We narrowed to 6,070 results for: nls

1. pRvACT-mEGFP-NLS

   Plasmid

   #205001

   Purpose

   The vector contains 1kbp of the upstream and downstream regions of the actin gene from Ramazzottius varieornatus, with the mEGFP gene with NLS sequence positioned in between.

   Depositor

   Kazuharu Arakawa

   Article

   Tanaka et al Proc Natl Acad Sci U S A. 2023 Jan 31;12

   Insert

   mEGFP-NLS flanked by 1kbp upstream and downstream of the actin gene from Ramazzottius varieornatus
   

   Use

   Tardigrade expression

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. p3xP3-EGFP.vas-int.NLS

   Plasmid

   #60948

   Purpose

   Drosophila, p3xP3-EGFP.vas-int.NLS.attB was digested SpeI to get rid of the attB site. used for RMCE. CRISPR.

   Depositor

   Frank Schnorrer

   Article

   Zhang et al G3 (Bethesda). 2014 Oct 15. pii: g3.114.

   Insert

   vas-phiC31 integrase.NLS
   

   Use

   Rmce

   Tags

   Expression

   Mutation

   Promoter

   vasa

   Available since

   Nov. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

3. pQC NLS YFP IX

   Plasmid

   #37341

   Depositor

   Connie Cepko

   Article

   Beier et al Dev Biol. 2011 May 15;353(2):309-20. Epu

   Insert

   NLS YFP
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 27, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTetOne-DsRed-cNLS

   Plasmid

   #138528

   Purpose

   Inducible expression of DsRed-Express2 with nuclear localization signal

   Depositor

   Christopher Grigsby, Ana Teixeira

   Article

   Taylor-Weiner et al Proc Natl Acad Sci U S A. 2020 Feb 11;11

   Insert

   DsRed-Express2
   

   Use

   Tags

   3x SV40 cNLS

   Expression

   Mammalian

   Mutation

   Promoter

   TRE3Gs

   Available since

   April 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHRdSV40_scFv_GCN4_sfGFP-VPR-GB1_NLS

   Plasmid

   #79373

   Purpose

   Recruits VPR to a compatible Cas9 protein for transcriptional activation

   Depositor

   George Church

   Article

   Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

   Insert

   GCN4-sfGFP-VPR
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. NLS-HA-2xMCP-KRAB

   Plasmid

   #126589

   Purpose

   Expresses MCP (MS2 Coat Protein) fusion to KRAB in mammalian cells, lentiviral backbone

   Depositor

   David Segal

   Article

   O'Geen et al Epigenetics Chromatin. 2019 May 3;12(1):

   Insert

   2XMCP-KRAB
   

   Use

   Lentiviral

   Tags

   NLS-HA

   Expression

   Mammalian

   Mutation

   Promoter

   human ubiquitin C promoter

   Available since

   May 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pVITRO1-BFP-NLS

   Plasmid

   #184458

   Purpose

   Nucleus-targeted EBFP2 expression in mammalian cells

   Depositor

   Chuan-Hsiang Huang

   Article

   Yang et al Cell. 2021 Dec 9;184(25):6193-6206.e14.

   Insert

   EBFP2-3xNLS
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   Rat EF1α

   Available since

   May 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. NLS-LgBiT-CaM

   Plasmid

   #183401

   Purpose

   Expresses nuclear-localized LgBiT-CaM

   Depositor

   Alice Ting

   Article

   Erdenee et al ACS Synth Biol. 2022 Mar 18;11(3):1086-1

   Insert

   LgBiT-CaM
   

   Use

   Tags

   SV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. AsCas12a-2C-NLS in pCSDest

   Plasmid

   #126637

   Purpose

   Expresses AsCas12a-2C-NLS in mammalian cells

   Depositor

   Scot Wolfe

   Article

   Liu et al Nucleic Acids Res. 2019 Mar 20. pii: 540

   Insert

   AsCas12a-2C-NLS
   

   Use

   CRISPR

   Tags

   3xHuman influenza hemagglutinin (HA)-Tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV IE94 promoter

   Available since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pME-NLStdMCP- tagRFP

    Plasmid

    #86244

    Purpose

    Middle entry vector for inserting promotors for tissue specific expression of NLS-tdMCP-tagRFP

    Depositor

    Florence Marlow

    Article

    Campbell et al Development. 2015 Apr 1;142(7):1368-74.

    Type

    Empty backbone

    Use

    Unspecified

    Tags

    NLS-tdMCP-tagRFP

    Expression

    Mutation

    Promoter

    Available since

    Jan. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pQUASp-RedStinger (nlsRFP)

    Plasmid

    #46165

    Purpose

    Expression of RedStinger in insect

    Depositor

    Christopher Potter

    Type

    Empty backbone

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Aug. 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. NLS-4LNDDE488Q-pCDNA3.1(+)

    Plasmid

    #172314

    Purpose

    Expresses ADAR2 Deaminase Domain (DD) hyperactive mutant E488Q fused with 4 repeats of lambda N peptide (4LN) with a Nuclear Localization Signal (NLS)

    Depositor

    Joshua Rosenthal

    Article

    Vallecillo-Viejo et al RNA Biol. 2018 Jan 2;15(1):104-114. doi:

    Insert

    Lambda N peptide - human ADAR2 Deaminase Domain E488Q
    

    Use

    Tags

    6xHis, Flag, and Nuclear Localization Signal of S…

    Expression

    Mammalian

    Mutation

    changed the Glutamic acid 488 in the Deaminase Do…

    Promoter

    CMV

    Available since

    Aug. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3-AktAR2-NLS

    Plasmid

    #175012

    Purpose

    Nuclear-targeted AktAR2.

    Depositor

    Jin Zhang

    Article

    Zhou et al Nat Commun. 2020 Nov 30;11(1):6088. doi:

    Insert

    AktAR-NLS
    

    Use

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. NLS-HA-tdMCP-tdTomato

    Plasmid

    #183938

    Purpose

    tandem MS2 binding protein labeled with tdTomato, containing a NLS and a HA-tag

    Depositor

    Karsten Rippe

    Article

    Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22

    Insert

    tdMCP-tdTomato (cp Synthetic)
    

    Use

    Tags

    NLS-HA

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV (TATA box removed)

    Available since

    June 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pME-nlsGFP-P2A

    Plasmid

    #80808

    Purpose

    nuclear localization signal fused to N-terminus of GFP (nlsGFP) without stop codon, followed by P2A

    Depositor

    Philip Washbourne

    Article

    Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

    Insert

    minimal cytomegalovirus immediate early enhancer/promoter
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. plenti-As-Cas12a-2xNLS

    Plasmid

    #155047

    Purpose

    Lentiviral vector expressing human codon-optimized _C-terminal Myc-tagged _Acidaminococcus sp. BV3L6 (As)-Cas12a nuclease with N- and C-terminal NLS and Neomycin/G418/Geneticin resistance from EF-1alpha promoter

    Depositor

    Jason Moffat, Benjamin Blencowe

    Article

    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

    Insert

    AsCas12a
    

    Use

    CRISPR and Lentiviral

    Tags

    Nucleoplasmin NLS, Myc-tag and SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a

    Available since

    Aug. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. mEGFP-PCNA-19-SV40NLS-4

    Plasmid

    #56469

    Purpose

    Localization: Nucleus, Excitation: 488, Emission: 507

    Depositor

    Michael Davidson

    Insert

    PCNA-19 SV40NLS (PCNA Human)
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 1, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

18. pMEH275 NLS dTomato

    Plasmid

    #168210

    Purpose

    Mammalian expression of nuclear-localized dTomato

    Depositor

    Joshua Leonard

    Article

    Hung et al J Extracell Vesicles. 2016 May 13;5:3102

    Insert

    NLS dTomato
    

    Use

    Lentiviral

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCFP-LacI-NLS

    Plasmid

    #103840

    Purpose

    Flourescent marker for lacO arrays

    Depositor

    Karsten Rippe

    Article

    Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

    Insert

    CFP-LacI-NLS
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    LacI: C19T (silent, L7L), T264C (silent, A88A), C…

    Promoter

    SV2

    Available since

    Jan. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pQC NLS mPlum IX

    Plasmid

    #37356

    Depositor

    Connie Cepko

    Article

    Beier et al Dev Biol. 2011 May 15;353(2):309-20. Epu

    Insert

    NLS mPlum
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 30, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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