We narrowed to 29,856 results for: REP

1. pHAGE2-TetO-SOX17E57T

   Plasmid

   #216192

   Purpose

   Generate lentiviruses encoding for human SOX17E57T (HMG box numbering); for use in pluripotency reprogramming

   Depositor

   Ralf Jauch

   Article

   Ho et al Stem Cell Reports. 2025 Mar 11;20(3):102

   Insert

   SOX17 (SOX17 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Glu113Glu (silent mutation); Glu120Glu (silent mu…

   Promoter

   Available Since

   July 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pHAGE2-TetO-SOX17E57V

   Plasmid

   #216193

   Purpose

   Generate lentiviruses encoding for human SOX17E57V (HMG box numbering); for use in pluripotency reprogramming

   Depositor

   Ralf Jauch

   Article

   Ho et al Stem Cell Reports. 2025 Mar 11;20(3):102

   Insert

   SOX17 (SOX17 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Glu113Glu (silent mutation); Glu120Glu (silent mu…

   Promoter

   Available Since

   July 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pHAGE2-TetO-SOX17E57W

   Plasmid

   #216194

   Purpose

   Generate lentiviruses encoding for human SOX17E57W(HMG box numbering); for use in pluripotency reprogramming

   Depositor

   Ralf Jauch

   Article

   Ho et al Stem Cell Reports. 2025 Mar 11;20(3):102

   Insert

   SOX17 (SOX17 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Glu113Glu (silent mutation); Glu120Glu (silent mu…

   Promoter

   Available Since

   July 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pHAGE2-TetO-SOX17E57Y

   Plasmid

   #216195

   Purpose

   Generate lentiviruses encoding for human SOX17E57Y (HMG box numbering); for use in pluripotency reprogramming

   Depositor

   Ralf Jauch

   Article

   Ho et al Stem Cell Reports. 2025 Mar 11;20(3):102

   Insert

   SOX17 (SOX17 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Glu113Glu (silent mutation); Glu120Glu (silent mu…

   Promoter

   Available Since

   July 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pHAGE2-TetO-SOX17E57K

   Plasmid

   #216184

   Purpose

   Generate lentiviruses encoding for human SOX17E57K (HMG box numbering); for use in pluripotency reprogramming

   Depositor

   Ralf Jauch

   Article

   Ho et al Stem Cell Reports. 2025 Mar 11;20(3):102

   Insert

   SOX17 (SOX17 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Glu113Glu (silent mutation); Glu120Glu (silent mu…

   Promoter

   Available Since

   July 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pHAGE2-TetO-SOX17E57L

   Plasmid

   #216185

   Purpose

   Generate lentiviruses encoding for human SOX17E57L (HMG box numbering); for use in pluripotency reprogramming

   Depositor

   Ralf Jauch

   Article

   Ho et al Stem Cell Reports. 2025 Mar 11;20(3):102

   Insert

   SOX17 (SOX17 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Glu113Glu (silent mutation); Glu120Glu (silent mu…

   Promoter

   Available Since

   July 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pHAGE2-TetO-SOX17E57M

   Plasmid

   #216186

   Purpose

   Generate lentiviruses encoding for human SOX17E57M (HMG box numbering); for use in pluripotency reprogramming

   Depositor

   Ralf Jauch

   Article

   Ho et al Stem Cell Reports. 2025 Mar 11;20(3):102

   Insert

   SOX17 (SOX17 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Glu113Glu (silent mutation); Glu120Glu (silent mu…

   Promoter

   Available Since

   July 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pHAGE2-TetO-SOX17E57N

   Plasmid

   #216187

   Purpose

   Generate lentiviruses encoding for human SOX17E57N (HMG box numbering); for use in pluripotency reprogramming

   Depositor

   Ralf Jauch

   Article

   Ho et al Stem Cell Reports. 2025 Mar 11;20(3):102

   Insert

   SOX17 (SOX17 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Glu113Glu (silent mutation); Glu120Glu (silent mu…

   Promoter

   Available Since

   July 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pLV-TET1-dCas9

   Plasmid

   #235599

   Purpose

   Expresses Tet1CD-dCas9

   Depositor

   Ting Zhou

   Article

   Wu et al Stem Cell Reports. 2025 Apr 25:102499. d

   Insert

   TET1-dCas9 (TET1 Human, SpCas9 is from Streptococcus pyogenes)
   

   Use

   CRISPR and Lentiviral

   Tags

   3xNLS and TET1 CD

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   June 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCAGEN-Luciferase-P2A-H2A-mCherry (JDW 1129)

    Plasmid

    #229823

    Purpose

    A CAGGS driven luciferase reporter followed by a P2A cleavage peptide and an H2A mCherry cassette for nuclear labeling.

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    Luciferase-P2A-H2A-mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pB-Tet-On-Luciferase-P2A-H2A-mCherry (JDW 1154)

    Plasmid

    #229840

    Purpose

    A Piggybac compatible, tet-on, dual reporter encoding luciferase followed by a P2A cleavage peptide and then an H2A fused mCherry for nuclear labeling.

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    Luciferase
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pdnCas3-CDA1

    Plasmid

    #229535

    Purpose

    pMV_hyg encoding dnCas3(H74A+D75A)-CDA1 fusion construct and crRNA expression cassette. Respective crRNA spacer can be introduced via BsmBI restriction cloning

    Depositor

    Kevin Verstrepen

    Article

    Zimmermann et al Nat Commun. 2023 Jun 9;14(1):3389. doi:

    Inserts

    dnCas3
    Cytidine deaminase
    Uracil glycosylase inhibitor
    

    Use

    Tags

    XTEN

    Expression

    Bacterial and Yeast

    Mutation

    codon optimized for S.cerevisiae and codon optimi…

    Promoter

    Available Since

    Jan. 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pdnCas3-APOBEC1

    Plasmid

    #229537

    Purpose

    pMV_hyg encoding dnCas3(H74A+D75A)-rAPOBEC1 fusion construct and crRNA expression cassette. Respective crRNA spacer can be introduced via BsmBI restriction cloning

    Depositor

    Kevin Verstrepen

    Article

    Zimmermann et al Nat Commun. 2023 Jun 9;14(1):3389. doi:

    Inserts

    rAPOBEC1
    dnCas3
    Uracil glycosylase inhibitor
    

    Use

    Tags

    XTEN

    Expression

    Bacterial and Yeast

    Mutation

    codon optimized for S.cerevisiae and codon optimi…

    Promoter

    Available Since

    Jan. 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pUC19-π-EB1_IRES-mCherry

    Plasmid

    #197427

    Purpose

    Homology-directed repair template targeting IRES-based π-element to MAPRE1 exon 5

    Depositor

    Torsten Wittmann

    Article

    Dema et al Elife. 2023 Jan 30;12:e84143. doi: 10.75

    Insert

    IRES-based π-element with HDR templates flanking MAPRE1 exon 5 insertion site (MAPRE1 Human)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    GCN4 leucine zipper, LOV2, Zdk1, and mCherry

    Expression

    Mutation

    Promoter

    Available Since

    June 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pUC19-π-EB1_EF1α-mCherry

    Plasmid

    #197429

    Purpose

    Homology-directed repair template targeting EF1α-based π-element to MAPRE1 exon 5

    Depositor

    Torsten Wittmann

    Article

    Dema et al Elife. 2023 Jan 30;12:e84143. doi: 10.75

    Insert

    EF1α-based π-element with HDR templates flanking MAPRE1 exon 5 insertion site (MAPRE1 Human)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    GCN4 leucine zipper, LOV2, Zdk1, and mCherry

    Expression

    Mutation

    Promoter

    Available Since

    June 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pUC19-π-EB1_EF1α-EGFP

    Plasmid

    #197430

    Purpose

    Homology-directed repair template targeting EF1α-based π-element to MAPRE1 exon 5

    Depositor

    Torsten Wittmann

    Article

    Dema et al Elife. 2023 Jan 30;12:e84143. doi: 10.75

    Insert

    EF1α-based π-element with HDR templates flanking MAPRE1 exon 5 insertion site (MAPRE1 Human)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    EGFP, GCN4 leucine zipper, LOV2, and Zdk1

    Expression

    Mutation

    Promoter

    Available Since

    June 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pJET-col2a1a-KI-donor

    Plasmid

    #184876

    Purpose

    Donor template for knock-in of p2a-CreERT2 into the medaka col2a1a locus via CRISPR/Cas9-mediated homology directed repair

    Depositor

    Christoph Winkler

    Article

    Hui et al Development. 2022 May 20. pii: 275483. d

    Inserts

    Collagen2a1a 5' homology arm
    Collagen2a1a 3' homology arm
    p2a-CreERT2
    Myosin light chain 2 promoter
    EGFP
    

    Use

    CRISPR and Cre/Lox

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    June 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pSMT3-FBF2 SS/Y

    Plasmid

    #166973

    Purpose

    Expresses FBF-2 with SS/Y mutations in repeat 5 in E. coli for purification and crystallization studies

    Depositor

    Traci Hall

    Article

    Bhat et al Elife. 2019 Jan 17;8. pii: 43788. doi: 1

    Insert

    FBF-2 (fbf-2 Nematode)
    

    Use

    Tags

    His-SUMO

    Expression

    Bacterial

    Mutation

    FBF-2 PUM RNA binding domain; amino acids 164-575…

    Promoter

    T7

    Available Since

    April 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pSMT3-FBF2 AS/Y

    Plasmid

    #166974

    Purpose

    Expresses FBF-2 with AS/Y mutations in repeat 5 in E. coli for purification and crystallization studies

    Depositor

    Traci Hall

    Article

    Bhat et al Elife. 2019 Jan 17;8. pii: 43788. doi: 1

    Insert

    FBF-2 (fbf-2 Nematode)
    

