We narrowed to 2,905 results for: BFP
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Plasmid#117268PurposeRetroviral overexpression of Lrrc40 - truncated version with seemingly same DE genes in Th2 as MSCV-Lrrc40-IRES-BFPDepositorInsertLrrc40 (Lrrc40 Mouse)
UseRetroviralExpressionMammalianMutationThe insert is truncated - deletion of bp 334-407 …Available SinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6(gDmap1 v4)-PGK-Puro-BFP
Plasmid#117143PurposeExpress gRNA vs Dmap1DepositorInsertgDmap1 v4 (Dmap1 Synthetic)
UseCRISPR and LentiviralMutationDeleted BbsI site within WPRE, modified BFPPromoterhuman U6 promoter, mouse PGK promoterAvailable SinceApril 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6(gDmap1 v5)-PGK-Puro-BFP
Plasmid#117144PurposeExpress gRNA vs Dmap1DepositorInsertgDmap1 v5 (Dmap1 Synthetic)
UseCRISPR and LentiviralMutationDeleted BbsI site within WPRE, modified BFPPromoterhuman U6 promoter, mouse PGK promoterAvailable SinceApril 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6(gDmap1 v1)-PGK-Puro-BFP
Plasmid#117140PurposeExpress gRNA vs Dmap1DepositorInsertgDmap1 v1 (Dmap1 Synthetic)
UseCRISPR and LentiviralMutationDeleted BbsI site within WPRE, modified BFPPromoterhuman U6 promoter, mouse PGK promoterAvailable SinceApril 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6(gDmap1 v2)-PGK-Puro-BFP
Plasmid#117141PurposeExpress gRNA vs Dmap1DepositorInsertgDmap1 v2 (Dmap1 Synthetic)
UseCRISPR and LentiviralMutationDeleted BbsI site within WPRE, modified BFPPromoterhuman U6 promoter, mouse PGK promoterAvailable SinceApril 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEJS592-pCSDest2-AcrIIC4Hpa-BFPv2-IRES
Plasmid#113438PurposeExpresses type II-C anti-CRISPR protein from H. parainfluenzae and a BFP from IRES in mammalian cellsDepositorInsertsType II-C anti-CRISPR
mTagBFP2
ExpressionMammalianPromoterCMVAvailable SinceDec. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEJS593-pCSDest2-AcrIIC5Smu-BFPv2-IRES
Plasmid#113439PurposeExpresses type II-C anti-CRISPR protein from S. muelleri and a BFP from IRES in mammalian cellsDepositorInsertsType II-C anti-CRISPR
mTagBFP2
ExpressionMammalianPromoterCMVAvailable SinceDec. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV-AID-PcrA M6-UGI-T2A-mTagBFP2
Plasmid#221225PurposeExpress HACE Editor as a fusion of AID cytidine deaminase and an optimized PcrA M6 helicase with mTagBFP2 reporterDepositorInsertAID-PcrA M6-UGI-T2A-mTagBFP2
ExpressionMammalianAvailable SinceAug. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-AID-BLM-UGI-T2A-mTagBFP2
Plasmid#221222PurposeExpress HACE Editor as a fusion of AID cytidine deaminase and BLM helicase with mTagBFP2 reporterDepositorInsertAID-BLM-UGI-T2A-mTagBFP2
ExpressionMammalianAvailable SinceJune 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pU6-mir451 EF1Alpha-puro-T2A-BFP
Plasmid#164792PurposeExpress miR-451 under the U6 promoter in mammalian cellsDepositorInsertsUseLentiviralExpressionMammalianPromoterEF1Alpha and U6Available SinceMarch 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV-AID-Ns3h-UGI-T2A-mTagBFP2
Plasmid#221223PurposeExpress HACE Editor as a fusion of AID cytidine deaminase and Ns3h helicase with mTagBFP2 reporterDepositorInsertAID-Ns3h-UGI-T2A-mTagBFP2
ExpressionMammalianAvailable SinceFeb. 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
CROPseq-multi-v2-mTagBFP2-NLS-BsmBI_entry (pRW1506)
Plasmid#225754PurposeEntry vector to clone sgRNA(s) into the CROPseq-multi-v2 construct with nuclear-localized mTagBFP2-NLS; v2 is compatible with T7 in vitro transcription detectionDepositorTypeEmpty backboneUseCRISPR and LentiviralExpressionMammalianAvailable SinceSept. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
Ef1a-pspCas13b-NES-3xFLAG-T2A-BFP
Plasmid#173029PurposeFor expression of cytoplasmic pspCas13b protein tagged with 3xHA-T2A-BFP for gene silencing in mamallian cells.DepositorInsertpspCas13b
UseCRISPR and LentiviralTags3xFLAG, BFP, HIV NES, and T2AExpressionMammalianPromoterEf1aAvailable SinceSept. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pU6-sgRNA EF1Alpha-puro-T2A-BFP
Plasmid#60955PurposeParental vector for the CRISPRi/a libraries. Expresses an sgRNA from the U6 promoter and a puromycin resistance cassette and BFP from the EF1Alpha promoterDepositorInsertssgGFP-NT2
puro-T2A-BFP
UseCRISPR and LentiviralExpressionMammalianAvailable SinceJan. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pU6-sgRosa26-1_CBh-Cas9-T2A-BFP
Plasmid#64216PurposeExpression vector for a sgRNA against the mouse Rosa26 locus and Cas9 linked to BFP via a T2A peptideDepositorInsertsCas9
sgRNA targeting ROSA26-1
UseCRISPRTags3xFLAG, NLS, and T2A-BFPExpressionMammalianPromoterCBh and U6Available SinceMay 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLenti-Tet-coBxb1-2A-BFP_IRES-iCasp9-2A-Blast_rtTA3
Plasmid#171588PurposeLentivector encoding a DNA recombinase landing pad with Bxb1 integrase and iCasp9 negative selection behind a Tet-inducible promoterDepositorInsertsBxb1 integrase
iCasp9
BFP
rtTA3G
UseLentiviral and Synthetic BiologyExpressionMammalianAvailable SinceJuly 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS DRR mCherry Donor EF1a BFP
Plasmid#98896PurposeHomologous recombination donor for pDRR. Expresses mCherry after repait by HR.DepositorInsertNeomycin-T2A-mCherry-GFP
UseSynthetic BiologyExpressionMammalianAvailable SinceNov. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-puro-TagBFP-β-ACTIN-3′UTR-24xMS2
Plasmid#205161Purposeexpress β-ACTIN-3′UTR-24xMS2DepositorInsertβ-ACTIN-3′UTR-24xMS2
TagstagBFPExpressionBacterial and MammalianPromoterCMVAvailable SinceOct. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
VEE-mScarlet3-IRES-EGFP-IRES-moxBFP
Plasmid#242407PurposeNative (conventional) self-amplifying RNA (saRNA) control construct expresses moxBFP as visual marker of IRES functionalityDepositorInsertsmScarlet3
IRES-EGFP
IRES-moxBFP
UseSelf-amplifying rnaExpressionMammalianAvailable SinceSept. 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
Str-2-TagBFP-SL2-Pre-SPHERE-SF-iGluSnFR
Plasmid#187099PurposeGlutamate sensor for localization to membrane contatctDepositorArticleInsertPre-SPHERE-SF-iGluSnFR
TagsTagBFP-SL2ExpressionWormPromoterStr-2Available SinceSept. 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
CROPseq-multi-v2-palmitoyl-mTagBFP2-BsmBI_entry (pRW1509)
Plasmid#225755PurposeEntry vector to clone sgRNA(s) into the CROPseq-multi-v2 construct with membrane-localized palmitoyl-mTagBFP2; v2 is compatible with T7 in vitro transcription detectionDepositorTypeEmpty backboneUseCRISPR and LentiviralExpressionMammalianAvailable SinceSept. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
LV anti-FITC-LaG16-Y186A-T2A-BFP
Plasmid#194932PurposeLV construct encoding Y186A mutant sNRR-based SynNotch receptorDepositorInsertantiFITC-LaG16-sNRR(Y186A)-SynNotch-Gal4-VP64-T2A-BFP
UseLentiviral and Synthetic BiologyAvailable SinceMarch 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
LV anti-FITC-LaG16-Y192A-T2A-BFP
Plasmid#194933PurposeLV construct encoding Y192A mutant sNRR-based SynNotch receptorDepositorInsertantiFITC-LaG16-sNRR(Y192A)-SynNotch-Gal4-VP64-T2A-BFP
UseLentiviral and Synthetic BiologyAvailable SinceMarch 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLV-UBC mTagBFP2-P2A-3xFLAG-hEEA1
Plasmid#214995PurposeExpress the human EndoTag (3xFLAG-EEA1) for EndoIPs. mTagBFP2 to sort for even expression levels.DepositorAvailable SinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKLV2.2-h7SKgRNA5(SapI)-hU6gRNA5(BbsI)-PGKpuroBFP-W
Plasmid#72666PurposeLentiviral dual CRISPR gRNA expression vector (human 7SK and human U6 promoters)DepositorInserth7SKgRNA cassette, hU6gRNA cassette, PGKpuroBFP cassette, WPRE
UseCRISPR and LentiviralExpressionMammalianAvailable SinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
TLCV2-ApaLI-FLAG-T2A-mTagBFP2-Puro
Plasmid#211462PurposeLentivirus backbone expressing a doxycycline-inducible mitochondrial localized ApaLIDepositorInsertApaLI
UseLentiviralTagsFLAGExpressionMammalianPromoterTREAvailable SinceDec. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCW-Tet-Cas9-BFP-PGK-Blast
Plasmid#196720PurposeTet-inducible expression of Cas9-T2A-TagBFPDepositorInsertCas9-T2A-TagBFP
UseCRISPR and LentiviralTags3xFLAG-SV40NLS and Nucleoplasmin NLSPromoterTRE, PGKAvailable SinceApril 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
Fuw-dCas9-dead Tet1CD-P2A-BFP
Plasmid#108246PurposeLentiviral vector to express dCas9-dead Tet1CD-P2A-tagBFPDepositorInsertdCas9-dead Tet1CD-HA-P2A-BFP
UseLentiviralTagsHAExpressionMammalianMutationdCas9-D10A, dCas9-H840A, Tet1CD-H1672Y, Tet1CD-D1…PromoterUbcAvailable SinceJune 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHBS1503 [IBB-GFP-mCherry3E]-[BFP-TDP43 WT]
Plasmid#133327PurposeTo test the effect of sequence on TDP43 splicing activityDepositorAvailable SinceNov. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
TLCV2-ApaLI*-FLAG-T2A-mTagBFP2-Puro
Plasmid#211463PurposeLentivirus backbone expressing a doxycycline-inducible mitochondrial localized ApaLI mutant (D211A, E225A and K227A)DepositorInsertApaLI-dead
UseLentiviralTagsFLAGExpressionMammalianMutationD211A, E225A, K227APromoterTREAvailable SinceDec. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
EK0441 CMV-TO-BFPmut-stop-EGFP.t2 (FLP-IN)
Plasmid#191161PurposeExpression of a trigger sequence from EGFP CDS in 3' UTR context in mammalian cellsDepositorInsertEGFP.t2
ExpressionMammalianMutationWTPromoterCMV-TOAvailable SinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6(gCD44v2)-EF1a-BFP-Puro-Cas9(FZ)
Plasmid#117134PurposeAll-in-one expression vector for Cas9 and gRNA against CD44DepositorInsertgCD44 v2 (Cd44 Synthetic)
UseCRISPR and LentiviralMutationDeleted BbsI site within WPRE, modified BFPPromoterhuman U6 promoter, human EF1a promoterAvailable SinceApril 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLenti-hU6-enhanced_gRNA-BFP-P2A-PuroR
Plasmid#230935PurposegRNA cloning vector encoding BFP and puromycin resistanceDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsTagBFPPromoterhU6Available SinceFeb. 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pKLV2.2-mU6gRNA5(SapI)-hU6gRNA5(BbsI)-PGKpuroBFP-W
Plasmid#72667PurposeLentiviral dual CRISPR gRNA expression vector (mouse U6 and human U6 promoters)DepositorInsertmU6gRNA cassette, hU6gRNA cassette, PGKpuroBFP cassette, WPRE
UseCRISPR and LentiviralExpressionMammalianAvailable SinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHR-UCOE-EF1a-Zim3-dCas9-BFP
Plasmid#188775PurposedCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLSDepositorInsertZim3-dCas9-mTagBFP2
UseLentiviralTagsHA-2xNLS-mTagBFP2 and Zim3 KRAB-NLS fusionPromoterEF1aAvailable SinceOct. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pU6-(BbsI)_CBh-Cas9-T2A-BFP
Plasmid#64323PurposeExpression vector for sgRNAs cloned into the BbsI sites and for expression of Cas9 linked to BFP via a T2A peptideDepositorInsertsCas9
sgRNA cassette
UseCRISPRTags3xFLAG, NLS, and T2A-EBFP2ExpressionMammalianPromoterCBh and U6Available SinceMay 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV Jph3gRNA mEmerald hSyn mTagBFP2-CAAX2
Plasmid#236246PurposeEndogenous tagging of Junctophilin 3 with mEmerald with a fluorescent marker for the plasma membraneDepositorInsertgRNA for rat Jph3, mEmerald donor and mTagBFP2-CAAX2
UseAAVTagsmTagBFP2PromoterU6 and human Synapsin1Available SinceMay 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHR hEF1A-BFP-P2A-rtTA-WPRE
Plasmid#223288PurposeA lentiviral vector expressing BFP and the TetON transcription factor rtTA.DepositorInsertBFP-P2A-rtTA
UseLentiviralExpressionMammalianAvailable SinceAug. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSLPB2B-FKBP12_F36V-CasRx-tagBFP-PGK-Blasticidin
Plasmid#187952PurposeFKBP12 (F36V mutant) degron-tagged CasRx fused with tagBFP, expressed under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance under PGK promoter.DepositorInsertFKBP12_F36V-CasRx-tagBFP
UseCRISPR and Synthetic BiologyTagsGGS linker, HA-tag, and NLSExpressionMammalianAvailable SinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHR-UCOE-SFFV-Zim3-dCas9-BFP
Plasmid#188776PurposedCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLSDepositorInsertZim3-dCas9-mTagBFP2
UseLentiviralTagsHA-2xNLS-mTagBFP2 and Zim3 KRAB-NLS fusionPromoterSFFVAvailable SinceOct. 18, 2022AvailabilityAcademic Institutions and Nonprofits only