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We narrowed to 1,640 results for: plasmid for e coli

Showing: 1351 - 1400 of 1640 results

  17. pcDNA3-HLA-cMyc-EcopT1R1

    Plasmid
    #113962
    Purpose
    mammalian expression plasmid for c-myc-tagged E. coli codon-optimized human T1R1 with signal peptide of HLA class I histocompatibility antigen A-2 alpha chain
    Depositor
    Erik Procko
    Article
    Park et al J Biol Chem. 2019 Feb 5. pii: RA118.0061
    Insert
    T1R1 (TAS1R1 Human)
    Use
    Tags
    Signal/leader sequence from HLA class I histocomp…
    Expression
    Mammalian
    Mutation
    truncate N-terminal 24 residues
    Promoter
    Available since
    Feb. 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    113962-plasmid-map-sequence-id-223994

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  20. pYES2-gRNA-hyg-MCS

    Plasmid
    #107734
    Purpose
    E. coli-S. cerevisiae shuttle plasmid harbors gRNA expression cassettes targeting S. cerevisiae chromosomal X-3 site (HXK1 gene). Can function as a gRNA expression empty backbone plasmid.
    Depositor
    Sheng Yang
    Article
    Wang et al Biotechnol Bioeng. 2018 Sep 10. doi: 10.
    Insert
    X-3 site gRNA expression cassettes
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    SNR52p
    Available since
    Jan. 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    107734-plasmid-map-sequence-id-208539

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  23. 3'-ecRESCUE-mCherry

    Plasmid
    #191486
    Purpose
    ecDHFR DD-mCherry, an inducible ecRESCUE RNA base editor: TMP-induced ecDHFR DD expression allows reversible control of the expression of dRanCas13b-ADAR2-mCherry
    Depositor
    Xu Ma
    Article
    Wang et al Cell Commun Signal. 2021 Jul 31;19(1):81
    Insert
    dRanCas13b-hADAR2-mCherry-ecDHFR (folA, ADARB1 R. anatipestifer, E. coli, Human)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Oct. 19, 2022
    Availability
    Academic Institutions and Nonprofits only
    191486-plasmid-map-sequence-id-374351

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  27. pCJ203

    Plasmid
    #162678
    Purpose
    pET-21b(+) based plasmid for expression of the putative MHET hydrolase from Comomonas thiooxydans (Genbank WP_080747404.1) with deletion of the predicted signal peptide (Phe2:Ala75)
    Depositor
    Gregg Beckham
    Article
    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11
    Insert
    Putative MHETase from Comomonas thiooxydans (Genbank WP_080747404.1) without 75 residue signal peptide
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    deltaF2-A75; codon optimized for expression in E.…
    Promoter
    T7/lac
    Available since
    Feb. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    162678-plasmid-map-sequence-id-321524

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  34. pKM197

    Plasmid
    #132665
    Purpose
    pyrE-targeted CRISPR-Cas9 control plasmid. Cas9 expression is controlled by the xylR promoter and results in improved conjugation efficiency. Induction with xylose results in a pyrE mutant.
    Depositor
    Joseph Sorg
    Article
    Bhattacharjee et al mSphere. 2020 Mar 4;5(2):e00941-19. doi:
    Insert
    Upstream & Downstream pyrE deletion region
    Use
    E. coli - c. difficile shuttle vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 17, 2019
    Availability
    Academic Institutions and Nonprofits only
    132665-plasmid-map-sequence-id-259679

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  36. Plas-CRISPR_reporter

    Plasmid
    #157658
    Purpose
    CRISPR reporter for mRNA quantification, integrative plasmid into NPR2 gene
    Depositor
    Frank Albert
    Article
    Brion et al Elife. 2020 Nov 16;9. pii: 60645. doi: 1
    Insert
    dCAS9-VP64, mCherry, KanMX
    Use
    Insert storage (replicative in e. coli)
    Tags
    Expression
    Mutation
    N28D in mCherry- please see depositor comments be…
    Promoter
    Available since
    Sept. 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    157658-plasmid-map-sequence-id-309095

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  37. pRL1058

    Plasmid
    #70686
    Purpose
    Tn5-based plasmid for transposition in Anabaena sp. strain PCC 7120. Bears an oriV, so when excised from a chromosome or megaplasmid it can carry its neighboring sequences when returned to E. coli.
    Depositor
    Peter Wolk
    Article
    Wolk et al Proc Natl Acad Sci U S A. 1991 Jun 15;88
    Insert
    Transposon Tn5-1058
    Use
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    70686-plasmid-map-sequence-id-126440

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  41. pEPQD0KN0244

    Plasmid
    #162282
    Purpose
    Acceptor for golden gate assembly with BsaI overhangs GCTT-CCAT, adds a N-terminal expression tag. Generates plasmids for T7-driven E. coli cell-free protein synthesis
    Depositor
    Nicola Patron
    Article
    Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    162282-plasmid-map-sequence-id-320851

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  42. pEPQD0KN0024

    Plasmid
    #162281
    Purpose
    Acceptor for golden gate assembly with BsaI overhangs GCTT-AATG, adds a N-terminal expression tag. Generates plasmids for T7-driven E. coli cell-free protein synthesis
    Depositor
    Nicola Patron
    Article
    Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    162281-plasmid-map-sequence-id-320849

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  44. pAP114

    Plasmid
    #120799
    Purpose
    E. coli-C. difficile shuttle vector for xylose-inducible expression in C. difficile; Pxyl driving expression of red fluorescent protein (mCherryOpt)
    Depositor
    David S. Weiss, Craig Ellermeier
    Article
    Muh et al J Bacteriol. 2019 Feb 11. pii: JB.00711-
    Insert
    Pxyl::mCherryOpt
    Use
    Tags
    Expression
    Bacterial
    Mutation
    1.4 kb xylR-Pxyl DNA fragment from C. difficile R…
    Promoter
    Pxyl
    Available since
    Feb. 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    120799-plasmid-map-sequence-id-235314

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  46. pGDP4 tet(A)

    Plasmid
    #112887
    Purpose
    This plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Plac promoter.
    Depositor
    Gerard Wright
    Article
    Cox et al Cell Chem Biol. 2017 Jan 19;24(1):98-109
    Insert
    tet(A)
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Sequence was codon optimized for E. coli K12 usin…
    Promoter
    Plac
    Available since
    Dec. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    112887-plasmid-map-sequence-id-219894

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Showing: 1351 - 1400 of 1640 results