We narrowed to 81,735 results for: TRI
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pSOMA-NES
Plasmid#118939PurposeUsed to express the SOMA FRET sensor with a nuclear exclusion signal in Arabidopsis thalianaDepositorInsertSOMA Sensor for Arabidopsis MAPK activity with Nuclear Exclusion Signal
ExpressionPlantAvailable SinceFeb. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFETCh_CEBPB
Plasmid#64055PurposeHomology Arms and 3X Flag with P2A Neo for 3' tagging of human CEBPBDepositorInsertCEBPB Homology Arms (CEBPB Human)
Tags3X Flag P2A NeoAvailable SinceJuly 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
AAV-PGK-eGFP
Plasmid#193023PurposeAAV vector plasmid expressing enhanced green fluorescent protein (eGFP) under the mouse phosphoglycerate kinase (PGK) promoterDepositorInserteGFP
UseAAVExpressionMammalianPromoterPGKAvailable SinceFeb. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAR-B1(1986.1)
Plasmid#78503PurposeTrans plasmid for production of recombinant AAV-B1 vectors. Plasmid encodes AAV2 Rep and AAV-B1 capsidDepositorInsertsAAV2 Rep
AAV-B1 capsid
UseAAVExpressionMammalianAvailable SinceJune 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pC011 LwCas13a locus with targeting spacer
Plasmid#91903PurposeLwCas13a locus into pACYC184 with targeting spacerDepositorInsertLwCas13a with targeting spacer
UseCRISPRExpressionBacterialAvailable SinceOct. 16, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
PX458_AHR_1
Plasmid#101076PurposeEncodes gRNA for 3' target of human AHRDepositorInsertgRNA against AHR (AHR Human)
UseCRISPRAvailable SinceOct. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-CBX1mut-PA-mCherry
Plasmid#138251PurposeTriple-mutant CBX1 chromodomain (residues 20-73) tagged with photoactivatable mCherry (PAmCherry). Mutations V3E/K6E/D40S correspond to positions V22/K25/D59 in full-length CBX1.DepositorInsertCBX1mut-PAmCherry
Tags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianPromoterCMVAvailable SinceJuly 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Syn-pAceR-Kv2.1PR
Plasmid#195525PurposeRed fluorescent, positive response-polarity voltage indicator under the control of synapsin promoter; soma-targetedDepositorInsertpAceR-Kv2.1 proximal restriction sequence
UseAAVExpressionMammalianMutationVARNAM 78E, 81D, 92N; SI linkerPromoterSynapsinAvailable SinceJan. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJT396 PB ITR and Cp36 attD pEF Puro-2a-tagBFP2 donor
Plasmid#184952PurposeDonor for both PiggyBac and Cp36 RecombinaseDepositorInsertPuroR, P2A, mTagBFP2
ExpressionMammalianAvailable SinceOct. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCD5-D/bovine/Nebraska/9-5/2012-HEFs57aED-GCN4-sfGFP-ST
Plasmid#175020PurposeExpresses influenza D virus trimeric hemagglutinin esterase fusion protein fused to a sfGFPDepositorInsertD/bovine/Nebraska/9-5/2012
TagsGCN4-TEV-sfGFP-TwinStrepExpressionMammalianMutations57a esterase knock out mutantPromoterCMVAvailable SinceJan. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
bArr2-ALFA
Plasmid#201498PurposeExpression of the beta-arrestin2 containing a N-terminal ALFA tag.DepositorAvailable SinceAug. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pKMD111e - intein-Cas9(S219-G521R)
Plasmid#64192PurposeExpresses intein-Cas9(S219-G521R) in mammalian cellsDepositorInsertmammalian codon-optimized S. pyogenes intein-Cas9(S219-G521R) - NLS - 3x FLAG
UseCRISPRTagsNLS - 3x FLAG (C terminal on insert)ExpressionMammalianPromoterCMVAvailable SinceJuly 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pC009 LshCas13a locus with targeting spacer
Plasmid#91900PurposeLshCas13a locus into pACYC184 with targeting spacerDepositorInsertLshCas13a locus with targeting spacer
ExpressionBacterialAvailable SinceOct. 16, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLKO-puro-tetON-shGFP
Plasmid#110939PurposeTet-inducible shRNA targeting GFPDepositorInsertshGFP
UseLentiviral and RNAiPromoterH1/TOAvailable SinceAug. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMx/ATP9B-HA
Plasmid#209231PurposeMammalian expression of ATP9BDepositorAvailable SinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
PX458_KDM1A_1
Plasmid#86297PurposeEncodes gRNA for 3' target of human KDM1ADepositorInsertgRNA against KDM1A (KDM1A Human)
UseCRISPRAvailable SinceFeb. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLV-GG-hUBC-EBFP2
Plasmid#212314PurposeLentiviral backbone with EBFP2 reporterDepositorTypeEmpty backboneUseCRISPR and LentiviralExpressionMammalianAvailable SinceJune 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
PX458_KDM1A_2
Plasmid#86298PurposeEncodes gRNA for 3' target of human KDM1ADepositorInsertgRNA against KDM1A (KDM1A Human)
UseCRISPRAvailable SinceJan. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-FDIO-COX4-dAPEX2
Plasmid#117178PurposePlasmid name in publication: pAAV-FDIO-Matrix-dAPEX2. Peroxidase reporter for multiplexed electron microscopy labelingDepositorInsertCOX4-dAPEX2
UseAAV; Flp/frtExpressionMammalianMutationW41F and A134P on soybean APXPromoterEF1aAvailable SinceMarch 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
EC71171
Plasmid#154069PurposeLevel 0 Golden Gate vector, CRE with U5 intron at 254bp, in pICH41308 backboneDepositorInsertCRE_U5intron
UseSynthetic BiologyMutationAll BsaI, Esp3I and BPiI restriction sites were r…Available SinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFBD-Akt1(E17K)
Plasmid#86563PurposeExpresses human full-length Akt1(E17K) mutantDepositorAvailable SinceMarch 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCD564
Plasmid#153026PurposedxCas9(3.7), MCP-SoxS(R93A/S101A)DepositorInsertdxCas9(3.7), MCP-SoxS(R93A/S101A)
UseCRISPRExpressionBacterialMutationSoxS has R93A and S101A mutationsAvailable SinceAug. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
UAS-u(S)Cas9
Plasmid#127383PurposeExpresses Cas9 at high levelDepositorInsertuORF(S)-Cas9
UseCRISPRExpressionInsectPromoterUASTAvailable SinceJune 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLX304 GDF11-V5 blast
Plasmid#83098PurposeLentiviral vector for constitutive expression of human GDF11 with C-terminal V5 tagDepositorAvailable SinceFeb. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMIR-Dsred-IRES-His-Halo-Tev-Keap C288S
Plasmid#62459Purposeexpresses his-tagged C288S human Keap1 proteinDepositorInsertKeap1 (KEAP1 Human)
TagsHis-Halo-TevExpressionMammalianMutationChanged Cysteine 288 of Keap1 to serinePromoterCMVAvailable SinceMarch 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
Man2a1-myc
Plasmid#163648PurposeExpresses myc tagged Man2a1 in mammalian cellsDepositorInsertmannosidase alpha class 2A member 1 (MAN2A1 Human)
TagsmycExpressionMammalianMutationSilent mutation was made to introduce a XbaI rest…Available SinceNov. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pK1E-RNAP
Plasmid#48144PurposeShuttle vector E. coli/B.meg.; encoding the RNA polymerase of the phage K1E under control of the xylose inducible promoter PxylADepositorInsertrna polymerase K1E
UseSynthetic BiologyExpressionBacterialAvailable SinceOct. 21, 2013AvailabilityAcademic Institutions and Nonprofits only -
pEIAV-DIO-TLoop-CHR2YFP
Plasmid#66798Purposeexpression of channelrhodopsin-2-YFP, a light-gated cation channel that can depolarize cells via sodium influx when blue light changes its conformation, using a DIO-based TLoop EIAV vectorDepositorInsertFloxed Chr2-YFP
UseLentiviralTagscitrineExpressionMammalianMutationH134RPromoterCMVTetO7 PromoterAvailable SinceJune 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
DEL-pHG165a
Plasmid#90064PurposeLLhG coding region on pHG165a interrupted with a single base-pair deletion, 'no repressor' control plasmidDepositorInsertDEL
ExpressionBacterialPromoterIqAvailable SinceJune 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET15b-sfGO-Matryoshka-LE-LP
Plasmid#100018PurposeBacterial expression of fluorescent protein casette - cpsfGFP with nested LSSmOrange and LE N-term and LP C-term amino acid linkersDepositorInsertcpsfGFP-LSSmOrange
Tags6x HIS tagExpressionBacterialMutationLeu and Glu N-term linker and Leu and Pro C-term …PromoterT7Available SinceOct. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
LacI-11/109-pHG165c
Plasmid#90059PurposeDimeric LacI (truncated last 11 amino acids) polymorphism with T at position 109DepositorInsertLacI-11/109
ExpressionBacterialMutation109PromoterIqAvailable SinceJune 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pX330 _CRISPIE donor B10
Plasmid#172851PurposeDRS-2 sgRNA expression under a U6 promotor and CRISPIE donor B10 (Zhong et al, eLife 2021), mEGFP translational phase (2-stop), excised by DRS-1 or DRS-2 sgRNA (with SpCas9).DepositorInsertDRS-2 sgRNA and CRISPIE designer exon (phase 2-stop) encoding mEGFP
UseCRISPRExpressionMammalianAvailable SinceAug. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFETCh_CEBPA
Plasmid#86293PurposeDonor vector for 3' FLAG tag of human CEBPADepositorAvailable SinceAug. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pENTR4 TNRC6A
Plasmid#20021DepositorInsertTrinucleotide repeat containing 6A (TNRC6A Human)
UseEntry vectorAvailable SinceJan. 12, 2009AvailabilityAcademic Institutions and Nonprofits only -
PX458_AHR_2
Plasmid#101077PurposeEncodes gRNA for 3' target of human AHRDepositorInsertgRNA against AHR (AHR Human)
UseCRISPRAvailable SinceOct. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
EGFP-IC2-N237
Plasmid#51410Purposeexpression of GFP-tagged N-terminal AA 1-237 of dynein intermediate chain IC2C in mammalian cellsDepositorInsertdynein intermediate chain IC2C (Dync1i2 Mouse)
TagsEGFPExpressionMammalianMutationAA 1-237PromoterCMVAvailable SinceFeb. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
pRS406-PHO5-GFP-OSBP PH domain
Plasmid#58840PurposeExpresses GFP-Tagged OSBP PH domain in yeastDepositorAvailable SinceJan. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pX330_Actb sgRNA / hSpCas9
Plasmid#172832PurposeMammalian expression of a sgRNA targeting the intron 1of Actb (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.DepositorInsertsgRNA targeting intron 1 of Actb under the U6 promotor and hSpCas9 under the CAG promotor
UseCRISPRExpressionMammalianAvailable SinceAug. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRSET a MatryoshCaMP6s
Plasmid#100019PurposeBacterial expression of fluorescent reporter for calcium signaling, based off of GCaMP6s. Contains a stable reference Large Stokes Shift (LSS) mOrange nested within the reporting cpEGFP.DepositorInsertMatryoshCaMP6s
Tags6x HIS tag and Xpress_EK tagExpressionBacterialPromoterT7Available SinceOct. 3, 2017AvailabilityAcademic Institutions and Nonprofits only
