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Showing: 101 - 150 of 426 results

  19. pLenti-NIrD

    Plasmid
    #65010
    Purpose
    NS3/4A Inducible rtTA-mediated Dual-reporter system for Hepatitis C Virus (HCV) infection
    Depositor
    Wensheng Wei
    Article
    Ren et al Sci Rep. 2015 Mar 9;5:8865. doi: 10.1038
    Inserts
    rtTA-MAVS(C)
    2A-linked delta-TK
    Use
    Lentiviral
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    Tight-TRE
    Available since
    Dec. 15, 2015
    Availability
    Academic Institutions and Nonprofits only

  23. pCAS

    Plasmid
    #60847
    Purpose
    Expresses S. pyogenes Cas9 plus an HDV ribozyme-sgRNA for genome editing in yeast
    Depositor
    Jamie Cate
    Article
    Ryan et al Elife. 2014 Aug 19;3. doi: 10.7554/eLife
    Inserts
    S. pyogenese Cas9
    RNA pol III promoter (tRNA-Tyr)
    hepatitis delta virus ribozyme, genomic
    sgRNA
    Use
    CRISPR and Synthetic Biology
    Tags
    NLS/His8 and TTT 3' extension prior to sgRNA
    Expression
    Bacterial and Yeast
    Mutation
    L4 is UUCG tetraloop and guide targets LYP1 (CATA…
    Promoter
    Available since
    Jan. 13, 2015
    Availability
    Academic Institutions and Nonprofits only
    60847-plasmid-map-sequence-id-197511

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  29. 1.3xWT HBV-Luciferase

    Plasmid
    #61549
    Purpose
    The 1.3xHBV-Luciferase plasmid to monitor HBV transcription. 1. Luciferase inserted within Core ORF. 2. Luciferase is regulated by EnhII - Core promoter. 3. The functional luciferase ATG is of Core
    Depositor
    Yosef Shaul
    Article
    Doitsh et al Mol Cell Biol. 2004 Feb;24(4):1799-808.
    Insert
    1.3 times of the HBV genome with luciferase within the Core ORF
    Use
    Luciferase
    Tags
    Expression
    Mammalian
    Mutation
    Substitution of HBV sequences with luciferase ORF…
    Promoter
    Available since
    June 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    61549-plasmid-map-sequence-id-212455

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  38. pEF NS34a-msGFP-'S-mito

    Plasmid
    #158765
    Purpose
    Expression of self-cleaving membrane targeting peptide containing the OMP25 C-terminal targeting sequence, the P6P4 NS5A/5B cleavage site, msGFP and the NS3/4A protease.
    Depositor
    Edwin Chapman
    Article
    Vevea et al eLife. 2020 Jun 9;9. pii: 56469. doi: 10
    Insert
    NS3/4a protease and msGFP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Flexible linkers between the protease and msGFP a…
    Promoter
    Available since
    Feb. 12, 2021
    Availability
    Academic Institutions and Nonprofits only
    158765-plasmid-map-sequence-id-313128

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  40. pEF NS34a-msGFP-A'Q-mito

    Plasmid
    #158763
    Purpose
    Expression of self-cleaving membrane targeting peptide containing the OMP25 C-terminal targeting sequence, a modified P6P4 NS5A/5B cleavage site, msGFP and the NS3/4A protease.
    Depositor
    Edwin Chapman
    Article
    Vevea et al eLife. 2020 Jun 9;9. pii: 56469. doi: 10
    Insert
    NS3/4a protease and msGFP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Flexible linkers between the protease and msGFP a…
    Promoter
    Available since
    Feb. 12, 2021
    Availability
    Academic Institutions and Nonprofits only
    158763-plasmid-map-sequence-id-312944

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  41. pEF NS34a-msGFP-'Q-mito

    Plasmid
    #158764
    Purpose
    Expression of self-cleaving membrane targeting peptide containing the OMP25 C-terminal targeting sequence, a modified P6P4 NS5A/5B cleavage site, msGFP and the NS3/4A protease.
    Depositor
    Edwin Chapman
    Article
    Vevea et al eLife. 2020 Jun 9;9. pii: 56469. doi: 10
    Insert
    NS3/4a protease and msGFP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Flexible linkers between the protease and msGFP a…
    Promoter
    Available since
    Feb. 12, 2021
    Availability
    Academic Institutions and Nonprofits only
    158764-plasmid-map-sequence-id-312950

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  44. pF(UG) hSyn NS34a-msGFP-A'Q-mito

    Plasmid
    #158766
    Purpose
    Lentiviral expression of self-cleaving membrane targeting peptide containing the OMP25 C-terminal targeting sequence, a modified P6P4 NS5A/5B cleavage site, msGFP and the NS3/4A protease.
    Depositor
    Edwin Chapman
    Article
    Vevea et al eLife. 2020 Jun 9;9. pii: 56469. doi: 10
    Insert
    NS3/4a protease and msGFP
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Flexible linkers between the protease and msGFP a…
    Promoter
    Available since
    Availability
    Academic Institutions and Nonprofits only
    158766-plasmid-map-sequence-id-313130

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  45. pF(UG) hSyn NS34a-msGFP-'Q-mito

    Plasmid
    #158767
    Purpose
    Lentiviral expression of self-cleaving membrane targeting peptide containing the OMP25 C-terminal targeting sequence, a modified P6P4 NS5A/5B cleavage site, msGFP and the NS3/4A protease.
    Depositor
    Edwin Chapman
    Article
    Vevea et al eLife. 2020 Jun 9;9. pii: 56469. doi: 10
    Insert
    NS3/4a protease and msGFP
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Flexible linkers between the protease and msGFP a…
    Promoter
    Available since
    Availability
    Academic Institutions and Nonprofits only
    158767-plasmid-map-sequence-id-313145

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  46. pF(UG) hSyn NS34a-msGFP-'S-mito

    Plasmid
    #158768
    Purpose
    Lentiviral expression of self-cleaving membrane targeting peptide containing the OMP25 C-terminal targeting sequence, the P6P4 NS5A/5B cleavage site, msGFP and the NS3/4A protease.
    Depositor
    Edwin Chapman
    Article
    Vevea et al eLife. 2020 Jun 9;9. pii: 56469. doi: 10
    Insert
    NS3/4a protease and msGFP
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Flexible linkers between the protease and msGFP a…
    Promoter
    Available since
    Availability
    Academic Institutions and Nonprofits only
    158768-plasmid-map-sequence-id-313146

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  48. pCS6-YFP-SMASh(AI)

    Plasmid
    #111500
    Purpose
    Expresses Venus YFP with a C-terminal SMASh(AI) tag fusion in mammalian cells. [AI = asunaprevir inhibited].
    Depositor
    Michael Lin
    Article
    Jacobs et al Nat Methods. 2018 Jul;15(7):523-526. doi
    Insert
    YFP-SMASh(AI)
    Use
    Tags
    HA epitope tag and SMASh tag
    Expression
    Mammalian
    Mutation
    The HCV NS3 protease carries V36M, T54A, and S122…
    Promoter
    CMV
    Available since
    July 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    111500-plasmid-map-sequence-id-214262

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  49. pCS6-YFP-SMASh(TI)

    Plasmid
    #111501
    Purpose
    Expresses Venus YFP with a C-terminal SMASh(TI) tag fusion in mammalian cells. [TI = telaprevir inhibited].
    Depositor
    Michael Lin
    Article
    Jacobs et al Nat Methods. 2018 Jul;15(7):523-526. doi
    Insert
    YFP-SMASh(TI)
    Use
    Tags
    HA epitope tag and SMASh tag
    Expression
    Mammalian
    Mutation
    The HCV NS3 protease carries F43L, Q80K, S122R, a…
    Promoter
    CMV
    Available since
    July 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    111501-plasmid-map-sequence-id-214268

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Showing: 101 - 150 of 426 results