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Plasmid#51283Purposeexpresses Alu, a human SINE, using the pol III enhancer sequence of the 7SL gene and tagged with the self splicing intron neo cassetteDepositorInsertAluYa5
UseTagsneoTET cassette with a tetrahymena self-splicing …ExpressionMammalianMutationPromoterupstream pol III enhancer sequence of the 7SL gen…Available sinceApril 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
GOMO-GFP
Plasmid#60975PurposeRapid selection of recombinant marker-free fluorescent rodent malaria parasitesDepositorInsertsGFP
mCherry
hDHFR-yFCU
UsePlasmodium gene targetingTagsExpressionMutationPromoterAvailable sinceApril 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFosCreER
Plasmid#46388DepositorInsertsUseMouse TargetingTagsExpressionMutationPromoterAvailable sinceJuly 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
pMK294 (mAID-mCherry2-Bsr)
Plasmid#72832PurposeC-terminal tagging with mAID-mCherry2 using CRISPR/Cas9DepositorInsertmAID-mCherry2-Bsr
UseCRISPRTagsmAID-mCherry2ExpressionMammalianMutationPromoterAvailable sinceFeb. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMK291 (mAID-mClover-Bsr)
Plasmid#72829PurposeC-terminal tagging with mAID-mClover using CRISPR/Cas9DepositorInsertmAID-mClover-Bsr
UseCRISPRTagsmAID-mCloverExpressionMammalianMutationPromoterAvailable sinceFeb. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBS-ACTB-C200-GFPfr1
Plasmid#169798PurposeTHe donor vector for the ACTB gene locus.DepositorInsertDonor sequence to ACTB gene for HR
UseHr donor vector for human actb gene.TagsExpressionMutationPartial sequence of GFP is inserted before the st…PromoterAvailable sinceNov. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
RIP Cre-ER (DM#265)
Plasmid#15029DepositorInsertRat insulin promoter (RIP) (Ins2 Rat)
UseCre/Lox; For making transgenic constructsTagsCre-ER (TM)ExpressionMammalianMutationPromoterAvailable sinceOct. 18, 2007AvailabilityAcademic Institutions and Nonprofits only -
pBS human c-Myc
Plasmid#14971DepositorInsertc-Myc (MYC Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 17, 2007AvailabilityAcademic Institutions and Nonprofits only -
pT-GEM(2)
Plasmid#62894PurposeTrojan-Gal4 Expression Module in Phase 2. Insert T2A-Gal4 to genomic loci using the Crispr/Cas technology. Can be converted by cassette exchange into other Trojan exonsDepositorTypeEmpty backboneUseTagsExpressionMutationPromoterAvailable sinceMay 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBSSK WT p85 alpha flag
Plasmid#13429DepositorInsertp85 alpha (PIK3R1 Human)
UseTagsflagExpressionBacterialMutationPromoterAvailable sinceNov. 1, 2006AvailabilityAcademic Institutions and Nonprofits only -
pT-GEM(1)
Plasmid#62893PurposeTrojan-Gal4 Expression Module in Phase 1. Insert T2A-Gal4 to genomic loci using the Crispr/Cas technology. Can be converted by cassette exchange into other Trojan exonsDepositorTypeEmpty backboneUseTagsExpressionMutationPromoterAvailable sinceMay 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTUB1:YFP-AID-3HA, DHFR-TS:HXGPRT
Plasmid#87260PurposeYFP-AID-3HA fusion driven by a minimal TUB1 promoter with an HXGPRT drug selectable marker. The AID CDS is Arabidopsis thaliana auxin-responsive protein IAA17.DepositorInsertsYFP-AID-3HA
HXGPRT
UseToxoplasma expressionTags3HA and AIDExpressionMutationPromoterTgDHFR-TS 463 bp and TgTUB1 378 bpAvailable sinceMay 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBS-KLF7-200-GFPfr1
Plasmid#169799PurposeTHe donor vector for the KLF7 gene locus.DepositorInsertDonor sequence to KLF7 neighbouring region for HR
UseHr donor vector for human actb gene.TagsExpressionMutationPartial sequence of GFP is inserted the sequence …PromoterAvailable sinceAug. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMK284 (Stag-3FLAG-Hygro)
Plasmid#72800PurposeC-terminal tagging with Stag-3FLAG using CRIPSR/Cas9DepositorInsertStag-3FLAG-Hygro
UseCRISPRTagsStag-3FLAGExpressionMammalianMutationPromoterAvailable sinceMarch 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
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pMK283 (Stag-3FLAG-Neo)
Plasmid#72799PurposeC-terminal tagging with Stag-3FLAG using CRIPSR/Cas9DepositorInsertStag-3FLAG-Neo
UseCRISPRTagsStag-3FLAGExpressionMammalianMutationPromoterAvailable sinceMarch 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
Spy CCR5-pegRNA
Plasmid#169856PurposeSpyCas9-pegRNA for CCR5DepositorInsertSpy CCR5-pegRNA
UseTagsExpressionMammalianMutationPromoterhuman U6 promoterAvailable sinceJune 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMK280 (mCherry2-NeoR)
Plasmid#72796PurposeC-terminal tagging with mCherry2 using CRIPSR/Cas9DepositorInsertmCherry2-Bsr
UseCRISPRTagsmCherry2ExpressionMammalianMutationPromoterAvailable sinceMarch 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBS ColI alpha2
Plasmid#19207DepositorInsertAlpha 2 type I collagen (COL1A2 Chicken)
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 19, 2008AvailabilityAcademic Institutions and Nonprofits only -
pHB9-MCS-IRES-EGFP (mHb9 promoter) [TJ#103]
Plasmid#16283DepositorInsertHB9 promoter (Mnx1 Mouse)
UseMouse TargetingTagsIRES-EGFPExpressionMammalianMutationPromoterAvailable sinceFeb. 4, 2009AvailabilityAcademic Institutions and Nonprofits only -
pBS NL4-3 envFS eGFP Δrev
Plasmid#101348PurposeAll mutations in rev are silent with respect to the tat reading frame. Start ATG->ACG and nts 68-71 were mutated AGC->TCA to change tyrosine to a stop.DepositorInsertNL4-3
UseLentiviralTagsExpressionMutationdelta revPromoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBS-IT6
Plasmid#66870PurposeInntag 6: a small self-structured epitope aimed at innocuous protein taggingDepositorInsertInntag 6
UseTagsExpressionMutationPromoterAvailable sinceAug. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
Axin2 probe
Plasmid#21277DepositorInsertAxin2 (Axin2 Mouse)
UseFor riboprobe synthesisTagsExpressionMutationcDNA bp 1774 to 2787PromoterAvailable sinceJune 9, 2009AvailabilityAcademic Institutions and Nonprofits only -
pBS hBAF53a
Plasmid#17879DepositorInsertBAF53a (ACTL6A Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 29, 2008AvailabilityAcademic Institutions and Nonprofits only -
pMK277 (mClover-NeoR)
Plasmid#72793PurposeC-terminal tagging with mClover using CRIPSR/Cas9DepositorInsertmClover-Neo
UseCRISPRTagsmCloverExpressionMammalianMutationPromoterAvailable sinceMarch 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBS mouse c-Myc
Plasmid#14972DepositorInsertc-Myc (Myc Mouse)
UseTagsExpressionBacterialMutationNucleotides 2578-3294PromoterAvailable sinceMay 17, 2007AvailabilityAcademic Institutions and Nonprofits only -
pMK285 (Stag-3FLAG-Bsr)
Plasmid#72801PurposeC-terminal tagging with Stag-3FLAG using CRIPSR/Cas9DepositorInsertStag-3FLAG-Bsr
UseCRISPRTagsStag-3FLAGExpressionMammalianMutationPromoterAvailable sinceMarch 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
