165,947 results

1. pRN11

   Plasmid

   #84455

   Purpose

   shuttle plasmid for sarA P1-mCherry expression

   Depositor

   Reindert Nijland

   Article

   de Jong et al Sci Rep. 2017 Mar 7;7:43889. doi: 10.103

   Insert

   mCherry
   

   Use

   Shuttle vector e.coli-s.aureus, expression of sgf…

   Available Since

   Feb. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCas9-Geminin

   Plasmid

   #109401

   Purpose

   Expresses Cas9 fusion to Geminin degron domain

   Depositor

   Jean-Paul Concordet

   Article

   Charpentier et al Nat Commun. 2018 Mar 19;9(1):1133. doi:

   Insert

   Geminin (GMNN Human)
   

   Mutation

   Geminin degron fragment

   Available Since

   May 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA/Myc TCF4

   Plasmid

   #16512

   Depositor

   Bert Vogelstein

   Article

   Korinek et al Science. 1997 Mar 21. 275(5307):1784-7.

   Insert

   TCF4 (TCF7L2 Human)
   

   Tags

   Myc

   Expression

   Mammalian

   Available Since

   May 16, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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4. TXNRD1

   Plasmid

   #38863

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   TXNRD1 (Txnrd1 Human)
   

   Tags

   His Tev

   Expression

   Bacterial

   Available Since

   March 18, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHACK-GAL4>QF2

   Plasmid

   #80275

   Purpose

   GAL4->QF2 HACK construct. Contains 5' and 3' GAL4 homology arms and gRNAs.

   Depositor

   Christopher Potter

   Article

   Lin et al Genetics. 2016 Jun 22. pii: genetics.116

   Insert

   QF2
   

   Expression

   Insect

   Available Since

   Aug. 2, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSFFV_sfCherry2_TagBFP

   Plasmid

   #83031

   Purpose

   Expresses full length sfCherry2 and TagBFP fusion in mammalian cells

   Depositor

   Bo Huang

   Article

   Feng et al Nat Commun. 2017 Aug 29;8(1):370. doi: 1

   Insert

   sfCherry+full length_TagBFP
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   3 mutations in sfCherry full length: E118Q, T128I…

   Promoter

   SFFV

   Available Since

   Jan. 16, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. mCh-Sec61 beta

   Plasmid

   #49155

   Purpose

   expression of Sec61 beta fused to mCherry, used as a general ER marker

   Depositor

   Gia Voeltz

   Article

   Zurek et al Traffic. 2011 Jan;12(1):28-41. doi: 10.1

   Insert

   Sec61 beta (SEC61B Human)
   

   Tags

   mCherry*

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 20, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. pFH2.22_mCherry-MS2x8

   Plasmid

   #205537

   Purpose

   Express mCherry-MS2x8 (export tag consisting of 8 tandem repeats of MS2 stem-loop aptamer)

   Depositor

   Michael Elowitz

   Article

   Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

   Insert

   mCherry
   

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   CMV

   Available Since

   Sept. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. ND4-DdCBE- left side TALE

   Plasmid

   #157844

   Purpose

   expresses left-side TALE–DddAtox half ND4-DdCBE in mammalian cells

   Depositor

   David Liu

   Article

   Mok et al Nature. 2020 Jul 8. pii: 10.1038/s41586-

   Insert

   SOD2 MTS–3x-HA–ND4 left tale–G1397 DddA-C–UGI–SOD2 3'UTR
   

   Expression

   Mammalian

   Available Since

   Aug. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCDH-EF1-Nluc-P2A-copGFP-T2A-Puro

    Plasmid

    #73022

    Purpose

    Luminescent and fluorescent dual reporter

    Depositor

    Kazuhiro Oka

    Insert

    Nluc-P2A-copGFP-T2A-Puro
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF-1

    Available Since

    Jan. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1-HaloAKAR2.1-T2A-EGFP

    Plasmid

    #216489

    Purpose

    Expression of chemigenetic PKA biosensor (medium basal brightness, high dynamic range) and EGFP transfection marker in mammalian cells

    Depositor

    Jin Zhang

    Article

    Frei et al Nat Biotechnol. 2025 Apr 21. doi: 10.103

    Insert

    HaloAKAR2.1
    

    Tags

    T2A-EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSGAb-km

    Plasmid

    #121999

    Purpose

    A sgRNA expression plasmid for genome editing in Acinetobacter baumannii

    Depositor

    Quanjiang Ji

    Article

    Wang et al Cell Chem Biol. 2019 Sep 17. pii: S2451-

    Insert

    none
    

    Use

    CRISPR

    Available Since

    Sept. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. EGFP-Actin-7

    Plasmid

    #56421

    Purpose

    Localization: Actin, Excitation: 488, Emission: 507

    Depositor

    Michael Davidson

    Article

    Rizzo et al Cold Spring Harb Protoc. 2009 Dec;2009(1

    Insert

    Actin (ACTB Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 23, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pcDNA3-ExRai-CKAR

    Plasmid

    #118409

    Purpose

    cpGFP-based excitation-ratiometric biosensor for monitoring Protein Kinase C activity.

    Depositor

    Jin Zhang

    Article

    Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

    Insert

    ExRai-CKAR
    

    Tags

    6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. GHRHR-Tango

    Plasmid

    #66292

    Purpose

    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

    Depositor

    Bryan Roth

    Article

    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

    Insert

    GHRHR (GHRHR Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

16. pH1R-mCherry-N1

    Plasmid

    #84325

    Purpose

    Histamine-1 receptor tagged with mCherry

    Depositor

    Dorus Gadella

    Article

    van Unen et al Mol Pharmacol. 2016 Sep;90(3):162-76. do

    Insert

    H1R (HRH1 Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLVX-Puro-hGSDME

    Plasmid

    #154876

    Purpose

    Expresses hGSDME in mammalian cells

    Depositor

    Judy Lieberman

    Article

    Zhang et al Nature. 2020 Mar;579(7799):415-420. doi:

    Insert

    GSDME (GSDME Human)
    

    Use

    Lentiviral

    Promoter

    CMV

    Available Since

    July 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pShHELIX(Cas12k-TnsC)-sgRNA_entry (CJT113)

    Plasmid

    #181792

    Purpose

    Expresses 3-component ShHELIX containing a Cas12k-TnsC fusion. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).

    Depositor

    Benjamin Kleinstiver

    Article

    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    nAniI-ShTnsB, ShTniQ, ShCas12k-ShTnsC
    

    Expression

    Bacterial

    Mutation

    nAniI = K227M, F80K, L232K

    Promoter

    Lac and J23119

    Available Since

    March 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pKDsgRNA-FRT

    Plasmid

    #113398

    Purpose

    expresses gRNA for Cas9 FRT targetting

    Depositor

    Jan Michiels

    Article

    Swings et al Nat Commun. 2018 Jun 8;9(1):2231. doi: 1

    Insert

    exo, beta, gam, sgRNA-FRT
    

    Use

    CRISPR

    Expression

    Bacterial

    Available Since

    Aug. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. ND4-DdCBE-right side TALE

    Plasmid

    #157843

    Purpose

    expresses right-side TALE–DddAtox half ND4-DdCBE in mammalian cells

    Depositor

    David Liu

    Article

    Mok et al Nature. 2020 Jul 8. pii: 10.1038/s41586-

    Insert

    COX8A MTS–3xFLAG–ND4 right TALE–G1397 DddA-N–UGI–ATP5B 3'UTR
    

    Expression

    Mammalian

    Available Since

    Aug. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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21. pBS SK mCherryROSAegfp

    Plasmid

    #54322

    Purpose

    Encodes mCherry fused with an out-of-frame EGFP through a linker containing a target for TALEN/CRISPR. Can be used as a surrogate target to test the specificity of TALENs and CRISPR.

    Depositor

    Silvestro Conticello

    Article

    Niccheri et al Cell Mol Life Sci. 2017 Apr 18. doi: 10.

    Insert

    mCherry and EGFP
    

    Use

    CRISPR, Mouse Targeting, and TALEN

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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22. CABE-T1.17

    Plasmid

    #193260

    Purpose

    Expression of CABE-T1.17 base editor in mammalian cells, CMV promoter

    Depositor

    Nicole Gaudelli

    Article

    Lam et al Nat Biotechnol. 2023 Jan 9. doi: 10.1038

    Insert

    CABE-T1.17
    

    Expression

    Mammalian

    Mutation

    D10A (Cas9), TadA mutations within

    Promoter

    CMV

    Available Since

    Jan. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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23. pCAG-Synapto-pHluorin

    Plasmid

    #168510

    Purpose

    Expression of Synapto-pHluorin for optical monitoring of presynaptic release in neurons.

    Depositor

    Franck Polleux

    Article

    Kwon et al PLoS Biol. 2016 Jul 18;14(7):e1002516. d

    Insert

    Synaptophysin-pHluorin (Syp Mouse)
    

    Tags

    pHluorin

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    April 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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24. pCAG_smFP FLAG

    Plasmid

    #59756

    Purpose

    encodes multiple copies of an epitope tag FLAG

    Depositor

    Loren Looger

    Article

    Viswanathan et al Nat Methods. 2015 Jun;12(6):568-76. doi:

    Insert

    smFP _FLAG
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Oct. 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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25. pNC-Cam1304-RNAi

    Plasmid

    #193399

    Purpose

    Nimble cloning-compatible vector for plant RNAi

    Depositor

    Pu Yan

    Article

    Yan et al Plant Commun. 2023 Mar 13;4(2):100471. d

    Type

    Empty backbone

    Expression

    Plant

    Available Since

    Dec. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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26. pET22-HT/plsA

    Plasmid

    #73625

    Purpose

    Produces Escherichia coli adenylate kinase (PlsA), N-terminal His6 tag

    Depositor

    T. J. Kappock, James Williamson

    Article

    Schultheisz et al ACS Chem Biol. 2008 Aug 15;3(8):499-511.

    Insert

    adenylate kinase (adk Escherichia coli str. K-12 substr. MG1655)
    

    Tags

    His6

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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27. MSCV-pU6-(BbsI)-CcdB-(BbsI)-Pgk-Puro-T2A-BFP

    Plasmid

    #86457

    Purpose

    Mouse stem cell retroviral vector including a puromycin resistance and BFP gene. sgRNA targets can be cloned in between the BbsI sites

    Depositor

    Ralf Kuehn

    Article

    Chu et al Proc Natl Acad Sci U S A. 2016 Nov 1;113

    Insert

    BFP
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    Pgk

    Available Since

    Feb. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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28. Tag5Amyc-GSK3b KD

    Plasmid

    #16262

    Depositor

    Mien-Chie Hung

    Article

    Zhou et al Nat Cell Biol. 2004 Oct . 6(10):931-40.

    Insert

    GSK3 beta (GSK3B Human)
    

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Kinase dead: K85A

    Available Since

    Nov. 30, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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29. AiP14532-pAAV-DLX2.0-minBG-FlpO-WPRE3-BGHpA (Alias: CN4532)

    Plasmid

    #222939

    Purpose

    FlpO recombinase expression from the DLX2.0 enhancer

    Depositor

    Jonathan Ting

    Article

    Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

    Insert

    FlpO (flp Synthetic)
    

    Use

    AAV

    Available Since

    Sept. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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30. pLenti-EML4-ALK variant 3a

    Plasmid

    #183829

    Purpose

    Express EML4-ALK fusion variant 3a (E6a;A20) in mammalian cells

    Depositor

    Aaron Hata

    Article

    Yoda et al Cancer Discov. 2018 Jun;8(6):714-729. do

    Inserts

    ALK Receptor Tyrosine Kinase (ALK Human)
    EMAP Like 4 (EML4 Human)
    

    Use

    Lentiviral

    Tags

    The fusion protein of EML4 exon 1-6a and ALK exon…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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