (DNMT3A-DNMT3L-XTEN80-dCas9-HA-2xNLS-BFP-KRAB) downstream of the CAG promoter

   Depositor

   Luke Gilbert

   Article

   Nuñez et al Cell. 2021 Apr 7. pii: S0092-8674(21)003

   Insert

   CRISPRoff-v2.1 (DNMT3A-DNMT3L-dCas9-BFP-KRAB)
   

   Use

   Tags

   2xNLS, DNMT3A-DNMT3L, HA, KRAB, and tagBFP

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   May 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. OpenCRISPR-1

   Plasmid

   #221565

   Purpose

   Express OpenCRISPR-1 nuclease in human cells using a CMV promoter

   Depositor

   Ali Madani

   Article

   Ruffolo et al Nature. 2025 Jul 30. doi: 10.1038/s41586

   Insert

   OpenCRISPR-1
   

   Use

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Aug. 28, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pCMV-evoCAST-TniQ

   Plasmid

   #234728

   Purpose

   Engineered human-codon optimized PseTniQ in mammalian cells (for evoCAST)

   Depositor

   David Liu

   Article

   Witte et al Science. 2025 May 15;388(6748):eadt5199.

   Insert

   PseTniQ
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV-evoCAST-eCas8

   Plasmid

   #234727

   Purpose

   Engineered human-codon optimized PseCas8 in mammalian cells (for evoCAST)

   Depositor

   David Liu

   Article

   Witte et al Science. 2025 May 15;388(6748):eadt5199.

   Insert

   ePseCas8
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   April 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. Pink Flamindo

   Plasmid

   #102356

   Purpose

   biosensor for cAMP in mammalian cells

   Depositor

   Tetsuya Kitaguchi

   Article

   Harada et al Sci Rep. 2017 Aug 4;7(1):7351. doi: 10.1

   Insert

   Pink Flamindo
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Oct. 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV-MMLVgag-D3A-3L-dCas9-ZIM3

   Plasmid

   #240531

   Purpose

   Expresses MMLVgag–DNMT3A-3L-dCas9-ZIM3 for producing RENDER-DNMT3A-3L-dCas9-ZIM3

   Depositor

   James Nuñez

   Article

   Xu et al Nat Commun. 2025 Aug 26;16(1):7948. doi:

   Insert

   MMLVgag-D3A-3L-dCas9-ZIM3
   

   Use

   Tags

   FLAG, HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRS415-GPD

   Plasmid

   #183940

   Purpose

   Control vector that has a GPD promoter with no insert

   Depositor

   Michael Downey

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   June 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pATetO 6XHis-Phusion

   Plasmid

   #206210

   Purpose

   Inducible expression of DNA polymerase

   Depositor

   Andrew Ellington

   Article

   Bhadra et al PLoS One. 2018 Aug 15;13(8):e0201681. do

   Insert

   Phusion DNA polymerase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Dec. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. HCT47_pAAV-eHGT_3Xcore_1137m-minBGprom-iCre297T-WPRE3-bGHpA

   Plasmid

   #233564

   Purpose

   Direct-expressing SYFP2 AAV virus

   Depositor

   Tanya Daigle, The Allen Institute for Brain Science

   Article

   Kussick et al Cell Rep. 2025 Jun 24;44(6):115730. doi:

   Insert

   SYFP2
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   minBglobin

   Available Since

   June 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1-Flag

    Plasmid

    #208051

    Purpose

    Empty vector for mammalian expression, insert a Flag tag (with ATG) at XhoI site of pcDNA3.1 (-), xhoI site at 5' of flag tag destroyed, xhoI site at 3' of flag tag retained. XhoI in-frame with Flag

    Depositor

    Quanfu Ma

    Type

    Empty backbone

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA4 myc PGC-1 alpha

    Plasmid

    #10974

    Purpose

    Mammalian expression of PGC-1a with myc and his tags

    Depositor

    Toren Finkel

    Article

    Ichida et al J Biol Chem. 2002 Dec 27. 277(52):50991-

    Insert

    PGC-1 alpha (PPARGC1A Human)
    

    Use

    Tags

    His and Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 5, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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12. Ai9

    Plasmid

    #22799

    Purpose

    Rosa26 targeting vector, Cre-dependent tdtomato expression. See article for more colors.

    Depositor

    Hongkui Zeng

    Article

    Madisen et al Nat Neurosci. 2010 Jan . 13(1):133-40.

    Insert

    CAG-Floxed tdTomato
    

    Use

    Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Dec. 17, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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13. SpyTag003-sfGFP

    Plasmid

    #133454

    Purpose

    Expresses SpyTag003-superfolder GFP in bacterial cytoplasm

    Depositor

    Mark Howarth

    Article

    Keeble et al Proc Natl Acad Sci U S A. 2019 Dec 10. p

    Insert

    SpyTag003-sfGFP
    

    Use

    Tags

    GSGSGS linker and His6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Dec. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. MCP-mCherry

    Plasmid

    #207668

    Purpose

    To express fluorescently labeled MS2 coat binding protein (MCP)

    Depositor

    Christine Mayr

    Article

    Horste et al Mol Cell. 2023 Dec 21;83(24):4509-4523.e

    Insert

    MCP-mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET-29b(+)_NSP4ss_SerM_NucA-His

    Plasmid

    #218190

    Purpose

    Bacterial expression of a periplasmically expressed His-tagged Serratia Marcescens NucA (Benzonase)

    Depositor

    Florence Pojer

    Insert

    nucA
    

    Use

    Tags

    His and NSP4 signal secretion peptide

    Expression

    Bacterial

    Mutation

    codon optimized ecoli residues - 22-266

    Promoter

    T7

    Available Since

    May 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLenti CMV Blast DEST (706-1)

    Plasmid

    #17451

    Purpose

    3rd gen lentiviral Gateway destination vector, expression, CMV promoter, Blast 

    Depositor

    Paul Kaufman, Eric Campeau

    Article

    Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral; Destination vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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17. Tet-pLKO-puro-Scrambled

    Plasmid

    #47541

    Purpose

    Tet inducible scrambled shRNA.

    Depositor

    Charles Rudin

    Article

    Rudin et al Nat Genet. 2012 Oct;44(10):1111-6. doi:

    Insert

    Scrambled shRNA
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    H1

    Available Since

    April 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGADT7-GW

    Plasmid

    #61702

    Purpose

    Gateway compatible Y2H vector - AD

    Depositor

    Yuhai Cui

    Article

    Lu et al Plant J. 2010 Jan;61(2):259-70. doi: 10.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    May 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. LentiCRISPRv2GFP

    Plasmid

    #82416

    Purpose

    3rd generation lentiviral vector expressing GFP alongside Cas9 and an sgRNA cloning site

    Depositor

    David Feldser

    Article

    Walter et al Cancer Res. 2017 Feb 15. pii: canres.215

    Insert

    GFP
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EFS (P2A)

    Available Since

    Sept. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pPlin5-mScarlet3-H (aka mYongHong)

    Plasmid

    #239408

    Purpose

    Expresses Plin5-mScarlet3-H in mammalian cells

    Depositor

    Zhifei Fu

    Article

    Xiong et al Nat Methods. 2025 Apr 17. doi: 10.1038/s

    Insert

    Plin5 (PLIN5 Human)
    

    Use

    Tags

    mScarlet3-H (aka mYongHong)

    Expression

    Mammalian

    Mutation

    M163H in mScarlet3

    Promoter

    CMV

    Available Since

    Aug. 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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