2,225 results

1. pAAV-CMV-DIO-TRPV4-p2A-ferritin-sNRPpA

   Plasmid

   #74306

   Purpose

   Bicistronic Cre-dependent expression of TRPV4 & ferritin in mammalian cells

   Depositor

   Ali Guler

   Article

   Wheeler et al Nat Neurosci. 2016 Mar 7. doi: 10.1038/n

   Inserts

   TRPV4 (Trpv4 Rat)
   ferritin (FTL and HTL1) (FTL, FTH1 Human)
   

   Use

   AAV and Cre/Lox

   Tags

   FLAG and p2A

   Expression

   Mammalian

   Mutation

   Promoter

   CMV (after cre recombination)

   Available since

   July 18, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV5-rat ERK2-K52R

   Plasmid

   #40813

   Depositor

   Natalie Ahn

   Article

   Emrick et al Proc Natl Acad Sci U S A. 2006 Nov 28;10

   Insert

   MAPK1 (Mapk1 Rat)
   

   Use

   Tags

   His6

   Expression

   Mammalian

   Mutation

   K52R

   Promoter

   CMV

   Available since

   Oct. 18, 2012

   Availability

   Academic Institutions and Nonprofits only

3. sq5

   Plasmid

   #24499

   Depositor

   Bruce Conklin

   Article

   Conklin et al Mol Pharmacol. 1996 Oct . 50(4):885-90.

   Insert

   Gs alpha (Gnas Rat)
   

   Use

   Tags

   HA (see comments)

   Expression

   Mammalian

   Mutation

   This is Gs alpha with the C-terminal amino acids …

   Promoter

   Available since

   Aug. 24, 2010

   Availability

   Academic Institutions and Nonprofits only

4. Myr.PKCzeta.FLAG

   Plasmid

   #10802

   Depositor

   Alex Toker

   Article

   Chou et al Curr Biol. 1998 Sep 24. 8(19):1069-77.

   Insert

   PKC zeta (Prkcz Rat)
   

   Use

   Tags

   FLAG and Myr

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 26, 2006

   Availability

   Academic Institutions and Nonprofits only

5. pMRX-IP-GFP-LC3-mRuby3

   Plasmid

   #184898

   Purpose

   Stable expression of GFP-LC3-mRuby3 in mammalian cells to measure autophagic flux

   Depositor

   Noboru Mizushima

   Article

   Okawa et al J Cell Sci. 2021 Apr 15;134(8). pii: 237

   Insert

   LC3B
   

   Use

   Tags

   EGFP and mRuby3

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEGFP-N1-VAPA(1-242) K87D/M89D

   Plasmid

   #18875

   Depositor

   Axel Brunger

   Article

   Kaiser et al Structure. 2005 Jul . 13(7):1035-45.

   Insert

   VAP-A (Vapa Rat)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   double point mutant K87D / M89D

   Promoter

   Available since

   Sept. 19, 2008

   Availability

   Academic Institutions and Nonprofits only

7. pAAV-U6-sgRNA(OXTR.1)-CMV-eGFP

   Plasmid

   #194015

   Purpose

   Expresses a gRNA that targets OXTR coding sequence in multiple (rodent) species and eGFP

   Depositor

   Larry Young

   Article

   Boender et al Sci Adv. 2023 Jun 2;9(22):eadf4950. doi:

   Inserts

   sgRNA(Oxtr.1)
   eGFP
   

   Use

   AAV and CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and u6

   Available since

   April 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pB-Neo-rTOM20-GFP

   Plasmid

   #210331

   Purpose

   Overexpression of rTOM20

   Depositor

   Li Yu

   Article

   Jiao et al Cell. 2021 May 27;184(11):2896-2910.e13.

   Insert

   rTOM20 (Tomm20 Rat)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pB-Hygro-rKIF5B-GFP

   Plasmid

   #210335

   Purpose

   Overexpression of rKIF5B

   Depositor

   Li Yu

   Article

   Jiao et al Cell. 2021 May 27;184(11):2896-2910.e13.

   Insert

   rKIF5B (Kif5b Rat)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pYFP-ErbB2ΔC990

    Plasmid

    #66946

    Purpose

    Mammalian expression of rat ErbB2 ΔC990 tagged with YFP

    Depositor

    Bo van_Deurs, Mads Lerdrup

    Article

    Lerdrup et al Mol Biol Cell. 2007 Sep;18(9):3656-66. E

    Insert

    ErbB2 (Erbb2 Rat)
    

    Use

    Tags

    6xHis, V5 Tag, and YFP

    Expression

    Mammalian

    Mutation

    Deletion of C990. (see depositor comment below on…

    Promoter

    CMV

    Available since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

11. p75 NTR

    Plasmid

    #24091

    Depositor

    Moses Chao

    Insert

    p75NTR (Ngfr Rat)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 2, 2013

    Availability

    Academic Institutions and Nonprofits only

12. pcDNA5-TO-PV-NLS-Dsred

    Plasmid

    #16341

    Depositor

    Anton Bennett

    Article

    Rodrigues et al J Biol Chem. 2007 Jun 8. 282(23):17061-8

    Insert

    PV-DsRed-NLS (Pvalb Rat)
    

    Use

    Tags

    Myc and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 12, 2008

    Availability

    Academic Institutions and Nonprofits only

13. pGEX-4T Syt1 C2AB(140) WT

    Plasmid

    #134695

    Purpose

    Encodes cytoplasmic domain of synaptotagmin-1 for bacterial expression and purification

    Depositor

    Edwin Chapman

    Article

    Bradberry et al J Biol Chem. 2019 Jul 12;294(28):10942-1

    Insert

    Synaptotagmin 1 (Syt1 Rat)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. Cav2.2e[a10,Δ18a, Δ24a, 31a, 37b, 46]

    Plasmid

    #26567

    Depositor

    Diane Lipscombe

    Article

    Lin et al Neuron. 1997 Jan . 18(1):153-66.

    Insert

    calcium channel alpha-1B subunit (Cacna1b Rat)
    

    Use

    Tags

    6XHis and V5

    Expression

    Mammalian

    Mutation

    Contains exons a10, 31a, 37b, and 46. Deletion…

    Promoter

    Available since

    Nov. 29, 2010

    Availability

    Academic Institutions and Nonprofits only

15. pcDNA3-EGFP-AC8(1-106)

    Plasmid

    #181958

    Purpose

    Residues 1-106 of rat adenylyl cyclase 8 (AC8) tagged with EGFP.

    Depositor

    Jin Zhang

    Article

    Tenner et al Elife. 2020 Nov 17;9. pii: 55013. doi: 1

    Insert

    EGFP-AC8(1-106) (Adcy8 Rat)
    

    Use

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pENTR1A-HA-Jag1

    Plasmid

    #46052

    Depositor

    Xin Chen

    Article

    Tschaharganeh et al Gastroenterology. 2013 Jun;144(7):1530-1

    Insert

    Jag1 (Jag1 Rat)
    

    Use

    Entry vector

    Tags

    HA tag

    Expression

    Mutation

    Promoter

    Available since

    July 5, 2013

    Availability

    Academic Institutions and Nonprofits only

17. mApple-Homer1-C-18

    Plasmid

    #54912

    Purpose

    Localization: Membrane Adaptor, Excitation: 568, Emission: 592

    Depositor

    Michael Davidson

    Insert

    Homer1 (Homer1 Rat)
    

    Use

    Tags

    mApple

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

18. pYFP-ErbB2ΔC776

    Plasmid

    #66947

    Purpose

    Mammalian expression of rat ErbB2 ΔC776 tagged with YFP

    Depositor

    Bo van_Deurs, Mads Lerdrup

    Article

    Lerdrup et al Mol Biol Cell. 2007 Sep;18(9):3656-66. E

    Insert

    ErbB2 (Erbb2 Rat)
    

    Use

    Tags

    6xHis, V5 Tag, and YFP

    Expression

    Mammalian

    Mutation

    Deletion of C776 (see depositor comment below on …

    Promoter

    CMV

    Available since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

19. pET14b PHAS-I

    Plasmid

    #15679

    Depositor

    John Lawrence

    Article

    Choi et al J Biol Chem. 2003 May 30. 278(22):19667-

    Insert

    PHAS-I (Eif4ebp1 Rat)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Nucleotides 1-354 of rat PHAS-I plus 450bp of 3&#…

    Promoter

    Available since

    Sept. 24, 2007

    Availability

    Academic Institutions and Nonprofits only

20. pWPXL-NP1

    Plasmid

    #72654

    Purpose

    Constitutive expression of NP1

    Depositor

    Ramon Trullas

    Article

    Figueiro-Silva et al J Neurosci. 2015 Apr 8;35(14):5504-21. d

    Insert

    NP1 (Nptx1 Rat)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. mStrawberry-Cx43-7

    Plasmid

    #55937

    Purpose

    Localization: Gap Junctions, Excitation: 574, Emission: 596

    Depositor

    Michael Davidson

    Insert

    Cx43
    

    Use

    Tags

    mStrawberry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. mStrawberry-Cx26-7

    Plasmid

    #55936

    Purpose

    Localization: Gap Junctions, Excitation: 574, Emission: 596

    Depositor

    Michael Davidson

    Insert

    Cx26
    

    Use

    Tags

    mStrawberry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pBactin-AcGFP-Cdk5rap2-CTD

    Plasmid

    #196853

    Purpose

    Expression of the centrosome targeting carboxy-terminal domain (CTD) of Cdk5rap2 fused to AcGFP. Used to displace endogenous Cdk5rap2 from centrosomes.

    Depositor

    Frank Bradke

    Article

    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

    Insert

    AcGFP-Cdk5rap2-CTD (Cdk5rap2 Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    None (wt)

    Promoter

    Chicken bactin (plus Chicken bactin intron)

    Available since

    May 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pSIN ReP5-GFP-GluR1

    Plasmid

    #24004

    Depositor

    Robert Malinow

    Article

    Kessels et al Nat Neurosci. 2009 Jul . 12(7):888-96.

    Insert

    GluR1 (Gria1 Rat)
    

    Use

    Sindbis virus production

    Tags

    GFP

    Expression

    Mutation

    Promoter

    Available since

    April 5, 2010

    Availability

    Academic Institutions and Nonprofits only

25. pRc/CMV HA TrkB-T1

    Plasmid

    #39980

    Depositor

    Yves-Alain Barde

    Article

    Bibel et al EMBO J. 1999 Feb 1;18(3):616-22.

    Insert

    TrkB.T1 (Ntrk2 Rat)
    

    Use

    Tags

    BM40 signal sequence and HA

    Expression

    Mammalian

    Mutation

    TrkB.T1 variant; endogenous signal sequence was r…

    Promoter

    CMV

    Available since

    March 17, 2014

    Availability

    Academic Institutions and Nonprofits only

26. Cav2.2e[Δa10, Δ18a, Δ24a, 31a, 37a, 46]

    Plasmid

    #26569

    Depositor

    Diane Lipscombe

    Article

    Bell et al Neuron. 2004 Jan 8. 41(1):127-38.

    Insert

    calcium channel alpha-1B subunit (Cacna1b Rat)
    

    Use

    Tags

    6XHis and V5

    Expression

    Mammalian

    Mutation

    Deletion of exons a10, 18a, and 24a. Contains …

    Promoter

    Available since

    Nov. 29, 2010

    Availability

    Academic Institutions and Nonprofits only

27. LUC2 (L/Sec/deltaP)

    Plasmid

    #112145

    Purpose

    Mammalian expression vector to test the effect of substituting selenocysteine for Cys on luciferase enzyme activity

    Depositor

    Paul Copeland

    Article

    Mehta et al J Biol Chem. 2004 Sep 3;279(36):37852-9.

    Insert

    Luciferase coding region with Cysteine 258 mutated to selenocysteine (U) and deleted AUGA in PHGPx SECIS core (Gpx4 Photinus pyralis, Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Cysteine 258 mutated to selenocysteine (U) and d…

    Promoter

    Available since

    July 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pcDNA3.1(+):RGS4

    Plasmid

    #31157

    Depositor

    Henry Lester

    Article

    Doupnik et al Proc Natl Acad Sci U S A. 1997 Sep 16. 9

    Insert

    Regulator of G Protein Signaling 4 (Rgs4 Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 10, 2011

    Availability

    Academic Institutions and Nonprofits only

29. pcDNA 3.1(-) rat C/EBP beta (LAP)

    Plasmid

    #12558

    Depositor

    Peter Johnson

    Insert

    CCAAT/Enhancer-binding Protein beta (Cebpb Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 18, 2007

    Availability

    Academic Institutions and Nonprofits only

30. pPA016 (delta 86-130 Parkin)

    Plasmid

    #102357

    Purpose

    expresses Rat Parkin with residues 86-130 deleted

    Depositor

    Kalle Gehring

    Article

    Sauve et al EMBO J. 2015 Oct 14;34(20):2492-505. doi

    Insert

    Parkin (Prkn Rat)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    deleted residues 86 to 130

    Promoter

    tac

    Available since

    Nov. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge