We narrowed to 14,498 results for: SHR;

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pCFD5-frame_selector_0,1,2

Plasmid

#131152

Purpose

Drosophila expression of frame selector sgRNAs 0,1, and 2 that target the CRISPaint site for homology-independent knock-in

Depositor

Norbert Perrimon

Article

Bosch et al Genetics. 2019 Nov 4. pii: genetics.119.

Insert

CRISPaint frame selector sgRNAs 0,1,2

Use

CRISPR

Expression

Insect

Promoter

dU6:3

Available Since

Sept. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pX459-HypaCas9-mR2-AAVS1_sgRNA

Plasmid

#183890

Purpose

pX459V2.0-HypaCas9 plasmid with mRuby2 reporter and sgRNA targeting the AAVS1 in human cells.

Depositor

Alisa Piekny

Article

Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

Insert

AAVS1 sgRNA spacer (AAVS1 Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

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LentiCRISPR-V2_hMSH2

Plasmid

#186155

Purpose

sgRNA targeting human MSH2 gene

Depositor

David McFadden

Article

Povedano et al Cell Chem Biol. 2019 Sep 19;26(9):1315-1

Insert

MSH2 (MSH2 Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

July 6, 2022

Availability

Academic Institutions and Nonprofits only

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pDECKO_GFP

Plasmid

#72619

Purpose

Expresses two gRNAs targeting GFP

Depositor

Roderic Guigo, Rory Johnson

Article

Aparicio-Prat et al BMC Genomics. 2015 Oct 23;16(1):846. doi

Insert

gRNAs toward GFP

Expression

Mammalian

Promoter

U6 (gRNA1) and H1 (gRNA2)

Available Since

June 14, 2016

Availability

Academic Institutions and Nonprofits only

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pmCherry_sgRNA-ver2

Plasmid

#126776

Purpose

gRNA expression vector with mCherry transfection control, does not contain the direct repeat of the truncated guideRNA

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

Insert

sgRNA (for SpCas9)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

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pXPR_003 sgNFkB2 guide 1

Plasmid

#193593

Purpose

NFkB2 knockout

Depositor

William Hahn

Article

Hagel et al Cancer Res. 2022 Dec 22:CAN-22-2245. doi

Insert

sgNFkB2 guide 1 (NFKB2 Human)

Use

Lentiviral

Available Since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pUC19-U6-LwaCas13acrRNA-BsmBIcassette (LTH151)

Plasmid

#171129

Purpose

LwaCas13a crRNA entry vector for pU6 expression (need to clone in spacer into BsmBI sites)

Depositor

Benjamin Kleinstiver

Article

Johnson et al Mol Cell. 2022 Jun 2;82(11):2161-2166.e3

Insert

U6 promoter entry plasmid for LwaCas13a crRNA cloning

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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pLQ-Pxyl/tet-cas9-Pj23119-sgRNA

Plasmid

#92121

Purpose

CRISPR-Cas9 based efficient genome editing in S. aureus

Depositor

Sheng Yang

Article

Liu et al Acta Biochim Biophys Sin (Shanghai). 201

Insert

Cas9-Pxyltet-sgRNA-pj23119

Use

CRISPR

Expression

Bacterial

Available Since

Feb. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pFlag-CMV2-Brd4 del ET

Plasmid

#21938

Depositor

Eric Verdin

Article

Bisgrove et al Proc Natl Acad Sci U S A. 2007 Aug 21. 1

Insert

BRD4 (BRD4 Human)

Tags

Flag

Expression

Mammalian

Mutation

aa 1-1362 only, ET domain deleted

Available Since

Sept. 16, 2009

Availability

Academic Institutions and Nonprofits only
pV1326

Plasmid

#111435

Purpose

S. cerevisiae and C. glabrata Solo CRISPR vector, marked with URA3 and NatR

Depositor

Gerald Fink

Article

Vyas et al mSphere. 2018 Apr 25;3(2). pii: 3/2/e001

Insert

CaCas9/sgRNA-BsmBI stuffer

Use

CRISPR

Expression

Yeast

Available Since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

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SiC-V1-Scr

Plasmid

#133042

Purpose

SiC-V1 vector with scramble (Scr) sgRNA. This lentivirus construct is used for stably expressing spCas9 with a dTomato reporter in a cell line of interest.

Depositor

Sriram Neelamegham

Article

Kelkar et al Mol Ther. 2019 Sep 12. pii: S1525-0016(1

Insert

SpCas9

Use

CRISPR and Lentiviral

Tags

dTomato

Available Since

Nov. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pGTRm

Plasmid

#127197

Purpose

pGTRm is the template plasmid for PCR & golden gate assembly driven generation of polycistronic tRNA-gRNA sequences

Depositor

Jordan Green

Article

Rui et al Mol Ther Nucleic Acids. 2020 Apr 21;20:6

Insert

tRNA-gRNA PCR template

Use

Template plasmid for generation of golden gate pc…

Promoter

None

Available Since

May 28, 2020

Availability

Academic Institutions and Nonprofits only

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AAV-FLEX-EGFP-mir30(Scn9a)

Plasmid

#79672

Purpose

Cre-dependent knockdown of Scn9a (Nav1.7)

Depositor

Scott Sternson

Article

Branco et al Cell. 2016 Jun 16;165(7):1749-61. doi: 1

Insert

EGFP (Scn9a Mouse, jellyfish)

Use

AAV and Cre/Lox

Expression

Mammalian

Promoter

CAG

Available Since

July 27, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-GFP-mirNega

Plasmid

#163705

Purpose

pAAV plasmid to induce expression of universal negative control micro-RNA and EGFP

Depositor

Michisuke Yuzaki

Article

Ito-Ishida et al J Neurosci. 2020 Nov 4;40(45):8746-8766.

Insert

mirNega and EGFP

Use

AAV

Promoter

CAG

Available Since

March 17, 2021

Availability

Academic Institutions and Nonprofits only

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L4440_BioBrick-hif-1-sgRNA-B

Plasmid

#177785

Purpose

Overexpression of sgRNAs in E. coli HT115 (targeting C. elegans hif-1 promoter)

Depositor

Michael Ristow

Article

Fischer et al J Biol Chem. 2022 May 27:102085. doi: 10

Insert

hif-1 (hif-1 Nematode)

Expression

Bacterial

Available Since

June 1, 2022

Availability

Academic Institutions and Nonprofits only

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pSmart HC Kan-U6-Nla-repeat-BbsI-Nla-repeat

Plasmid

#178883

Purpose

Vector for cloning Nla-IC-CRISPR RNA for expression in mammalian cells.

Depositor

Yan Zhang

Article

Tan et al Mol Cell. 2022 Feb 17;82(4):852-867.e5.

Insert

U6-Nla repeat-BbsI-Nla repeat

Expression

Mammalian

Promoter

U6

Available Since

Jan. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pLV sgRNA-Hic1 GFP

Plasmid

#106953

Purpose

Lentivirus encoding sgRNA targeting murine Hic1. Includes GFP marker.

Depositor

Albert Edge

Article

Lenz et al Front Cell Dev Biol. 2019 Feb 19;7:14. d

Insert

anti-Hic1 sgRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Dec. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pORANGE GFP-Grin1 KI #3

Plasmid

#139659

Purpose

Endogenous tagging of GluN1: N-terminal (amino acid position: A20)

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

gRNA and GFP donor

Expression

Mammalian

Promoter

U6 and Cbh

Available Since

May 15, 2020

Availability

Academic Institutions and Nonprofits only

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pJE53

Plasmid

#194153

Purpose

sgRNA expression in A. baumannii for CRISPRi. Replicative plasmid, KmR. Contains mrfp nontargeting control guide sequence.

Depositor

Edward Geisinger

Article

Bai et al J Bacteriol. 2021 May 20;203(12):e005652

Insert

sgRNA with mrfp control guide sequence

Expression

Bacterial

Promoter

J23119

Available Since

Feb. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pQCascade

Plasmid

#140622

Purpose

Expresses V. cholerae TniQ, Cas8, Cas7, and Cas6 and CRISPR RNA

Depositor

Sheng Yang

Article

Zhang et al ACS Synth Biol. 2020 Jun 17. doi: 10.102

Insert

VchTniQ, VchCas8, VchCas7, VchCas6, CRISPR(entry)

Use

CRISPR; Transposon

Expression

Bacterial

Available Since

July 16, 2020

Availability

Academic Institutions and Nonprofits only

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