We narrowed to 59,739 results for: Gene

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pCR8-attL5-pegRNA-attR3

Plasmid

#213054

Purpose

Modular Unit carrying pegRNA and ngRNA scaffolds flanked by attL5 and attR3

Depositor

Bing Yang

Article

Gupta et al Plant Commun. 2023 Oct 26:100741. doi: 1

Insert

sgRNA

Expression

Bacterial

Available Since

Feb. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pCJH019_SC101_pLAC_Cas12c(4)_Kan

Plasmid

#183071

Purpose

Wild-type Cas12c_4 in a plasmid containing a SC101 origin and lac promoter for in vivo interference assay.

Depositor

Jennifer Doudna

Article

Huang et al Mol Cell. 2022 Jun 2;82(11):2148-2160.e4

Insert

Cas12c_4

Expression

Bacterial

Mutation

Wild-type

Available Since

June 2, 2022

Availability

Academic Institutions and Nonprofits only

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pMOD_D0000a

Plasmid

#127230

Purpose

Empty Backbone for golden gate cloning compatible with Cermak., et al Toolkit.

Depositor

Daniel Voytas

Article

Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

Type

Empty backbone

Expression

Plant

Available Since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pVS1-SAH2

Plasmid

#134746

Purpose

Replication helper

Depositor

Qi-Jun Chen

Article

Zhang et al Plant Physiol. 2019 Sep 26. pii: pp.19.0

Insert

trfA-trfB-parDE

Use

Helper plasmid

Available Since

Dec. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pCI-Myc3-HPS4

Plasmid

#133931

Purpose

Expresses 3xMyc-HPS4 construct in mammalian cells

Depositor

Juan Bonifacino

Article

Martina et al J Biol Chem. 2003 Aug 1;278(31):29376-84

Insert

HPS4 (HPS4 Human)

Tags

3xMyc tag

Expression

Mammalian

Mutation

Glutamic acid 229 to Glycine; Valine 552 to Methi…

Promoter

CMV I.E.

Available Since

Nov. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pBBR5pemIKpKan

Plasmid

#129271

Purpose

Broad host-range plasmid with toxin-antitoxin system for maintenance in the absence of antibiotic selection, and constitutive promoter

Depositor

Lindsey Burbank

Article

Burbank et al Microbiol Resour Announc. 2019 Sep 5;8(3

Type

Empty backbone

Expression

Bacterial

Available Since

Aug. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pCR8/GW_TOPO_Cdk12_Nterm_FlagHa

Plasmid

#127177

Purpose

N-terminal Flag- HA- tandem epitope tagged mouse Cdk12 transgene (NM_001109626.1 isoform) cloned into Gateway entry vector

Depositor

Phil Sharp

Article

Dubbury et al Nature. 2018 Dec;564(7734):141-145. doi:

Insert

N-terminally Flag- HA- Epitope Tagged Mouse Cyclin Dependent Kinase 12 (Cdk12 Mouse)

Use

Entry vector with transgene for gateway cloning

Tags

Flag- HA- Tandem Epitopes

Promoter

None

Available Since

July 5, 2019

Availability

Academic Institutions and Nonprofits only

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pBBR6k-GFPuv

Plasmid

#106384

Purpose

IPTG-inducible LlacO-1 promoter expressing gfpuv on BBR1 origin

Depositor

Brian Pfleger

Article

Cook et al J Ind Microbiol Biotechnol. 2018 Jan 3.

Insert

GFPuv

Expression

Bacterial

Promoter

LlacO-1

Available Since

March 22, 2018

Availability

Academic Institutions and Nonprofits only

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pLO87

Plasmid

#91726

Purpose

mycobacterial expression of mCherry from Hsp60 promoter

Depositor

Graham Hatfull

Article

Oldfield et al J Bacteriol. 2014 Oct;196(20):3589-97. d

Insert

hsp60 promoter

Use

Mycobacterial expression

Tags

mCherry

Promoter

hsp60

Available Since

May 18, 2017

Availability

Academic Institutions and Nonprofits only

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pICH47742:FCP:Cas9YFP

Plasmid

#85986

Purpose

Golden Gate Level 1 cassette encoding a Cas9-YFP fusion under the FCP promoter

Depositor

Thomas Mock

Article

Hopes et al Plant Methods. 2016 Nov 24;12:49. doi: 1

Insert

Cas9

Use

CRISPR; Golden gate assembly

Tags

SV40 NLS, YFP

Promoter

FCP

Available Since

Jan. 18, 2017

Availability

Academic Institutions and Nonprofits only

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pFUS_A2A

Plasmid

#43849

Depositor

Takashi Yamamoto

Article

Sakuma et al Genes Cells. 2013 Feb 6. doi: 10.1111/gt

Insert

LacZ + BsaI restriction sites

Available Since

March 28, 2013

Availability

Academic Institutions and Nonprofits only

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pBS-mHopX

Plasmid

#16425

Depositor

Jonathan Epstein

Article

Chen et al Cell. 2002 Sep 20. 110(6):713-23.

Insert

Hopx (hop Mouse)

Use

In situ template

Mutation

None

Available Since

Dec. 14, 2007

Availability

Academic Institutions and Nonprofits only

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-111 hTF m2

Plasmid

#15449

Depositor

Nigel Mackman

Article

Cui et al J Biol Chem. 1996 Feb 2. 271(5):2731-9.

Insert

Tissue factor promoter (F3 Human)

Use

Luciferase

Expression

Mammalian

Mutation

All 3 Sp1 sites mutated

Available Since

Aug. 31, 2007

Availability

Academic Institutions and Nonprofits only
pTRE3G-EGFP-IRES(Neo)R

Plasmid

#158068

Purpose

eGFP under the TRE3G promoter, Neo resistance. For cloning GOI

Depositor

Adolfo Rivero-Muller

Article

Michalec-Wawiorka et al Int J Mol Sci. 2021 May 14;22(10). pii:

Insert

eGFP

Tags

eGFP

Expression

Mammalian

Promoter

TRE3G

Available Since

Aug. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pH-ABE8e

Plasmid

#200774

Purpose

For plant adenine base editing in rice plants or monocotyledons protoplasts

Depositor

Caixia Gao

Article

Xue et al Nat Biotechnol. 2023 Mar 9. doi: 10.1038

Insert

nCas9(D10A)-TadA8e

Use

CRISPR

Expression

Plant

Mutation

D10A for Cas9; W23R, H36L, P48A, R51L, L84F, A106…

Promoter

maize Ubiquitin-1, OsU3

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pFUGW NLS-FlpO

Plasmid

#183448

Purpose

Lentiviral FlpO vector

Depositor

Harold MacGillavry

Article

Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

Insert

NLS-FlpO

Use

Lentiviral

Expression

Mammalian

Available Since

April 25, 2022

Availability

Academic Institutions and Nonprofits only

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pXL-BacII-CAG-mCherry

Plasmid

#170519

Purpose

Plasmid containing piggyBac ITRs flanking CAG and mCherry (also see doi: 10.7554/eLife.63907 )

Depositor

Joshua Sanes

Article

Yamagata et al Bio Protoc. 2021 Aug 5;11(15):e4105. doi

Insert

CAG-mCherry

Use

Piggybac transposon

Expression

Mammalian

Promoter

CAG

Available Since

Feb. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pTRE3G-EGFPC-uL10-(Neo)R

Plasmid

#158069

Purpose

eGFP and uL10 ribosomal protein under the TRE3G promoter, Neo resistance

Depositor

Adolfo Rivero-Muller

Article

Michalec-Wawiorka et al Int J Mol Sci. 2021 May 14;22(10). pii:

Insert

RPLP0 (RPLP0 Human)

Tags

eGFP

Expression

Mammalian

Promoter

TRE3G

Available Since

Nov. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pMSCVhyg-3xT7-CA-ChREBP

Plasmid

#171029

Purpose

Expresses 3xT7-tagged constitutive active form of ChREBP in mammalian cells

Depositor

Kai Ge

Article

Jang et al Genes Dev. 2021 Apr 22. pii: gad.347583.

Insert

CA-ChREBP (Mlxipl Mouse)

Use

Retroviral

Tags

3xT7

Expression

Mammalian

Mutation

amino acids 240 - 864

Promoter

5' LTR

Available Since

Aug. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pJMP2846

Plasmid

#160676

Purpose

Mobile CRISPRi empty sgRNA (BsaI) for cloning new sgRNAs

Depositor

Jason Peters

Article

Banta et al Curr Protoc Microbiol. 2020 Dec;59(1):e1

Type

Empty backbone

Expression

Bacterial

Available Since

Nov. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pASR103

Plasmid

#122637

Purpose

BglFusion compatible IPTG inducible integration vector for Streptococcus pneumoniae (pPEPZ Backbone)

Depositor

Jan-Willem Veening

Article

Keller et al Genes (Basel). 2019 May 22;10(5). pii: g

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

Aug. 16, 2019

Availability

Academic Institutions and Nonprofits only

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ABCG1_pCSdest

Plasmid

#53952

Depositor

Roger Reeves

Article

Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

Insert

ABCG1 (ABCG1 Human)

Expression

Mammalian

Promoter

SP6 and CMV

Available Since

March 27, 2018

Availability

Industry, Academic Institutions, and Nonprofits
MCM3APlong_pCSdest

Plasmid

#53940

Depositor

Roger Reeves

Article

Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

Insert

MCM3AP long (MCM3AP Human)

Expression

Mammalian

Promoter

SP6 and CMV

Available Since

March 27, 2018

Availability

Industry, Academic Institutions, and Nonprofits
Runx1_pCSdest

Plasmid

#53802

Depositor

Roger Reeves

Article

Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

Insert

Runx1 (RUNX1 Human)

Expression

Mammalian

Promoter

SP6 and CMV

Available Since

March 27, 2018

Availability

Industry, Academic Institutions, and Nonprofits
pHES841

Plasmid

#89194

Purpose

Expresses YFP under control of synthetic promoter containing Zif268 binding sites.

Depositor

Hana El-Samad

Article

Aranda-Diaz et al ACS Synth Biol. 2016 Dec 27. doi: 10.102

Insert

pZ-YFP

Expression

Yeast

Promoter

pZ

Available Since

Jan. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pNBU2_erm_P1T_DP-B1

Plasmid

#90319

Purpose

Integration vector at an attB site in Bacteroides; contains the P1T_DP aTC-inducible promoter with the RBS B1

Depositor

Andrew Goodman

Article

Lim et al Cell. 2017 Apr 20;169(3):547-558.e15. do

Type

Empty backbone

Expression

Bacterial

Available Since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

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pMD19T-slr0230-PL22-sgRNANT1-KmR

Plasmid

#73224

Purpose

Contains sgRNA which targets GFPmut3b. Under an aTc inducible promoter. Suicide vector inserts into slr0230 site of Synechocystis. Carries kanamycin resistance. Propogates in E. coli

Depositor

Paul Hudson

Article

Yao et al ACS Synth Biol. 2015 Dec 28.

Insert

PL22-sgRNA NT1

Expression

Bacterial

Promoter

PL22

Available Since

April 15, 2016

Availability

Academic Institutions and Nonprofits only

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Luc-Pten-3'UTR

Plasmid

#64098

Purpose

Pten Luciferase reporter

Depositor

Lin He

Article

Olive et al Genes Dev. 2009 Dec 15. 23(24):2839-49.

Insert

Pten 3'UTR (PTEN Human)

Expression

Mammalian

Available Since

June 24, 2015

Availability

Academic Institutions and Nonprofits only
MLS-mir-17-92

Plasmid

#64090

Purpose

expresses mir-17-92

Depositor

Lin He

Article

Olive et al Genes Dev. 2009 Dec 15. 23(24):2839-49.

Insert

miR-17-92 (MIR17HG Human)

Expression

Mammalian

Promoter

MSCV

Available Since

June 12, 2015

Availability

Academic Institutions and Nonprofits only

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MSCV-mir-92

Plasmid

#64092

Purpose

expresses miR-92

Depositor

Lin He

Article

Olive et al Genes Dev. 2009 Dec 15. 23(24):2839-49.

Insert

miR-92 (MIR92A1 Human)

Expression

Mammalian

Promoter

MSCV

Available Since

June 11, 2015

Availability

Academic Institutions and Nonprofits only

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