We narrowed to 56,851 results for: plasmid
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Plasmid#82033PurposePlasmid for constitutive or doxycycline-inducible expression of wild-type human MRE11. Confers resistance to puromycin. Use T-REx cells for doxycycline-inducible expression.DepositorAvailable SinceSept. 20, 2016AvailabilityAcademic Institutions and Nonprofits only
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pCMV-OptoSTIM1
Plasmid#70159PurposeMammalian expression plasmid of OptoSTIM1 (optogenetically-activated STIM1 protein)DepositorAvailable SinceNov. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRS416-dCas9-Mxi1 + TetR + pRPR1(TetO)-NotI-gRNA
Plasmid#73796PurposepRS416 Ura marked Cen/Ars plasmid with dCas9-Mxi1 under Tef1 promoter, and tet-incucibile RPR1 promoter with NotI cloning site adjacent to gRNADepositorInsertsdCas9-Mxi1
Tet Repressor
Structural gRNA for S pyogenes
UseCRISPRTagsMxi1 and NLSExpressionYeastMutationD10A, H840APromoterpGPM1, pRPR1(TetO), and pTef1Available SinceMarch 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
PG13-luc (wt p53 binding sites)
Plasmid#16442PurposeLuciferase reporter containing 13 copies of the p53-binding consensus sequenceDepositorAvailable SinceMarch 12, 2008AvailabilityAcademic Institutions and Nonprofits only -
PB-TRE-EGFP-EF1a-rtTA
Plasmid#104454PurposePiggyBac plasmid expressing EGFP under the control of a doxycycline-inducible promotorDepositorInsertsenhanced green fluorescent protein
reverse tetracycline-controlled transactivator 3
UsePiggybacExpressionMammalianAvailable SinceApril 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMLM3705
Plasmid#47754PurposeExpresses mammalian cell codon-optimized dCas9-VP64DepositorInsertcodon optimized dCas9-VP64
UseCRISPRTags3x FLAG, NLS, and VP64ExpressionMammalianMutationD10A and H840A mutations in Cas9 (catalytically i…PromoterCMVAvailable SinceAug. 27, 2013AvailabilityAcademic Institutions and Nonprofits only -
pENN.AAV.CamKII.HI.GFP-Cre.WPRE.SV40
Plasmid#105551PurposeAAV expression of GFP-Cre from CamKII promoterDepositorHas ServiceAAV Retrograde and AAV9InsertEGFP-Cre
UseAAV and Cre/LoxExpressionMammalianPromoterCamKIIAvailable SinceMay 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-ABEmax(7.10)-SpG-P2A-EGFP (RTW4562)
Plasmid#140002PurposeCMV and T7 promoter expression plasmid for human codon optimized ABEmax(7.10) A-to-G base editor with SpG(D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R) and P2A-EGFPDepositorInserthuman codon optimized ABEmax(7.10) SpCas9 variant named SpG with P2A-EGFP
UseCRISPR; In vitro transcription; t7 promoterTagsP2A-EGFPExpressionMammalianMutationnSpCas9=D10A; SpG=D1135L/S1136W/G1218K/E1219Q/R13…PromoterCMV and T7Available SinceMarch 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLentiPGK Puro DEST p38KTRClover
Plasmid#59152PurposeLentiviral vector to express p38 KTR mClover under PGK promoter (With Puromycin Resistance)DepositorInsertp38 Kinase Translocation Reporter (MAPK14 Human, Mouse)
UseLentiviralExpressionMammalianPromoterPGKAvailable SinceSept. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDTA-TK
Plasmid#22677DepositorInsertDTA and TK
UseMouse TargetingMutationpDTA-TK dual selection plasmid for targeting vect…Available SinceDec. 4, 2009AvailabilityAcademic Institutions and Nonprofits only -
pET-28b-RfxCas13d-His
Plasmid#141322PurposePlasmid for bacterial expression and purification of RfxCas13d proteinDepositorInsertRfx-Cas13d
UseCRISPRTags6xHisExpressionBacterialPromoterT7Available SinceAug. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLenti-FoxO1-Clover
Plasmid#67759PurposeFluorescent fusion protein for tracking Akt signalingDepositorInsertFox01 (Foxo1 Mouse)
UseLentiviralTagsCloverMutationChanged Serine 209 to Alanine, changed Histidine …PromoterEF1aAvailable SinceAug. 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
GFP-C1-AKT-PH
Plasmid#21218DepositorInsertAKT-PH
TagsGFPExpressionMammalianMutationPH domain of AKT. (Lab plasmid WC 0002).Available SinceJuly 10, 2009AvailabilityAcademic Institutions and Nonprofits only -
pSico p53
Plasmid#12089PurposeCre-regulated lentiviral shRNA vector. Cre addition causes EGFP to be recombined out of the construct, activating p53 shRNA expression.DepositorInsertp53 shRNA (Trp53 Mouse)
UseCre/Lox, Lentiviral, and RNAiExpressionMammalianMutationEGFP is expressed from this plasmid as a marker, …Available SinceAug. 4, 2006AvailabilityAcademic Institutions and Nonprofits only -
NFAT luciferase reporter
Plasmid#10959DepositorAvailable SinceJan. 6, 2006AvailabilityAcademic Institutions and Nonprofits only -
AAV.rTH.PI.Cre.SV40
Plasmid#107788PurposeAAV vector expressing Cre from rTH promoterDepositorHas ServiceAAV Retrograde and AAV9InsertCre
UseAAVExpressionMammalianPromoterrTHAvailable SinceMay 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
LentiGuide-Neo
Plasmid#139449PurposeLentiviral vector with gRNA scaffold and neomycin selectable markerDepositorInsertno sgRNA inserted; resistance gene: neoR
UseLentiviralExpressionMammalianPromoterEF-1a / U6Available SinceAug. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLentiPGK Blast DEST JNKKTRmRuby2
Plasmid#59154PurposeLentiviral vector to express JNK KTR mRuby2 under PGK promoter (With Blasticidin Resistance)DepositorInsertJNK Kinase Translocation Reporter (MAPK8 Human)
UseLentiviralTagsmRuby2ExpressionMammalianPromoterPGKAvailable SinceSept. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCGN-HCF-1 fl
Plasmid#53309Purposeplasmid contains human HCFC1 full length gene with HA and c-myc tagsDepositorAvailable SinceMay 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
pYPQ202
Plasmid#86198PurposeMultisite Gateway T-DNA entry plasmid (attR1 & attR2); AtUBQ10 promoter and Hygromycin resistance markerDepositorTypeEmpty backboneUseGateway compatible attr1-attr2 destination vectorExpressionPlantPromoterArabidopsis ubiquitin 10 (AtUbi10)Available SinceMay 2, 2017AvailabilityAcademic Institutions and Nonprofits only