165,978 results

1. pTn7-SCOUT13

   Plasmid

   #200993

   Purpose

   pLv1-pUC18R6KT-miniTn7-Tc, TcR, ApR

   Depositor

   Philip Poole

   Article

   Jorrin et al Microbiome. 2024 May 7;12(1):81. doi: 10

   Type

   Empty backbone

   Expression

   Bacterial

   Available Since

   May 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTn7-SCOUT12

   Plasmid

   #200992

   Purpose

   pLv1-pUC18R6KT-miniTn7-Km, KmR, ApR

   Depositor

   Philip Poole

   Article

   Jorrin et al Microbiome. 2024 May 7;12(1):81. doi: 10

   Type

   Empty backbone

   Expression

   Bacterial

   Available Since

   May 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pKM492-Myc-His

   Plasmid

   #109283

   Purpose

   ORBIT integrating plasmid for C-terminal tagging with Myc-His tag

   Depositor

   Kenan Murphy

   Article

   Murphy et al MBio. 2018 Dec 11;9(6). pii: mBio.01467-

   Insert

   attB-Myc-His
   

   Use

   Orbit integrating plasmid

   Available Since

   May 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-hSyn-GRAB_CCK1.0

   Plasmid

   #208673

   Purpose

   Expresses the genetically-encoded fluorescent cholecystokinin (CCK) sensor GRAB_CCK1.0 in neurons

   Depositor

   Yulong Li

   Article

   Wang et al Science. 2023 Nov 17;382(6672):eabq8173.

   Insert

   GPCR activation based cholecystokinin (CCK) sensor GRAB_CCK1.0
   

   Use

   AAV

   Promoter

   hSyn

   Available Since

   Dec. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. rpoDs366C

   Plasmid

   #129688

   Purpose

   Expression of Sigma 70 S366C

   Depositor

   Jeff Gelles

   Article

   Mumm et al bioRxiv 2020

   Insert

   rpoD (rpoD E. coli)
   

   Tags

   His6/thrombin

   Expression

   Bacterial

   Mutation

   C132S C291S C295S S366C

   Available Since

   May 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. CL20_mEGFP_UBXN4_R3HDM1

   Plasmid

   #205950

   Purpose

   Express mEGFP-tagged fusion protein, UBXN4_R3HDM1 from patient-derived sequence

   Depositor

   Richard Kriwacki

   Article

   Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

   Insert

   UBXN4_R3HDM1 (UBXN4, R3HDM1 Human)
   

   Tags

   mEGFP

   Expression

   Mammalian

   Available Since

   Nov. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. p3E-EEA1

   Plasmid

   #109560

   Purpose

   Multisite gateway vector for 5' tagging with early endosome associated 1

   Depositor

   Rob Parton

   Article

   Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

   Insert

   early endosome associated 1 (eea1 Zebrafish)
   

   Use

   Zebrafish plasmids

   Available Since

   Feb. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHAGE pGK hNLRP3-mNG

   Plasmid

   #218643

   Purpose

   Expresses NLRP3 tagged with mNeonGreen in mammalian cells (lentiviral); driven by the weak pGK promoter with blast selection

   Depositor

   Wade Harper

   Article

   Hollingsworth et al bioRxiv 2024.04.19.590338

   Insert

   NLRP3 (NLRP3 Human)
   

   Use

   Lentiviral

   Tags

   mNeonGreen

   Expression

   Mammalian

   Promoter

   pGK

   Available Since

   May 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTR-177 pUC19-CLTA-N-mCherry

   Plasmid

   #186128

   Purpose

   knockin of mCherry to CLTA target

   Depositor

   Alexander Marson

   Article

   Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

   Insert

   CLTA (CLTA Human)
   

   Use

   CRISPR

   Mutation

   WT with mCherry sequence

   Available Since

   July 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. SRM

    Plasmid

    #25490

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    SRM (SRM Human)
    

    Tags

    His

    Expression

    Bacterial

    Available Since

    Aug. 23, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

11. pEBG-MKK4-S255D/T259E (GST)

    Plasmid

    #21566

    Depositor

    Leonard Zon

    Article

    Sanchez et al Nature. . 372(6508):794-8.

