We narrowed to 16,819 results for: IGH@

1. pCRISPomyces-1

   Plasmid

   #61736

   Purpose

   Streptomyces expression of codon-optimized Cas9, tracrRNA, and custom crRNA

   Depositor

   Huimin Zhao

   Article

   Cobb et al ACS Synth Biol. 2014 Dec 8.

   Inserts

   sSpCas9
   tracrRNA
   crRNA cassette
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   BbsI-flanked lacZ cassette inserted in place of s…

   Promoter

   gapdhp(EL), rpsLp(CF), and rpsLp(XC)-BbsI

   Available since

   Jan. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pHR-CAG_3C-Twin-Strep

   Plasmid

   #113901

   Purpose

   Mammalian protein expression

   Depositor

   A. Radu Aricescu

   Article

   Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   3C-Twin-Strep

   Expression

   Mutation

   Promoter

   CAG

   Available since

   Aug. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pQE-80L MBP-SspB Micro

   Plasmid

   #60410

   Purpose

   E. coli expression of SspB Micro

   Depositor

   Brian Kuhlman

   Article

   Guntas et al Proc Natl Acad Sci U S A. 2015 Jan 6;112

   Insert

   SspB micro
   

   Use

   Tags

   His-MBP-TEV

   Expression

   Bacterial

   Mutation

   R73Q, Y11K and A15E

   Promoter

   Available since

   Feb. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pGWB552

   Plasmid

   #74882

   Purpose

   Gateway cloning compatible binary vector for N-terminal fusion with G3GFP (CaMV35S promoter).

   Depositor

   Tsuyoshi Nakagawa

   Article

   Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

   Type

   Empty backbone

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   CaMV35S

   Available since

   Oct. 18, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. dCas9-CRY2Clust-BRD4ΔN

   Plasmid

   #200968

   Purpose

   induction of phase-separated condensate mediated by BRD4dN-IDR

   Depositor

   Jian Xu

   Article

   Kim et al Sci Adv. 2023 Mar 31;9(13):eadg1123. doi

   Insert

   BRD4dN
   

   Use

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available since

   Aug. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pROF417

   Plasmid

   #155335

   Purpose

   Constitutive expression LFY-VP16-2A-RLuc in plants

   Depositor

   Matias Zurbriggen

   Article

   Ochoa-Fernandez et al Nat Methods. 2020 Jul;17(7):717-725. doi

   Insert

   PCaMV35S-LFY-VP16-NLS-2A-RLuc-Tnos
   

   Use

   Luciferase and Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Oct. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. TRE3G-CTCF-WT-HA-MND-zeo

   Plasmid

   #232801

   Purpose

   Inducible over expression of wild type version of CTCF.

   Depositor

   Chunliang Li

   Article

   Hyle et al Genome Biol. 2023 Jan 26;24(1):14. doi:

   Insert

   CTCF (Ctcf Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   WT

   Promoter

   Available since

   Feb. 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. BA31_pU.CAG.SaCas9-D10A.rBGpA

   Plasmid

   #199251

   Purpose

   Expression construct encoding the S. aureus Cas9.D10A nickase (SaCas9.D10A)

   Depositor

   Manuel Goncalves

   Article

   Wang et al Nucleic Acids Res. 2021 Jan 25;49(2):117

   Insert

   SaCas9.D10A nickase
   

   Use

   CRISPR

   Tags

   3XHA epitope, Nucleoplasmin NLS, and SV40 NLS

   Expression

   Mammalian

   Mutation

   D10A

   Promoter

   CAG promoter

   Available since

   April 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. CVS-N2c-jGCaMP8s

   Plasmid

   #172389

   Purpose

   Production of RVdG-CVS-N2c viral vectors for cell-type specific trans-synaptic retrograde labeling.

   Depositor

   Yoav Ben-Simon, Peter Jonas

   Article

   Sumser et al Elife. 2022 Aug 30;11:e79848. doi: 10.75

   Insert

   GCaMP8s
   

   Use

   Neurotropic virus

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. AZ64_pE.DonorCLYBL.TS

    Plasmid

    #199228

    Purpose

    Donor plasmid with CLYBL target sites for ITPN or HMEJ knock-in at the human CLYBL safe harbor locus

    Depositor

    Manuel Goncalves

    Article

    Wang et al Nucleic Acids Res. 2023 Mar 17:gkad165.

    Insert

    Expression unit for EGFP reporter
    

    Use

    Gene targeting donor plasmid

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Human PGK1 gene promoter

    Available since

    April 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pENTR-PKAKTRClover

    Plasmid

    #59146

    Purpose

    ORF encoding PKA KTR mClover flanked by gateway sequences

    Depositor

    Markus Covert

    Article

    Regot et al Cell. 2014 Jun 19;157(7):1724-34. doi: 1

    Insert

    PKA Kinase Translocation Reporter (PRKACA Human, Mouse)
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. Rap1A FLARE

    Plasmid

    #134926

    Purpose

    Rap1A biosensor

    Depositor

    Klaus Hahn

    Article

    O'Shaughnessy et al J Cell Biol. 2019 Sep 2;218(9):3153-3160

    Insert

    Rap1A, mCerulean3, RaIGDS RBD, YPet
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pRSFDuet-1_VNp6-DARPinOFF7

    Plasmid

    #182398

    Purpose

    Bacterial expression of Vesicle Nucleating peptide6-DARPinOFF7 fusion

    Depositor

    Dan Mulvihill

    Article

    Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

    Insert

    VNp6-DARPinOFF7
    

    Use

    Tags

    Vesicle Nucleating peptide 6 (VNp6)

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAGM65879

    Plasmid

    #153214

    Purpose

    Level 2 cloning vector for one or several guide RNAs, already contains Cas9 and Bar selection cassette

    Depositor

    Sylvestre Marillonnet

    Article

    Grutzner et al Plant Commun. 2020 Nov 23;2(2):100135. d

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pKanCMV-mRuby3-18aa-Tubulin

    Plasmid

    #74256

    Purpose

    Mammalian expression of N-terminal fusion of mRuby3 to Tubulin

    Depositor

    Michael Lin

    Article

    Bajar et al Sci Rep. 2016 Feb 16;6:20889. doi: 10.10

    Insert

    Tubulin
    

    Use

    Tags

    mRuby3

    Expression

    Mammalian

    Mutation

    Promoter

    CAG and CMV

    Available since

    April 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. Rap1B FLARE

    Plasmid

    #134927

    Purpose

    Rap1B biosensor

    Depositor

    Klaus Hahn

    Article

    O'Shaughnessy et al J Cell Biol. 2019 Sep 2;218(9):3153-3160

    Insert

    RaIGDS RBD, YPet, Cerulean, Rap1B
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pMTH PKC alpha

    Plasmid

    #8409

    Depositor

    Frederic Mushinski

    Article

    Mischak et al J Biol Chem 1993 Sep 25;268(27):20110-5.

    Insert

    Mouse Protein Kinase C alpha (Prkca Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 5, 2007

    Availability

    Academic Institutions and Nonprofits only

18. pQE-80L iLID (C530M)

    Plasmid

    #60408

    Purpose

    E. coli expression of iLID (C530M)

    Depositor

    Brian Kuhlman

    Article

    Guntas et al Proc Natl Acad Sci U S A. 2015 Jan 6;112

    Insert

    iLID C530M
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    C530M

    Promoter

    Available since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

19. pKLV2-EF1adCas9VP64T2ABsd-W

    Plasmid

    #112919

    Purpose

    Lentiviral vector expressing Lentiviral vector expressing Lentiviral vector expressing dCas9VP64 under the control of the long human EF1a promoter

    Depositor

    Kosuke Yusa

    Article

    Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

    Insert

    dCas9VP64-T2A-Blast
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Human EF1a promoter

    Available since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pIBA3-SpyCatcher-sfGFP

    Plasmid

    #107420

    Purpose

    For production of SpyCatcher protein fused N-terminally to sfGFP. Includes N-terminal His tag for purification and C-terminal StrepII tag for detection.

    Depositor

    Jack Leo

    Article

    Chauhan et al Mol Microbiol. 2019 Jan 1. doi: 10.1111/

    Insert

    SpyCatcher
    

    Use

    Tags

    His tag, StrepII, and superfolder GFP

    Expression

    Bacterial

    Mutation

    Promoter

    Tet

    Available since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pBAD/HisD-PATagRFP

    Plasmid

    #31945

    Depositor

    Vladislav Verkhusha

    Article

    Subach et al J Am Chem Soc. 2010 May 12;132(18):6481-

    Insert

    PATagRFP
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    R69S/F84W/Q139K/A147P/N148S/M151K/Y153K/S165V/H20…

    Promoter

    araB

    Available since

    Sept. 6, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. Cas12j-8-puro

    Plasmid

    #194966

    Purpose

    The plasmid expresses human codon-optimized Cas12j-8 and puromycin resistence gene.

    Depositor

    Yongming Wang

    Article

    Wang et al Sci Adv. 2023 Feb 10;9(6):eabo6405. doi:

    Type

    Empty backbone

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pcDNA3.3_NDG

    Plasmid

    #26820

    Depositor

    Derrick Rossi

    Article

    Warren et al Cell Stem Cell. 2010 Nov 5;7(5):618-30.

    Insert

    5'UTR-NDGFP-3'UTR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    nuclear destabilized eGFP

    Promoter

    Available since

    Nov. 17, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

24. pCDNA3.1neo-NLucYAP15

    Plasmid

    #107610

    Purpose

    measure LATS activity by Luciferase Assay

    Depositor

    Xiaolong Yang

    Article

    Azad et al Nat Commun. 2018 Mar 13;9(1):1061. doi:

    Insert

    NLucYAP15
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. mEos2-Mito-7

    Plasmid

    #57401

    Purpose

    Localization: Mitochondria, Excitation: 505 / 569, Emission: 516 / 581

    Depositor

    Michael Davidson

    Article

    McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

    Insert

    Mito (COX8A Human)
    

    Use

    Tags

    mEos2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 5, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

26. pConRef-2H12

    Plasmid

    #221182

    Purpose

    cdGreen expressed from a constitutive promoter in an operon with mScarlet-I (downstream) as a reference FP (Internal Lab ID: UJ11213)

    Depositor

    Urs Jenal

    Article

    Kaczmarczyk et al Nat Commun. 2024 May 9;15(1):3920. doi:

    Inserts

    cdGreen
    mScarlet-I
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    N-terminus changed: starts with MSKKYGEAVIKE ...

    Promoter

    Constitutive and None (same as cdGreen)

    Available since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pVSVG-PATagRFP

    Plasmid

    #31947

    Depositor

    Vladislav Verkhusha

    Article

    Subach et al J Am Chem Soc. 2010 May 12;132(18):6481-

    Insert

    PATagRFP
    

    Use

    Tags

    VSVG

    Expression

    Mammalian

    Mutation

    R69S/F84W/Q139K/A147P/N148S/M151K/Y153K/S165V/H20…

    Promoter

    CMV

    Available since

    Sept. 22, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. CVS-N2c-nl.EGFP-SypGFP

    Plasmid

    #172380

    Purpose

    Production of RVdG-CVS-N2c viral vectors for cell-type specific trans-synaptic retrograde labeling.

    Depositor

    Yoav Ben-Simon, Peter Jonas

    Article

    Sumser et al Elife. 2022 Aug 30;11:e79848. doi: 10.75

    Insert

    nuclear-localized EGFP + SypEGFP
    

    Use

    Neurotropic virus

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pCAG-ChRmine-mRuby2-ST

    Plasmid

    #227777

    Purpose

    Cation channelrhodopsin ChRmine fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendrites for optogenetic excitation

    Depositor

    Hillel Adesnik

    Article

    Sridharan et al Neuron. 2022 Jan 27. pii: S0896-6273(22)

    Insert

    ChRmine-mRuby2-ST
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pGWB435

    Plasmid

    #74825

    Purpose

    Gateway cloning compatible binary vector for C-terminal fusion with LUC (no promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Sept. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge