163,682 results

1. pIRES-Puro-MGMT

   Plasmid

   #187654

   Purpose

   Expresses hMGMT together with the puromycin resistance gene via an IRES.

   Depositor

   Robert Sobol

   Article

   Li et al Cancers (Basel). 2022 Jul 22;14(15). pii

   Insert

   MGMT (MGMT Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Sept. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBBR1_DVK_AE(J23100-BCD2-E0040m-B0015)

   Plasmid

   #197660

   Purpose

   Expresses GFP under the control of J23100 and BCD2

   Depositor

   Eric Young

   Article

   Keating et al ACS Synth Biol. 2023 Jun 9. doi: 10.1021

   Insert

   GFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   June 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pACE-GIRK2-S

   Plasmid

   #172426

   Purpose

   Expression of full-length, human GIRK2 with C-terminal StrepTag II

   Depositor

   Arthur Laganowsky

   Article

   Qiao et al Biochemistry. 2021 Dec 21;60(50):3813-38

   Insert

   GIRK2 (KCNJ6 Human)
   

   Use

   Tags

   Streptag II

   Expression

   Insect

   Mutation

   Promoter

   Available Since

   Oct. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. mRuby2-ZO1-C-14

   Plasmid

   #55916

   Purpose

   Localization: Tight Junctions, Excitation: 559, Emission: 600

   Depositor

   Michael Davidson

   Article

   Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

   Insert

   ZO1 (TJP1 Human)
   

   Use

   Tags

   mRuby2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   March 10, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

5. FASTHDR-Cterm-HaloTag-Puromycin

   Plasmid

   #167208

   Purpose

   Plasmid to clone recombination arms to create homologous recombination donor vector for C-terminal gene tagging with HaloTag and selection with Puromycin

   Depositor

   Oscar Perez

   Article

   Perez-Leal et al CRISPR J. 2021 Nov 30. doi: 10.1089/cris

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   HaloTag

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Dec. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHTN-HaloTag_BCL11A:1-773

   Plasmid

   #211014

   Purpose

   Mammalian expression of full-length human BCL11A. N-terminal HaloTag.

   Depositor

   Cheryl Arrowsmith

   Article

   Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

   Insert

   BCL11A:M1-F773 (BCL11A Human)
   

   Use

   Tags

   HaloTag

   Expression

   Mammalian

   Mutation

   isoform 2

   Promoter

   CMV

   Available Since

   Feb. 21, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pUC19/mChe-PM

   Plasmid

   #183168

   Purpose

   Construct for transient expression of plasma membrane-targeted mCherry in plants.

   Depositor

   Qinlong Zhu, Yao-Guang Liu

   Article

   Han et al Plant Biotechnol J. 2022 Feb 18. doi: 10

   Insert

   mCherry-PM
   

   Use

   Tags

   OsRac3, O. sativa rac-like GTP-binding protein 3

   Expression

   Plant

   Mutation

   Promoter

   Available Since

   Aug. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCS2-nCas9n

   Plasmid

   #47929

   Purpose

   expression of an optimized Cas9 for genome-editing in zebrafish

   Depositor

   Wenbiao Chen

   Article

   Jao et al Proc Natl Acad Sci U S A. 2013 Aug 5.

   Insert

   nls-zcas9-nls
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Aug. 29, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. pScPPX2

   Plasmid

   #112877

   Purpose

   Expresses His-tagged yeast exopolyphosphatase for purification from E. coli

   Depositor

   Ursula Jakob, Michael Gray

   Article

   Gray et al Mol Cell. 2014 Mar 6;53(5):689-99. doi:

   Insert

   PPX1 (PPX1 Budding Yeast)
   

   Use

   Tags

   6X His tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Aug. 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGEX-4T MDM2 WT

    Plasmid

    #16237

    Depositor

    Mien-Chie Hung

    Article

    Zhou et al Nat Cell Biol. 2001 Nov . 3(11):973-82.

    Insert

    MDM2 (MDM2 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Nov. 30, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. p11L2w

    Plasmid

    #37331

    Depositor

    John Schiller

    Article

    Kieback et al Virology. 2006 Feb 5;345(1):199-208. Epu

    Insert

    HPV11 L2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    July 8, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. mCherry-PCNA

    Plasmid

    #215117

    Purpose

    Expresses mCherry-PCNA in mammalian cells from a lentiviral vector.

    Depositor

    Michele Pagano

    Article

    Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

    Insert

    PCNA (PCNA Human)
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. His6tev CBP HAT (1324-1700)

    Plasmid

    #99340

    Purpose

    bacterial expression of His tagged mouse CBP HAT domain (1324-1700)

    Depositor

    Peter Wright

    Article

    Park et al Proc Natl Acad Sci U S A. 2017 Jun 19. p

    Insert

    mouse CBP HAT domain (1324-1700) (Crebbp Mouse)
    

    Use

    Tags

    His6tev

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Sept. 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLVX-EF1a-EGFP-RAB11B-IRES-Puromycin

    Plasmid

    #134860

    Purpose

    Expresses EGFP-tagged recycling endosome marker

    Depositor

    David Andrews

    Article

    Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

    Insert

    RAB11B (RAB11B Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    March 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAXY0002

    Plasmid

    #179835

    Purpose

    binary plant vector expressing eYGFPuv and GUS, both driven by a 35S promoter

    Depositor

    Xiaohan Yang

    Article

    Yuan et al Hortic Res. 2021 Nov 1;8(1):234. doi: 10

    Type

    Empty backbone

    Use

    Binary vector for plant transformation

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    Nov. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA3.1 Neo(+) hSUMF1

    Plasmid

    #24386

    Depositor

    Carolyn Bertozzi

    Article

    Wu et al Proc Natl Acad Sci U S A. 2009 Mar 3. 10

    Insert

    hSUMF1 (SUMF1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 2, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. OMM-SPLICSs-nFAST

    Plasmid

    #210430

    Purpose

    Expresses outer mitochondria-targeted split-NFAST for Short-range ER-Mito Contact sites

    Depositor

    Tito Calì

    Insert

    OMM-SPLICSs-nFAST
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pRRL-TetO-Pax7-IRES-EGFP

    Plasmid

    #109372

    Purpose

    Expresses Dox-inducible Pax7 together with a GFP reporter

    Depositor

    Nenad Bursac

    Article

    Rao et al Nat Commun. 2018 Jan 9;9(1):126. doi: 10

    Insert

    Pax7
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCFJ782 - HygroR

    Plasmid

    #190933

    Purpose

    Hygromycin resistance co-injection marker

    Depositor

    Christian Froekjaer-Jensen

    Article

    El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

    Insert

    Hygromycin resistance gene
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    rps-0

    Available Since

    Nov. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. RCIscript-GoldyTALEN

    Plasmid

    #38142

    Purpose

    destination vector for the Golden Gate TALEN kit, for in vitro synthesis of TALEN mRNAs

    Depositor

    Stephen Ekker, Daniel Carlson

    Article

    Carlson et al Proc Natl Acad Sci U S A. 2012 Oct 1.

    Insert

    GoldyTALEN
    

    Use

    Mrna transcription vector

    Tags

    AcV5 and FokI homodimer

    Expression

    Mutation

    Truncate at N and C terminous

    Promoter

    T3

    Available Since

    Sept. 20, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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21. pSPIH6

    Plasmid

    #190676

    Purpose

    E. coli plasmid for single step cloning of SUMO-peptide-intein (SPI) fusion proteins (contains SUMO and His-tagged intein)

    Depositor

    John Vederas

    Article

    Lamer et al BMC Biotechnol. 2023 Apr 5;23(1):11. doi

    Inserts

    Small ubiquitin-like modifier
    Mxe GyrA intein with C-terminal chitin binding domain and His tag
    

    Use

    Tags

    Chitin binding domain, His tag, and N-terminal Hi…

    Expression

    Bacterial

    Mutation

    Added C-terminal his tag and None

    Promoter

    T7

    Available Since

    Feb. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. 4X PRE TK luc

    Plasmid

    #206159

    Purpose

    Luciferase reporter construct containing 4 consensus PGR binding sites upstream of a minimal TK promoter, derived from Addgene #11350

    Depositor

    Renee van Amerongen

    Article

    Aarts et al J Mammary Gland Biol Neoplasia. 2023 Nov

    Insert

    4X PRE TK luciferase
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. TRUPATH Triple Gai1

    Plasmid

    #196048

    Purpose

    Encodes a G alpha subunit (GNAl1) with RLuc8, a G gamma subunit (GNG9) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins

    Depositor

    Justin English

    Article

    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

    Inserts

    GAlphai1-RLuc8 (GNAI1 Human)
    GGamma9-GFP2
    GBeta3 (GNB3 Human)
    

    Use

    Luciferase

    Tags

    GFP2 and GSAG linker

    Expression

    Mammalian

    Mutation

    RLuc8 and flanking SGGGGS linkers have been inser…

    Promoter

    CMV

    Available Since

    March 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pYSEAP

    Plasmid

    #37326

    Depositor

    John Schiller

    Article

    Pastrana et al Virology. 2004 Apr 10;321(2):205-16.

    Insert

    SEAP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    July 23, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pDONR223-SRC

    Plasmid

    #23934

    Depositor

    William Hahn, David Root

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    SRC (SRC Human)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

26. Lenti_MS2_P65_HSF1_2A_puro

    Plasmid

    #118699

    Purpose

    To express MS2-P65-HSF1

    Depositor

    Christopher Vakoc

    Article

    Lu et al Cancer Cell. 2018 Dec 10;34(6):970-981.e

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pcDNA3-mitoExRai-AKAR2

    Plasmid

    #161757

    Purpose

    ExRai-AKAR2 targeted to the outer mitochondrial membrane.

    Depositor

    Jin Zhang

    Article

    Zhang et al Nat Chem Biol. 2020 Sep 28. pii: 10.1038

    Insert

    DAKAP-ExRai-AKAR2
    

    Use

    Tags

    N-terminal 30 amino acids from DAKAP1

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Nov. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. plix403-V5-N-mEGFP

    Plasmid

    #223957

    Purpose

    Gateway cloning of genes of interest with an N-terminal V5-mEGFP fusion; generation of stable cells with doxycycline inducible expression

    Depositor

    Karla Feijs, Roko Žaja

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    V5-mEGFP

    Expression

    Mutation

    Promoter

    Available Since

    Oct. 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pLENTI_RPS3_Keima

    Plasmid

    #127140

    Depositor

    Thomas Tuschl

    Article

    Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

    Insert

    RPS3_Keima (RPS3 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pAAV-RHO-IntC-dCas9C-VPR-polyA

    Plasmid

    #166692

    Purpose

    Express C-terminal part of split dCas9-VPR

    Depositor

    Stylianos Michalakis

    Article

    Bohm et al Sci Adv. 2020 Aug 19;6(34):eaba5614. doi

    Insert

    IntC-dCas9C-VPR
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    short human rhodopsin promoter

    Available Since

    Dec. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge