We narrowed to 26,139 results for: Vit

1. His-TEV-Aurein1.2_(+36GFP)-LPETG-His

   Plasmid

   #71755

   Purpose

   His-TEV-Aurein1.2+36GFP-LPETG-His expression in E.Coli

   Depositor

   David Liu

   Article

   Li et al J Am Chem Soc. 2015 Nov 11;137(44):14084

   Insert

   Aurein1.2_(pos36_GFP)-LPETG-His
   

   Use

   Tags

   6XHis, Sortase (LPETG), and TEV Protease

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-E1.1-RFP

   Plasmid

   #22928

   Depositor

   Edward Callaway

   Article

   Nathanson et al Front Neural Circuits. 2009 Nov 9;3:19.

   Insert

   E1.1 binding site from Scardigli et al., 2003 with minimal CMV
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 25, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTagBFP-Akt1

   Plasmid

   #86594

   Purpose

   Expresses TagBFP-tagged human Akt1(WT)

   Depositor

   Ivan Yudushkin, Thomas Leonard

   Article

   Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

   Insert

   Akt1 (AKT1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pUB-Cas9

   Plasmid

   #86556

   Purpose

   For ubiquitous expression of Cas9 in planta. sgRNA can be introduced as described in Hahn et al. (2017)

   Depositor

   Andreas Weber

   Article

   Hahn et al Front Plant Sci. 2017 Jan 24;8:39. doi:

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Cas9 is codon optimized for C. reinhardtii

   Promoter

   UBIQUITIN10

   Available since

   May 16, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV4a-SIRT6-H133Y-FLAG

   Plasmid

   #102324

   Purpose

   Human SIRT6 H133Y with FLAG tag for mammalian expression

   Depositor

   Hening Lin

   Article

   Zhang et al Nat Chem Biol. 2016 Aug;12(8):614-20. do

   Insert

   SIRT6 (SIRT6 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   changed histidine 133 to tyrosine

   Promoter

   CMV

   Available since

   Oct. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pILGFP92

   Plasmid

   #83549

   Purpose

   Used to evaluate the expression output of TDH3 promoter with yEGFP used as the reporter gene.

   Depositor

   Claudia Vickers

   Article

   Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

   Insert

   TDH3 promoter-yEGFP
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   TDH3

   Available since

   Oct. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pVETL-Cmcs-Flag-Ataxin3Q25

   Plasmid

   #22118

   Depositor

   Henry Paulson

   Article

   Todi et al J Biol Chem. 2007 Oct 5. 282(40):29348-5

   Insert

   Ataxin 3 (ATXN3 Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 21, 2009

   Availability

   Academic Institutions and Nonprofits only

8. pCyPet-Rac1(WT)

   Plasmid

   #22782

   Depositor

   Klaus Hahn

   Article

   Machacek et al Nature. 2009 Sep 3. 461(7260):99-103.

   Insert

   Rac1 (wt) (RAC1 Human)
   

   Use

   Tags

   CyPet

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 22, 2010

   Availability

   Academic Institutions and Nonprofits only

9. pcDNA3.1-mas-KB-1753-Nluc

   Plasmid

   #158603

   Purpose

   Plasmid expressing KB-1753-Nluc in mammalian cells

   Depositor

   Mikel Garcia-Marcos

   Article

   Maziarz et al Cell. 2020 Jun 27. pii: S0092-8674(20)30

   Insert

   myristic acid attachment peptide (mas; MGSSKSKTSNS) - linker(GIKLGG) - KB1753 - linker (GGTGGS) - Nluc
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 30, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. CANE-LV envelope

    Plasmid

    #86666

    Purpose

    pCAGGS/ES-M21EnvA-VSVg-WPRE, used to package pseudotyped lentivirus

    Depositor

    Fan Wang

    Article

    Sakurai et al Neuron. 2016 Nov 23;92(4):739-753. doi:

    Insert

    CANE-LV envelope
    

    Use

    Lentiviral, Mouse Targeting, and Synthetic Biolog…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Feb. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

11. pcDNA3-DAKAP-μDpTT

    Plasmid

    #182324

    Purpose

    Mitochondria-targeted Dronpa chromophore-removed FLINC (DrFLINC) pair. Fluctuating red-fluorescent label for live-cell super-resolution imaging.

    Depositor

    Jin Zhang

    Article

    Lin et al J Am Chem Soc. 2021 Sep 22;143(37):14951

    Insert

    DAKAP-μDronpa-TagRFP-T
    

    Use

    Tags

    N-terminal 30 amino acids from DAKAP1

    Expression

    Mammalian

    Mutation

    Dronpa contains H193T mutation.

    Promoter

    CMV

    Available since

    May 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-hSynapsin1-axon-GCaMP6f

    Plasmid

    #112000

    Purpose

    For enriched expression of GCaMP6f in axons

    Depositor

    Lin Tian

    Article

    Broussard et al Nat Neurosci. 2018 Sep;21(9):1272-1280.

    Has Service

    AAV1

    Insert

    axon-GCaMP6f
    

    Use

    AAV

    Tags

    GAP43 palmitoylation domain

    Expression

    Mammalian

    Mutation

    Promoter

    hSynapsin1

    Available since

    June 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDONR221_SLC22A1

    Plasmid

    #132034

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    Giulio Superti-Furga, RESOLUTE Consortium

    Article

    Mocking et al Int J Mol Sci. 2022 Jan 21;23(3):1203. d

    Insert

    SLC22A1 (SLC22A1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 25, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pcDNA3-Nuc-ExRai-AMPKAR

    Plasmid

    #192450

    Purpose

    ExRai-AMPKAR targeted to the nucleus.

    Depositor

    Jin Zhang

    Article

    Schmitt et al Nat Commun. 2022 Jul 5;13(1):3856. doi:

    Insert

    ExRai-AMPKAR-NLS
    

    Use

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3-Mito-CaNAR2

    Plasmid

    #64731

    Purpose

    Mitochondria-targeted FRET biosensor for monitoring calcineurin (CaN) phosphatase activity; targeted to cytosolic face of mitochondrial outer membrane.

    Depositor

    Jin Zhang

    Article

    Mehta et al eLife. 2014 Jul 23;3:e03765. doi: 10.755

    Insert

    CaNAR2
    

    Use

    Tags

    Cerulean3, N-terminal 30 amino acids from DAKAP1,…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET28a-His-MinD_MinE

    Plasmid

    #133621

    Purpose

    Expression vector for the Escherichia coli MinD with an N-terminal His-tag for purification. MinE is encoded in the ORF to avoid toxicity when overexpressing MinD, but MinE is not tagged.

    Depositor

    Petra Schwille

    Article

    Loose et al Science. 2008 May 9;320(5877):789-92. do

    Insert

    His-MinD_MinE (minD, minE E. coli)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. p1071-Lenti-Single loxP

    Plasmid

    #217892

    Purpose

    Lentiviral vector, entry vector for Lentiviral Switch-OVER

    Depositor

    Ulrich Elling

    Article

    Chylinski et al Nat Commun. 2019 Nov 29;10(1):5454. doi:

    Type

    Empty backbone

    Use

    CRISPR, Cre/Lox, and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    May 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. N1-mSin1.2-GFP

    Plasmid

    #72908

    Purpose

    GFP-tagged mSin1 (isoform 2)

    Depositor

    Ivan Yudushkin

    Article

    Ebner et al J Cell Biol. 2017 Jan 31. pii: jcb.20161

    Insert

    Target of rapamycin complex 2 subunit MAPKAP1, isoform 2 (MAPKAP1 Human)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET21b-Caspase-3(D3A,V266E)

    Plasmid

    #90091

    Purpose

    Expresses caspase D3A and V266E mutant in bacterial cells

    Depositor

    Clay Clark

    Article

    Pop et al Biochemistry. 2003 Oct 28;42(42):12311-2

    Insert

    Caspase 3 (CASP3 Human)
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    D175A D28A D9A V266E

    Promoter

    Available since

    May 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pRSV-CaMKII

    Plasmid

    #45064

    Purpose

    Expression of CaMKII

    Depositor

    Richard Maurer

    Article

    Sun et al Genes Dev. 1994 Nov 1;8(21):2527-39.

    Insert

    CaM Kinase II (Camk2a Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    RSV

    Available since

    July 30, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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