We narrowed to 13,867 results for: cas9

1. PXPR007 sgCIC-1

   Plasmid

   #74953

   Purpose

   Cas9 + sgCIC-1 with blasticidin selection

   Depositor

   William Hahn

   Article

   Wang et al Cell Rep. 2017 Feb 7;18(6):1543-1557. do

   Insert

   CIC (CIC Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   May 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pODN-mNG-RHOA

   Plasmid

   #183836

   Purpose

   Repair template for the N-terminal tagging of RhoA with mNeonGreen in human cells using CRISPR/Cas9.

   Depositor

   Alisa Piekny

   Article

   Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

   Insert

   RHOA homology arms with mNeonGreen-linker (RHOA Human)
   

   Use

   CRISPR; Donor template

   Tags

   mNeonGreen-linker

   Expression

   Mammalian

   Mutation

   Homology arms contain point mutations to remove t…

   Available Since

   May 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. CMV_Npu-BE3.9max N-terminal

   Plasmid

   #137171

   Purpose

   Expresses the N-terminal split-intein fragment of BE3.9max from the CMV promoter

   Depositor

   David Liu

   Article

   Levy et al Nat Biomed Eng. 2020 Jan;4(1):97-110. do

   Insert

   Npu-BE3.9max_N-terminal
   

   Expression

   Mammalian

   Mutation

   Cas9 D10A

   Promoter

   CMV

   Available Since

   Jan. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. CMV_Npu-ABEmax N-terminal

   Plasmid

   #137173

   Purpose

   Expresses the N-terminal split-intein fragment of ABEmax from the CMV promoter

   Depositor

   David Liu

   Article

   Levy et al Nat Biomed Eng. 2020 Jan;4(1):97-110. do

   Insert

   Npu-ABEmax_N-terminal
   

   Expression

   Mammalian

   Mutation

   Cas9 D10A

   Promoter

   CMV

   Available Since

   Jan. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. LENTICRISPR-TKT_sgRNA2

   Plasmid

   #201625

   Purpose

   CRISPR/Cas9-mediated gene knock-out

   Depositor

   Vamsi Mootha, Alexis Jourdain

   Article

   Skinner et al Nat Metab. 2023 May;5(5):765-776. doi: 1

   Insert

   TKT (TKT Human)
   

   Use

   CRISPR and Lentiviral

   Available Since

   July 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. LENTICRISPR-TKT_sgRNA1

   Plasmid

   #201624

   Purpose

   CRISPR/Cas9-mediated gene knock-out

   Depositor

   Vamsi Mootha, Alexis Jourdain

   Article

   Skinner et al Nat Metab. 2023 May;5(5):765-776. doi: 1

   Insert

   TKT (TKT Human)
   

   Use

   CRISPR and Lentiviral

   Available Since

   July 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLentiCRISPR-v1-sgGLS_2

   Plasmid

   #163459

   Purpose

   lentiviral vector expressing Cas9 and an sgRNA targeting GLS

   Depositor

   Jason Cantor

   Article

   Rossiter et al Cell Metab. 2021 Feb 23. pii: S1550-4131

   Insert

   sgRNA 2 targeting GLS (GLS Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Jan. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pODN-mR2-ACTB

   Plasmid

   #183868

   Purpose

   Repair template for the N-terminal tagging of beta-actin with mRuby2 in human cells using CRISPR/Cas9.

   Depositor

   Alisa Piekny

   Article

   Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

   Insert

   ACTB homology arms with mRuby2-linker (ACTB Human)
   

   Use

   CRISPR; Donor template

   Tags

   mRuby2-linker

   Expression

   Mammalian

   Mutation

   Homology arms contain point mutations to remove t…

   Available Since

   May 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. AA025

   Plasmid

   #216005

   Purpose

   Fragmid fragment: (Cas protein) NG PAM

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Has Service

   Cloning Grade DNA

   Insert

   Cas9-NG_v1.1 [Sp]
   

   Use

   CRISPR; Fragment

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. plenti-px330-CRBN-T2-pGK-Pur

    Plasmid

    #107383

    Purpose

    Mammalian expression CRISPR/Cas9

    Depositor

    William Kaelin

    Article

    Lu et al Science. 2014 Jan 17;343(6168):305-9. do

    Insert

    sgRNA targeting CRBN (CRBN Human)
    

    Use

    Lentiviral; Crispr

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    June 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. AAVS1 KanR foundation lox casette

    Plasmid

    #132388

    Purpose

    Foundation cassette containing lox sites for cre-mediated exchange of inducible vectors, confers mammalian G418 resistance, targets AAVS1 locus

    Depositor

    Gerold Schmitt-Ulms

    Article

    Wang et al Bio Protoc. 2020 May 5;10(9):e3615. doi:

    Insert

    G418 resistance
    

    Use

    CRISPR and Cre/Lox

    Expression

    Mammalian

    Promoter

    promoterless

    Available Since

    May 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSECC-sgTom

    Plasmid

    #138661

    Purpose

    Expresses a Tomato-targeting sgRNA, Cas9, and Cre-recombinase

    Depositor

    Reuben Shaw

    Article

    Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

    Insert

    sgTomato
    

    Use

    Lentiviral

    Promoter

    hU6

    Available Since

    March 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. AA027

    Plasmid

    #216007

    Purpose

    Fragmid fragment: (Cas protein) nearly PAM-less

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Has Service

    Cloning Grade DNA

    Insert

    Cas9-SpRY_v1.1 [Sp]
    

    Use

    CRISPR; Fragment

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. AA026

    Plasmid

    #216006

    Purpose

    Fragmid fragment: (Cas protein) NG PAM

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Has Service

    Cloning Grade DNA

    Insert

    Cas9-SpG_v1.1 [Sp]
    

    Use

    CRISPR; Fragment

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. LENTICRISPR-HK2_sgRNA1

    Plasmid

    #201596

    Purpose

    CRISPR/Cas9-mediated gene knock-out

    Depositor

    Vamsi Mootha, Alexis Jourdain

    Article

    Jourdain et al Mol Cell. 2021 May 6;81(9):1905-1919.e12

    Insert

    HK2 (HK2 Human)
    

    Use

    CRISPR and Lentiviral

    Available Since

    July 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLentiCRISPR-v1-sgGLS_6

    Plasmid

    #163460

    Purpose

    lentiviral vector expressing Cas9 and an sgRNA targeting GLS

    Depositor

    Jason Cantor

    Article

    Rossiter et al Cell Metab. 2021 Feb 23. pii: S1550-4131

    Insert

    sgRNA 6 targeting GLS (GLS Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Jan. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. lentiCRISPRv2-sgRNAs-del-ZNF91-mcherry

    Plasmid

    #202822

    Purpose

    Lentiviral vector modified to express two sgRNAs that delete exon 1 within the ZNF91 gene with EF-1apha for Cas9 and mcherry expression

    Depositor

    Matthew Anderson

    Article

    Nadler et al Commun Biol. 2023 Mar 30;6(1):347. doi:

    Insert

    two sgRNAs that delete exon 1 within the ZNF91 gene
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    U6 - two

    Available Since

    July 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pX330 Ctnnb1.2

    Plasmid

    #59912

    Purpose

    pX330 backbone expressing sgRNA targeting Ctnnb1 to edit mouse beta-Catenin. Expresses Cas9 from CBh promoter

    Depositor

    Tyler Jacks

    Article

    Xue et al Nature. 2014 Aug 6. doi: 10.1038/nature1

    Insert

    Ctnnb1 (Ctnnb1 Mouse)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    March 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. SPACE-ΔUGI (pRZ1836)

    Plasmid

    #140243

    Purpose

    CMV promoter expression plasmid for TadA*(V82G)-nCas9-pmCDA1(R187W)-P2A-EGFP

    Depositor

    Keith Joung

    Article

    Grunewald et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

    Insert

    SPACE-ΔUGI
    

    Expression

    Mammalian

    Mutation

    V82G in TadA*, D10A in Cas9, R187W in pmCDA1

    Promoter

    CMV

    Available Since

    June 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pWPT-/mEGFP-1T-IRES-mCherry

    Plasmid

    #190190

    Purpose

    Lentiviral expression vector with altered Kozak sequence to direct EGFP expression. mCherry expression is regulated by an IRES.

    Depositor

    Alessandro Quattrone

    Article

    Ambrosini et al Nucleic Acids Res. 2022 Oct 14;50(18):10

    Inserts

    mEGFP
    mCherry
    

    Use

    Lentiviral

    Mutation

    changed EGFP Kozak sequence inserting a C>T mo…

    Promoter

    EIF1-short

    Available Since

    Oct. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pODN-mNG-ECT2

    Plasmid

    #183835

    Purpose

    Repair template for the N-terminal tagging of Ect2 with mNeonGreen in human cells using CRISPR/Cas9.

    Depositor

    Alisa Piekny

    Article

    Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

    Insert

    ECT2 homology arms with mNeonGreen-linker (ECT2 Human)
    

    Use

    CRISPR; Donor template

    Tags

    mNeonGreen-linker

    Expression

    Mammalian

    Mutation

    Homology arms contain point mutations to remove t…

    Available Since

    Sept. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pLentiCriprV2-sgRNA-PER1-#1

    Plasmid

    #189987

    Purpose

    Lentiviral Cas9/sgRNA vector targeting C-terminus of hPER1, works with Addgene 189979-189982

    Depositor

    Achim Kramer

    Article

    Gabriel et al Proc Natl Acad Sci U S A. 2024 Aug 20;12

    Insert

    Period1 (PER1 Human)
    

    Use

    CRISPR and Lentiviral

    Available Since

    Feb. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. CGBE1-VRQR (pBM1192)

    Plasmid

    #140254

    Purpose

    CMV promoter expression plasmid for UNG(E.coli)-rAPOBEC1(R33A)-nCas9_VRQR-P2A-EGFP

    Depositor

    Keith Joung

    Article

    Kurt et al Nat Biotechnol. 2020 Jul 20. pii: 10.103

    Insert

    eUNG-BE4max(R33A)_VRQR-ΔUGI
    

    Expression

    Mammalian

    Mutation

    R33A in rAPOBEC1, VRQR mutations in SpCas9, D10A …

    Promoter

    CMV

    Available Since

    Aug. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. TS-PE2-C-WPRE

    Plasmid

    #176650

    Purpose

    Expresses C-terminus of PE2 in mammalian cells

    Depositor

    Hyongbum Kim

    Article

    Jang et al Nat Biomed Eng. 2021 Aug 26. pii: 10.103

    Inserts

    C-terminus of PE2
    Artificial SA
    WPRE
    bGH poly(A)
    

    Use

    AAV

    Tags

    SV40 NLS

    Expression

    Mammalian

    Promoter

    None

    Available Since

    Dec. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. TS-PE2-N

    Plasmid

    #176649

    Purpose

    Expresses N-terminus of PE2 in mammalian cells

    Depositor

    Hyongbum Kim

    Article

    Jang et al Nat Biomed Eng. 2021 Aug 26. pii: 10.103

    Inserts

    N-terminus of PE2
    Artificial SD
    

    Use

    AAV

    Tags

    SV40 NLS

    Expression

    Mammalian

    Promoter

    CMV and None

    Available Since

    Dec. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. SECURE BE4max(R33A) (pBM608)

    Plasmid

    #140251

    Purpose

    CMV promoter expression plasmid for rAPOBEC1(R33A)-nCas9-UGI-UGI-P2A-EGFP

    Depositor

    Keith Joung

    Article

    Kurt et al Nat Biotechnol. 2020 Jul 20. pii: 10.103

    Insert

    BE4max(R33A)
    

    Expression

    Mammalian

    Mutation

    R33A in rAPOBEC1, D10A in Cas9

    Promoter

    CMV

    Available Since

    Aug. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pLenti-sgRELA

    Plasmid

    #83944

    Purpose

    Lentiviral vector expressing an sgRNA targeting RELA NOTE: This plasmid does NOT contain Cas9.

    Depositor

    David Sabatini

    Article

    Park et al Nat Genet. 2016 Dec 19. doi: 10.1038/ng.

    Insert

    sgRELA
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. AA007

    Plasmid

    #216002

    Purpose

    Fragmid fragment: (Cas protein) canonical Cas9; NGG PAM

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Has Service

    Cloning Grade DNA

    Insert

    Cas9_v1.1 [Sp]
    

    Use

    CRISPR; Fragment

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pLenti-Hygro-sgSIK3

    Plasmid

    #138699

    Purpose

    Expresses a human SIK3-targeting sgRNA and Cas9

    Depositor

    Reuben Shaw

    Article

    Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

    Insert

    sgSIK3 human (SIK3 Human)
    

    Use

    Lentiviral

    Promoter

    hU6

    Available Since

    Feb. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. LentiCRISPRCreb5gRNA

    Plasmid

    #195021

    Purpose

    Sequence specific sgRNA that guide Cas9 to the genomic region encoding the bovine Creb5 DNA binding domain

    Depositor

    Andrew Lassar, Cheng-Hai Zhang

    Article

    Zhang et al Commun Biol. 2021 Mar 12;4(1):332. doi:

    Insert

    Creb5 gRNA (CREB5 Bovine)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Feb. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pLenti-Puro-SIK1

    Plasmid

    #138695

    Purpose

    Expresses a human SIK1-targeting sgRNA and Cas9

    Depositor

    Reuben Shaw

    Article

    Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

    Insert

    sgSIK1 human (SIK1 Human)
    

    Use

    Lentiviral

    Promoter

    hU6

    Available Since

    Feb. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pLenti-Blast-sgSIK2

    Plasmid

    #138708

    Purpose

    Expresses a human SIK2-targeting sgRNA and Cas9

    Depositor

    Reuben Shaw

    Article

    Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

    Insert

    sgSIK2 human (SIK2 Human)
    

    Use

    Lentiviral

    Promoter

    hU6

    Available Since

    Feb. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pJL1-mRFP1 gRNA

    Plasmid

    #117052

    Purpose

    In vitro expression of anti-mRFP1 Cas9 sgRNA from the T7 promoter

    Depositor

    Michael Jewett

    Article

    Stark et al ACS Synth Biol. 2019 May 7. doi: 10.1021

    Insert

    anti-mRFP1 SpCas9 gRNA
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. LentiCRISPR-sgSHOC2-1

    Plasmid

    #86128

    Purpose

    Lentiviral vector expressing Cas9 and an sgRNA targeting SHOC2

    Depositor

    David Sabatini

    Article

    Wang et al Cell. 2017 Feb 1. pii: S0092-8674(17)300

    Insert

    sgRNA 1 targeting SHOC2 (SHOC2 )
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Feb. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. LENTICRISPR-HNRNPD_sgRNA1

    Plasmid

    #201598

    Purpose

    CRISPR/Cas9-mediated gene knock-out

    Depositor

    Vamsi Mootha, Alexis Jourdain

    Article

    Jourdain et al Mol Cell. 2021 May 6;81(9):1905-1919.e12

    Insert

    HNRNPD (HNRNPD Human)
    

    Use

    CRISPR and Lentiviral

    Available Since

    June 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. LentiCRISPR-sgSHOC2-2

    Plasmid

    #86129

    Purpose

    Lentiviral vector expressing Cas9 and an sgRNA targeting SHOC2

    Depositor

    David Sabatini

    Article

    Wang et al Cell. 2017 Feb 1. pii: S0092-8674(17)300

    Insert

    sgRNA 2 targeting SHOC2 (SHOC2 )
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    May 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pSECC-sgLKB1-A

    Plasmid

    #138663

    Purpose

    Expresses a mouse LKB1-targeting sgRNA, Cas9, and Cre-recombinase

    Depositor

    Reuben Shaw

    Article

    Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

    Insert

    sgLKB1 mouse (Stk11 Mouse)
    

    Use

    Lentiviral

    Promoter

    hU6

    Available Since

    March 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. px335-EF-Slc2a3-L

    Plasmid

    #122304

    Purpose

    Expresses sgRNA targeting mouse Slc2a3 and Cas9 nickase in mammalian cells

    Depositor

    Hiroshi Ochiai

    Article

    Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

    Insert

    sgRNA for mouse Slc2a3 (Slc2a3 Synthetic)
    

    Expression

    Mammalian

    Available Since

    June 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. LENTICRISPR-HK2_sgRNA2

    Plasmid

    #201597

    Purpose

    CRISPR/Cas9-mediated gene knock-out

    Depositor

    Vamsi Mootha, Alexis Jourdain

    Article

    Jourdain et al Mol Cell. 2021 May 6;81(9):1905-1919.e12

    Insert

    HK2 (HK2 Human)
    

    Use

    CRISPR and Lentiviral

    Available Since

    June 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pLenti-Puro-SIK2

    Plasmid

    #138696

    Purpose

    Expresses a human SIK2-targeting sgRNA and Cas9

    Depositor

    Reuben Shaw

    Article

    Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

    Insert

    sgSIK2 human (SIK2 Human)
    

    Use

    Lentiviral

    Promoter

    hU6

    Available Since

    Feb. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge