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159,461 results

Showing: 8401 - 8430 of 159461 results

  2. pMSCV-LTR-dCas9-VP64-BFP

    Plasmid
    #46912
    Purpose
    Human expression vector containing MSCV LTR promoter, dCas9 that is fused to 2x NLS, VP64 and tagBFP
    Depositor
    Stanley Qi, Jonathan Weissman
    Article
    Gilbert et al Cell. 2013 Jul 9. pii: S0092-8674(13)008
    Inserts
    dCas9-VP64-BFP fusion
    Puromycin resistance
    Use
    CRISPR and Retroviral
    Tags
    3xNLS, BFP, and VP64 domain
    Expression
    Mammalian
    Mutation
    Promoter
    LTR and PGK
    Available since
    Oct. 1, 2013
    Availability
    Academic Institutions and Nonprofits only
    46912-plasmid-map-sequence-id-66610

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  3. TFORF0662

    Plasmid
    #143063
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    CHD6 (CHD6 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    June 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    143063-plasmid-map-sequence-id-442319

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  8. TFORF0096

    Plasmid
    #142459
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    ZEB2 (ZEB2 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Dec. 2, 2024
    Availability
    Academic Institutions and Nonprofits only
    142459-plasmid-map-sequence-id-454978

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  10. plex_307-ccdB-blasticidin

    Plasmid
    #201136
    Purpose
    pLEX_307 plasmid with blasticidin marker
    Depositor
    Alejandro Chavez
    Type
    Empty backbone
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF-1α
    Available since
    June 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    201136-plasmid-map-sequence-id-395507

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  14. CMV-eGFP-Z1

    Plasmid
    #135049
    Purpose
    Enhanced Green fluorescent protein expression for mammalian cells under constitutive CMV Promoter in minimal backbone plasmid.
    Depositor
    Jordan Green
    Insert
    eGFP
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV Promoter
    Available since
    May 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    135049-plasmid-map-sequence-id-269408

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  16. CDH5-AbR-GFP

    Plasmid
    #122970
    Purpose
    Lenti plasmid containing a CDH5 promoter driving GFP followed by T2A and an antibiotic resistance cassette (zeocin). Used for selection of endothelial cells.
    Depositor
    Budd A. Tucker
    Inserts
    CDH5p
    CopGFP
    Zeocin
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    NA
    Promoter
    Available since
    May 15, 2019
    Availability
    Academic Institutions and Nonprofits only
    122970-plasmid-map-sequence-id-244602

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  21. FH-Tet2-pEF

    Plasmid
    #41710
    Purpose
    This plasmid expresses mouse Tet2 with N-terminal FLAG and HA tags in a mammalian system
    Depositor
    Anjana Rao
    Article
    Ko et al Nature. 2010 Dec 9;468(7325):839-43. doi
    Insert
    tet methylcytosine dioxygenase 2 (Tet2 Mouse)
    Use
    Tags
    FLAG and HA
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Feb. 18, 2014
    Availability
    Academic Institutions and Nonprofits only
    41710-plasmid-map-sequence-id-322448

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  22. pHR-UCOE-SFFV-dCas9-KRAB(Kox1)-MeCP2-P2A-EGFP

    Plasmid
    #188902
    Purpose
    dCas9 with a C-term NLS-KRAB(Kox1)-MeCP2 fusion (no linker), and GFP linked by a P2A site from a SFFV promoter with an upstream ubiquitous chromatin opening element
    Depositor
    Marco Jost, Jonathan Weissman
    Article
    Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75
    Insert
    dCas9-KRAB(Kox1)-MeCP2
    Use
    Lentiviral
    Tags
    NLS-KRAB(Kox1)-MeCP2 fusion (no linker) and P2A-G…
    Expression
    Mutation
    Promoter
    SFFV
    Available since
    Sept. 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    188902-plasmid-map-sequence-id-372712

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  26. pMSP3545

    Plasmid
    #46888
    Purpose
    Gram-Positive Bacterial Shuttle Vector for Nisin-Controlled Inducible Expression
    Depositor
    Gary Dunny
    Article
    Bryan et al Plasmid. 2000 Sep;44(2):183-90.
    Type
    Empty backbone
    Use
    Nisin-inducible expression; gram-positive bacteri…
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PnisA
    Available since
    Sept. 18, 2013
    Availability
    Academic Institutions and Nonprofits only
    46888-plasmid-map-sequence-id-347882

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  27. FokI-dCas9

    Plasmid
    #52970
    Purpose
    Expresses Fok1 nuclease domain fused to catalytically inactive Cas9 DNA-binding domain in mammalian cells
    Depositor
    David Liu
    Article
    Guilinger et al Nat Biotechnol. 2014 Apr 25. doi: 10.103
    Insert
    Fok1 fused to dCas9
    Use
    CRISPR
    Tags
    Fok1 nuclease (homodimeric wildtype) and NLS
    Expression
    Mammalian
    Mutation
    D10A and H840A in Cas9
    Promoter
    CMV
    Available since
    May 21, 2014
    Availability
    Academic Institutions and Nonprofits only
    52970-plasmid-map-sequence-id-82408

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  28. pJOG1336

    Plasmid
    #167303
    Purpose
    Level 0 module (3U+Ter) for further assemblies based on the Modular Cloning system. 3'UTR and flanking sequence of a Solanum lycopersicum Oleosin gene, arbitrarily called OLE2 (Sollyc03g112440)
    Depositor
    Johannes Stuttmann
    Article
    Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1
    Insert
    3'UTR and Terminator SlOLE2
    Use
    Synthetic Biology; Moclo level 0 vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    May 20, 2021
    Availability
    Academic Institutions and Nonprofits only
    167303-plasmid-map-sequence-id-333334

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  29. pJOG1334

    Plasmid
    #167302
    Purpose
    Level 0 module (Pro+5U) for further assemblies based on the Modular Cloning system. Promoter and 5'UTR of a Solanum lycopersicum Oleosin gene, arbitrarily called OLE2 (Sollyc03g112440)
    Depositor
    Johannes Stuttmann
    Article
    Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1
    Insert
    Promoter SlOLE2
    Use
    Synthetic Biology; Moclo level 0 vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    May 20, 2021
    Availability
    Academic Institutions and Nonprofits only
    167302-plasmid-map-sequence-id-333330

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  30. RelB cFlag pcDNA3

    Plasmid
    #20017
    Depositor
    Stephen Smale
    Insert
    RelB (Relb Mouse)
    Use
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 28, 2009
    Availability
    Academic Institutions and Nonprofits only
    20017-plasmid-map-sequence-id-197332

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Showing: 8401 - 8430 of 159461 results