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Showing: 101 - 150 of 17125 results

  1. B52_puro_empty_gRNA

    Plasmid
    #197557
    Purpose
    sites for cloning two gRNA with puromycin cassette
    Depositor
    Darius Bägli
    Type
    Empty backbone
    Use
    Empty grna backbone with puromycin resistance gene
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6 for gRNA, CMV for puromycin resistance.
    Available since
    March 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    197557-plasmid-map-sequence-id-388707

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  11. pAK_Tol2_TRE_Puro

    Plasmid
    #130259
    Purpose
    Tol2 doxycycline inducible expression vector
    Depositor
    Michael Yaffe
    Type
    Empty backbone
    Use
    Tol2 transposon, doxycycline inducible
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    TRE Promoter
    Available since
    Aug. 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    130259-plasmid-map-sequence-id-255016

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  13. AAVS1_puro_CAG_HexaPro

    Plasmid
    #164077
    Purpose
    Targeted integration of SARS-CoV-2 spike HexaPro variant into the AAVS1 genomic safe harbor locus
    Depositor
    Yannick Doyon
    Article
    Rivest et al protocols.io 2021
    Insert
    SARS-CoV-2 S HexaPro (S Severe acute respiratory syndrome coronavirus 2)
    Use
    CRISPR
    Tags
    2X Strep-Tag II, 8X His tag, and HRV 3C cleavage …
    Expression
    Mammalian
    Mutation
    Ectodomain only (AAs 1-1208); 682-685 (furin site…
    Promoter
    CAG
    Available since
    Jan. 4, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    164077-plasmid-map-sequence-id-320680

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  16. pucKan_ARS_pgkZeo_ncx1_mCherry_2TA_Puro_ARS

    Plasmid
    #163755
    Purpose
    piggybac transposon encoding human Ncx1 gene promoter-driven mCherry and puromycin-acetyltransferase for labelling and selection of pluripotent stem cell-derived cardiomyocytes
    Depositor
    Kurt Pfannkuche
    Article
    Hamad et al Methods Mol Biol. 2021 Oct 19. doi: 10.1
    Insert
    mCherry
    Use
    Piccybac transposon
    Tags
    puromycin-N-acetyltransferase
    Expression
    Mammalian
    Mutation
    Promoter
    human NCX1
    Available since
    March 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    163755-plasmid-map-sequence-id-326668

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  18. Flag_HsIRE1a_deltaP29_D408_pBabePuro

    Plasmid
    #58422
    Purpose
    retroviral pBabe puro vector encoding N-terminally FLAG tagged mutant human IRE1a deleted from amino acids P28 to D408
    Depositor
    David Ron
    Article
    Volmer et al Proc Natl Acad Sci U S A. 2013 Mar 19;11
    Insert
    IRE1a (ERN1 Human)
    Use
    Retroviral
    Tags
    FLAG and preprotrypsin signal sequence
    Expression
    Mammalian
    Mutation
    Deleted amino acids P29 to D408
    Promoter
    Available since
    Sept. 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    58422-plasmid-map-sequence-id-90253

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  21. Flag_HsIRE1a_deltaP29_D408_K599A_pBabePuro

    Plasmid
    #58492
    Purpose
    retroviral pBabe puro vector encoding N-terminally FLAG tagged mutant human IRE1a deleted from amino acids P28 to D408 and bearing mutation K599A (kinase domain mutant)
    Depositor
    David Ron
    Article
    Volmer et al Proc Natl Acad Sci U S A. 2013 Mar 19;11
    Insert
    IRE1a (ERN1 Human)
    Use
    Retroviral
    Tags
    FLAG and preprotrypsin signal sequence
    Expression
    Mammalian
    Mutation
    deleted amino acids P29 to D408 and bearing mutat…
    Promoter
    Available since
    Sept. 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    58492-plasmid-map-sequence-id-90361

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  28. OmEF1aCas9P2ApuroSB

    Plasmid
    #165484
    Purpose
    Stable expression of a zebrafish optimized Cas9 driven by a tilapia promoter in fish cells
    Depositor
    Dietmar Kueltz
    Article
    Hamar et al Sci Rep. 2021 Apr 12;11(1):7854. doi: 10
    Insert
    Zebrafish optimized Cas9 (nls-zcas9-nls from Wenbiao Chen Lab plasmid pCS2-nCas9n Addgene #47929)
    Use
    CRISPR; Transposon
    Tags
    Expression
    Mutation
    Promoter
    Oreochromis mossambicus EF1 alpha promoter (OmEF1…
    Available since
    May 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    165484-plasmid-map-sequence-id-330622

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  32. pAK_lentiguide_DR30_Puro

    Plasmid
    #134839
    Purpose
    Lentiviral expression CasRx guideRNA
    Depositor
    Michael Yaffe
    Insert
    CasRx guideRNA backbone
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    hU6
    Available since
    Nov. 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    134839-plasmid-map-sequence-id-265965

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  39. pMSCVpuroATT

    Plasmid
    #47539
    Purpose
    Retroviral Gateway destination vector constructed by inserting Reading Frame Cassette A into pMSCVpuro (Clontech) using the Gateway Vector Conversion System (Invitrogen)
    Depositor
    David Weinstock
    Article
    Shindoh et al PLoS One. 2012;7(11):e49201. doi: 10.137
    Type
    Empty backbone
    Use
    Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Sept. 25, 2013
    Availability
    Academic Institutions and Nonprofits only

  42. pLenti.puro.MCV.ER.RAZ.2

    Plasmid
    #114382
    Purpose
    This lentiviral plasmid expresses MCV tumor T antigens (truncated LT and small T) using the viral NCCR (non-coding control region). The sequence was amplifeied from MCV.R17a and truncated LT=428 aa.
    Depositor
    Reety Arora, Sudhir Krishna
    Article
    Arora et al Microb Pathog. 2019 Dec;137:103778. doi:
    Insert
    MCV NCCR and tumor T antigen regions
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    MCV NCCR (viral own promoter)
    Available since
    Sept. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    114382-plasmid-map-sequence-id-221614

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Showing: 101 - 150 of 17125 results