We narrowed to 2,905 results for: BFP

1. LV anti-FITC-LaG16-NRR-T2A-BFP

   Plasmid

   #194929

   Purpose

   LV construct encoding NRR-based SynNotch receptor

   Depositor

   John Ngo

   Article

   Sloas et al Nat Biotechnol. 2023 Jan 16. doi: 10.103

   Insert

   antiFITC-LaG16-NRR-SynNotch-Gal4-VP64-T2A-BFP
   

   Use

   Lentiviral and Synthetic Biology

   Available Since

   March 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLVX-TRE3G-BCCP-PupE-IRES-BFP

   Plasmid

   #113405

   Purpose

   Express inducible BCCP-PupE in mammalian cell

   Depositor

   Min Zhuang

   Article

   Liu et al Nat Methods. 2018 Aug 13. pii: 10.1038/s

   Insert

   BCCP-PupE-IRES-BFP
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Aug. 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJM456 EF1alpha EBFP2-P2A-BlastR (TUPV7)

   Plasmid

   #139236

   Purpose

   mMoClo TUPV7, with EF1alpha promoter and EBFP2-P2A-BlastR in the MCS

   Depositor

   Joshua Leonard

   Article

   Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

   Insert

   EBFP2-P2A-Blasticidin S Deaminase
   

   Use

   Synthetic Biology

   Expression

   Mammalian

   Promoter

   EF1alpha

   Available Since

   Feb. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pKE12-ISceI-fabp10a:loxP-BFP-loxP-Xla.Ctnnb1

   Plasmid

   #198240

   Purpose

   For I-SceI-mediated transgenesis in zebrafish; fabp10a promoter drives expression of activated β-catenin preceded by a blue fluorescent protein (BFP)-STOP cassette flanked by loxP sites

   Depositor

   Didier Stainier

   Article

   Kalasekar et al Biol Open. 2019 Oct 17;8(10):bio047829.

   Inserts

   Zebrafish fabp10a promoter:floxedBFP, Xenopus pt-beta-catenin coding sequence, polyA (fabp10a Frog)
   Zebrafish cryaa promoter:mcherry fluorescent protein coding sequence (cryaa Zebrafish)
   

   Use

   Zebrafish transgenesis

   Available Since

   July 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. Crop-Seq-BFP-WPRE-TS-hU6-BsmbI

   Plasmid

   #137993

   Purpose

   Backbone for generation of ClonMapper gRNA barcode library for use in droplet-based sequencing

   Depositor

   Amy Brock

   Article

   Gardner et al Methods Mol Biol. 2022;2394:109-131. doi

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. loxP-myctagBFP-STOP-loxP-mCherry-NTR

   Plasmid

   #108456

   Purpose

   This plasmid can be used to recombine the loxP-myctagBFP-STOP-loxP-mCherry-NTR cassette (or only mCherry-NTR) into a BAC

   Depositor

   Nadia Mercader Huber

   Article

   Sanchez-Iranzo et al Nat Commun. 2018 Jan 30;9(1):428. doi: 1

   Insert

   loxP-myctagBFP-STOP-loxP-mCherry-NTR
   

   Use

   Cre/Lox

   Available Since

   June 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEJS1084_pTetR-P2A-BFP/BspMI flexible sgRNA

   Plasmid

   #117687

   Purpose

   U6 driven Spy sgRNA cloning vector where guide sequences are inserted between BspMI sites. This plasmid works with Addgene #108570 for subnuclear proteomic profiling via C-BERST method.

   Depositor

   Erik Sontheimer

   Article

   Gao et al Methods Enzymol. 2019;616:365-383. doi:

   Inserts

   Kanamycin cassette
   TetR-P2A-BFP
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6 and hPGK

   Available Since

   Dec. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHR-EF1a-dCas9-HA-BFP-KRAB-NLS

   Plasmid

   #102244

   Purpose

   Lentiviral expression of dCas9-HA-BFP-KRAB-NLS for CRISPRi driven by a hEF1alpha promoter

   Depositor

   Jacob Corn

   Insert

   dCas9-HA-BFP-KRAB-NLS
   

   Use

   CRISPR and Lentiviral

   Tags

   HA-TagBFP-KRAB-NLS

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Oct. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHR_5xUAS-ETV2-P2A-BFP-WPRE_PGK-HygR

   Plasmid

   #216664

   Purpose

   Expressed Gal4-inducible ETV2 and BFP, with hygromycin resistance

   Depositor

   Leonardo Morsut

   Inserts

   ETV2 (Etv2 Mouse)
   BFP
   HygromycinR
   

   Use

   Lentiviral

   Available Since

   March 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. LV anti-FITC-LaG16-sNRR-T2A-BFP

    Plasmid

    #194930

    Purpose

    LV construct encoding sNRR-based SynNotch receptor

    Depositor

    John Ngo

    Article

    Sloas et al Nat Biotechnol. 2023 Jan 16. doi: 10.103

    Insert

    antiFITC-LaG16-sNRR-SynNotch-Gal4-VP64-T2A-BFP
    

    Use

    Lentiviral and Synthetic Biology

    Available Since

    March 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAG-bpNLS-hPol eta-2A-tagBFP-bGHpA

    Plasmid

    #193968

    Purpose

    Encodes a wildtype human Pol? (eta) with P2A-tagBFP marker driven by CAG promoter

    Depositor

    Huawei Tong

    Article

    Tong et al Nat Biotechnol. 2023 Jan 9. doi: 10.1038

    Insert

    hPol eta (POLH Human)
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Jan. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLV-EF1a-mTagBFP2-2A-Puro_hU6-DJ-DR36-BsmBI

    Plasmid

    #226009

    Purpose

    DjCas13d guide RNA cloning backbone (lenti)

    Depositor

    Silvana Konermann

    Article

    Wei et al Cell Syst. 2023 Dec 20;14(12):1087-1102.

    Type

    Empty backbone

    Use

    Lentiviral

    Available Since

    Oct. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRhobin2-SEC61B-IRES-NLS-mTagBFP-2xNLS

    Plasmid

    #239024

    Purpose

    Co-overexpression of Rhobin2-SEC61B fusion protein and NLS-mTagBFP-2xNLS as a nuclear labeling marker

    Depositor

    Bo Huang, William DeGrado

    Article

    Chen et al bioRxiv 2025.06.24.661379

    Insert

    Rhobin2-SEC61B (SEC61B Human, Synthetic)
    

    Tags

    TagBFP

    Expression

    Mammalian

    Promoter

    CMV promoter

    Available Since

    June 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. lenti-UCOE-dCas9-BFP-Zim3-KRAB

    Plasmid

    #196716

    Purpose

    CRISPR-inhibition. dCas9-KRAB(ZIM3) with TagBFP for sorting. Improved KRAB domain from ZIM3

    Depositor

    Bernhard Schmierer

    Insert

    dCas9-TagBFP-KRAB(ZIM3)
    

    Use

    CRISPR and Lentiviral

    Tags

    Nucleoplasmin NLS and SV40 NLS

    Mutation

    D10A, N863A

    Promoter

    EF1a

    Available Since

    April 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pKLV2.2-h7SKgRNA5(SMARCA4(43))-hU6gRNA5(SMARCA2(46))-PGKpuroBFP-W

    Plasmid

    #200504

    Purpose

    Lentiviral vector expressing gRNA targeting human SMARCA4 and SMARCA2

    Depositor

    Kosuke Yusa

    Article

    Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

    Insert

    SMARCA4(43), SMARCA2(46) (SMARCA2, SMARCA4 Human)
    

    Use

    Lentiviral

    Available Since

    Jan. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. EK0208 CMV-TO-BFPmut-stop-t70587 (FLP-IN)

    Plasmid

    #191138

    Purpose

    Expression of Trigger 1 in 3' UTR of an inactive BFP in mammalian cells

    Depositor

    Xiaojing Gao

    Article

    Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

    Insert

    t70587
    

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    CMV-TO

    Available Since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV hSyn INTRON mTagBFP2-CAAX2 WPRE

    Plasmid

    #236231

    Purpose

    AAV expression of a fluorescent marker, mTagBFP2, fused to the plasma membrane targeting sequence CAAX2

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Benedetti et al Cell. 2025 Jan 23;188(2):484-500.e22. do

    Insert

    mTagBFP2 fused to the plasma membrane targeting sequence CAAX2
    

    Use

    AAV

    Tags

    mTagBFP2

    Promoter

    human Synapsin 1

    Available Since

    May 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. EK0304 CMV-TO-BFPmut-stop-Bdnf.t1 (FLP-IN)

    Plasmid

    #191146

    Purpose

    Expression of only the trigger sequence for Bdnf.s1 (EK0301) in mammalian cells

    Depositor

    Xiaojing Gao

    Article

    Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

    Insert

    Bdnf.t1
    

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    CMV-TO

    Available Since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA-puro-TagBFP-β-ACTIN-3′UTR-8xMS2

    Plasmid

    #205160

    Purpose

    express β-ACTIN-3′UTR-8xMS2

    Depositor

    Weirui Ma

    Article

    Hu et al Elife. 2023 Mar 3;12:e82178. doi: 10.755

    Insert

    β-ACTIN-3′UTR-24xMS2
    

    Tags

    tagBFP

    Expression

    Bacterial and Mammalian

    Promoter

    CMV

    Available Since

    Oct. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCF525-EF1a-Hygro-P2A-mTagBFP2-lenti

    Plasmid

    #115797

    Purpose

    Expresses mTagBFP in human cells, contained in a lentiviral vector.

    Depositor

    Jennifer Doudna

    Article

    Watters et al Science. 2018 Oct 12;362(6411):236-239.

    Insert

    mTagBFP
    

    Use

    Lentiviral

    Expression

    Bacterial and Mammalian

    Promoter

    EF-1a promoter

    Available Since

    Oct. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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21. pME-3xNLS-mTagBFP2-Cre (JDW 918)

    Plasmid

    #224517

    Purpose

    A Gateway-compatible middle entry clone containing a 3xNLS-mTagBFP2-Cre fusion

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    3xNLS-mTagBFP2-Cre
    

    Use

    Cre/Lox

    Available Since

    Sept. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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22. pLV-EF1a-mTagBFP2-2A-Puro_hU6-RfxDR36-BsmBI

    Plasmid

    #226011

    Purpose

    CasRx guide RNA cloning backbone (lenti)

    Depositor

    Silvana Konermann

    Article

    Wei et al Cell Syst. 2023 Dec 20;14(12):1087-1102.

    Type

    Empty backbone

    Use

    Lentiviral

    Available Since

    Oct. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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23. lentiCRISPRv2-sgRNAs-del-CDK5RAP2-SVA-BFP

    Plasmid

    #202824

    Purpose

    Lentiviral vector modified to express two sgRNAs that delete the intronic SVA within the CDK5RAP2 gene with EF-1apha for Cas9 and BFP expression

    Depositor

    Matthew Anderson

    Article

    Nadler et al Commun Biol. 2023 Mar 30;6(1):347. doi:

    Insert

    two sgRNAs (each with a U6 promoter) to delete SVA within CDK5RAP2 gene
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    U6 - two

    Available Since

    July 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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24. pU6-sgRNA EF1Alpha-puro-T2A-BFP.CYRANO

    Plasmid

    #128755

    Purpose

    Expresses CYRANO gRNA for CRISPRi

    Depositor

    David Bartel

    Article

    Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

    Insert

    CYRANO sgRNA (OIP5-AS1 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    mU6

    Available Since

    Aug. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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25. pcg 2.0-BFP-circ-arRNA backbone

    Plasmid

    #213965

    Purpose

    pcg 2.0-BFP-circ-arRNA backbone included a Twister P3 U2A, 5’ ligation sequence, a 3’ ligation sequence and Twister P1 based on pLenti-sgRNA-lib 2.0 backbone (Addgene no. 89638). Then, the sequences o

    Depositor

    Wensheng Wei

    Article

    Yi et al Nat Biotechnol. 2022 Jun;40(6):946-955.

    Type

    Empty backbone

    Expression

    Mammalian

    Available Since

    Feb. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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26. pCMV-AID-PcrA-UGI-T2A-mTagBFP2

    Plasmid

    #221224

    Purpose

    Express HACE Editor as a fusion of AID cytidine deaminase and PcrA helicase with mTagBFP2 reporter

    Depositor

    Fei Chen

    Article

    Chen et al Science. 2024 Oct 11;386(6718):eadn5876.

    Insert

    AID-PcrA-UGI-T2A-mTagBFP2
    

    Expression

    Mammalian

    Available Since

    Feb. 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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27. pAAV-anti-GFP/BFP(LaG2) PAGER(FL)

    Plasmid

    #230022

    Purpose

    Expresses anti-GFP/BFP PAGER(FL) in mammalian cells

    Depositor

    Alice Ting

    Article

    Kalogriopoulos et al Nature. 2024 Dec 4. doi: 10.1038/s41586-

    Insert

    pAAV-MT3-LaG2-TEVcs-ALFA-hM4DxcpGFP
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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28. L1T10 - T2PB/pUBQ10 (mTagBFP2, mCherry, NeonGreen)

    Plasmid

    #219687

    Purpose

    Level 1 History-dependent target, switch PhiC31 then Bxb1, pUBQ10 promoter, and output: mtagBFP2 then mCherry then NeonGreen (for transformation, Kan plant resistance, Kan bacteria resistance).

    Depositor

    Jennifer Nemhauser

    Insert

    T2PB/pUBQ10 (mTagBFP2, mCherry, NeonGreen)
    

    Expression

    Plant

    Available Since

    July 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. G417A_pLenti-Tet-BattP(GA)-BFP-2A-iCasp9-2A-Blast_rtTA3

    Plasmid

    #200631

    Purpose

    Negative selection blasticidin resistant Tet-inducible Matreyek Bxb1(GA) landing pad

    Depositor

    Kenneth Matreyek

    Article

    Roelle et al ACS Synth Biol. 2023 Nov 3. doi: 10.1021

    Insert

    iCasp9
    

    Use

    Lentiviral

    Promoter

    pTRE3G

    Available Since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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30. pTRIP-SFFV-mtagBFP-2A STING V155M 368STOP

    Plasmid

    #102596

    Purpose

    Lentivector to express STING V155M delta 368 and mTagBFP

    Depositor

    Nicolas Manel

    Article

    Cerboni et al J Exp Med. 2017 Jun 5;214(6):1769-1785.

    Insert

    STING (STING1 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    V155M delta 368

    Promoter

    SFFV

    Available Since

    Jan. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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31. pTUB1-Sth.dCas9-P2A-CAT-T2A-TagBFP

    Plasmid

    #171090

    Purpose

    CRISPR interference. Construct for expressing catalytically inactive Cas9 (dCas9) from Streptococcus thermophilus (CRISPR1 locus) in Toxoplasma gondii. Suitable for generating stable cell lines.

    Depositor

    Sebastian Lourido

    Article

    Markus et al mSphere. 2021 Oct 13:e0047421. doi: 10.1

    Inserts

    Sth1 dCas9
    Chloramphenicol acetyltransferase
    TagBFP
    

    Use

    CRISPR; Toxoplasma gondii expression

    Tags

    3xFLAG, P2A peptide, SV40 NLS, T2A peptide, and n…

    Mutation

    D9A, H599A

    Promoter

    TUB1 (Toxoplasma gondii), transcriptionally linke…

    Available Since

    Oct. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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32. pTRIP-SFFV-mtagBFP-2A STING-V155M S366A

    Plasmid

    #102593

    Purpose

    Lentivector to express STING V155M S366A and mTagBFP

    Depositor

    Nicolas Manel

    Article

    Cerboni et al J Exp Med. 2017 Jun 5;214(6):1769-1785.

    Insert

    STING (STING1 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    V155M S366A

    Promoter

    SFFV

    Available Since

    April 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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33. pU6-sgRNA EF1Alpha-puro-T2A-BFP.Control

    Plasmid

    #128749

    Purpose

    Expresses control gRNA

    Depositor

    David Bartel

    Article

    Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

    Insert

    Control sgRNA
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    mU6

    Available Since

    Oct. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. EK0211 CMV-TO-BFPmut-stop-t17d9b (FLP-IN)

    Plasmid

    #191139

    Purpose

    Expression of Trigger 2 in 3' UTR of an inactive BFP in mammalian cells. Used as second input in OR, AND gates

    Depositor

    Xiaojing Gao

    Article

    Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

    Insert

    t17d9b
    

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    CMV-TO

    Available Since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pAAV-VTKS2-Dreadd-hM3Dq-HA-P2A-tBFP

    Plasmid

    #178707

    Purpose

    AAV vector for Cre-dependent transgene expression of Dreadd-hM3Dq-HA-P2A-tBFP in neurons under the control of the hSyn1 promoter (Kugler et al 2003)

    Depositor

    Jordane Dimidschstein

    Article

    Pouchelon et al Cell Rep Methods. 2022 May 26;2(6):10022

    Insert

    Dreadd-hM3Dq-HA-P2A-tBFP
    

    Use

    AAV

    Promoter

    hSyn

    Available Since

    May 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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36. pSCGB3A2_HL-P2A-TagBFP-PGK-NeoR-SCGB3A2_HR

    Plasmid

    #126697

    Purpose

    donor vector for targeting a 2A peptide followed by blue fluorescent reporter to the human SCGB3A2 locus at the end of the endogenous coding sequence

    Depositor

    Darrell Kotton

    Article

    McCauley et al Stem Cell Reports. 2018 May 8;10(5):1579

    Insert

    TagBFP
    

    Expression

    Mammalian

    Available Since

    July 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. LV anti-FITC-LaG16-Y186F-T2A-BFP

    Plasmid

    #194931

    Purpose

    LV construct encoding Y186F mutant sNRR-based SynNotch receptor

    Depositor

    John Ngo

    Article

    Sloas et al Nat Biotechnol. 2023 Jan 16. doi: 10.103

    Insert

    antiFITC-LaG16-sNRR(Y186F)-SynNotch-Gal4-VP64-T2A-BFP
    

    Use

    Lentiviral and Synthetic Biology

    Available Since

    March 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pCVL SFFV HA.NLS.I-AniIY2(reo).T2A.TagBFP

    Plasmid

    #31477

    Depositor

    Andrew Scharenberg

    Article

    Certo et al Nat Methods. 2011 Jul 10;8(8):671-6. doi

    Insert

    SFFV HA NLS Y2 T2A mTagBFP
    

    Use

    Lentiviral

    Tags

    HA and NLS

    Mutation

    F13Y + S111Y on I-AniI = Y2 "reo" (res…

    Available Since

    Aug. 22, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. JDW 827 (pCS2-V5-mTagBFP2-hsKRAS4A-G12D)

    Plasmid

    #156409

    Purpose

    pCS2 CMV expression vector driving V5-tagged mTagBFP2 fused human KRAS4A-G12D

    Depositor

    Joshua Wythe

    Article

    Fish et al Circ Res. 2020 Jun 17. doi: 10.1161/CIRC

    Insert

    human mutant KRAS4A-G12D (KRAS Human)
    

    Tags

    mTagBFP2

    Expression

    Mammalian

    Mutation

    mutant KRAS4A-G12D

    Promoter

    CMV

    Available Since

    Aug. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pcDNA3.1-TagBFP-P2A-Pre-SPHERE-SF-iGluSnFR

    Plasmid

    #187089

    Purpose

    Glutamate sensor for localization to membrane contatct

    Depositor

    Kotaro Oka

    Article

    Shindo et al bioRxiv 2022

    Insert

    Pre-SPHERE-SF-iGluSnFR
    

    Tags

    TagBFP-P2A

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge