20,141 results

Showing: 9871 - 9900 of 20141 results

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pEX-CFP-hLC3WT

Plasmid

#24985

Depositor

Isei Tanida

Article

Tanida et al Autophagy. 2008 Jan 1. 4(1):131-4.

Insert

a wild type human MAP1-LC3B fused with ECFP (MAP1LC3B Human)

Tags

ECFP

Expression

Mammalian

Available Since

June 21, 2010

Availability

Academic Institutions and Nonprofits only

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AnkyrinG-190-GFP

Plasmid

#31059

Depositor

Vann Bennett

Article

Mohler et al J Biol Chem. 2002 Mar 22. 277(12):10599-

Insert

Ankyrin G (Ank3 Rat)

Tags

EGFP

Expression

Mammalian

Available Since

Feb. 28, 2012

Availability

Academic Institutions and Nonprofits only

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pYPQ143

Plasmid

#69295

Purpose

Golden Gate recipient and Gateway entry vector; assembly of 3 gRNAs

Depositor

Yiping Qi

Article

Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

Type

Empty backbone

Use

CRISPR

Available Since

Nov. 4, 2015

Availability

Academic Institutions and Nonprofits only

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GFP shRNA tet pLKO puro

Plasmid

#163017

Purpose

Control tet-inducible shRNA targeting eGFP

Depositor

Mark Rubin

Article

Cotter et al Mol Cancer Res. 2021 Jun 4. pii: 1541-77

Insert

Enhanced Green Fluorescent Protein

Use

Lentiviral

Available Since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

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FUS-Citrine-PixE

Plasmid

#111505

Purpose

For PixELL system

Depositor

Jared Toettcher

Article

Dine et al Cell Syst. 2018 May 24. pii: S2405-4712(

Insert

PixE (slr1693 )

Use

Lentiviral

Tags

Citrine and FUS

Promoter

SFFV

Available Since

June 14, 2018

Availability

Academic Institutions and Nonprofits only

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SAP145

Plasmid

#11239

Depositor

Robin Reed

Article

Gozani et al Genes Dev. 1996 Jan 15. 10(2):233-43.

Insert

SAP145 (SF3B2 Human)

Expression

Bacterial

Mutation

First 7 amino acids are missing compared to NP_00…

Available Since

Jan. 19, 2006

Availability

Academic Institutions and Nonprofits only

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pNCS-CyOFP

Plasmid

#74278

Purpose

Constitutive expression of CyOFP in bacteria

Depositor

Michael Lin

Article

Chu et al Nat Biotechnol. 2016 May 30. doi: 10.103

Insert

CyOFP

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

March 29, 2016

Availability

Academic Institutions and Nonprofits only

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AAV-hSyn-Soma-jGCaMP8m

Plasmid

#169257

Purpose

AAV transfer plasmid for neuronal expression of soma-targeted jGCaMP8m.

Depositor

Marianne Fyhn

Article

Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1

Has Service

AAV Retrograde, AAV1, and AAV9

Insert

Soma-jGCaMP8m

Use

AAV

Tags

6xHis-tag

Expression

Mammalian

Available Since

May 18, 2021

Availability

Academic Institutions and Nonprofits only

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BcatMutS33/S37.T41/S45

Plasmid

#24204

Depositor

David Rimm

Insert

beta catenin (CTNNB1 Human)

Tags

FLAG

Expression

Mammalian

Mutation

S33A, S37A, T41A,S45A

Promoter

CMV

Available Since

March 9, 2010

Availability

Academic Institutions and Nonprofits only

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p3868 HA-E6-AP

Plasmid

#8648

Depositor

Peter Howley

Article

Talis et al J Biol Chem 1998 Mar 13;273(11):6439-45.

Insert

E6-AP (UBE3A Human)

Tags

HA

Expression

Mammalian

Available Since

Oct. 11, 2005

Availability

Academic Institutions and Nonprofits only

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pPR219.05

Plasmid

#78554

Purpose

Plasmid expressing UirR, response regulator and PcsiR1-regulated GFP output

Depositor

Jeffrey Tabor

Article

Ramakrishnan et al ACS Synth Biol. 2016 Apr 27.

Insert

uirR

Use

Synthetic Biology

Promoter

J23115

Available Since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV.GFAP.(Cyto)iGlucoSnFR

Plasmid

#164513

Purpose

Green glucose sensor, cytosolic. The depositing lab recommends the updated pAAV.GFAP.(cyto).iGlucoSnFR2 (Plasmid #244111) instead of this plasmid.

Depositor

Loren Looger

Article

Keller et al Cell Rep. 2021 Jun 22;35(12):109284. doi

Insert

(Cyto)iGlucoSnFR

Use

AAV

Mutation

Synthetic Construct

Promoter

GFAP

Available Since

Feb. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pBa-KIF5C 559-tdTomato-FKBP

Plasmid

#64211

Purpose

KIF5C motor protein aa1-559 with C-terminal tandem dimer Tomato and FKBP tags

Depositor

Gary Banker, Marvin Bentley

Article

Bentley et al J Cell Biol. 2015 Feb 2;208(3):273-281.

Insert

Kif5c (Kif5c Rat)

Tags

FKBP and tdTomato

Expression

Mammalian

Mutation

motor domain aa1-559

Promoter

chicken Beta Actin

Available Since

June 17, 2015

Availability

Academic Institutions and Nonprofits only

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TriEx-4 DHX36

Plasmid

#68368

Purpose

Expresses the N-terminal histidine-tagged DXH36 gene product upon transfection into Rosetta 2 E. coli and induction with IPTG.

Depositor

James Vaughn

Article

Vaughn et al J Biol Chem. 2005 Nov 18;280(46):38117-2

Insert

DHX36 (DHX36 Human)

Tags

Histidine

Expression

Bacterial, Insect, and Mamm…

Promoter

CMV ie enhancer/promoter

Available Since

Sept. 29, 2016

Availability

Academic Institutions and Nonprofits only
pMaCTag-P07

Plasmid

#124788

Purpose

Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with mNeonGreen

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

mNeonGreen

Use

Pcr template

Tags

mNeonGreen

Promoter

None

Available Since

May 7, 2019

Availability

Academic Institutions and Nonprofits only

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TRX1 flag C35S

Plasmid

#21285

Depositor

Wang Min

Article

Liu et al Circ Res. 2002 Jun 28. 90(12):1259-66.

Insert

TRX1 (TXN Human)

Tags

Flag

Expression

Mammalian

Mutation

C35S

Available Since

July 30, 2009

Availability

Academic Institutions and Nonprofits only
pDONR223-MARK3

Plasmid

#23716

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

MARK3 (MARK3 Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pSuperRetropuro-IL-6 scramble

Plasmid

#19960

Depositor

Christopher Counter

Article

Ancrile et al Genes Dev. 2007 Jul 15. 21(14):1714-9.

Insert

pSuperRetropuro-IL-6 scramble

Use

RNAi and Retroviral

Available Since

Jan. 12, 2009

Availability

Academic Institutions and Nonprofits only
pcDNA6-N-Flag-SREBP2

Plasmid

#134284

Purpose

Expresses Flag-tagged SREBP2 in mammalian cells

Depositor

Bruce Beutler

Article

Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

Insert

SREBP2 (Srebf2 Mouse)

Tags

Flag

Expression

Mammalian

Mutation

mouse SREBP2 precursor

Promoter

CMV

Available Since

March 20, 2020

Availability

Academic Institutions and Nonprofits only

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pmCherry-TPX2

Plasmid

#31227

Depositor

Patricia Wadsworth

Article

Ma et al Mol Biol Cell. 2010 Mar 15. 21(6):979-88

Insert

TPX2 (TPX2 Human)

Tags

mCherry

Expression

Mammalian

Available Since

July 29, 2011

Availability

Academic Institutions and Nonprofits only

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pWZL Neo Myr Flag PKM2

Plasmid

#20585

Depositor

William Hahn, Jean Zhao

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

PKM2 (PKM Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Available Since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only

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pAAV-U6-pegSERPINA1-U6-Nicking-PE2-N

Plasmid

#164907

Purpose

Express a pegRNA targeting SERPINA1, and a nicking sgRNA and the N-terminal split-intein fragment of PE2

Depositor

Wen Xue

Article

Liu et al Nat Commun. 2021 Apr 9;12(1):2121. doi:

Insert

pegSERPINA1, Nicking sgRNA, PE2-N

Use

AAV

Available Since

Feb. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pMJ825

Plasmid

#39315

Depositor

Jennifer Doudna

Article

Jinek et al Science. 2012 Aug 17;337(6096):816-21. d

Insert

Cas9

Use

CRISPR

Tags

His6 and MBP

Expression

Bacterial

Mutation

Aspartate 10 to Alanine (D10A)

Promoter

T7

Available Since

Aug. 28, 2012

Availability

Academic Institutions and Nonprofits only

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pSQT1313

Plasmid

#53370

Purpose

multiplex gRNA expression plasmid

Depositor

Keith Joung

Article

Tsai et al Nat Biotechnol. 2014 Apr 25. doi: 10.103

Insert

None

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

June 19, 2014

Availability

Academic Institutions and Nonprofits only

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pmCherry Vinculin

Plasmid

#50527

Depositor

Kenneth Yamada

Insert

Vinculin (VCL Chicken)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA1 Flag ATXN1[30Q]

Plasmid

#33236

Depositor

Huda Zoghbi

Article

Skinner et al Nature. 1997 Oct 30;389(6654):971-4.

Insert

Ataxin-1 (ATXN1 Human)

Tags

Flag

Expression

Mammalian

Mutation

insert contains a stretch of 29 amino acids (27 Q…

Promoter

CMV

Available Since

July 18, 2014

Availability

Academic Institutions and Nonprofits only
pEGFP VAMP8

Plasmid

#42311

Depositor

Thierry Galli

Article

Paumet et al J Immunol. 2000 Jun 1;164(11):5850-7.

Insert

VAMP 8 (VAMP8 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

March 12, 2013

Availability

Academic Institutions and Nonprofits only

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mKate2-P2A-APEX2-GBP

Plasmid

#67650

Purpose

Mammalian expression vector driving APEX2-GBP (EM peroxidase marker fused to GFP-targeting nanobody). Also contains bicistronially expressed cytoplasmic red fluorescent reporter.

Depositor

Rob Parton

Article

Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

Insert

APEX2-GBP

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 30, 2015

Availability

Academic Institutions and Nonprofits only

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pMSCV mKeima-LC3

Plasmid

#189006

Purpose

Fluorescent reporter for autophagy

Depositor

Atsushi Hoshino

Article

Minami et al Nat Commun. 2023 Jul 13;14(1):4084. doi:

Insert

mKeima, LC3 (Map1lc3b Rat)

Use

Retroviral

Available Since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pRU294

Plasmid

#167689

Purpose

Final destination vector for single Gateway LR reaction

Depositor

Niko Geldner

Article

Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

Insert

pEC1.2::Cas9i

Use

CRISPR

Available Since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge

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20,141 results