SpyCas9 EMX1-nicking sgRNA
Plasmid#169859PurposeSpyCas9 nicking sgRNA for EMX1DepositorInsertSpyCas9 EMX1-nicking sgRNA
UseTagsExpressionMammalianMutationPromoterhuman U6 promoterAvailable sinceJune 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPEE47
Plasmid#139426PurposeRecyclable marker for use in Cryptococcus neoformans. Contains the amdS2 counterselectable marker flanked by the mNeonGreen ORF and a flexible linker at the 5' end.DepositorInsertmNeonGreen
UseTagsExpressionYeastMutationPromoterTEF1Available sinceMay 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPEE46
Plasmid#139425PurposeRecyclable marker for use in Cryptococcus neoformans. Contains the amdS2 counterselectable marker flanked by the mRuby3 ORF and a flexible linker at the 5' end.DepositorInsertmRuby3
UseTagsExpressionYeastMutationPromoterTEF1Available sinceMay 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
Spy EMX1-pegRNA
Plasmid#169855PurposeSpyCas9-pegRNA for EMX1DepositorInsertSpy EMX1-pegRNA
UseTagsExpressionMammalianMutationPromoterhuman U6 promoterAvailable sinceJune 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBS-IT5
Plasmid#66869PurposeInntag 5: a small self-structured epitope aimed at innocuous protein taggingDepositorInsertInntag 5
UseTagsExpressionMutationPromoterAvailable sinceAug. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMCS.71/2272.Hygro
Plasmid#22731DepositorInsertLox71-FRT-PGK-hygro-FRT-Lox2272
UseCre/LoxTagsExpressionMutationThis is a basal exchange vector for RMCE. The vec…PromoterAvailable sinceDec. 14, 2009AvailabilityAcademic Institutions and Nonprofits only -
SauCas9 EMX1-pegRNA
Plasmid#169857PurposeSauCas9 pegRNA for EMX1DepositorInsertSauCas9 EMX1-pegRNA
UseTagsExpressionMammalianMutationPromoterhuman U6 promoterAvailable sinceJune 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
Ctcf-Halo-mAID donor
Plasmid#113103PurposeDonor plasmid for knock-in mAID-Halo fusion to the c-terminal of mouse Ctcf coding sequenceDepositorInsertc terminal Ctcf donor region including homology arms and mAID-HaloTag (Ctcf Mouse, Synthetic)
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJC30X-BS(X)
Plasmid#58958PurposeEncodes synthetic mitochondrial gene GFPm-3 in place of COX3 coding sequence. Flanked by mtDNA sequences allowing integration into rho+ mtDNA after transformation.DepositorInsertEnhanced fluorescence variant of GFP, in S. cerevisiae mitochondrial genetic code
UseTagsFirst 8 codons of COX3ExpressionMutationGFP modified as described: CORMACK, B. P., R. H. …Promotern/aAvailable sinceSept. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
SauCas9 EMX1-nicking sgRNA
Plasmid#169860PurposeSauCas9 nicking sgRNA for EMX1DepositorInsertSauCas9 EMX1-nicking sgRNA
UseTagsExpressionMammalianMutationPromoterhuman U6 promoterAvailable sinceJune 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBT305_pBS-U-attPx3-FRT5-pSV40-Neo-pA-PL-FRT5
Plasmid#52539PurposeExpression of FRT5-flanked Neo; homologous recombination targeting vector cloningDepositorInsertsPhiC31o attPx3 (70 bp each)
Neo
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCA-G-intron-G
Plasmid#40027DepositorInsertsGFP exon 1
GFP exon 2
beta-globin intron
UseTagsExpressionMammalianMutationinsertion of a loxP site, nucleotides 1-274, and …PromoterCAG (chicken beta globin promoter and CMV enhance…Available sinceOct. 12, 2012AvailabilityAcademic Institutions and Nonprofits only -