We narrowed to 24,925 results for: crispr

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pRS712

Plasmid

#213041

Purpose

CRISPR activation based overexpression in Nakaseomyces (Candida) glabrata

Depositor

Rebecca Shapiro

Publication

Maroc et al mSphere. 2024 Feb 28;9(2):e0076123. doi:

Type

Empty backbone

Expression

Yeast

Available since

Jan. 24, 2024

Availability

Academic Institutions and Nonprofits only

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Lenti-EF1alpha-VP64-dCas9-p300_Blast

Plasmid

#192654

Purpose

3rd generation lenti vector encoding VP64-dCas9-p300 with 2A Blast resistance marker

Depositor

Jeffrey Miner

Publication

Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

Insert

VP64-dCas9-p300

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

D10A; H840A in Cas9

Promoter

EF1a

Available since

March 22, 2023

Availability

Academic Institutions and Nonprofits only

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pHAGE-IRES-puro-NLS-NLS-dPba3Cas13b-3xGFP-NLS

Plasmid

#191369

Purpose

Overexpression of NLS-NLS-dPba3Cas13b-3xGFP-NLS in human cells

Depositor

Ling-Ling Chen

Publication

Yang et al Cell Insight. 2022 Jun 15;1(4):100044. d

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

NLS-NLS-dPba3Cas13b-3xGFP-NLS

Tags

3xGFP

Expression

Mammalian

Available since

Oct. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pCJH027_SC101_pLAC_LbCas12a_Kan

Plasmid

#183074

Purpose

Wild-type LbCas12a (Chen et al., 2018) in a plasmid containing a SC101 origin and lac promoter for in vivo interference assay.

Depositor

Jennifer Doudna

Publication

Huang et al Mol Cell. 2022 Jun 2;82(11):2148-2160.e4

Insert

LbCas12a

Expression

Bacterial

Mutation

Wild-type

Available since

June 2, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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BC1441-mouse U6-3xsgRNAs (TS5, TS6, TS7)

Plasmid

#164040

Purpose

Expression of three sgRNAs (sgTS5, sgTS6, sgTS7) for targeting to CRISPR-Tag_V2

Depositor

Baohui Chen

Publication

Chen et al Nat Commun. 2018 Nov 29;9(1):5065. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

sgTS5-sgTS6-sgTS7

Use

CRISPR

Promoter

mouse U6

Available since

Jan. 28, 2021

Availability

Academic Institutions and Nonprofits only

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sgRNA AAVS1

Plasmid

#128119

Purpose

Co-expresses sgRNA AAVS1, SpyCas9 scaffold (F+E) and GFP (transfection marker)

Depositor

Dominik Niopek

Publication

Aschenbrenner et al Sci Adv. 2020 Feb 5;6(6):eaay0187. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

sgRNA AAVS1 + GFP

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Promoter

RSV/U6

Available since

Feb. 13, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pYZ293

Plasmid

#102687

Purpose

S. pombe gRNA entry vector with Cas9 - Sp leu1 marker

Depositor

Jef Boeke

Publication

Zhao et al G3 (Bethesda). 2018 Jan 10. pii: g3.117.

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available since

April 5, 2019

Availability

Academic Institutions and Nonprofits only

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pYZ294

Plasmid

#102688

Purpose

S. pombe gRNA entry vector with Cas9 - KanMX marker

Depositor

Jef Boeke

Publication

Zhao et al G3 (Bethesda). 2018 Jan 10. pii: g3.117.

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available since

April 5, 2019

Availability

Academic Institutions and Nonprofits only

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pYZ300

Plasmid

#102689

Purpose

S. pombe gRNA entry vector with Cas9 - Sp his3 marker

Depositor

Jef Boeke

Publication

Zhao et al G3 (Bethesda). 2018 Jan 10. pii: g3.117.

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available since

April 5, 2019

Availability

Academic Institutions and Nonprofits only

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pYZ301

Plasmid

#102690

Purpose

S. pombe gRNA entry vector with Cas9 - Sp lys9 marker

Depositor

Jef Boeke

Publication

Zhao et al G3 (Bethesda). 2018 Jan 10. pii: g3.117.

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available since

April 5, 2019

Availability

Academic Institutions and Nonprofits only

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pCAG-AaCas12b(R911A)-2AeGFP

Plasmid

#121961

Purpose

Mammalian expression, Transcription regulation, deactivated AaCas12b

Depositor

Wei Li

Publication

Teng et al Cell Discov. 2018 Nov 27;4:63. doi: 10.1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Catalytically deactivated AaCas12b

Tags

3xHA tag

Expression

Mammalian

Mutation

R911A

Available since

Feb. 14, 2019

Availability

Academic Institutions and Nonprofits only

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gh40

Plasmid

#106714

Purpose

expression of gRNA targeting ST6GAL1

Depositor

Eric-Paul Bennett

Publication

Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

ST6GAL1 (ST6GAL1 Human)

Expression

Mammalian

Available since

Jan. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pEPOR0SP0020

Plasmid

#117531

Purpose

Level0 Golden Gate part: CDS, SaCas9

Depositor

Nicola Patron

Publication

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

SaCas9 (with stop codon)

Use

CRISPR

Available since

Nov. 13, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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gh76

Plasmid

#106747

Purpose

expression of gRNA targeting MGAT1

Depositor

Eric-Paul Bennett

Publication

Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

MGAT1 (MGAT1 Human)

Expression

Mammalian

Available since

Nov. 9, 2018

Availability

Academic Institutions and Nonprofits only

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gh19

Plasmid

#106703

Purpose

expression of gRNA targeting GALNT19

Depositor

Eric-Paul Bennett

Publication

Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

GALNT19

Expression

Mammalian

Available since

Nov. 9, 2018

Availability

Academic Institutions and Nonprofits only

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sg2.02 MUC4.1

Plasmid

#101152

Purpose

gRNA with two MCP binding sites

Depositor

Mazhar Adli

Publication

Qin et al Nat Commun. 2017 Mar 14;8:14725. doi: 10

Insert

MUC4 (MUC4 Human)

Use

CRISPR and Lentiviral

Promoter

mU6

Available since

Nov. 9, 2017

Availability

Academic Institutions and Nonprofits only

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pJB166

Plasmid

#86998

Purpose

CRISPR/Cas9 in fission yeast using fluoride selection

Depositor

Julien Berro

Publication

Fernandez et al Yeast. 2016 Oct;33(10):549-557. doi: 10.

Type

Empty backbone

Expression

Bacterial and Yeast

Available since

Feb. 22, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pX459_HR_Prnp_3

Plasmid

#78623

Purpose

pX459 vector encoding SpCas9-2A-Puro and a chimeric guide RNA targeting Prnp coding sequence

Depositor

Walker Jackson

Publication

Kaczmarczyk et al PLoS One. 2016 Apr 29;11(4):e0154604. do

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

HR_Prnp_3 (Prnp Mouse)

Use

CRISPR and Mouse Targeting

Expression

Mammalian

Promoter

U6

Available since

July 19, 2016

Availability

Academic Institutions and Nonprofits only

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sgRNA2_IL1RN

Plasmid

#64142

Purpose

Photoactivatable transcription system. Lentiviral expression of IL1RN sgRNA2. Also contains a CMV-puro-t2A-mCherry expression cassette

Depositor

Moritoshi Sato

Publication

Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

Insert

sgRNA2_IL1RN (IL1RN Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available since

April 15, 2015

Availability

Academic Institutions and Nonprofits only

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sgRNA4_IL1RN

Plasmid

#64152

Purpose

Photoactivatable transcription system. Lentiviral expression of IL1RN sgRNA4. Also contains a CMV-puro-t2A-mCherry expression cassette

Depositor

Moritoshi Sato

Publication

Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

Insert

sgRNA4_IL1RN (IL1RN Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available since

April 15, 2015

Availability

Academic Institutions and Nonprofits only

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