We narrowed to 19,041 results for: Cre

1. hsa-cdk2 3'UTR sicheck2 mut

   Plasmid

   #121124

   Purpose

   luciferase assay

   Depositor

   Qing Deng

   Article

   Hsu et al Proc Natl Acad Sci U S A. 2019 Aug 26. p

   Insert

   hsa-cdk2 3'UTR mutated 199 binding (CDK2 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   miR-199 seed binding site mutated

   Promoter

   Available since

   Sept. 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. Tol2-lyzC-mcherry-2a-CDK2-D145N

   Plasmid

   #121132

   Purpose

   neutrophil specific expression

   Depositor

   Qing Deng

   Article

   Hsu et al Proc Natl Acad Sci U S A. 2019 Aug 26. p

   Insert

   dre-D145N CDK2 DN (cdk2 Zebrafish)
   

   Use

   Tags

   mcherry-2a

   Expression

   Bacterial

   Mutation

   D145N point mutation DN

   Promoter

   neutrophil specific lyzC

   Available since

   Sept. 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. dre-cdk2 3'UTR sicheck2 199 mut

   Plasmid

   #121122

   Purpose

   luciferase assay

   Depositor

   Qing Deng

   Article

   Hsu et al Proc Natl Acad Sci U S A. 2019 Aug 26. p

   Insert

   dre-cdk2 3'UTR mutated 199 binding (cdk2 Zebrafish)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   miR-199 seed binding site mutated

   Promoter

   Available since

   Sept. 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pKLV2-U6gRNA5(Slc25a51-g1)-PGKpuroBFP-W

   Plasmid

   #105028

   Purpose

   Lentiviral gRNA plasmid targeting mouse Slc25a51 , co-expression of TagBFP

   Depositor

   Kosuke Yusa

   Article

   Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

   Insert

   Slc25a51 (Slc25a51 Mouse)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Sept. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pKLV2-U6gRNA5(Slc25a51-g2)-PGKpuroBFP-W

   Plasmid

   #105029

   Purpose

   Lentiviral gRNA plasmid targeting mouse Slc25a51 , co-expression of TagBFP

   Depositor

   Kosuke Yusa

   Article

   Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

   Insert

   Slc25a51 (Slc25a51 Mouse)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Sept. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pKLV2-U6gRNA5(Pou5f1-g1)-PGKpuroBFP-W

   Plasmid

   #105035

   Purpose

   Lentiviral gRNA plasmid targeting mouse Pou5f1 , co-expression of TagBFP

   Depositor

   Kosuke Yusa

   Article

   Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

   Insert

   Pou5f1 (Pou5f1 Mouse)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Sept. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pKLV2-U6gRNA5(Kcmf1-g1)-PGKpuroBFP-W

   Plasmid

   #105017

   Purpose

   Lentiviral gRNA plasmid targeting mouse Kcmf1 , co-expression of TagBFP

   Depositor

   Kosuke Yusa

   Article

   Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

   Insert

   Kcmf1 (Kcmf1 Mouse)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Sept. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. 289aa Met133, 138, 186Ileu ELL2

   Plasmid

   #127272

   Purpose

   Expresses 289aa human ELL2 with Met133, 138, 186Ileu

   Depositor

   Christine Milcarek

   Article

   Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

   Insert

   ELL2 w Met to ILeus stopped at XbaI (ELL2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Met133, 138, 186 Ileu, cut with XbaI blunt fillin

   Promoter

   pEF1a

   Available since

   July 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. frameshift ELL2

   Plasmid

   #127257

   Purpose

   For in vitro translation of human a frameshift ELL2 that results in a 58 kDa product from internal initiation.

   Depositor

   Christine Milcarek

   Article

   Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

   Insert

   ELL2 (ELL2 Human)
   

   Use

   In vitro translation

   Tags

   Expression

   Mutation

   XhoI site between the 1st &2nd Met was filled…

   Promoter

   T7

   Available since

   July 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. 564aa ELL2 bluescript

    Plasmid

    #127267

    Purpose

    For in vitro translation of human ELL2 lacking COOH region

    Depositor

    Christine Milcarek

    Article

    Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

    Insert

    ELL2 (ELL2 Human)
    

    Use

    In vitro translation

    Tags

    Expression

    Mutation

    Addgene Plasmid 127256--SphI site filled in, then…

    Promoter

    T7

    Available since

    July 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. KA901: pMVP (L3-L2) HA tag + pA; EF1a::eGFP-P2A-TETa-pA

    Plasmid

    #121804

    Purpose

    pMVP L3-L2 entry plasmid, contains HA tag-polyA + EF1a-eGFP-P2A-TETa-polyA for 3- or 4-component MultiSite Gateway Pro assembly. Adds C-term HA tag to gene plus downstream EF1a-driven GFP-P2A-TETa

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    HA epitope tag-polyA + EF1a::eGFP-P2A-TETa-polyA
    

    Use

    Synthetic Biology; Pmvp gateway entry plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. KA701: pMVP (L3-L2) HA tag + pA; RIP::eGFP-P2A-TETa-pA

    Plasmid

    #121808

    Purpose

    pMVP L3-L2 entry plasmid, contains HA tag-polyA + RIP-eGFP-P2A-TETa-polyA for 3- or 4-component MultiSite Gateway Pro assembly. Adds C-term HA tag to gene plus downstream RIP-driven GFP-P2A-TETa

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    HA epitope tag-polyA + RIP::eGFP-P2A-TETa-polyA
    

    Use

    Synthetic Biology; Pmvp gateway entry plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. KB701: pMAGIC (L5-L4) hU6::SaCas9 gRNA scaffold; CMV promoter

    Plasmid

    #121818

    Purpose

    pMAGIC L5-L4 entry plasmid, contains empty human U6-driven SaCas9 gRNA scaffold and CMV promoter for 4-component MultiSite Gateway Pro assembly. Protospacer motif can be inserted after BsaI digestion

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Type

    Empty backbone

    Use

    Synthetic Biology; Pmagic gateway entry plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. EF1a_KLF8_P2A_Hygro_Barcode

    Plasmid

    #120494

    Purpose

    Barcoded lentiviral vector to express KLF8 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

    Depositor

    Prashant Mali

    Article

    Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

    Insert

    KLF8 (KLF8 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Feb. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pFUSEss-CHIg-mG3_CH2charge

    Plasmid

    #105858

    Purpose

    Expression plasmid coding for mutated heavy chain of mouse IgG3 antibody specific to antigen B of the ABO blood group system, clone M18. Introduced mutatnions modify charge of CH2 domain.

    Depositor

    Joanna Bereta

    Article

    Klaus et al Front Immunol. 2018 May 23;9:1096. doi:

    Insert

    IgG3 heavy chain (Ighg3 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    mutated heavy chain of mouse IgG3, muatations: Hi…

    Promoter

    hEF1-HTLV prom

    Available since

    March 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCIneoRL-Notch1 3'UTR-mir200c-mut

    Plasmid

    #84596

    Purpose

    Renilla Luciferase reporter assay for NOTCH1 3'UTR with mutations on miR-200c binding site

    Depositor

    Yanan Yang

    Article

    Zhang et al Nat Commun. 2016 Jul 26;7:12231. doi: 10

    Insert

    NOTCH1 3'UTR (NOTCH1 Human)
    

    Use

    Luciferase

    Tags

    Renilla Luciferase

    Expression

    Mutation

    Mutation on miR-200c binding site on NOTCH1 3…

    Promoter

    CMV

    Available since

    Nov. 4, 2016

    Availability

    Academic Institutions and Nonprofits only

17. pCIneoRL-LFNG 3'UTR-mir200c-mut

    Plasmid

    #84594

    Purpose

    Renilla Luciferase reporter assay for LFNG 3'UTR with mutations on miR-200c binding site

    Depositor

    Yanan Yang

    Article

    Zhang et al Nat Commun. 2016 Jul 26;7:12231. doi: 10

    Insert

    LFNG 3'UTR (LFNG Human)
    

    Use

    Luciferase

    Tags

    Renilla Luciferase

    Expression

    Mutation

    Mutation on miR-200c binding site on LFNG 3'…

    Promoter

    CMV

    Available since

    Nov. 4, 2016

    Availability

    Academic Institutions and Nonprofits only

18. Lrrc4c-AP-His

    Plasmid

    #71959

    Purpose

    Expresses the extracellular region of the LRRC4C protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Lrrc4c (Lrrc4c Mouse)
    

    Use

    Tags

    AP-His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. LXSN-Cx43-S279A

    Plasmid

    #60705

    Purpose

    retroviral expression of rat Cx43 S279A mutant

    Depositor

    Parmender Mehta

    Article

    Johnson et al Mol Biol Cell. 2013 Mar;24(6):715-33. do

    Insert

    Cx43 (Gja1 Rat)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    S279A

    Promoter

    LTR

    Available since

    July 6, 2015

    Availability

    Academic Institutions and Nonprofits only

20. pcDNA-Cx43-S279/282A

    Plasmid

    #60712

    Purpose

    mammalian expression of rat Cx43 S279/282A mutant

    Depositor

    Parmender Mehta

    Article

    Johnson et al Mol Biol Cell. 2013 Mar;24(6):715-33. do

    Insert

    Cx43 (Gja1 Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    S279/282A

    Promoter

    CMV

    Available since

    July 6, 2015

    Availability

    Academic Institutions and Nonprofits only

21. pcDNA-Cx43-S279/282D

    Plasmid

    #60714

    Purpose

    mammalian expression of rat Cx43 S279/282D mutant

    Depositor

    Parmender Mehta

    Article

    Johnson et al Mol Biol Cell. 2013 Mar;24(6):715-33. do

    Insert

    Cx43 (Gja1 Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    S279/282D

    Promoter

    CMV

    Available since

    July 6, 2015

    Availability

    Academic Institutions and Nonprofits only

22. FNE2-A72

    Plasmid

    #44608

    Depositor

    Robert Oshima

    Article

    Galang et al J Biol Chem. 1996 Apr 5;271(14):7992-8.

    Insert

    Ets2-A72 (Ets2 Mouse)
    

    Use

    Tags

    FLAG and SV40 NLS

    Expression

    Mammalian

    Mutation

    THR-72 converted to Alanine

    Promoter

    CMV

    Available since

    April 26, 2013

    Availability

    Academic Institutions and Nonprofits only

23. KHBD00295

    Plasmid

    #34421

    Depositor

    Bart Deplancke

    Article

    Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

    Insert

    CG12052 (lola Fly)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 23, 2012

    Availability

    Academic Institutions and Nonprofits only

24. KHBD00321

    Plasmid

    #34446

    Depositor

    Bart Deplancke

    Article

    Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

    Insert

    CG8676 (Hr39 Fly)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 23, 2012

    Availability

    Academic Institutions and Nonprofits only

25. KHBD00050

    Plasmid

    #34212

    Depositor

    Bart Deplancke

    Article

    Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

    Insert

    CG11502 (svp Fly)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 23, 2012

    Availability

    Academic Institutions and Nonprofits only

26. KHBD00060

    Plasmid

    #34221

    Depositor

    Bart Deplancke

    Article

    Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

    Insert

    CG9415 (Xbp1 Fly)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 23, 2012

    Availability

    Academic Institutions and Nonprofits only

27. KHBD00012

    Plasmid

    #34174

    Depositor

    Bart Deplancke

    Article

    Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

    Insert

    CG8328 (HLHmdelta Fly)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 23, 2012

    Availability

    Academic Institutions and Nonprofits only

28. LentiGuide Blast

    Plasmid

    #199622

    Purpose

    Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and blasticidin resistance from EF-1a promoter.

    Depositor

    Danwei Huangfu

    Article

    Luo et al Nat Genet. 2023 Aug;55(8):1336-1346. doi

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pOGS539_haPD-1

    Plasmid

    #226716

    Purpose

    Plasmid enabling yeast-mediated expression and secretion of high affinity PD-1 microbody (haPD-1)

    Depositor

    Gautam Dantas

    Article

    Rebeck et al Cell Chem Biol. 2024 Nov 16:S2451-9456(2

    Insert

    High affinity PD-1 microbody
    

    Use

    Tags

    HA tag and Mating factor alpha secretion signal

    Expression

    Yeast

    Mutation

    Promoter

    pTEF1

    Available since

    Dec. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pOGS539_rPD-L1nb

    Plasmid

    #226717

    Purpose

    Plasmid enabling yeast-mediated expression and secretion of recombinant PD-L1 nanobody (rPD-L1nb)

    Depositor

    Gautam Dantas

    Article

    Rebeck et al Cell Chem Biol. 2024 Nov 16:S2451-9456(2

    Insert

    Recombinant PD-L1 nanobody
    

    Use

    Tags

    HA tag, Histidine tag, and Mating factor alpha se…

    Expression

    Yeast

    Mutation

    Promoter

    pTEF1

    Available since

    Dec. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. scAAV-sgRNA-GFP

    Plasmid

    #177935

    Purpose

    Expresses sgRNA and can be packaged into AAV particles for somatic delivery into Cas9 transgenic mice

    Depositor

    Roland Rad

    Article

    Kaltenbacher et al Nat Protoc. 2022 Apr;17(4):1142-1188. do

    Type

    Empty backbone

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Nov. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. mEmerald-Sec61b-C1

    Plasmid

    #90992

    Purpose

    Expresses mEmerald-tagged Sec61b, brightly labels endoplasmic reticulum in mammalian cells

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Nixon-Abell et al Science. 2016 Oct 28;354(6311). pii: aaf

    Insert

    mEmerald-Sec61b (SEC61B Synthetic, Human)
    

    Use

    Tags

    mEmerald (green fluorescence)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pT4-U6-sgRNA-CMV-EGFP

    Plasmid

    #239587

    Purpose

    Sleeping-beauty based EV for cloning and expression of sgRNAs - concomitant expression of GFP serves as transfection marker

    Depositor

    Andreas Moor

    Article

    Borrelli et al Nature. 2024 Aug;632(8024):411-418. doi:

    Type

    Empty backbone

    Use

    Sleeping beauty transposone

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    June 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. mCherry-Sec61b-C1

    Plasmid

    #90994

    Purpose

    Expresses mCherry-tagged Sec61b, brightly labels endoplasmic reticulum in mammalian cells

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Nixon-Abell et al Science. 2016 Oct 28;354(6311). pii: aaf

    Insert

    mCherry-Sec61b (SEC61B Synthetic, Human)
    

    Use

    Tags

    mCherry (red fluorescence)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. W118-1_hTGM1-Flag

    Plasmid

    #180406

    Purpose

    lentiviral transduction of human TGM1 (with a C-terminal Flag tag) gene

    Depositor

    Huda Zoghbi

    Article

    Lee et al J Clin Invest. 2022 May 2;132(9). pii: 1

    Insert

    TGM1 (TGM1 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. Lenti‐Cas9‐2A‐Blast

    Plasmid

    #73310

    Purpose

    Lentiviral vector expressing human codon-optimized S. pyogenes FLAG-tagged Cas9 and blasticidin resistance from EFS promoter

    Depositor

    Jason Moffat

    Article

    Hart et al Cell. 2015 Dec 3;163(6):1515-26. doi: 10

    Insert

    Cas9
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    EFS-NS

    Available since

    Feb. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pEGFP-C1-AR V7

    Plasmid

    #86856

    Purpose

    Fluorescent human androgen-receptor splice variant 7, lacking the ligand-binding domain (fused to EGFP)

    Depositor

    Marco Marcelli, Michael Mancini

    Article

    Szafran et al Prostate. 2017 Jan;77(1):82-93. doi: 10.

    Insert

    Androgen receptor (AR) (AR Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Alternative splice variant 7 (alteration/deletion…

    Promoter

    CMV

    Available since

    April 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pLVX-ATF4 mScarlet NLS

    Plasmid

    #115969

    Purpose

    ER-stress sensor - ATF4 translation at ORF3 fluorescent reporter with mScarlet-I

    Depositor

    David Andrews

    Article

    Nougarede et al Assay Drug Dev Technol. 2018 Aug 8. doi:

    Insert

    activating transcription factor 4 (ATF4 Synthetic, Human)
    

    Use

    Lentiviral

    Tags

    HA tag, c-myc NLS, and mScarlet-I

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. CXCR3-DuET

    Plasmid

    #213220

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    CXCR3 (CXCR3 Human)
    

    Use

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, T7

    Available since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pmCherry-2xFYVE

    Plasmid

    #140050

    Purpose

    Expresses mCherry-tagged HRS 2xFYVE in mammalian cells

    Depositor

    Harald Stenmark

    Article

    Sneeggen et al Nat Commun. 2019 Jun 28;10(1):2850. doi:

    Insert

    tandem HRS FYVE domain (Hgs Mouse)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    two FYVE domains (amino acids 147-223), linked by…

    Promoter

    CMV

    Available since

    May 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge