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Addgene

161,439 results

Showing: 541 - 560 of 161439 results
  1. 3XFLAG-NSarQ27G-VMAn-FKBP

    Plasmid
    #70229
    Purpose
    Expresses FLAG tagged N-terminal portion of alpha sarcin with Q27G mutation along with VMA intein for protein splicing
    Depositor
    Insert
    alpha Sarcin (N-terminal portion)
    Use
    Tags
    3XFLAG, FK506- and rapamycin-binding protein (FKB…
    Expression
    Mammalian
    Mutation
    Changed Gln 27 to Gly
    Promoter
    CMV
    Available since
    Nov. 24, 2015
    Availability
    Academic Institutions and Nonprofits only
  2. pSJX022

    Plasmid
    #232325
    Purpose
    Genome editing in Caulobacter crescentus
    Depositor
    Inserts
    sgRNA
    SpCas9M
    Use
    CRISPR
    Tags
    H-arms and cat
    Expression
    Bacterial
    Mutation
    Promoter
    PJ23119 and Pvan
    Available since
    July 14, 2025
    Availability
    Academic Institutions and Nonprofits only
  3. pSJX024

    Plasmid
    #232327
    Purpose
    Genome editing in Caulobacter crescentus
    Depositor
    Inserts
    sgRNA
    SpCas9M
    Use
    CRISPR
    Tags
    H-arms and cat
    Expression
    Bacterial
    Mutation
    Promoter
    PJ23119 and Pxyl
    Available since
    June 16, 2025
    Availability
    Academic Institutions and Nonprofits only
  4. pSJX016

    Plasmid
    #232324
    Purpose
    Genome editing in Caulobacter crescentus
    Depositor
    Inserts
    sgRNA
    SpCas9M
    Use
    CRISPR
    Tags
    H-arms and sfGFP
    Expression
    Bacterial
    Mutation
    Promoter
    PJ23119 and Pvan
    Available since
    June 16, 2025
    Availability
    Academic Institutions and Nonprofits only
  5. pYPQAT31

    Plasmid
    #223403
    Purpose
    T-DNA vector for SpCas9-D10A based A-to-G base editing for dicot plants; NGG PAM; SpCas9D10A-ABE was driven by AtUBQ10 and the sgRNA was driven by AtU3 promoter; Hygromycin for plants selection.
    Depositor
    Insert
    AtUBQ10-ecTadA8e-SpCas9-D10A-AtU3-gRNA scaffold
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Mutation
    Promoter
    Available since
    April 10, 2025
    Availability
    Academic Institutions and Nonprofits only
  6. pYPQAT25

    Plasmid
    #223397
    Purpose
    T-DNA vector for PmCDA1-CBE_V04 based C-to-T base editing for dicot plants; NGG PAM; 5' editing window; PmCDA1-CBE_V04 was driven by AtUBQ10 and sgRNA was driven by AtU3; Hygromycin for plants select.
    Depositor
    Insert
    AtUBQ10-SpCas9D10A-PmCDA-UGI-T2A-MS2-UGI-AtU3-gRNA scaffold 2.0
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Mutation
    Promoter
    Available since
    April 10, 2025
    Availability
    Academic Institutions and Nonprofits only
  7. TFORF2343

    Plasmid
    #142931
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Insert
    MITF (MITF Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Feb. 27, 2025
    Availability
    Academic Institutions and Nonprofits only
Showing: 541 - 560 of 161439 results