    Insert

    mitogen-activated protein kinase kinase 4 (Map2k4 Mouse)
    

    Tags

    GST

    Expression

    Mammalian

    Mutation

    Serine 255 to Aspartic Acid, Threonine 259 to Gl…

    Available Since

    July 17, 2009

    Availability

    Academic Institutions and Nonprofits only

12. pUBQ10-YFP-GW

    Plasmid

    #209362

    Purpose

    Gateway cloning compatible binary vector for agrobacterium mediated transformation. Arabidopsis UBQ10 promoter driving expression of N-terminal YFP fusion protein.

    Depositor

    Lucia Strader

    Article

    Michniewicz et al BMC Res Notes. 2015 Mar 3;8:63. doi: 10.

    Type

    Empty backbone

    Expression

    Plant

    Available Since

    Jan. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. VAPB_Halo_C_allele

    Plasmid

    #178174

    Purpose

    Donor vector for endogenous tagging of human VAPB at the C-terminus with halotag

    Depositor

    iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

    Insert

    Halotag (VAPB Human)
    

    Use

    Donor vector

    Available Since

    Dec. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLV-mCherry-FKBP-TIAM1(DHPH)

    Plasmid

    #176138

    Purpose

    Heterodimerization with FRB (rapamycin), Rac1 activation

    Depositor

    Dorus Gadella

    Article

    Mahlandt et al Elife. 2023 Jul 14;12:RP84364. doi: 10.7

    Insert

    TIAM1 (TIAM1 Human)
    

    Use

    Lentiviral

    Available Since

    Oct. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTR-374 tNGFR-P2A-B2M

    Plasmid

    #186068

    Purpose

    Knockin of truncated NGFR to target gene

    Depositor

    Alexander Marson

    Article

    Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

    Insert

    B2M (B2M Human)
    

    Use

    CRISPR

    Mutation

    WT with tNGFR sequence

    Available Since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBbS8k-RFP_R43W_E93G

    Plasmid

    #109406

    Purpose

    RepA Mutant

    Depositor

    Jay Keasling

    Article

    Thompson et al Sci Rep. 2018 Jan 25;8(1):1590. doi: 10.

    Insert

    repA
    

    Tags

    RFP

    Expression

    Bacterial

    Mutation

    R43W and E93G repA mutations to increase copy num…

    Available Since

    June 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRetroX-TRE3G_NT-GSDMD_I105N_

    Plasmid

    #201174

    Purpose

    Dox-inducible expression of NT-GSDMD gene in mammalian cells by retroviral transduction

    Depositor

    Jonathan Kagan

    Article

    Devant et al Cell Rep. 2023 Jan 31;42(1):112008. doi:

    Insert

    Gasdermin D N-terminal domain (Gsdmd Mouse)
    

    Use

    Retroviral

    Mutation

    aa1-276 only, I105N

    Promoter

    TRE3G

    Available Since

    May 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. P2RY8-Tango

    Plasmid

    #66477

    Purpose

    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

    Depositor

    Bryan Roth

    Article

    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

    Insert

    P2RY8 (P2RY8 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

19. pEntr1a-mcherry-FKBP-TIAM1(DHPH)

    Plasmid

    #176135

    Purpose

    Heterodimerization with FRB (rapamycin), Rac1 activation

    Depositor

    Dorus Gadella

    Article

    Mahlandt et al Elife. 2023 Jul 14;12:RP84364. doi: 10.7

    Insert

    TIAM1 (TIAM1 Human)
    

    Use

    Entry vector

    Mutation

    See Depositor Comments Below

    Available Since

    Oct. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCIBN-LacI

    Plasmid

    #103814

    Purpose

    BLInCR 'Localizer' construct that marks lacO arrays and is targeted by a PHR-tagged effector upon illumination with blue light

    Depositor

    Karsten Rippe

    Article

    Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

    Insert

    CIBN-LacI (CIB1, lacI Mustard Weed, E. coli)
    

    Expression

    Mammalian

    Mutation

    LacI: C19T (silent, L7L), T264C (silent, A88A), C…

    Promoter

    CMV

    Available Since

    Jan. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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