8,806 results

1. pLV hUbC-dCas9 KRAB-T2A-GFP

   Plasmid

   #67620

   Purpose

   Co-expressed human optimized S. pyogenese dCas9 fused to KRAB repressor domain and GFP

   Depositor

   Charles Gersbach

   Article

   Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

   Insert

   humanized dead Cas9 KRAB T2A GFP
   

   Use

   CRISPR and Lentiviral

   Tags

   Flag

   Mutation

   D10A and H840A

   Available Since

   March 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCXN2-BCL6 (CXN-BCL6)

   Plasmid

   #40346

   Depositor

   Alexander Dent

   Article

   Vasanwala et al J Immunol. 2002 Aug 15;169(4):1922-9.

   Insert

   BCL6 (BCL6 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   pCAG

   Available Since

   Oct. 15, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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3. HA-RNF4/pRK5

   Plasmid

   #59743

   Purpose

   Expresses RNF4 in mammalian cells

   Depositor

   Xiaolu Yang

   Article

   Guo et al Mol Cell. 2014 Jul 3;55(1):15-30. doi: 1

   Insert

   RNF4 (RNF4 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 27, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAB46 (CK2beta)

   Plasmid

   #27085

   Depositor

   David Litchfield

   Article

   Turowec et al Methods Enzymol. 2010;484:471-93.

   Insert

   CK2beta (CSNK2B Human)
   

   Tags

   His

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   May 17, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-CaMKIIa-HA-KORD-IRES-mCitrine

   Plasmid

   #65418

   Purpose

   AAV packaging vector for CamKIIa driven KOR DREADD expression

   Depositor

   Bryan Roth

   Article

   Vardy et al Neuron. 2015 Apr 29. pii: S0896-6273(15)

   Insert

   CaMKIIa-HA-KORD-IRES-mCitrine (OPRK1 Human)
   

   Use

   AAV

   Tags

   HA and mCitrine

   Promoter

   CaMKIIa

   Available Since

   June 5, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. SypHer

   Plasmid

   #48250

   Purpose

   pH sensor

   Depositor

   Nicolas Demaurex

   Article

   Poburko et al J Biol Chem. 2011 Apr 1;286(13):11672-84

   Insert

   SypHer
   

   Expression

   Mammalian

   Mutation

   C199S

   Promoter

   CMV

   Available Since

   Oct. 28, 2013

   Availability

   Academic Institutions and Nonprofits only

7. pLentiRNACRISPR_006 - hU6-DR_BsmBI-EFS-RfxCas13d-NES-2A-Puro-WPRE

   Plasmid

   #138148

   Purpose

   Expresses RfxCas13d in mammalian cells, localized in the cytoplasm. For cloning of guide RNAs compatible with RfxCas13d. Contains a 5' direct repeat. Clone using BsmBI. F overhang aaac. R overhang aaaa

   Depositor

   Neville Sanjana

   Article

   Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.

   Insert

   CasRx
   

   Use

   Lentiviral

   Tags

   NES

   Available Since

   Dec. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSin-EF2-Nanog-Pur

   Plasmid

   #16578

   Depositor

   James Thomson

   Article

   Yu et al Science. 2007 Dec 21. 318(5858):1917-20.

   Insert

   Homo sapiens Nanog homeobox (NANOG Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Jan. 4, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAD48-daf-2 RNAi

   Plasmid

   #34834

   Depositor

   Cynthia Kenyon

   Article

   Dillin et al Science. 2002 Oct 25;298(5594):830-4.

   Insert

   daf-2 (daf-2 Nematode)
   

   Use

   RNAi

   Expression

   Bacterial

   Available Since

   June 11, 2012

   Availability

   Academic Institutions and Nonprofits only

10. mADAM17 in pcDNA3.1

    Plasmid

    #19141

    Depositor

    Zena Werb

    Article

    Lemieux et al J Biol Chem. 2007 May 18. 282(20):14836-

    Insert

    ADAM17 (Adam17 Mouse)
    

    Expression

    Mammalian

    Available Since

    Sept. 8, 2008

    Availability

    Academic Institutions and Nonprofits only

11. pMSP2N2

    Plasmid

    #29520

    Depositor

    Stephen Sligar

    Article

    Grinkova et al Protein Eng Des Sel. 2010 Nov . 23(11):8

    Insert

    MSP2N2
    

    Tags

    7-His tag

    Expression

    Bacterial

    Mutation

    tandem MSP; His-tag on N-terminus followed by spa…

    Available Since

    May 3, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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12. pimEJ5GFP

    Plasmid

    #44026

    Depositor

    Jeremy Stark

    Article

    Bennardo et al PLoS Genet. 2008 Jun 27;4(6):e1000110. d

    Insert

    EJ5GFP egfp-based chromosomal break reporter
    

    Expression

    Mammalian

    Mutation

    pCAGGS promoter and eGFP separated by pgkPURO cas…

    Promoter

    pCAGGS

    Available Since

    May 3, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRS316-RGR-GFP

    Plasmid

    #51056

    Purpose

    Express self-cleaving-ribozyme-flanked sgRNA cassette (RGR) targeting GFP for CRISPR systems in yeast driven by ADH1 promoter. RGR has a HH ribozyme at its 5', and an HDV ribozyme at its 3'.

    Depositor

    Yunde Zhao

    Article

    Gao et al J Integr Plant Biol. 2013 Dec 30. doi: 1

    Insert

    RGR-GFP
    

    Use

    CRISPR

    Expression

    Bacterial and Yeast

    Promoter

    Yeast ADH1 promoter

    Available Since

    Feb. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMitoTimer

    Plasmid

    #52659

    Purpose

    Expresses mitochondrial targeted DsRed1-E5 as reporter for mitochondrial content, structure, oxidative stress and damage in vitro and in vivo

    Depositor

    Zhen Yan

    Article

    Laker et al J Biol Chem. 2014 Apr 25;289(17):12005-1

    Insert

    DsRed1-E5
    

    Use

    Dsred

    Tags

    human cytochrome c oxidase subunit VIII mitochond…

    Promoter

    CMV

    Available Since

    April 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCE-mp53DD

    Plasmid

    #41856

    Purpose

    Non-integrating (episomal) expression of mouse p53DD - p53 carboxy-terminal dominant-negative fragment

    Depositor

    Shinya Yamanaka

    Article

    Okita et al Stem Cells. 2012 Nov 29. doi: 10.1002/st

    Insert

    Trp53 (Trp53 Mouse)
    

    Expression

    Mammalian

    Mutation

    Δ40-903

    Promoter

    CAG

    Available Since

    July 18, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTight-9-124-BclxL

    Plasmid

    #60857

    Purpose

    2nd generation lentiviral transfer plasmid. Expresses a synthetic cluster of miR-9/9* and miR-124 fused to Bcl-xL under a doxycycline-inducible promoter

    Depositor

    Andrew Yoo

    Article

    Victor et al Neuron. 2014 Oct 22;84(2):311-23. doi: 1

    Insert

    miR-9/9*-miR-124-BCLxL (BCL2L1 Mouse)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    pTight

    Available Since

    Dec. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET-28a_6H-TAQ_E602D

    Plasmid

    #166944

    Purpose

    Bacterial expression of Taq DNA polymerase

    Depositor

    Robert Tjian

    Article

    Graham et al PLoS One. 2021 Feb 3;16(2):e0246647. doi

    Insert

    Thermus aquaticus DNA polymerase
    

    Tags

    6X His

    Expression

    Bacterial

    Mutation

    E602D

    Promoter

    T7

    Available Since

    April 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET_mNG2(1-10)_32aalinker_mNG2(11)

    Plasmid

    #82611

    Purpose

    Bacterial expression vector for split mNeonGreen2 screening

    Depositor

    Bo Huang

    Article

    Feng et al Nat Commun. 2017 Aug 29;8(1):370. doi: 1

    Insert

    mNeonGreen2(1-10)_32aalinker_mNeonGreen2(11)
    

    Expression

    Bacterial

    Mutation

    split before mutations

    Promoter

    T7

    Available Since

    May 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. NG-ABEmax

    Plasmid

    #124163

    Purpose

    A-to-G base editor

    Depositor

    David Liu

    Article

    Huang et al Nat Biotechnol. 2019 May 20. pii: 10.103

    Insert

    TadA-TadA(evo)-Cas9-NG
    

    Expression

    Mammalian

    Available Since

    May 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCDNA3.1-FLAG-SF3B1-WT

    Plasmid

    #82576

    Purpose

    Expresses a codon-optimized ORF of human SF3B1 (wild-type)

    Depositor

    Manoj Pillai

    Article

    Kesarwani et al Oncogene. 2016 Aug 15. doi: 10.1038/onc.

    Insert

    human SF3B1-WT (SF3B1 Human)
    

    Tags

    FLAG peptide

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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