8,717 results

1. M50 Super 8x TOPFlash

   Plasmid

   #12456

   Purpose

   Beta-catenin reporter. TCF/LEF sites upstream of a luciferase reporter.

   Depositor

   Randall Moon

   Article

   Veeman et al Curr Biol. 2003 Apr 15. 13(8):680-5.

   Insert

   TCF/LEF binding sites (Ctnnb1 )
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 13, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCDH-EF1-Luc2-P2A-tdTomato

   Plasmid

   #72486

   Purpose

   Express Luc2 and tdTomato under EF1 promoter

   Depositor

   Kazuhiro Oka

   Insert

   Luc2-P2A-tdTomato
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1

   Available since

   Jan. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pComb3XSS

   Plasmid

   #63890

   Purpose

   3rd generation plasmid for phage display on modified geneIII, contains stuffer fragment

   Depositor

   Carlos Barbas

   Article

   Andris-Widhopf et al J Immunol Methods. 2000 Aug 28;242(1-2):

   Inserts

   SS Stuffer
   Light Chain Stuffer
   Heavy Chain Stuffer
   

   Use

   Phage display

   Tags

   6x His, HA tag, and geneIII

   Expression

   Bacterial

   Mutation

   Promoter

   lacZ

   Available since

   July 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET28-MBP-TEV

   Plasmid

   #69929

   Purpose

   bacterial expression of MBP-TEV n-terminal fusions

   Depositor

   Thomas Wassmer, Zita Balklava

   Article

   Currinn et al Cell Mol Life Sci. 2015 Jul 28.

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Tags

   MBP and TEV cleavage site

   Expression

   Bacterial

   Mutation

   Promoter

   T7-lacO (lactose/IPTG inducible)

   Available since

   Jan. 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHR_Gal4UAS_tBFP_PGK_mCherry

   Plasmid

   #79130

   Purpose

   Lentiviral vector - tBFP reporter for Gal4DBDVP64 synNotch receptors with a constitutive mCherry

   Depositor

   Wendell Lim

   Article

   Morsut et al Cell. 2016 Feb 11;164(4):780-91. doi: 10

   Insert

   tBFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBOB-EF1-FastFUCCI-Puro

   Plasmid

   #86849

   Purpose

   3rd generation lentiviral vector encoding FUCCI reporters for cell cycle analysis and puro for selection

   Depositor

   Duncan Jodrell, Kevin Brindle

   Article

   Koh et al J Cell Sci. 2016 Nov 25. pii: jcs.195164

   Inserts

   mKO2-hCDT1(30-120)
   mAG-hGEM(1-110)
   pac (puromycin N-acetyl-transferase)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1 and PGK

   Available since

   Nov. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDAC565

   Plasmid

   #195242

   Purpose

   Mammalian expression of Csm-GFP complex (RNase mut) from separate promoters (Used for RNA imaging)

   Depositor

   Jennifer Doudna

   Article

   Colognori et al Nat Biotechnol. 2023 Jan 23. doi: 10.103

   Insert

   FLAG-NLS-Csm1; FLAG-NLS-Csm2, FLAG-NLS-Csm3(RNase mut)-GFP; FLAG-NLS-Csm4; FLAG-NLS-Csm5; FLAG-NLS-Cas6; crRNA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3-TLR9-YFP

   Plasmid

   #13642

   Depositor

   Doug Golenbock

   Insert

   Toll-Like Receptor 9 (TLR9 Human)
   

   Use

   Tags

   YFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 12, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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9. pX602-AAV-TBG::NLS-SaCas9-NLS-HA-OLLAS-bGHpA;U6::BsaI-sgRNA

   Plasmid

   #61593

   Purpose

   A single vector AAV-Cas9 system containing Cas9 from Staphylococcus aureus (SaCas9) and its sgRNA.

   Depositor

   Feng Zhang

   Article

   Ran et al Nature. 2015 Apr 1. doi: 10.1038/nature1

   Inserts

   hSaCas9
   Chimeric guide for SaCas9
   

   Use

   AAV and CRISPR

   Tags

   HA, NLS, and OLLAS tag

   Expression

   Mammalian

   Mutation

   K175R and K736R (deUb mutations)

   Promoter

   Available since

   Feb. 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAG148 FRB-NFAST

    Plasmid

    #130812

    Purpose

    Expresses FRB-NFAST in mammalian cells

    Depositor

    Arnaud Gautier

    Article

    Tebo et al Nat Commun. 2019 Jun 27;10(1):2822. doi:

    Insert

    FRB-NFAST
    

    Use

    Tags

    myc-tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEGFP Vinculin

    Plasmid

    #50513

    Depositor

    Kenneth Yamada

    Insert

    Vinculin (VCL Chicken)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. lentiCRISPRv2 blast

    Plasmid

    #98293

    Purpose

    Variant of lentiCRISPRv2 that confers blasticidin S resistance

    Depositor

    Brett Stringer

    Article

    Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF-1a

    Available since

    July 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. lenti sgRNA(MS2)_puro optimized backbone

    Plasmid

    #73797

    Purpose

    optimized lenti sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2 and EF1a-puro resistance marker. Contains BsmBI sites for insertion of spacer sequences.

    Depositor

    Feng Zhang

    Article

    Joung et al Nat Protoc. 2017 Apr;12(4):828-863. doi:

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and EF1A

    Available since

    March 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3 MDM2 WT

    Plasmid

    #16233

    Depositor

    Mien-Chie Hung

    Article

    Zhou et al Nat Cell Biol. 2001 Nov . 3(11):973-82.

    Insert

    MDM2 (MDM2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 30, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pMF230

    Plasmid

    #62546

    Purpose

    Broad host range plasmid for constitutive expression of the eGFP. Developed for imaging Pseudomonas aeruginosa in biofilms.

    Depositor

    Michael Franklin

    Article

    Nivens et al J Bacteriol. 2001 Feb;183(3):1047-57.

    Insert

    GFP mut2
    

    Use

    Broad host range plasmid

    Tags

    Expression

    Mutation

    Promoter

    trc

    Available since

    April 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. MP6

    Plasmid

    #69669

    Purpose

    Untargetted Bacterial Mutagenesis

    Depositor

    David Liu

    Article

    Badran et al Nat Commun. 2015 Oct 7;6:8425. doi: 10.1

    Insert

    dnaQ926 dam seqA emrR ugi CDA1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    E. coli PBAD

    Available since

    Oct. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSANG10-3F-BG4

    Plasmid

    #55756

    Purpose

    Expresses BG4 scFv anti-G-quadruplex antibody in bacteria

    Depositor

    Shankar Balasubramanian

    Article

    Biffi et al Nat Chem. 2013 Mar;5(3):182-6. doi: 10.1

    Insert

    BG4
    

    Use

    Tags

    6xHis, pelB leader, and tri-FLAG

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCMV-Flag-YAP-5SA/S94A

    Plasmid

    #33103

    Depositor

    Kunliang Guan

    Article

    Zhao et al Genes Dev. 2008 Jul 15. 22(14):1962-71.

    Insert

    YAP (YAP1 )
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    S61A, S94A, S109A, S127A, S164A, S381A

    Promoter

    Available since

    Feb. 10, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET3a-hUBA1

    Plasmid

    #63571

    Purpose

    Expression of human UBA1 tagged with His

    Depositor

    Titia Sixma

    Article

    Pichler et al Nat Struct Mol Biol. 2005 Mar;12(3):264-

    Insert

    UBA1 (UBA1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Isoform 2 (1018 amino acids)

    Promoter

    T7

    Available since

    March 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. H2B-GFP

    Plasmid

    #11680

    Depositor

    Geoff Wahl

    Article

    Kanda et al Curr Biol. 1998 Mar 26. 8(7):377-85.

    Insert

    Human histone H2B (H2BC11 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 31, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge