Skip to main content
Addgene
Showing: 9151 - 9180 of 157954 results

  6. pCMV_NESPylRS(AF)_hU6tRNAPyl

    Plasmid
    #223508
    Purpose
    PylRS (AF)/tRNA Pyl orthogonal pair for genetic code expansion that allows site-specific incorporation of noncanonical amino-acids into a POI using the Amber stop codon
    Depositor
    Edward Lemke
    Article
    Nikic et al Angew Chem Int Ed Engl. 2016 Dec 23;55(5
    Inserts
    NES Pyrrolysyl-tRNA Synthetase (AF) (pylS Methanosarcina mazei)
    tRNA Pyl
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Y306A; Y384F
    Promoter
    CVM, SP6 and U6
    Available since
    Oct. 7, 2024
    Availability
    Academic Institutions and Nonprofits only
    223508-plasmid-map-sequence-id-445174

    Enlarge

  13. pSAM_AraC

    Plasmid
    #91569
    Purpose
    Mariner transposon mutagenesis & sequencing vector - Transposon contains KanR and Illumina sequencing adapters. Arabinose promoter expresses transposase. Ampicillin resistance on backbone.
    Depositor
    Harry Mobley
    Article
    Armbruster et al PLoS Pathog. 2017 Jun 14;13(6):e1006434.
    Insert
    AraC/PBAD
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    91569-plasmid-map-sequence-id-181631

    Enlarge

  23. pB-rtTA

    Plasmid
    #126034
    Purpose
    PiggyBac cargo vector expressing rtTA. Also confers resistance to G418.
    Depositor
    Mauro Calabrese
    Article
    Kirk et al Nat Genet. 2018 Oct;50(10):1474-1482. do
    Insert
    rtTA3-IRES-Neo cassette cloned from Addgene Plasmid #25735
    Use
    Piggybac
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    hUbiC
    Available since
    June 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    126034-plasmid-map-sequence-id-245792

    Enlarge

  27. BIC-Gag-CAS9

    Plasmid
    #119942
    Purpose
    encodes a GAG (F-MLV)-CAS9(sp) fusion. Allows the production of GAG-CAS9 Virus like particles from producer cells in association with over expressed gRNA(s) and appropriate envelopes.
    Depositor
    Emiliano Ricci, Théophile Ohlmann, Philippe Mangeot
    Article
    Mangeot et al Nat Commun. 2019 Jan 3;10(1):45. doi: 10
    Insert
    GAG FMLV fused to spCAS9
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    hCMV
    Available since
    April 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    119942-plasmid-map-sequence-id-235075

    Enlarge

  28. pUCC001

    Plasmid
    #124451
    Purpose
    Multi-species CRISPR/Cas9 coexpression system, optimized for Golden Gate cloning of gRNA targets
    Depositor
    John Morrissey
    Article
    Rajkumar et al Front Bioeng Biotechnol. 2019 May 7;7:97
    Insert
    HH-BsaI
    Use
    Tags
    Expression
    Yeast
    Mutation
    gRNA expression cassette from pUDP002 modified to…
    Promoter
    TDH3 promoter from S.cerevisiae (already present …
    Available since
    Sept. 26, 2019
    Availability
    Academic Institutions and Nonprofits only
    124451-plasmid-map-sequence-id-257763

    Enlarge

Showing: 9151 - 9180 of 157954 results