    Use

    Tags

    His-SUMO

    Expression

    Bacterial

    Mutation

    FBF-2 PUM RNA binding domain; amino acids 164-575…

    Promoter

    T7

    Available Since

    April 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pHR-HOXD13(+7A)-IDR-YFP-NLS

    Plasmid

    #145278

    Purpose

    Mammalian Expression of Nuclear HOXD13 (+7A) IDR-YFP with SV40 NLS

    Depositor

    Denes Hnisz

    Article

    Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

    Insert

    HOXD13(+7A)-IDR (HOXD13 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    A-repeat expanded

    Promoter

    Available Since

    Sept. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pET-45b-HOXA13(+7A)-IDR-mCherry

    Plasmid

    #145285

    Purpose

    Bacterial Expression of 6xHis HOXA13(+7A)IDR mCherry Fusion Protein

    Depositor

    Denes Hnisz

    Article

    Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

    Insert

    HOXA13(+7A)-IDR (HOXA13 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    A-repeat expanded

    Promoter

    Available Since

    Sept. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pCFP-LacI-HOXD13(+10A)-IDR

    Plasmid

    #145257

    Purpose

    Expresses fusion of ECFP-LacI and HOXD13+10A IDR

    Depositor

    Denes Hnisz

    Article

    Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

    Insert

    HOXD13(+10A)-IDR (HOXD13 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    A-repeat expanded

    Promoter

    Available Since

    Sept. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pCFP-LacI-HOXD13(+7A)-IDR

    Plasmid

    #145256

    Purpose

    Expresses fusion of ECFP-LacI and HOXD13+7A IDR

    Depositor

    Denes Hnisz

    Article

    Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

    Insert

    HOXD13(+7A)-IDR (HOXD13 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    A-repeat expanded

    Promoter

    Available Since

    Sept. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pGAL4-DBD-HOXD11-Adel-IDR

    Plasmid

    #145241

    Purpose

    Expresses fusion of GAL4 DNA-binding domain and HOXD11-Adel IDR

    Depositor

    Denes Hnisz

    Article

    Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

    Insert

    HOXD11-Adel-IDR (HOXD11 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    A-repeats deleted

    Promoter

    Available Since

    Sept. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pGAL4-DBD-HOXA13+7A-IDR

    Plasmid

    #145239

    Purpose

    Expresses fusion of GAL4 DNA-binding domain and HOXA13+7A IDR

    Depositor

    Denes Hnisz

    Article

    Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

    Insert

    HOXA13+7A-IDR (HOXA13 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    A-repeat expanded

    Promoter

    Available Since

    Sept. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pGAL4-DBD-EOMES-Adel-IDR

    Plasmid

    #145230

    Purpose

    Expresses fusion of GAL4 DNA-binding domain and EOMES-Adel IDR

    Depositor

    Denes Hnisz

    Article

    Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

    Insert

    EOMES-Adel-IDR (EOMES Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    A-repeats deleted

    Promoter

    Available Since

    Sept. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pGAL4-DBD-GSX2-Adel-IDR

    Plasmid

    #145232

    Purpose

    Expresses fusion of GAL4 DNA-binding domain and GSX2-Adel IDR

    Depositor

    Denes Hnisz

    Article

    Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

    Insert

    GSX2-Adel-IDR (GSX2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    A-repeats deleted

    Promoter

    Available Since

    Sept. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pFUW-tetO-KLF4

    Plasmid

    #139828

    Purpose

    doxycycline-inducible expression of Human KLF4 in mammalian cells

    Depositor

    Filipe Pereira

    Article

    Rosa et al Sci Immunol. 2018 Dec 7;3(30). pii: 3/30

    Insert

    KLF4 (KLF4 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE-mCMV

    Available Since

    April 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. U6_sgRNA_CAG_hSt1Cas9_MTH17CL396

    Plasmid

    #136656

    Purpose

    A single vector mammalian expression system containing a CAG promoter with Cas9 from Streptococcus thermophilus CRISPR1 (St1Cas9 LMD9:MTH17CL396 chimera) and its U6-driven sgRNA.

    Depositor

    Yannick Doyon

    Article

    Agudelo et al Genome Res. 2020 Jan;30(1):107-117. doi:

    Inserts

    St1Cas9 LMD-9:MTH17CL396 Chimera
    sgRNA for St1Cas9
    

    Use

    CRISPR and Synthetic Biology

    Tags

    SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CAG and hU6

    Available Since

    March 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. U6_sgRNA_CAG_hSt1Cas9_TH1477

    Plasmid

    #136655

    Purpose

    A single vector mammalian expression system containing a CAG promoter with Cas9 from Streptococcus thermophilus CRISPR1 (St1Cas9 LMD9:TH1477 chimera) and its U6-driven sgRNA.

    Depositor

    Yannick Doyon

    Article

    Agudelo et al Genome Res. 2020 Jan;30(1):107-117. doi:

    Inserts

    St1Cas9 LMD-9:TH1477 Chimera
    sgRNA for St1Cas9
    

    Use

    CRISPR and Synthetic Biology

    Tags

    SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CAG and hU6

    Available Since

    Feb. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge