159,557 results

1. pExpreS2-1-C3-PA-10H-SIII

   Plasmid

   #175443

   Purpose

   HiFi-compatible destination vector for secreted overexpression of 3C-cleavable, C-terminal Protein A-10His-TwinStrep tagged proteins with ExpreS2 Platform

   Depositor

   Wyatt Yue

   Article

   Coker et al Front Bioeng Biotechnol. 2022 May 5;10:8

   Type

   Empty backbone

   Use

   Tags

   3C-Protein A-10His-TwinStrep and BiP Secretion Pe…

   Expression

   Insect

   Mutation

   Promoter

   Fused Actin-HSP70

   Available since

   April 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET-6xHis-(pos16)GFP

   Plasmid

   #89249

   Purpose

   Expresses GFP with +16 theoretical charge in E.Coli

   Depositor

   David Liu

   Article

   Thompson et al Chem Biol. 2012 Jul 27;19(7):831-43. doi

   Insert

   His6-(pos16)-GFP
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCW57.1-Eomes-3xT7

   Plasmid

   #200888

   Purpose

   Inducible lentiviral expression of Eomes

   Depositor

   Kai Ge

   Article

   Xie et al Nat Genet. 2023 Apr 3. doi: 10.1038/s415

   Insert

   Eomes (Eomes Mouse)
   

   Use

   Lentiviral; Doxycycline inducible

   Tags

   3xT7

   Expression

   Mammalian

   Mutation

   Promoter

   TRE promoter, Tet ON

   Available since

   June 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDZ416 (24PP7SL loxP-Kan-loxP)

   Plasmid

   #45163

   Depositor

   Daniel Zenklusen, Robert Singer

   Article

   Hocine et al Nat Methods. 2013 Feb;10(2):119-21. doi:

   Insert

   24xPP7 Stem Loop Cassette
   

   Use

   Cre/Lox

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 29, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. pKDsgRNA-ack

   Plasmid

   #62654

   Purpose

   Arabinose inducible lambda red and anhydrotetracycline inducible sgRNA expression

   Depositor

   Kristala Prather

   Article

   Reisch et al Sci Rep. 2015 Oct 14;5:15096. doi: 10.10

   Insert

   exo, beta, gam, sgRNA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 19, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. PB-CuO-V5 CASC3 siRes1+2 f.l. WT

   Plasmid

   #158540

   Purpose

   For PiggyBac-mediated integration of V5-tagged siRNA-resistant full-length CASC3

   Depositor

   Niels Gehring

   Article

   Gerbracht et al Nucleic Acids Res. 2020 Jul 4. pii: 5867

   Insert

   CASC3; BTZ; MLN51 (CASC3 Human)
   

   Use

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pWUR790

   Plasmid

   #101723

   Purpose

   Synthetic codon optimized P. furiosus ago with N-terminal Strep(II)-tag in pCDF-1b vector, expression vector for PfAgo

   Depositor

   John van der Oost

   Article

   Swarts et al Nucleic Acids Res. 2015 May 26;43(10):51

   Insert

   PfAgo
   

   Use

   Tags

   Strep(II)-tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7 Promotor

   Available since

   Nov. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTECH-MbPylRS(IPYE)

   Plasmid

   #104072

   Purpose

   Expression plasmid encoding MbPylRS(IPYE) and pylT

   Depositor

   David Liu

   Article

   Bryson et al Nat Chem Biol. 2017 Oct 16. doi: 10.1038

   Insert

   Plpp MbPylRS(IPYE); PproK pyl
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Contains activating mutations V31I, T56P, H62Y, A…

   Promoter

   Plpp and PproK

   Available since

   Dec. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBAC-lacZ

   Plasmid

   #13422

   Depositor

   Keith Joung

   Article

   Wright et al Nat Protoc. 2006;1(3):1637-52. doi: 10.1

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Cre/Lox

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 8, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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10. PKD1 gRNA (BRDN0001148399)

    Plasmid

    #76842

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PKD1

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    PKD1 (PKD1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-nEF Coff/Fon DREADD Gq-mCherry

    Plasmid

    #183534

    Purpose

    INTRSECT Chemogenetics - expresses DREADD Gq-mCherry in cells expressing Flp but not Cre

    Depositor

    Karl Deisseroth

    Insert

    DREADD Gq-mCherry
    

    Use

    AAV

    Tags

    Expression

    Mutation

    None

    Promoter

    nEF

    Available since

    May 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pKM461

    Plasmid

    #108320

    Purpose

    For performing ORBIT - Expresses Che9c phage RecT and Bxb1 phage Integrase, and contains SacB

    Depositor

    Kenan Murphy

    Article

    Murphy et al MBio. 2018 Dec 11;9(6). pii: mBio.01467-

    Inserts

    Che9C RecT
    Bxb1 Integrase
    Tet Repressor
    SacRB
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. GNRHR-Tango

    Plasmid

    #66297

    Purpose

    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

    Depositor

    Bryan Roth

    Article

    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

    Insert

    GNRHR (GNRHR Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

14. mStrawberry-N1

    Plasmid

    #54644

    Purpose

    Localization: N1 Cloning Vector, Excitation: 574, Emission: 596

    Depositor

    Nathan Shaner, Michael Davidson, Roger Tsien

    Article

    Shaner et al Nat Biotechnol. 2004 Dec;22(12):1567-72.

    Type

    Empty backbone

    Use

    Tags

    mStrawberry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAAV-hSyn-mCherry-sesRNA-smFlag-tTA2-W3SL

    Plasmid

    #192066

    Purpose

    Expression of mCherry, sesRNA in mammalian cells, with smFlag and tTA2 as efRNA.

    Depositor

    Josh Huang

    Article

    Qian et al Nature. 2022 Oct;610(7933):713-721. doi:

    Insert

    sesRNA for Mouse Ctip2
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn

    Available since

    Jan. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. mRFP1-N1

    Plasmid

    #54635

    Purpose

    Localization: N1 Cloning Vector, Excitation: 584, Emission: 607

    Depositor

    Robert Campbell, Michael Davidson, Roger Tsien

    Article

    Campbell et al Proc Natl Acad Sci U S A. 2002 Jun 11;99

    Type

    Empty backbone

    Use

    Tags

    mRFP1

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA3.1(+)-Lifeact-(GGS)X6-HaloTag C58

    Plasmid

    #171435

    Purpose

    Expression of Lifeact-(GGS)X6-HaloTag C58 in mammalian cells

    Depositor

    Yujie Sun

    Article

    Shao et al Commun Biol. 2021 Mar 19;4(1):378. doi:

    Insert

    Lifeact-(GGS)X6-HaloTag C58
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. Klf2-t2A-mCherry

    Plasmid

    #127539

    Purpose

    Expresses mouse Klf2 and mCherry via t2A linker.

    Depositor

    Jennifer Mitchell

    Article

    Dhaliwal et al Genes Dev. 2019 Jun 20. pii: gad.324319.

    Insert

    Klf2 (Klf2 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pYFP-ErbB2

    Plasmid

    #66948

    Purpose

    Mammalian expression of rat ErbB2 tagged with YFP

    Depositor

    Mads Lerdrup, Bo van_Deurs

    Article

    Lerdrup et al Mol Biol Cell. 2007 Sep;18(9):3656-66. E

    Insert

    ErbB2 (Erbb2 Rat)
    

    Use

    Tags

    6xHis, V5 Tag, and YFP

    Expression

    Mammalian

    Mutation

    (See depositor comment below on mutations A24T an…

    Promoter

    CMV

    Available since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

20. CD44P pGL3

    Plasmid

    #19122

    Depositor

    Bob Weinberg

    Article

    Godar et al Cell. 2008 Jul 11. 134(1):62-73.

    Insert

    CD44 promoter (CD44 Human)
    

    Use

    Non viral cloning vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 3, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. 1136- Desmoplakin-GFP

    Plasmid

    #32227

    Depositor

    Kathleen Green

    Article

    Godsel et al J Cell Biol. 2005 Dec 19;171(6):1045-59.

    Insert

    Desmoplakin (DSP Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 8, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. PKD1 gRNA (BRDN0001149544)

    Plasmid

    #76841

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PKD1

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    PKD1 (PKD1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. hyeA3A-BE4max

    Plasmid

    #157944

    Purpose

    Mammalian expression of hyeA3A-BE4max

    Depositor

    Dali Li

    Article

    Zhang et al Nat Cell Biol. 2020 Jun;22(6):740-750. d

    Insert

    hyeA3A-BE4max
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    N57G

    Promoter

    Available since

    Jan. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. CL20_mEGFP_NUP98_KDM5A

    Plasmid

    #205872

    Purpose

    Express mEGFP-tagged fusion protein, NUP98_KDM5A from patient-derived sequence

    Depositor

    Richard Kriwacki

    Article

    Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

    Insert

    NUP98_KDM5A (NUP98, KDM5A Human)
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pDY0663 ACTB atgRNA with v2 scaffold

    Plasmid

    #179108

    Purpose

    ACTB N-term PBS 13 RT 29 Bxb1 AttB 46 atgRNA with v2 scaffold

    Depositor

    Jonathan Gootenberg, Omar Abudayyeh

    Article

    Yarnall et al Nat Biotechnol. 2022 Nov 24. doi: 10.103

    Insert

    ACTB N-term PBS 13 RT 29 AttB 46 atgRNA with v2 scaffold
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pHM6-alphasynuclein-WT

    Plasmid

    #40824

    Depositor

    David Rubinsztein

    Article

    Furlong et al Biochem J. 2000 Mar 15;346 Pt 3:577-81.

    Insert

    SNCA (SNCA Human)
    

    Use

    Tags

    6x His and HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 8, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pSBbi-GN

    Plasmid

    #60517

    Purpose

    SB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: GFP and neomycin resistance gene

    Depositor

    Eric Kowarz

    Article

    Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b

    Type

    Empty backbone

    Use

    Transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a/RPBSA

    Available since

    Feb. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pCAG_smFP V5

    Plasmid

    #59758

    Purpose

    encodes multiple copies of an epitope tag V5

    Depositor

    Loren Looger

    Article

    Viswanathan et al Nat Methods. 2015 Jun;12(6):568-76. doi:

    Insert

    smFP _V5
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Sept. 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. 7MEQ

    Plasmid

    #176412

    Purpose

    Baculovirus expression for structure determination. May not contain entire coding region of gene.

    Depositor

    Cheryl Arrowsmith

    Insert

    TMPRSS2 – transmembrane serine protease 2 (TMPRSS2 Human)
    

    Use

    Baculovirus expresssion

    Tags

    8 x His Tag and Honeybee melittin signal

    Expression

    Mutation

    etmprss2_S251D/R252D/Q253D/S254D/R255K

    Promoter

    Polyhedrin

    Available since

    Oct. 21, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

30. C1QBP-bio-His

    Plasmid

    #53330

    Purpose

    Expresses full-length Complement component 1 Q subcomponent-binding protein ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    C1QBP (C1QBP Human)
    

    Use

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge