159,557 results

1. flag-hKLF6 (1006)

   Plasmid

   #49488

   Purpose

   expresses human flag tagged KLF6 in mammalian cells

   Depositor

   Scott Friedman

   Article

   Lang et al Oncogene. 2012 Oct 22. doi: 10.1038/onc.

   Insert

   KFL6 (KLF6 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

2. pDZ416 (24PP7SL loxP-Kan-loxP)

   Plasmid

   #45163

   Depositor

   Robert Singer, Daniel Zenklusen

   Article

   Hocine et al Nat Methods. 2013 Feb;10(2):119-21. doi:

   Insert

   24xPP7 Stem Loop Cassette
   

   Use

   Cre/Lox

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 29, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLV-SARS-CoV-2 5'UTR-Luciferase

   Plasmid

   #191480

   Purpose

   Luciferase reporter for SARS-CoV-2 5' UTR

   Depositor

   Hao Wu

   Article

   Vora et al Proc Natl Acad Sci U S A. 2022 Mar 1;119

   Insert

   SARS-CoV-2 5' UTR
   

   Use

   Lentiviral and Luciferase

   Tags

   Expression

   Mutation

   contains SARS-CoV-2 5' UTR

   Promoter

   Available since

   Nov. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. MTK3_038

   Plasmid

   #123752

   Purpose

   Encodes R-Flinc-A, a cAMP reporter, as a Type 3 part to be used in the MTK system

   Depositor

   Hana El-Samad

   Article

   Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

   Insert

   R-FlincA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1+FLAG-KrascomG12D

   Plasmid

   #206844

   Purpose

   To express mouse Kras encoded by common mouse codons (to increase expression) and with a G12D mutation with an N-terminal FLAG tag.

   Depositor

   Christopher Counter

   Article

   Le Roux et al Elife. 2022 Sep 7;11:e75715. doi: 10.755

   Insert

   Kras (Kras Mouse)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Codons altered to most optimum based on mouse cod…

   Promoter

   Available since

   Oct. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pYPQ-CBE-Cas9n-Act3.0

   Plasmid

   #178955

   Purpose

   It consists of a Cas9 nickase (D10A) fused with a cytidine deaminase (A3A/Y130F) and MS2-SunTag-activators (ScFv-sfGFP-2xTAD), enabling simultaneous C to T conversion and gene activation.

   Depositor

   Yiping Qi

   Article

   Pan et al Nat Plants. 2022 May;8(5):513-525. doi:

   Insert

   hA3A-zCas9-UGI-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   May 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. DsRed-rab7 DN

   Plasmid

   #12662

   Depositor

   Richard Pagano

   Article

   Choudhury et al J Clin Invest. 2002 Jun . 109(12):1541-5

   Insert

   RAB7 (Homo sapiens) (RAB7A Human)
   

   Use

   Tags

   DsRed and HA

   Expression

   Mammalian

   Mutation

   T22N

   Promoter

   Available since

   Oct. 16, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBsaBM3

   Plasmid

   #85102

   Purpose

   P450 BM3 variant (A74G/C773S/C810S)

   Depositor

   Teruyuki Nagamune

   Article

   Tan et al Angew Chem Int Ed Engl. 2016 Oct 26. doi

   Insert

   P450 BM3
   

   Use

   Cloning vector

   Tags

   Expression

   Mutation

   A74G/C773S/C810S

   Promoter

   Available since

   Dec. 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. CD44P pGL3

   Plasmid

   #19122

   Depositor

   Bob Weinberg

   Article

   Godar et al Cell. 2008 Jul 11. 134(1):62-73.

   Insert

   CD44 promoter (CD44 Human)
   

   Use

   Non viral cloning vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 3, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHR V5-RNF25 dRING_IRES-mCherry

    Plasmid

    #198387

    Purpose

    RNF25 (?RING) expression

    Depositor

    Jack Taunton

    Article

    Oltion et al Cell. 2023 Jan 19;186(2):346-362.e17. do

    Insert

    RNF25 (RNF25 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    RING deletion (135-201)

    Promoter

    EF1A

    Available since

    June 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRK5-HA-FNIP2

    Plasmid

    #72295

    Purpose

    overexpression

    Depositor

    David Sabatini

    Article

    Tsun et al Mol Cell. 2013 Nov 21;52(4):495-505. doi

    Insert

    FNIP2 (FNIP2 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

12. pMM659

    Plasmid

    #68888

    Purpose

    Chondroitin sulfate-inducible NanoLuc expression, pNBU2 backbone, AmpR

    Depositor

    Timothy Lu, Christopher Voigt

    Article

    Mimee et al Cell Syst. 2015 Jul 29;1(1):62-71. doi:

    Insert

    NanoLuc
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pHR_Gal4UAS_4D5-High-CAR-mCherry_PGK_4D5-Low-SynNotch_Gal4VP64_RHL133

    Plasmid

    #164822

    Purpose

    Lentiviral vector for Gal4 inducible AntiHER2 4D5-High CAR-mCherry and constitutive expression of antiHER2 4D5-Low Gal4VP64 Synthetic Notch

    Depositor

    Wendell Lim

    Article

    Hernandez-Lopez et al Science. 2021 Mar 12;371(6534):1166-1171

    Inserts

    AntiHER2 4D5-High Chimeric Antigen receptor (CD8alphaTM-41BB-CD3z)
    AntiHER2 4D5-Low Gal4VP64 synthetic Notch receptor
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mutation

    Promoter

    miniCMV and pGK

    Available since

    June 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pU6c:sgRNA#4

    Plasmid

    #64248

    Purpose

    expresses sgRNA under U6c promoter

    Depositor

    Wenbiao Chen

    Article

    Yin et al Genetics. 2015 Apr 8. pii: genetics.115.

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCPCbLCV.007

    Plasmid

    #22785

    Depositor

    Niki Robertson

    Article

    Turnage et al Plant J. 2002 Apr . 30(1):107-14.

    Insert

    A DNA (with MCS)+
    

    Use

    RNAi; Arabidopsis vigs vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 8, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCPCbLCVB.002

    Plasmid

    #22787

    Depositor

    Niki Robertson

    Article

    Turnage et al Plant J. 2002 Apr . 30(1):107-14.

    Insert

    B DNA
    

    Use

    RNAi; Arabidopsis vigs vector

    Tags

    Expression

    Mutation

    N/A

    Promoter

    Available since

    July 8, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCDH-EF1-copGFP

    Plasmid

    #73030

    Purpose

    Express copGFP

    Depositor

    Kazuhiro Oka

    Insert

    copGFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1

    Available since

    Feb. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. p35S:Citrine-TurboID-3xHA

    Plasmid

    #199243

    Purpose

    This plasmid could be used to clone gene(protein) of interest in place of Citrine to use in proximity labeling approach in plants.

    Depositor

    Savithramma Dinesh-Kumar

    Article

    Zhang et al Nat Commun. 2019 Jul 19;10(1):3252. doi:

    Insert

    Citrine-TurboID
    

    Use

    Tags

    3xHA and TurboID-3xHA

    Expression

    Plant

    Mutation

    Promoter

    CaMV 35S

    Available since

    June 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. NMD2

    Plasmid

    #216220

    Purpose

    Nonsense mediated decay GFP P2A RFP reporter with two b-globin introns

    Depositor

    Rebecca Voorhees

    Article

    Inglis et al J Cell Sci. 2023 May 15;136(10):jcs26121

    Insert

    GFP P2A RFP_b-globin intron x2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pPdX

    Plasmid

    #85084

    Purpose

    Expresses putidaredoxin in E. coli

    Depositor

    Teruyuki Nagamune

    Article

    Tan et al Angew Chem Int Ed Engl. 2016 Oct 26. doi

    Insert

    Putidaredoxin
    

    Use

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    changed Cysteine 73 to Serine and Cysteine 85 to …

    Promoter

    T7 promoter

    Available since

    Nov. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. HA-VHL-pRc/CMV

    Plasmid

    #19999

    Depositor

    William Kaelin

    Article

    Iliopoulos et al Nat Med. 1995 Aug . 1(8):822-6.

    Insert

    von Hippel-Lindau tumor suppressor (VHL) (VHL Human)
    

    Use

    Tags

    HA-tag

    Expression

    Mammalian

    Mutation

    wild type

    Promoter

    Available since

    April 6, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pQCH

    Plasmid

    #164160

    Purpose

    IncQ BHR plasmid

    Depositor

    Calvin Henard

    Article

    Tapscott et al Appl Environ Microbiol. 2019 May 16;85(1

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pAAV-hSyn-mCherry-sesRNA-smFlag-tTA2-W3SL

    Plasmid

    #192066

    Purpose

    Expression of mCherry, sesRNA in mammalian cells, with smFlag and tTA2 as efRNA.

    Depositor

    Josh Huang

    Article

    Qian et al Nature. 2022 Oct;610(7933):713-721. doi:

    Insert

    sesRNA for Mouse Ctip2
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn

    Available since

    Jan. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. mStrawberry-N1

    Plasmid

    #54644

    Purpose

    Localization: N1 Cloning Vector, Excitation: 574, Emission: 596

    Depositor

    Michael Davidson, Roger Tsien, Nathan Shaner

    Article

    Shaner et al Nat Biotechnol. 2004 Dec;22(12):1567-72.

    Type

    Empty backbone

    Use

    Tags

    mStrawberry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pLV puro Tet VSVG

    Plasmid

    #82724

    Purpose

    Lentiviral plasmid that expresses VSVG protein under a doxycycline-inducible promoter

    Depositor

    Warren Zipfel

    Insert

    VSVG
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pSico

    Plasmid

    #11578

    Purpose

    Cre-regulated lentiviral shRNA vector. Cre addition causes EGFP to be recombined out of the construct, activating shRNA expression.

    Depositor

    Tyler Jacks

    Article

    Ventura et al Proc Natl Acad Sci U S A. 2004 Jul 13. 1

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Cre/Lox, Lentiviral, and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 19, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. TFORF1108

    Plasmid

    #143254

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    CREBBP (CREBBP Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    June 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. phage-ubc-nls-ha-tdPCP-gfp

    Plasmid

    #40650

    Depositor

    Robert Singer

    Article

    Wu et al Biophys J. 2012 Jun 20;102(12):2936-44.

    Insert

    tdPCP-gfp
    

    Use

    Lentiviral

    Tags

    EGFP, HA, and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    human ubiquitin C (UBC)

    Available since

    Oct. 15, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. CAG-ADAR2-sesRNA-GFP-W3SL

    Plasmid

    #192069

    Purpose

    Expression of ADAR2, sesRNA in mammalian cells, with tTA2 as efRNA and ADAR2 overexpression.

    Depositor

    Josh Huang

    Article

    Qian et al Nature. 2022 Oct;610(7933):713-721. doi:

    Insert

    sesRNA for mouse Ctip2
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Cbh

    Available since

    Jan. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pHAGE-ERBB2

    Plasmid

    #116734

    Purpose

    Lentiviral expression of ERBB2

    Depositor

    Kenneth Scott, Gordon Mills

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    ERBB2 (ERBB2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    WT

    Promoter

    Available since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge