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Plasmid#104495PurposeAAV-mediated expression of ultrasensitive protein calcium sensor under the CAG promoter, Cre mediated flip-excision switchDepositorHas ServiceAAV PHP.eB, AAV Retrograde, and AAV1InsertjGCaMP7s
UseAAV and Cre/LoxTagsT7 epitope, Xpress tag, 6xHisExpressionMammalianMutationPromoterCAGAvailable sinceDec. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDEST17-His10-ccdB
Plasmid#166145PurposeGateway destination vector for generation of N-terminal His10-tagged proteins in E. coli.DepositorArticleTypeEmpty backboneUseTagsHis10ExpressionBacterialMutationPromoterAvailable sinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRC_RR_VP1_r1c3
Plasmid#65724PurposeRescued infectious variant from pAAV2_RaPID library; mCherry insertion in place of the deleted amino acid sequence 453-GTTTQSRDepositorInsertmCherry
UseAAVTagsExpressionMutationPromoterAvailable sinceAug. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCDB179-DepoPETase
Plasmid#218702PurposeEncodes codon optimized DepoPETase enzyme for bacterial expressionDepositorInsertDepoPETase
UseTags10xHisExpressionBacterialMutationPromoterAvailable sinceMay 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-PE2-SpRY-P2A-GFP
Plasmid#159981PurposePrime editing in mammalian cellsDepositorInsertPE2-SpRY-P2A-GFP
UseTagsBpSV40 NLSExpressionMammalianMutationPromoterCMVAvailable sinceNov. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
MBP-FUS_FL_WT
Plasmid#98651PurposeExpresses MBP-tagged full length FUSDepositorInsertFUS (FUS Human)
UseTags6xHis-MBPExpressionBacterialMutationPromoterAvailable sinceJuly 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLenti_CMV_CapChR2-GFP
Plasmid#224357PurposeExpresses CapChR2-GFP in mammalian cellsDepositorInsertCapChR2
UseLentiviralTagsExpressionMutationPromoterAvailable sinceNov. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
IFNGR2_pcDNA6.2/EmGFP-Bsd
Plasmid#176939PurposeMammalian expression vector encoding IFNGR2 and EmGFP-BsdDepositorInsertIFNGR2 (IFNGR2 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti6-DEST PINK1-V5 KD
Plasmid#13319DepositorInsertPINK1 (PINK1 Human)
UseLentiviralTagsV5ExpressionMammalianMutationKinase dead: K219A/D362A/D384APromoterAvailable sinceOct. 16, 2006AvailabilityAcademic Institutions and Nonprofits only -
p53 (dominant negative R175H mutant)-pcw107-V5
Plasmid#64638Purpose3rd generation lentiviral transfer plasmid. When used to produce lentivirus, express physiological levels of insertDepositorInsertTP53 (transcript variant 3) (TP53 Human)
UseLentiviralTagsV5ExpressionMutationR175HPromoterPGKAvailable sinceDec. 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
EMTB-3XGFP
Plasmid#26741DepositorInsertensconsin (MAP7 Human)
UseProbe for microtubulesTags3x EGFP and MycExpressionMammalianMutationOnly contains the microtubule binding-domain of e…PromoterAvailable sinceFeb. 24, 2011AvailabilityAcademic Institutions and Nonprofits only -
pLGB36
Plasmid#135621PurposeSuicide vector for allelic replacement in Bacteroides species including B. fragilis strain 638R, erythromycin selection and aTC-inducible ss-Bfe3 counterselectionDepositorInsertbfe3
UseBacteroides suicide vector with inducible counter…Tagssignal sequence for periplasmic targetingExpressionMutationPromoterBacteroides promoter regualted by TetR transcript…Available sinceJan. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pINDUCER21-MYB
Plasmid#51305PurposeExpression of MYB after doxycycline administrationDepositorInsertv-myb avian myeloblastosis viral oncogene homolog (MYB Human)
UseLentiviralTagsn/aExpressionMutationfull length ORFPromoterAvailable sinceJune 13, 2014AvailabilityAcademic Institutions and Nonprofits only -
AiP12408 - pAAV-AiE0600m-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2408)
Plasmid#191728PurposeAI plasmid ALIAS: AiP12408. Expression of SYFP2 (yellow fluorescent protein) in striatal and neocortical Sst-Chodl neuronsHas ServiceAAV PHP.eBInsertSYFP2
UseAAVTagsExpressionMutationnonePromoterAvailable sinceFeb. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET21 gamma-tubulin
Plasmid#101825Purposeproduction of gamma-tubulin in bacteriaDepositorInsertgamma-tubulin 1 (TUBG1 Human)
UseTagsHisExpressionBacterialMutationWhole proteinPromoterT7Available sinceOct. 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMV-PE7-mutant (Q20A_Y23A_Y24F_F35A)
Plasmid#224123PurposeMammalian expression of SpCas9 PE7 mutant prime editor (Q20A_Y23A_Y24F_F35A)DepositorInsertPE7-mutant (Q20A_Y23A_Y24F_F35A)
UseTagsSV40 bpNLS and c-Myc NLSExpressionMammalianMutationQ20A_Y23A_Y24F_F35A in La(SSB)1-194PromoterCMVAvailable sinceAug. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPAGFP-C1
Plasmid#11910DepositorHas ServiceCloning Grade DNAInsertPAGFP
UseTagsExpressionMammalianMutationThese plasmids are essentially the same as the Cl…PromoterAvailable sinceJuly 3, 2008AvailabilityAcademic Institutions and Nonprofits only -
CMV-Citron1
Plasmid#134303PurposeMammalian expression of direct-response citrate sensorDepositorInsertCitron1
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
hSyn1-sypHy-RGECO
Plasmid#84078PurposeCombined fluorescent reporter for both presynaptic calcium influx and vesicle excoytosisDepositorInsertsypHy-RGECO
UseTagsExpressionMammalianMutationPromoterhSyn1Available sinceNov. 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJYDC1
Plasmid#162458PurposeYeast display plasmid for expression of C-terminal fusions with Aga2p yeast agglutinin. The reporter, eUnaG2 bilirubin dependent green-yellow fluorescent protein, is fused to the NterminusDepositorTypeEmpty backboneUseShuttle vector bacteria/yeastTagsHA tag, HDEL sequence, eUnaG2 fluorescent protein…ExpressionMutationPromoterAvailable sinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFREE_Zeo
Plasmid#92053PurposeAllows inducible expression of gRNAs and Cas9 for plasmid curing.DepositorInsertsCas9
gRNA array
UseCRISPRTagsExpressionMutationPromoterpRhamBAD and ptetAvailable sinceJune 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSMALB-ATF4.12
Plasmid#155033PurposeFluorescent reporter for ATF4 translation, Negative ControlDepositorInsertsUseLentiviralTagsExpressionMammalianMutationuORF1 start codon mutatedPromoterAvailable sinceSept. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAGM4723
Plasmid#48015PurposePlease see depositor comments section.DepositorTypeEmpty backboneUseUnspecifiedTagsExpressionMutationPromoterAvailable sinceDec. 3, 2013AvailabilityIndustry, Academic Institutions, and Nonprofits -
pEGFP-VSP4-E228Q
Plasmid#80351PurposeExpression of GFP-tagged, dominant negative VPS4 mutantDepositorInsertVPS4-E228Q (VPS4A Human)
UseTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceJan. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCW57.1-FOXM1b
Plasmid#68811PurposeDoxycycline inducible lentiviral vector for FOXM1b expression in mammalian cellsDepositorInsertFOXM1b (FOXM1 Human)
UseLentiviral; Doxycycline inducibleTagsHAExpressionMammalianMutationPromoterTRE promoter, Tet ONAvailable sinceSept. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMS2M-PH-gagpol-D64V
Plasmid#166031PurposeExpression of bacteriophage-derived MS2 coat protein fused HIV-1 Lentiviral Gag-Pol at N terminal with a HIV-1 protease cleavage signal between MS2 and Gag-Pol.DepositorInsertMS2-Gag-Pol
UseLentiviralTagsExpressionMutationD64V mutation within the integrase within PolPromoterAvailable sinceMarch 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBabe-EGFP-Hu_Plk1(WT)
Plasmid#136323PurposeRetroviral expression of human Plk1-WT with N-terminal EGFPDepositorInsertPLK1 (PLK1 Human)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
FUGW-PK-hLC3
Plasmid#61460PurposeExpress pHluorin-mKate2-hLC3 (PK-hLC3) in mammalian cells for monitoring autophagy, based on FUGW (3rd gen lentiviral plasmid)DepositorInsertPK-hLC3 (MAP1LC3B Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTriEx-mCherry-LOV2 V416L
Plasmid#81036Purposemammalian expression of LOV V416L, a mutant with super long relaxation timeDepositorInsertLOV V416L
UseTagsmCherryExpressionBacterial, Insect, and Mamm…MutationV416LPromoterAvailable sinceAug. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
ODD-Luciferase-pcDNA3
Plasmid#18965DepositorInsertODD-Firefly Luciferase (HIF1A Human)
UseTagsExpressionMammalianMutationHIF1a ODD sequence fused in frame to luciferase O…PromoterAvailable sinceAug. 20, 2008AvailabilityAcademic Institutions and Nonprofits only