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pAAV-hSyn-GRAB_NE1m

Plasmid

#123308

Purpose

Expresses the genetically-encoded fluorescent norepinephrine(NE) sensor GRAB_NE1m in neurons

Depositor

Yulong Li

Article

Feng et al Neuron. 2019 May 22;102(4):745-761.e8. d

Has Service

AAV Retrograde and AAV9

Insert

GPCR activation based NE sensor GRAB_NE1m

Use

AAV

Mutation

contains a glycine-to-threonine mutation at posit…

Promoter

hSyn

Available Since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

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pMIAII-Cd4

Plasmid

#194401

Purpose

Retroviral expression of mouse CD4

Depositor

Michael Carroll

Article

Akama-Garren et al Sci Immunol. 2024 Feb 16;9(92):eadj7029.

Insert

Cd4 (Cd4 Mouse)

Use

Retroviral

Mutation

WT

Available Since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pLPT41

Plasmid

#85524

Purpose

Titration sponge for TetR

Depositor

Johan Paulsson

Article

Potvin-Trottier et al Nature. 2016 Oct 12;538(7626):514-517. d

Type

Empty backbone

Use

Synthetic Biology

Promoter

PLtet

Available Since

April 8, 2017

Availability

Academic Institutions and Nonprofits only

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pSIQ

Plasmid

#216238

Purpose

All-in One IQ transgene switch, empty vector

Depositor

Hyunju Ro

Article

Hong et al Mol Ther Methods Clin Dev. 2024 Feb 2;32

Type

Empty backbone

Use

Synthetic Biology

Expression

Mammalian

Available Since

May 28, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA-IgK-R-eLACCO2.1-COBRA

Plasmid

#223259

Purpose

Biosensor for extracellular L-lactate

Depositor

Robert Campbell, Yusuke Nasu

Article

Kamijo et al Nat Commun. 2025 Oct 29;16(1):9531. doi:

Insert

R-eLACCO2.1

Expression

Mammalian

Available Since

Sept. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pBMM42

Plasmid

#13331

Depositor

Martin Gellert

Article

Modesti et al EMBO J. 1999 Apr 1. 18(7):2008-18.

Insert

XRCC4 (XRCC4 Human)

Tags

His

Expression

Bacterial

Available Since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only
pABY-c45

Plasmid

#201771

Purpose

Expresses mNeonGreen-tagged NbALFA under TEF1 promoter in yeast

Depositor

Saravanan Palani

Article

Akhuli et al mSphere. 2022 Oct 26;7(5):e0033322. doi:

Insert

NbALFA

Tags

40aa Linker, mNeonGreen

Expression

Yeast

Promoter

TEF1

Available Since

Aug. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pTMiniTrex-mCherry-Shark1

Plasmid

#233143

Purpose

Serves as a template plasmid for amplifying Shark1 (Tet-OFF) hammerhead aptazyme-3xTy-tagging cassette

Depositor

Ronald Etheridge

Article

Wiedeman et al Proc Natl Acad Sci U S A. 2025 Feb 25;12

Inserts

mCherry
Shark1

Use

Prokaryotic expression

Tags

3x Ty tag

Mutation

Codon optimized for Trypanosoma cruzi and changed…

Available Since

March 25, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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AAV-P(Cry1)-DIO-intron336-Venus-NLS-D2

Plasmid

#110055

Purpose

Cry1 transcription fluorescence reporter

Depositor

Eric Erquan Zhang

Article

Mei et al Proc Natl Acad Sci U S A. 2018 Apr 2. pi

Insert

Cry1 promoter and Venus (Cry1 Mouse)

Use

AAV and Cre/Lox

Tags

NLS-D2

Expression

Mammalian

Promoter

Cry1

Available Since

May 30, 2018

Availability

Academic Institutions and Nonprofits only

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pRAM24

Plasmid

#234313

Purpose

pBBR1 origin cat-RNA U1376

Depositor

James Chappell

Article

Kalvapalle et al Nat Biotechnol. 2025 Mar 18. doi: 10.103

Insert

U1367 Targeting RAM, Barcode 1

Use

Synthetic Biology

Expression

Bacterial

Promoter

P. CymRC

Available Since

March 28, 2025

Availability

Academic Institutions and Nonprofits only

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pET-DUET-1-mRhubarb720-HO

Plasmid

#141201

Purpose

Expresses mRhubarb720 fluorescent protein and heme oxygenase

Depositor

Elad Firnberg

Article

Rogers et al Sci Rep. 2019 Oct 30;9(1):15653. doi: 10

Inserts

mRhubarb720
heme oxygenase

Tags

6x-His

Expression

Bacterial

Mutation

R134H, L196Q, T202D, V203I, W309R, Q310A compared…

Promoter

T7

Available Since

Aug. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pRVL-1

Plasmid

#104579

Purpose

Contains mouse kappa CL domain, for cloning of antibody VL domains using SapI restriction enzyme.

Depositor

Daniel Christ

Article

Vazquez-Lombardi et al Methods Mol Biol. 2018;1827:313-334. doi

Insert

Mouse CL kappa

Expression

Mammalian

Promoter

CMV

Available Since

June 11, 2018

Availability

Academic Institutions and Nonprofits only

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pJR101

Plasmid

#187241

Purpose

Lentiviral sgRNA vector for Perturb-seq with mU6 sgRNA promoter, CR1 constant region with CS1 capture sequence in stem loop, and UCOE EF1alpha driving PURO-GFP marker expression

Depositor

Marco Jost, Jonathan Weissman

Article

Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

Insert

Lentiviral sgRNA vector for Perturb-seq with mU6 promoter

Use

Lentiviral

Available Since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pMBIS

Plasmid

#17817

Depositor

Jay Keasling

Article

Martin et al Nat Biotechnol. 2003 Jul . 21(7):796-802

Insert

mevalonate kinase, phosphomevalonate kinase, mevalonate pyrophosphate decarboxylatse, IPP isomerase, FPP synthase

Use

Synthetic Biology

Expression

Bacterial

Available Since

July 27, 2008

Availability

Academic Institutions and Nonprofits only

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AP1muA-Halo-ALFA-polyA-G418 HR

Plasmid

#229681

Purpose

Homology repair plasmid for endogenous tagging of AP1muA at the C-terminus with HaloTag and an ALFA tag. Contains a G418 resistance cassette for selection of edited cells.

Depositor

Francesca Bottanelli

Article

Stockhammer et al Nat Cell Biol. 2024 Oct 4. doi: 10.1038/

Insert

genomic sequence AP1M1 locus (AP1M1 Human)

Use

CRISPR

Tags

Halo, ALFA

Available Since

Jan. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pY010 (pcDNA3.1-hAsCpf1)

Plasmid

#69982

Purpose

Expresses humanized AsCpf1

Depositor

Feng Zhang

Article

Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

Insert

hAsCpf1

Use

CRISPR

Tags

NLS-3xHA

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 8, 2015

Availability

Academic Institutions and Nonprofits only

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VPS13D^EGFP

Plasmid

#174109

Purpose

The insert is a codon optimized DNA sequence encoding VPS13D with an internal EGFP.

Depositor

Pietro De Camilli

Article

Guillen-Samander et al J Cell Biol. 2021 May 3;220(5). pii: 212

Insert

Codon optimized VPS13D

Tags

EGFP

Expression

Mammalian

Mutation

With an internal EGFP tag in aminoacid position 1…

Promoter

CMV

Available Since

Oct. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pDV06_feedback_entry_TagBFP-2A-MCS-2A-mNeonGreen-2A-MCP-ADAR2dd(E488Q)-NES

Plasmid

#200402

Purpose

Entry vector for closed-loop ADAR-based RNA sensors (DART VADAR). Expresses TagBFP constitutively. Expresses mNeonGreen and MCP-ADAR upon target detection

Depositor

James Collins

Article

Gayet et al Nat Commun. 2023 Mar 11;14(1):1339. doi:

Insert

Closed-loop DART VADAR sensor entry cassette

Use

Synthetic Biology

Expression

Mammalian

Available Since

June 30, 2023

Availability

Academic Institutions and Nonprofits only

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Cbh_v5 AAV-CBE C-terminal

Plasmid

#137176

Purpose

AAV genome: expresses the C-terminal of v5 AAV-CBE from the Cbh promoter, and U6-sgRNA

Depositor

David Liu

Article

Levy et al Nat Biomed Eng. 2020 Jan;4(1):97-110. do

Insert

v5 AAV-CBE C-terminal; U6-protospacer

Use

AAV

Promoter

Cbh

Available Since

Jan. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pSpCas9 (PX165)

Plasmid

#48137

Purpose

Human codon optimized Cas9 nuclease from Streptococcus pyogenes (Cbh-3X-FLAG-NLS-SpCas9-NLS)

Depositor

Feng Zhang

Article

Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

Insert

hSpCas9

Use

CRISPR

Tags

3XFLAG

Expression

Mammalian

Promoter

Cbh

Available Since

Oct. 29, 2013

Availability

Academic Institutions and Nonprofits only

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pMK292 (mAID-mCherry2-NeoR)

Plasmid

#72830

Purpose

C-terminal tagging with mAID-mCherry2 using CRISPR/Cas9

Depositor

Masato Kanemaki

Article

Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

Insert

mAID-mCherry2-Neo

Use

CRISPR

Tags

mAID-mCherry2

Expression

Mammalian

Available Since

Feb. 23, 2016

Availability

Academic Institutions and Nonprofits only

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Syn-ATP

Plasmid

#51819

Purpose

Optical reporter of presynaptic ATP

Depositor

Timothy Ryan

Article

Rangaraju et al Cell. 2014 Feb 13;156(4):825-35. doi: 10

Insert

Chimeric Synaptophysin-mCherry-Luciferase

Expression

Mammalian

Mutation

Within WT luciferase gene, changed Threonine 214 …

Promoter

CMV

Available Since

March 13, 2014

Availability

Academic Institutions and Nonprofits only

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pAM236 Ubc4

Plasmid

#226361

Purpose

Expression of yeast Ubc4

Depositor

Andreas Martin

Article

Bashore et al Nat Struct Mol Biol. 2015 Sep;22(9):712-

Insert

Ubc4 (UBC4 Budding Yeast)

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

Nov. 13, 2024

Availability

Academic Institutions and Nonprofits only

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TDP-REGv2(mScarlet-FLAG) matched positive control in pTwist-CMV

Plasmid

#216156

Purpose

Expresses mScarlet regardless of TDP-43 knockdown. Note: It is not recommended to produce lentiviruses containing TDP-REG sequences – see Depositor Comments

Depositor

Pietro Fratta

Article

Wilkins et al Science. 2024 Oct 4;386(6717):61-69. doi

Insert

mScarlet with C-terminal FLAG tag

Expression

Mammalian

Available Since

July 1, 2024

Availability

Academic Institutions and Nonprofits only

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35S-VP16-GWY/pAM

Plasmid

#201234

Purpose

Used to produce and express in planta C-terminal fusion proteins with the VP16 activation domain

Depositor

Michael Ayliffe

Article

Chen et al Plant J. 2023 Jun;114(6):1209-1226. doi:

Insert

35S-VP16-GWY

Expression

Plant

Promoter

CaMV 35S

Available Since

Aug. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pLVX-EF1a-EGFP-Cybt5-IRES-Puromycin

Plasmid

#134869

Purpose

Expresses EGFP-tagged endoplasmic reticulum marker

Depositor

David Andrews

Article

Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Insert

cytochrome b5 type A (CYB5A Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

deleted amino acids 1-98

Promoter

EF1a

Available Since

March 3, 2020

Availability

Academic Institutions and Nonprofits only

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pWZL Neo Myr Flag MAP2K5

Plasmid

#20514

Depositor

William Hahn, Jean Zhao

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

MAP2K5 (MAP2K5 Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Available Since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only

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pAAV2-TBG-FLAG-mRIPK1

Plasmid

#115341

Purpose

Liver-specific adeno-associated viral delivery and mammalian expression of Flag-mRIPK1

Depositor

Junying Yuan

Article

Geng et al Nat Commun. 2017 Aug 25;8(1):359. doi: 1

Insert

RIPK1 (Ripk1 Mouse)

Use

AAV

Tags

Flag

Expression

Mammalian

Available Since

Sept. 11, 2018

Availability

Academic Institutions and Nonprofits only

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ePPE-spG

Plasmid

#183096

Purpose

For plant prime editing in rice plants or monocotyledons protoplasts

Depositor

Caixia Gao

Article

Zong et al Nat Biotechnol. 2022 Mar 24. doi: 10.103

Insert

Nls-nspGCas9-NC-nls-MLV-Nls

Use

CRISPR

Expression

Plant

Mutation

D10A in SpGCas9

Available Since

April 6, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG-FADD

Plasmid

#78802

Purpose

To overexpress FADD in Mammalian Cells

Depositor

Jaewhan Song

Article

Lee et al Nat Commun. 2012;3:978. doi: 10.1038/nco

Insert

FADD (FADD Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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