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178,736 results

Showing: 8401 - 8430 of 178736 results

  1. AICSDP-36: AAVS1-mEGFP (PGK)

    Plasmid
    #114404
    Purpose
    Homology arms and linker-mEGFP sequence for internal insertion of PGK-mEGFP at the AAVS1 safe harbor location in human cells as a reporter for cytoplasmic mEGFP
    Depositor
    Allen Institute for Cell Science
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    AAVS1 Homology Arms with PGK driven mEGFP (AAVS1 Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Mutation
    homology arms contain point mutations to disrupt …
    Available since
    Sept. 4, 2018
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    114404-plasmid-map-sequence-id-220815

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  2. pLM494

    Plasmid
    #100539
    Purpose
    CEN/ARS plasmid harboring expression cassettes for crtI, crtYB, crtE and tHMG1 of the β-carotene pathway, each flanked by loxPsym sites.
    Depositor
    Bernd Müller-Röber
    Publication
    Hochrein et al Nat Commun. 2018 May 22;9(1):1931. doi:
    Inserts
    GGPP synthase
    phytoene desaturase
    phytoene-beta carotene synthase
    HMG CoA reductase
    Use
    Cre/Lox and Synthetic Biology
    Expression
    Yeast
    Promoter
    PGK1, TDH3, TIP1, and ZEO1
    Available since
    June 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    100539-plasmid-map-sequence-id-194476

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  3. pBad-CA

    Plasmid
    #105665
    Purpose
    expresses thermostable variant of human carbonic anhydrase II with C-terminal 6x his tag
    Depositor
    Ryan Mehl
    Publication
    Mehl et al 2020
    Insert
    human carbonic anhydrase II thermostable variant (CA2 Human)
    Tags
    6x his tag
    Expression
    Bacterial
    Promoter
    pBad/arabinose
    Available since
    March 14, 2018
    Availability
    Academic Institutions and Nonprofits only
    105665-plasmid-map-sequence-id-205511

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  7. pHR-EF1a-dCas9-HA-BFP-KRAB-NLS

    Plasmid
    #102244
    Purpose
    Lentiviral expression of dCas9-HA-BFP-KRAB-NLS for CRISPRi driven by a hEF1alpha promoter
    Depositor
    Jacob Corn
    Insert
    dCas9-HA-BFP-KRAB-NLS
    Use
    CRISPR and Lentiviral
    Tags
    HA-TagBFP-KRAB-NLS
    Expression
    Mammalian
    Promoter
    EF1a
    Available since
    Oct. 25, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    102244-plasmid-map-sequence-id-197289

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  11. pSAM_Cam

    Plasmid
    #91570
    Purpose
    Mariner transposon mutagenesis & sequencing vector - Transposon contains KanR and Illumina sequencing adapters. Lac promoter expresses transposase. Chloramphenicol resistance on backbone.
    Depositor
    Harry Mobley
    Publication
    Bachman et al MBio. 2015 Jun 9;6(3):e00775. doi: 10.11
    Type
    Empty backbone
    Expression
    Bacterial
    Available since
    Aug. 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by

  17. pDule-tfmF A65V S158A

    Plasmid
    #85484
    Purpose
    Machinery plasmid expressing permissive Mj tfmF synthetase and cognate amber suppressing tRNA
    Depositor
    Ryan Mehl
    Publication
    Miyake-Stoner et al Biochemistry. 2010 Mar 2;49(8):1667-77.
    Insert
    tri-fluoromethyl-phenylalanine Mj synthetase A65V S158A
    Tags
    None
    Expression
    Bacterial
    Mutation
    Y32V F108W Q109M I159M with 65Val and 158Ala
    Promoter
    lpp (constitutive)
    Available since
    Jan. 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    85484-plasmid-map-sequence-id-169864

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  20. RHCglo

    Plasmid
    #80169
    Purpose
    Minigene reporter for identification and analysis of cis elements and trans factors affecting pre-mRNA splicing
    Depositor
    Thomas Cooper
    Publication
    Singh et al Biotechniques. 2006 Aug;41(2):177-81.
    Insert
    segments of chicken cardiac troponin T (Tnnt2) introns 4 and 5 flanking an artifical exon
    Expression
    Mammalian
    Available since
    Sept. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    80169-plasmid-map-sequence-id-150808

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  27. pEGFP-C3-PLK4-3xFLAG

    Plasmid
    #69837
    Purpose
    This plasmid encodes PLK4 isoform 1 with an N-terminal EGFP tag and C-terminal 3xFLAG tag
    Depositor
    Michel Bornens
    Publication
    Sillibourne et al Mol Biol Cell. 2010 Feb 15;21(4):547-61.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PLK4 (PLK4 Human)
    Tags
    3xFLAG tag and EGFP
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Oct. 21, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    69837-plasmid-map-sequence-id-123876

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  29. pmCherry-C1 actin-3XNLS P2A mCherry

    Plasmid
    #58475
    Purpose
    Expresses nuclear-targeted human actin, with an mCherry expression reporter after a P2A protease cleavage site, on a CMV promoter
    Depositor
    Dyche Mullins
    Publication
    Belin et al Elife. 2015 Aug 19;4. doi: 10.7554/eLife
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    beta actin (ACTB Human)
    3XNLS
    P2A cleavage site
    Tags
    mCherry
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Sept. 18, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    58475-plasmid-map-sequence-id-90385

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  30. PX408 Francisella tularensis subsp. novicida Cas9

    Plasmid
    #68705
    Purpose
    Francisella tularensis subsp. novicida Cas9
    Depositor
    Feng Zhang
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    FtCas9
    Use
    CRISPR
    Tags
    HA-NLS
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Aug. 25, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    68705-plasmid-map-sequence-id-140302

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Showing: 8401 - 8430 of 178736 results