174,131 results
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pcDNA3.1his p66shc S36A
Plasmid#10973DepositorAvailable SinceFeb. 14, 2006AvailabilityAcademic Institutions and Nonprofits only -
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Rc/CMV cyclin C
Plasmid#8961DepositorInsertcyclin C (CCNC Human)
ExpressionMammalianAvailable SinceJan. 5, 2006AvailabilityAcademic Institutions and Nonprofits only -
pSUPER.PKD1.RNAi
Plasmid#10815DepositorAvailable SinceDec. 7, 2005AvailabilityAcademic Institutions and Nonprofits only -
HA.PKD.S738A/S742A
Plasmid#10814DepositorInsertPKD (PRKD1 Human)
TagsHAExpressionMammalianMutationActivation loop inactive: S738A and S742A; L520PPromoterCMVAvailable SinceDec. 7, 2005AvailabilityAcademic Institutions and Nonprofits only -
pDY1330 STITCHR pCMV-nCas9-XTEN-v170_R2Tocc
Plasmid#234826PurposeSTITCHR R2Tocc editorDepositorInsertnCas9h840a-xten-R2Tocc
UseCRISPRAvailable SinceApril 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCMV_ENVLPE+
Plasmid#232427PurposeExpression plasmid to produce ENVLPE+ particlesDepositorInsertENVLPE+
ExpressionMammalianPromoter2xCMVAvailable SinceApril 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLenti_PE7-2A-BSD
Plasmid#234073PurposeFor lentiviral expression of PE7 with blasticidin resistanceDepositorInsertPE7
UseCRISPR and LentiviralTagsSV40 bpNLS and c-Myc NLSExpressionMammalianPromoterEF-1α core promoterAvailable SinceMarch 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
CROPseq-multi-Puro (pRW1441)
Plasmid#216217PurposeEntry vector to clone sgRNA(s) into the CROPseq-multi construct with Puromycin resistanceDepositorTypeEmpty backboneUseCRISPR and LentiviralExpressionMammalianAvailable SinceMarch 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
SPLICS LY-MT Long P2A
Plasmid#213613PurposeDetect the long-range Lysosome-Mitochondria contactDepositorInsertSPLICS LY-MT Long P2A
ExpressionMammalianAvailable SinceFeb. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJAM1.19_PB-CIT-PGK-Blast
Plasmid#205564PurposeExpress human CITDepositorAvailable SinceSept. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRRL.SIN.EF1A.CD19-FMC63.218.CAR-3G
Plasmid#194458PurposeCAR featuring: GMCSF (sig. peptide); CD8A & CD8A (hinge & TM); CD28, 4-1BB & CD3ζ (signaling domains); anti-CD19 from FMC63 in VL-VH order and 218 linker (scFv). GFP-Zeo for selection and monitoring.DepositorInsertAnti-CD19 CAR
UseLentiviralAvailable SinceMarch 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
psPax2-D64V-NC-mNeon
Plasmid#196509PurposemNeon fluorescent protein fused to nucleocapsid protein in psPAX2DepositorInsertmNeon
TagsmNeonExpressionMammalianPromoterCMVAvailable SinceMarch 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
SpCas9 TadCBEd-V106W
Plasmid#193839PurposeExpress TadCBEd-V106W (with SpCas9) in mammalian cellsDepositorInsertTadA-CDd V106W-SpCas9 D10A-2xUGI
ExpressionMammalianAvailable SinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9_T2A_mCherry sgAAVS1
Plasmid#192480PurposeAll-in-one plasmid for cutting non-targeting locus in DSB Spectrum V1 or V2 cells as controlDepositorInsertSpCas9
TagsT2A mCherryExpressionMammalianAvailable SinceDec. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
SspBmicro-mCh-p60
Plasmid#190168PurposeExpresses SspBmicro-mCh-p60, a recruitable katanin for microtubule severing, in mammalian cells.DepositorInsertSspBmicro-mCherry-p60
TagsSspBmicro-mCherryExpressionMammalianAvailable SinceDec. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pB025
Plasmid#183092PurposeExpresses FnCas12a in Bacteroides and used for genome editingDepositorInsertFnCas12a, gRNA, Promoter, HAB, TetR,
UseCRISPR and Synthetic BiologyExpressionBacterialPromoterP1TDPGH023Available SinceAug. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3/er-(n2)oxStayGold(c4)
Plasmid#185822PurposeA soluble photostable fluorescent marker of the ER lumen. The StayGold gene has no codon optimization for mammalian expression.DepositorInserter-(n2)oxStayGold(c4)
ExpressionMammalianPromoterCMVAvailable SinceJuly 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKO403_lacZ_Cm
Plasmid#174725PurposeBifidobacterium-E.coli shuttle vector for seamless gene cloningDepositorTypeEmpty backboneExpressionBacterialAvailable SinceJune 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLIX403_control_APOBEC_HA_P2A_mRuby_Capture1
Plasmid#183951PurposeInducible lentiviral expression, TRE-control-APOBEC-HA-P2A-mRuby; PGK-puro-2A-rtTADepositorAvailable SinceJune 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-ABE8e-nSpCas9-P2A-EGFP (KAC978)
Plasmid#185910PurposepCMV and pT7 plasmid encoding human codon optimized ABE8e A-to-G base editor with nickase SpCas9(D10A) and P2A-EGFPDepositorInserthuman codon optimized ABE8e-nSpCas9-P2A-EGFP
UseIn vitro transcription; t7 promoterTagsBPNLS and BPNLS-3xFLAG-P2A-EGFPExpressionMammalianMutationnSpCas9=D10APromoterCMV and T7Available SinceJune 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
GalT-RpHLuorin2
Plasmid#171719PurposeTrans-Golgi expression of ratiometric pHLuorin2DepositorInsertRatiometric pHLuorin2 fused to B4GALT1 (B4GALT1 Human)
Tagsratiometric pHluorin2ExpressionMammalianPromoterCMVAvailable SinceMarch 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGL4.10/RSV_SF3B3-miniXon_Luciferase
Plasmid#174660PurposePlasmid containing the SF3B3-miniXon/Firefly luciferase cassette. Translation of Firefly luciferase is controlled by splicing modulation of the SF3B3-miniXon by LMI070.DepositorInsertminiXon cassette
UseLuciferaseExpressionMammalianMutationKozak ATG initiation sequence in middle exonPromoterRSVAvailable SinceJan. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
mito-meGFP
Plasmid#172481PurposemeGFP targeted to the mitochondrial matrix with a human COX8 presequenceDepositorInsertMito
TagsmeGFPExpressionMammalianPromoterCMVAvailable SinceOct. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pETDuet S2
Plasmid#171921PurposeExpress RNA-based (p)ppGpp sensor and a NirFPDepositorInsertsRNA-based (p)ppGpp sensor
NirFP
ExpressionBacterialPromoterT7Available SinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.3_CoV2_D18
Plasmid#170442PurposeExpress SARS-CoV-2 WT spike protein with an 18aa deletion on the C-terminal tail. Used for SARS2 pseudotyped virus production.DepositorInsertSARS-CoV-2 spike D18 (S SARS-CoV-2, USA-WA1/2020 strain)
TagsNAExpressionMammalianMutation18aa deletion in c-terminal tailPromoterCMVAvailable SinceJuly 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG-Synapto-pHluorin
Plasmid#168510PurposeExpression of Synapto-pHluorin for optical monitoring of presynaptic release in neurons.DepositorAvailable SinceApril 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
MSCV-Cas9-2A-BFP
Plasmid#164662PurposeRetroviral introduction of Cas9 into mammalian cell line coupled to expression of blue fluorescent proteinDepositorInsertsCas9
BFP
UseCRISPR and RetroviralExpressionMammalianPromoterMSSV_LTRAvailable SinceMarch 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEG BacMam N term His8 eGFP 3C
Plasmid#160684PurposeVector optimized for use in screening assays, as well as for efficient production of baculovirus and robust expression of the target proteinDepositorTypeEmpty backboneTagsHis8 eGFP 3CExpressionMammalianPromoterCMVAvailable SinceDec. 9, 2020AvailabilityAcademic Institutions and Nonprofits only
