We narrowed to 23,940 results for: nes

1. S3_002_026_096

   Plasmid

   #134403

   Purpose

   pYTK096-pRPL18B_NLS_FdeR-N_tPGK1-pGPM1-fdeO_mcherry_tTDH1

   Depositor

   Mattheos Koffas

   Article

   Wang et al ACS Synth Biol. 2019 Sep 20;8(9):2121-21

   Inserts

   mCherry
   NLS_FdeR-N
   

   Use

   Tags

   None and none

   Expression

   Yeast

   Mutation

   None and none

   Promoter

   pGPM1 and pRPL18B

   Available since

   Dec. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pAct:MS2-p65-HSF

   Plasmid

   #78902

   Purpose

   Expresses MS2-p65-HSF under actin promoter for SAM CRISPRa in Drosophila cells

   Depositor

   Norbert Perrimon

   Article

   Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

   Insert

   MS2-p65-HSF
   

   Use

   CRISPR

   Tags

   Expression

   Insect

   Mutation

   Promoter

   pActin (Drosophila)

   Available since

   Sept. 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. SB53

   Plasmid

   #21488

   Depositor

   Shoumo Bhattacharya

   Article

   Bhattacharya et al Genes Dev. 1999 Jan 1. 13(1):64-75.

   Insert

   Cited2 (CITED2 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   aa1-65 deleted

   Promoter

   Available since

   Aug. 12, 2009

   Availability

   Academic Institutions and Nonprofits only

4. pOME0005R

   Plasmid

   #178374

   Purpose

   Expresses KSHV ORF K2

   Depositor

   Dirk Dittmer

   Article

   Alzhanova et al PLoS Pathog. 2021 Jan 7;17(1):e1009033.

   Insert

   KSHV ORF K2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pOME0006L

   Plasmid

   #178376

   Purpose

   Expresses KSHV K2 ORF tagged

   Depositor

   Dirk Dittmer

   Article

   Alzhanova et al PLoS Pathog. 2021 Jan 7;17(1):e1009033.

   Insert

   KSHV ORF K2
   

   Use

   Lentiviral

   Tags

   3xFLAG

   Expression

   Mutation

   Promoter

   Available since

   Jan. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBS/KS-mId4sp

   Plasmid

   #16046

   Depositor

   Robert Benezra

   Article

   Jen et al Dev Dyn. 1996 Nov . 207(3):235-52.

   Insert

   Id4 (Id4 Mouse)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   cDNA nucleotides 665-945

   Promoter

   Available since

   Nov. 2, 2007

   Availability

   Academic Institutions and Nonprofits only

7. pPV230.13

   Plasmid

   #14902

   Depositor

   James Lok

   Article

   Li et al Int J Parasitol. 2006 May 31. 36(6):671-

   Insert

   Strongyloides stercoralis transitional endoplasmic reticulum ATPase promoter region
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Ss era-1 promoter region as a HindIII-PstI fragme…

   Promoter

   Available since

   July 18, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. GFP-mROCK1-delta5

   Plasmid

   #101293

   Purpose

   Mammalian expression of Rock1-GFP for the study of localisation signaling.

   Depositor

   Alpha Yap

   Article

   Priya et al Nat Cell Biol. 2015 Oct;17(10):1282-93.

   Insert

   Rock1 (Rock1 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   375-727 aa

   Promoter

   CMV

   Available since

   Oct. 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. p3.2mar-GFP

   Plasmid

   #63694

   Purpose

   3.2 kb marine armor plate enhancer driving GFP reporter

   Depositor

   David Kingsley

   Article

   O'Brown et al Elife. 2015 Jan 28;4:e05290. doi: 10.755

   Insert

   3.2 kb marine stickleback armor plate enhancer
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   hsp70

   Available since

   April 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCEP4-SERTG56A -myc

    Plasmid

    #107459

    Purpose

    Mammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation G56A conferring wildtype phenotype

    Depositor

    Erik Procko

    Article

    Young et al bioRxiv 2021.04.19.440442

    Insert

    Serotonin Transporter (SERT) (SLC6A4 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    G56A

    Promoter

    CMV

    Available since

    March 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. p5E-pax3a-5.4k

    Plasmid

    #164966

    Purpose

    zebrafish pax3a -5.4k promoter

    Depositor

    GuangJun Zhang

    Article

    Silic et al Genetics. 2020 Aug;215(4):1067-1084. doi

    Insert

    pax3a (pax3a Zebrafish)
    

    Use

    Gateway 5 end entry vector

    Tags

    Expression

    Mutation

    promoter -5.4k

    Promoter

    Available since

    March 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pDIRECT_21J

    Plasmid

    #91132

    Purpose

    Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:TALEN, Plant Selection: 2x35S:hpt II

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    Engineering Reagent: 35S:TALEN
    

    Use

    TALEN

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    July 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pALPS_3'LTR GFP@gag start SFFV tat

    Plasmid

    #101338

    Purpose

    LTR drives GFP and internal SFFV promoter drives codon optimized tat

    Depositor

    Jeremy Luban

    Article

    McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

    Insert

    GFP, tat
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pV1386

    Plasmid

    #111445

    Purpose

    Solo vector pV1382 + sgScADE2

    Depositor

    Gerald Fink

    Article

    Vyas et al mSphere. 2018 Apr 25;3(2). pii: 3/2/e001

    Insert

    CaCas9/sgScADE2/stop-codon repair
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Dec. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCFD3-MS2tail-SAM

    Plasmid

    #78905

    Purpose

    Vector for expressing a single sgRNA with SAM MS2 tail, under U6:3 promoter

    Depositor

    Norbert Perrimon

    Article

    Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Aug. 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. BII-ChBtW-PLAGL2

    Plasmid

    #133369

    Purpose

    Piggybac vector for constitutive expression

    Depositor

    Blair Madison

    Article

    Fischer et al Am J Physiol Gastrointest Liver Physiol.

    Insert

    PLAGL2 (PLAGL2 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    CMV enhancer and hEf1a (CpG free)

    Available since

    Feb. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. MS2_adRNA

    Plasmid

    #124624

    Purpose

    Expresses MS2_adRNA targeting the RAB7A transcript along with GFP

    Depositor

    Prashant Mali

    Article

    Katrekar et al Nat Methods. 2019 Mar;16(3):239-242. doi

    Insert

    MS2_adRNA_RAB7A
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    May 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pG3K-U6EC1

    Plasmid

    #134757

    Purpose

    pGreen3 CRISPR/Cas9

    Depositor

    Qi-Jun Chen

    Article

    Zhang et al Plant Physiol. 2019 Sep 26. pii: pp.19.0

    Insert

    CRISPR/Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    gRNA-U6, zCas9-EC1

    Available since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pG3B-U6EC1

    Plasmid

    #134756

    Purpose

    pGreen3 CRISPR/Cas9

    Depositor

    Qi-Jun Chen

    Article

    Zhang et al Plant Physiol. 2019 Sep 26. pii: pp.19.0

    Insert

    CRISPR/Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    gRNA-U6, zCas9-EC1

    Available since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pG3H-U3Ub

    Plasmid

    #134749

    Purpose

    pGreen3 CRISPR/Cas9

    Depositor

    Qi-Jun Chen

    Article

    Zhang et al Plant Physiol. 2019 Sep 26. pii: pp.19.0

    Insert

    CRISPR/Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    gRNA- OsU3, zCas9-Ubi1

    Available since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pYNL-C1_H2B

    Plasmid

    #65702

    Purpose

    Expresses YNL-H2B in mammalian cells

    Depositor

    Yasushi Okada

    Article

    Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

    Insert

    histone H2B
    

    Use

    Luciferase

    Tags

    YNL

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. Tol2-lyzC-microRNA 722-Dendra2

    Plasmid

    #97130

    Purpose

    overexpression of microRNA and Dendra2 in Zebrafish

    Depositor

    Qing Deng

    Article

    Hsu et al Dis Model Mech. 2017 Sep 27. pii: dmm.03

    Insert

    microRNA 722 Dendra2 (mir722 Zebrafish)
    

    Use

    Zebrafish

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pSR-puro-Sec5

    Plasmid

    #31117

    Depositor

    Christopher Counter

    Article

    Issaq et al Mol Cancer Res. 2010 Feb . 8(2):223-31.

    Insert

    Sec5 shRNA (EXOC2 Human)
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 3, 2011

    Availability

    Academic Institutions and Nonprofits only

24. pMOD_A0301

    Plasmid

    #91005

    Purpose

    Module A, Promoter: 35S, Gene: AtCas9_H840A nickase, Terminator: HSP

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    AtCas9_H840A nickase
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    H840A

    Promoter

    35S

    Available since

    July 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. mCerulean3-PTK2-N-10

    Plasmid

    #55442

    Purpose

    Localization: FAK/Focal Adhesions, Excitation: 433, Emission: 475

    Depositor

    Michael Davidson

    Article

    Markwardt et al PLoS One. 2011 . 6(3):e17896.

    Insert

    PTK2 (PTK2 Human)
    

    Use

    Tags

    mCerulean3

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

26. pcDNA FLAG TORC1 (1-630)

    Plasmid

    #22974

    Depositor

    Marc Montminy

    Article

    Conkright et al Mol Cell. 2003 Aug . 12(2):413-23.

    Insert

    TORC1 (1-630) (CRTC1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Amino acids 1-630 rather than 1-634.

    Promoter

    Available since

    Jan. 22, 2010

    Availability

    Academic Institutions and Nonprofits only

27. pCFJ2214 - Intron GG1 - 900 bp

    Plasmid

    #159880

    Purpose

    Golden Gate compatible intron with PATCs for reduced germline silencing

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    Intron GG1 - 900 bp
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Not applicable

    Promoter

    Available since

    March 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. 4xPylT(AGGA)Ev2 PylS mCherry-P2A-eGFP(AGGA)

    Plasmid

    #174529

    Purpose

    Dual-fluorescence reporter for quantifying PylT(AGGA)Ev2 decoding efficiency

    Depositor

    Yu-Hsuan Tsai

    Article

    Mills et al Cell Rep Methods. 2021 Sep 16;1(6):10007

    Inserts

    PylS
    PylT
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Oct. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. BPK1160

    Plasmid

    #179298

    Purpose

    Expresses dCas9 fused to P65

    Depositor

    Keith Joung

    Article

    Tak et al Nat Methods. 2021 Sep;18(9):1075-1081. d

    Insert

    dCas9-p65
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    D10A, H840A (catalytically inactive Cas9)

    Promoter

    CAG

    Available since

    Feb. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. XE28 XBC 40

    Plasmid

    #16842

    Depositor

    Randall Moon

    Article

    Yost et al Genes Dev. 1996 Jun 15. 10(12):1443-54.

    Insert

    beta-catenin-myc (ctnnb1.L Frog)
    

    Use

    Xenopus expression

    Tags

    6x myc

    Expression

    Mutation

    Promoter

    Available since

    May 22, 2009

    Availability

    Academic Institutions and Nonprofits only

31. Ad-shMKP-3

    Plasmid

    #131530

    Purpose

    Silence the MKP-3 expression

    Depositor

    Haiyan Xu

    Article

    Jiao et al PLoS One. 2012;7(7):e41168. doi: 10.1371

    Insert

    Short hairpin mitogen-activated protein kinase phosphatase 3 (Dusp6 Mouse)
    

    Use

    Adenoviral

    Tags

    No

    Expression

    Mutation

    Promoter

    Available since

    Nov. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. Desmocollin2_tail-GST

    Plasmid

    #36992

    Depositor

    Kathleen Green

    Article

    Nekrasova et al J Cell Biol. 2011 Dec 26;195(7):1185-203

    Insert

    Dsc2 (DSC2 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Intracellular domain of Dsc2 (nt 2,603–3,158)

    Promoter

    Available since

    July 31, 2012

    Availability

    Academic Institutions and Nonprofits only

33. px330 EQR

    Plasmid

    #101733

    Purpose

    Expresses a sgRNA and a Cas9 EQR variant that recognizes "NGAG" PAM motifs

    Depositor

    Andrew Holland

    Article

    Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

    Insert

    SpCas9 EQR
    

    Use

    Tags

    3xFLAG-NLS and NLS

    Expression

    Mammalian

    Mutation

    EQR (D1135E, R1335Q, and T1337R mutations in Cas9)

    Promoter

    Available since

    Oct. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pCMB-TMEg

    Plasmid

    #61209

    Purpose

    Fluorescent reporter for plasma membrane and trunk peri-arbuscular membrane MtBCPsp-GFP-BCP expressed under MtBCP1 promoter

    Depositor

    Maria Harrison

    Article

    Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

    Insert

    MtBCPsp-GFP-BCP
    

    Use

    Tags

    EGFP

    Expression

    Plant

    Mutation

    Promoter

    MtBCP1

    Available since

    April 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pBSHSP:FLP;CMV:tdTomato-SceI

    Plasmid

    #30523

    Depositor

    Gerhart Ryffel

    Article

    Roose et al Transgenic Res. 2009 Aug . 18(4):595-605

    Insert

    FLP recombinase tdTomato
    

    Use

    Flp/frt, heat shock inducible, i-scei megabase ve…

    Tags

    Expression

    Mutation

    R415H

    Promoter

    Available since

    Oct. 17, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pcDNA3.1(+) EPHB4 Antisense

    Plasmid

    #60640

    Purpose

    Negative Control for the pcDNA3.1(+) EPHB4 Sense plasmid

    Depositor

    Jeremy Wilusz

    Article

    Liang et al Genes Dev. 2014 Oct 3. pii: gad.251926.1

    Insert

    Ephrin type B receptor 4 (EPHB4 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pH6HTC_IGF2BP2b-Halo

    Plasmid

    #175318

    Purpose

    Production of HaloTag fusion protein

    Depositor

    Claudia Kutter

    Article

    Atanasoai et al bioRxiv 2021.11.08.467743

    Insert

    IGF2BP2 (IGF2BP2 Human)
    

    Use

    Cell free expression

    Tags

    HaloTag-6xHis

    Expression

    Bacterial

    Mutation

    Isoform B

    Promoter

    Available since

    Oct. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. RNAPII_CTD38-52

    Plasmid

    #98685

    Purpose

    Expresses 6xHis-tagged C-terminal domain of RNA polymerase II

    Depositor

    Nicolas Fawzi

    Article

    Janke et al Biochemistry. 2017 Oct 11. doi: 10.1021/

    Insert

    RNA polymerase II C-terminal domain (POLR2A Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    heptads 38-52, residues 1853-1970

    Promoter

    Available since

    Nov. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pGFP-Ub-VPS13D_dN

    Plasmid

    #176465

    Purpose

    express VPS13D lacking N-terminal 117 aa in mammalian cells

    Depositor

    Richard Youle

    Article

    Baldwin et al J Cell Biol. 2021 May 3;220(5). pii: 212

    Insert

    VPS13D (VPS13D Human)
    

    Use

    Tags

    GFP-Ub

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pRUSH

    Plasmid

    #54479

    Purpose

    Mouse genomic ROSA26 targeting vector, expresses EGFP, U6promoter to drive short RNAs, pgkneo, cassette flanked by loxP sites

    Depositor

    Laurie Jackson-Grusby

    Article

    Brown et al Genesis. 2014 Jan;52(1):39-48. doi: 10.1

    Type

    Empty backbone

    Use

    Cre/Lox, Mouse Targeting, and RNAi

    Tags

    SA-EGFP-polyA, U6 promoter, loxP, and pgk-neo

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Aug. 18, 2014

    Availability

    Academic Institutions and Nonprofits only