159,238 results

1. pLenti-EF1a-Ngn3ER-PuroR-mCherry

   Plasmid

   #197385

   Purpose

   To establish Ngn3ER cell line for endocrine differentiation (4-hydroxytamoxifen activation, Puromycin resistant)

   Depositor

   Qiao Zhou

   Article

   Huang et al Nat Cell Biol. 2023 Apr 27. doi: 10.1038

   Insert

   Ngn3ER-2A-PuroR-2A-mCherry
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1A

   Available since

   March 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. T4 td upstream intron-PABP

   Plasmid

   #189972

   Purpose

   Part 1 plasmid to introduce the T4 td upstream intron and PABP spacer into circRNAs

   Depositor

   Howard Chang

   Article

   Chen et al Nat Biotechnol. 2022 Jul 18. pii: 10.103

   Insert

   T4 td upstream intron-PABP
   

   Use

   Cloning

   Tags

   Expression

   Mutation

   Promoter

   N/A

   Available since

   Sept. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3-flag-YTHDF2

   Plasmid

   #52300

   Purpose

   full length YTHDF2 with N-terminal flag tag for mammalian cell expression

   Depositor

   Chuan He

   Article

   Wang et al Nature. 2014 Jan 2;505(7481):117-20. doi

   Insert

   YTHDF2 (YTHDF2 Human)
   

   Use

   Tags

   flag

   Expression

   Mammalian

   Mutation

   Promoter

   T7

   Available since

   April 14, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. HBA1 full 3'UTR-T4 td downstream intron

   Plasmid

   #189974

   Purpose

   Part 6 plasmid to introduce the HBA1 full 3'UTR and T4 td downstream intron into circRNAs

   Depositor

   Howard Chang

   Article

   Chen et al Nat Biotechnol. 2022 Jul 18. pii: 10.103

   Insert

   HBA1 full 3'UTR-T4 td downstream intron
   

   Use

   Cloning

   Tags

   Expression

   Mutation

   Promoter

   N/A

   Available since

   Sept. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. AAV-mOXT-hM3Dq-mCherry-WPRE

   Plasmid

   #70717

   Purpose

   Expresses DREADDs Gq receptor under oxytocin promoter control

   Depositor

   Daniel Geschwind

   Article

   Penagarikano et al Sci Transl Med. 2015 Jan 21;7(271):271ra

   Insert

   hM3Dq-mCherry
   

   Use

   AAV

   Tags

   mCherry

   Expression

   Mutation

   Promoter

   mouse oxytocin

   Available since

   Jan. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLVX-Puro-TDP-43-Q331K

   Plasmid

   #133761

   Purpose

   Lentiviral expression of human TDP-43 Q331K disease mutant in mammalian cells.

   Depositor

   Shawn Ferguson

   Article

   Roczniak-Ferguson et al Life Sci Alliance. 2019 Sep 16;2(5). pii

   Insert

   TDP-43 (TARDBP Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Changed glutamine 331 to lysine.

   Promoter

   CMV

   Available since

   Dec. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. M3-PAmcherry1

   Plasmid

   #114189

   Purpose

   studying clustering of M3 receptors by superresolution microscopy (PALM)

   Depositor

   Aleksandra Radenovic

   Insert

   Muscarin acetylcholine receptor 3 (CHRM3 Human)
   

   Use

   Tags

   3xHA and PAmCherry

   Expression

   Mammalian

   Mutation

   Promoter

   SV40

   Available since

   Oct. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pPB-cT3G-cERP2-HMGB2

   Plasmid

   #222532

   Purpose

   PiggyBac transposon plasmid for doxycycline inducible expression of HMGB2

   Depositor

   George Church

   Article

   Pierson Smela et al bioRxiv 2024.05.31.596483

   Insert

   HMGB2 (HMGB2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pIS2

   Plasmid

   #12177

   Depositor

   David Bartel

   Article

   Farh et al Science. 2005 Dec 16. 310(5755):1817-21.

   Type

   Empty backbone

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 3, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSBbi-GB

    Plasmid

    #60520

    Purpose

    SB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: GFP and blasticidin resistance gene

    Depositor

    Eric Kowarz

    Article

    Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b

    Type

    Empty backbone

    Use

    Transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a/RPBSA

    Available since

    Feb. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. ptdTomato-SMCR8

    Plasmid

    #83438

    Purpose

    Expresses SMCR8-tdTomato in mammalian cells

    Depositor

    Shawn Ferguson

    Article

    Amick et al Mol Biol Cell. 2016 Aug 24. pii: mbc.E16

    Insert

    Human SMCR8 (SMCR8 Human)
    

    Use

    Tags

    tdTomato

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFB2

    Plasmid

    #195282

    Purpose

    Bicistronic DTR and Puromycin resistance for positive/negative selection

    Depositor

    Scott Lowe

    Article

    Barriga et al Nat Cancer. 2022 Nov;3(11):1367-1385. do

    Insert

    DTR 2A Puro (HBEGF Human, Synthetic)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available since

    Jan. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pT3-EF1aH Yap S127A

    Plasmid

    #86497

    Purpose

    Mammalian expression of FLAG-tagged Yap containing S127A mutation

    Depositor

    Xin Chen

    Article

    Tschaharganeh et al Gastroenterology. 2013 Jun;144(7):1530-1

    Insert

    Yes associated protein 1 (YAP1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    S127A

    Promoter

    EF1a

    Available since

    Feb. 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. EK0479 CMV-TO-VEGF-A(165) (FLP-IN)

    Plasmid

    #233384

    Purpose

    Secreted human VEGF-A

    Depositor

    Xiaojing Gao

    Article

    Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

    Insert

    VEGF-A (VEGFA Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    CMV

    Available since

    April 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. Ac5-STABLE2-neo

    Plasmid

    #32426

    Depositor

    Rosa Barrio, James Sutherland

    Article

    Gonzalez et al Sci Rep. 2011;1:75. doi: 10.1038/srep000

    Insert

    Flag-mCherry-T2A-GFP-T2A-neo
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Actin5C

    Available since

    Oct. 6, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGGAselect

    Plasmid

    #195714

    Purpose

    pGGAselect is a cloning vector for Golden Gate Assembly using BsaI, BsmBI, and BbsI Type IIS restriction enzymes.

    Depositor

    Eric Cantor

    Article

    Potapov et al ACS Synth Biol. 2018 Nov 16;7(11):2665-2

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 24, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. Gq-CASE

    Plasmid

    #168125

    Purpose

    Encodes a BRET-based activity sensor for the heterotrimeric G protein Gq. Composed of the subunits G alpha q (GNAQ) tagged with NanoLuciferase, G beta 3 (GNB3) and Venus-tagged G gamma 9 (GNG9).

    Depositor

    Gunnar Schulte

    Article

    Schihada et al Sci Signal. 2021 Sep 7;14(699):eabf1653.

    Insert

    GNAQ, GNB3, GNG9 (GNAQ, GNB3, GNGT2 Human)
    

    Use

    Luciferase

    Tags

    cpVenus on GNG9

    Expression

    Mammalian

    Mutation

    NLuc is inserted at F124/E125 within GNAQ

    Promoter

    Available since

    July 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. PB53x EF1-Dendra2-RPB1Amr

    Plasmid

    #81228

    Purpose

    expresses Dendra2-Rpb1 fusion in mammalian cells. Possible to insert into the genome using the Piggibac system

    Depositor

    Timothée Lionnet

    Article

    Cho et al Elife. 2016 May 3;5. pii: e13617. doi: 1

    Insert

    Dendra2 - Rpb1 (alpha amanitin resistant) (POLR2A Human)
    

    Use

    Tags

    Dendra2

    Expression

    Mammalian

    Mutation

    N792D alpha amanitin resistance mutation (Bartolo…

    Promoter

    EF1a

    Available since

    Nov. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLKO-p53-shRNA-427

    Plasmid

    #25636

    Depositor

    Todd Waldman

    Article

    Kim et al Mol Cell Biol. 2007 Jan . 27(2):662-77.

    Insert

    p53-shRNA-427 (TP53 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 21, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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20. pT3-EF1aH NICD1

    Plasmid

    #86500

    Purpose

    Mammalian expression of Myc tagged NICD1

    Depositor

    Xin Chen

    Article

    Fan et al J Clin Invest. 2012 Aug 1;122(8):2911-5.

    Insert

    Notch1 (C-term) (Notch1 Mouse)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Cytoplasmic domain only (AA 1753-2531)

    Promoter

    EF1a

    Available since

    April 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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21. EGFP-Tau(T217E)

    Plasmid

    #187024

    Purpose

    Expresses EGFP tagged human 2N4R tau (with T217E mutation) in mammalian cells with CMV promoter

    Depositor

    George Bloom

    Article

    Rajbanshi et al Alzheimers Dement. 2023 Jan 12. doi: 10.

    Insert

    MAPT (MAPT Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Changed Threonine 217 to Glutamic acid

    Promoter

    CMV

    Available since

    Jan. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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22. pDONR221-SLC25A5_STOP

    Plasmid

    #161173

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC25A5 (SLC25A5 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 21, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

23. lentiCRISPRv2FE-ABE8e-SpRY

    Plasmid

    #213008

    Purpose

    A lentiviral vector expressing the ABE8e-SpRY base editor and an sgRNA cloning site

    Depositor

    Richard Sherwood

    Article

    Ryu et al Nat Genet. 2024 Apr 24. doi: 10.1038/s41

    Insert

    ABE8e-SpRY-D10A
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    D10A nickase variant of SpRY

    Promoter

    Ef-1a

    Available since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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24. pBSTNAV

    Plasmid

    #45801

    Depositor

    Luc Ponchon

    Article

    Ponchon et al Nucleic Acids Res. 2013 Aug;41(15):e150.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. mRNA-CleanCap entry vector

    Plasmid

    #188249

    Purpose

    Cloning vector for generating template plasmids to transcribe mRNA, with mNeonGreen dropout following BsaI digestion

    Depositor

    Howard Chang

    Article

    Chen et al Nat Biotechnol. 2022 Jul 18. pii: 10.103

    Insert

    mNeonGreen
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    T7

    Available since

    Sept. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. cytoplasmic cpVenus control

    Plasmid

    #186788

    Purpose

    Identical to cytoplasmic NAD+ biosensor, sans bipartite NAD+-binding domain modeled from bacterial DNA ligase

    Depositor

    Michael Cohen

    Article

    Cambronne et al Science. 2016 Jun 17;352(6292):1474-7. d

    Insert

    cytoplasmic cpVenus control
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pCG-Hc∆18-AA

    Plasmid

    #86559

    Purpose

    Plasmid encodes blinded measles virus H glycoprotein

    Depositor

    Jakob Reiser

    Article

    Ou et al Hum Gene Ther Methods. 2012 Apr;23(2):13

    Insert

    Measles virus H glycoprotein
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Y463A, R515A

    Promoter

    Available since

    Feb. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. synIRES_RC25

    Plasmid

    #189970

    Purpose

    Part 2 plasmid to introduce the HRV-B3 IRES with proximal loop Apt-eIF4G into circRNAs

    Depositor

    Howard Chang

    Article

    Chen et al Nat Biotechnol. 2022 Jul 18. pii: 10.103

    Insert

    synIRES_RC25
    

    Use

    Cloning

    Tags

    Expression

    Mutation

    Promoter

    N/A

    Available since

    Sept. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. lenti SYN-SVI-DIO-dCas9-VPR

    Plasmid

    #164576

    Purpose

    Expresses a SYN-driven, intron-containing dCas9-VPR fusion in which the part of the dCas9 cassette is double floxed and in inverted orientation (DIO). Requires Cre-dependent recombination.

    Depositor

    Jeremy Day

    Article

    Carullo et al eNeuro. 2021 Jul 26. pii: ENEURO.0188-21

    Insert

    A DIO FLAG-dCas9-VPR cassette containing an SV40 intron
    

    Use

    CRISPR, Cre/Lox, and Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. PGK-EGFP-FKBPF36V

    Plasmid

    #199765

    Purpose

    plasmid for targeting dTAG into the AAVS1 locus

    Depositor

    Hannes Lans

    Article

    van der Woude et al Nucleic Acids Res. 2023 Oct 13;51(18):e9

    Insert

    GFP-dTAG
    

    Use

    Tags

    GFP and dTAG

    Expression

    Mammalian and Worm

    Mutation

    Promoter

    hPGK

    Available since

    June 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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