4,143 results

1. GFP-EEA1 wt

   Plasmid

   #42307

   Depositor

   Silvia Corvera

   Article

   Lawe et al J Biol Chem. 2000 Feb 4;275(5):3699-705.

   Insert

   EEA1 (EEA1 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   none

   Promoter

   CMV

   Available since

   Jan. 31, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLenti6.3/V5-DEST-GFP

   Plasmid

   #40125

   Depositor

   Lynda Chin

   Article

   Quayle et al Neuro Oncol. 2012 Nov;14(11):1325-31. do

   Insert

   GFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 16, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. SP-Cas9

   Plasmid

   #62731

   Purpose

   Expresses SP-Cas9 protein in bacterial cells

   Depositor

   Niels Geijsen

   Article

   D'Astolfo et al Cell. 2015 Apr 23;161(3):674-690. doi: 1

   Insert

   SP-CAS9
   

   Use

   CRISPR

   Tags

   His tag and NLS

   Expression

   Bacterial

   Mutation

   Codon optimazed for e.coli

   Promoter

   T7

   Available since

   April 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEVOL-pAzF

   Plasmid

   #31186

   Purpose

   tRNA synthetase/tRNA pair for the in vivo incorporation of a photocrosslinker, p-azido-l-phenylalanine, into proteins in E coli response to the amber codon, TAG

   Depositor

   Peter Schultz

   Article

   Chin et al J Am Chem Soc. 2002 Aug 7;124(31):9026-7

   Insert

   M.j. p-azidohenylalanine RS (2 copies +tRNA)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Y32T, E107N, D158P, I159L, L162Q, D286R

   Promoter

   Available since

   Dec. 9, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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5. psfaRb-Tn5

   Plasmid

   #183638

   Purpose

   Expresses anti-rabbit nanobody fused with Tn5

   Depositor

   Goncalo Castelo-Branco

   Article

   Bartosovic et al Nat Biotechnol. 2023 Jun;41(6):794-805.

   Insert

   anti rabbit secondary nanobody fused to Tn5
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. PB_tre_Cas9

   Plasmid

   #126029

   Purpose

   PiggyBac cargo vector expressing doxycycline inducible Cas9

   Depositor

   Mauro Calabrese

   Article

   Schertzer et al RNA. 2019 May 17. pii: rna.068932.118. d

   Inserts

   humanized S. pyrogenes Cas9
   Hygromycin resistance
   

   Use

   CRISPR; Piggybac

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1 alpha and TRE

   Available since

   June 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEGFP-puro

   Plasmid

   #45561

   Depositor

   Michael McVoy

   Article

   Abbate et al Biotechniques. 2001 Aug;31(2):336-40.

   Insert

   Puromycin N-acetyl transferase
   

   Use

   Bifunctional selection protein

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Phcmv (Strong human cytomegalovirus immediate ear…

   Available since

   June 13, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. pYL192 (TRV1)

   Plasmid

   #148968

   Purpose

   Tobacco Rattle Virus RNA 1; used for virus induced gene silencing in plants along with pYL156 (TRV RNA2)

   Depositor

   Savithramma Dinesh-Kumar

   Article

   Liu et al Plant J. 2002 May;30(4):415-29. doi: 10.

   Insert

   TRV RNA1
   

   Use

   Plant vector with trv rna1; this vector used with…

   Tags

   Expression

   Mutation

   P697S mutation in putative replicase. S239G and S…

   Promoter

   Available since

   June 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. Tet-pLKO-neo

   Plasmid

   #21916

   Purpose

   3rd generation lentiviral plasmid for inducible expression of shRNA; neomycin selection. See manual for detailed protocols. No further technical support is available from the depositing laboratory.

   Depositor

   Dmitri Wiederschain

   Article

   Wiederschain et al Cell Cycle. 2009 Feb 1. 8(3):498-504.

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 22, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSF3-ultraID

    Plasmid

    #172878

    Purpose

    expresses ultraID in mammalian cells with the tet-on or tet-off system

    Depositor

    Julien Béthune

    Article

    Kubitz et al Commun Biol. 2022 Jul 4;5(1):657. doi: 1

    Insert

    ultraID
    

    Use

    Luciferase and Retroviral; To be used in a tet-on…

    Tags

    myc

    Expression

    Mammalian

    Mutation

    fragment ([aa2-171]) of aquifex aeolicus BirA wit…

    Promoter

    TRE-cmv

    Available since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMSP1E3

    Plasmid

    #20064

    Depositor

    Stephen Sligar

    Article

    Denisov et al J Am Chem Soc. 2004 Mar 24. 126(11):3477

    Insert

    MSP1E3 (APOA1 synthetic gene)
    

    Use

    Tags

    6-His

    Expression

    Bacterial

    Mutation

    "extended" MSP1; contains repeats of he…

    Promoter

    Available since

    March 16, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA-Ikkb-FLAG WT

    Plasmid

    #23298

    Depositor

    Warner Greene

    Article

    Geleziunas et al Mol Cell Biol. 1998 Sep . 18(9):5157-65.

    Insert

    Ikkbeta (IKBKB Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 24, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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13. TetO-Ascl1-puro

    Plasmid

    #97329

    Purpose

    Generating lentiviruses to express Ascl1 and puromycin resistance

    Depositor

    Marius Wernig

    Article

    Yang et al Nat Methods. 2017 May 15. doi: 10.1038/n

    Insert

    Ascl1 (Ascl1 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    TET-ON

    Available since

    July 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMXs-c-Myc

    Plasmid

    #13375

    Purpose

    Retroviral expression of mouse c-Myc

    Depositor

    Shinya Yamanaka

    Article

    Takahashi et al Cell. 2006 Aug 25. 126(4):663-76.

    Insert

    myelocytomatosis oncogene (Myc Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    None

    Promoter

    Available since

    Jan. 12, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHR_Gal4UAS_IRES_mC_pGK_tBFP

    Plasmid

    #79123

    Purpose

    Lentiviral vector for inducible expression of genes with Gal4DBDVP64 synNotch Receptors

    Depositor

    Wendell Lim

    Article

    Morsut et al Cell. 2016 Feb 11;164(4):780-91. doi: 10

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.1_AGIA-AirID

    Plasmid

    #182210

    Purpose

    Express AirID in mammalian cells.

    Depositor

    Tatsuya Sawasaki, Sohei Ito, Akira Nozawa, Shogo Nakano

    Article

    Kido et al Elife. 2020 May 11;9. pii: 54983. doi: 1

    Insert

    AGIA-AirID
    

    Use

    Tags

    AGIA-tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pQE 80L Kan T7WT

    Plasmid

    #102790

    Purpose

    Expresses T7 RNA polymerase

    Depositor

    Andrew Ellington

    Article

    Abil et al Nat Protoc. 2017 Dec;12(12):2493-2512. d

    Insert

    T7 RNA polymerase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 3, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. pLenti6/V5-p53_R273H

    Plasmid

    #22934

    Depositor

    Bernard Futscher

    Article

    Junk et al Neoplasia. 2008 May . 10(5):450-61.

    Insert

    tumor protein 53 (TP53 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    R273H Mutant

    Promoter

    Available since

    Jan. 27, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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19. lentiCRISPRv2 blast

    Plasmid

    #98293

    Purpose

    Variant of lentiCRISPRv2 that confers blasticidin S resistance

    Depositor

    Brett Stringer

    Article

    Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF-1a

    Available since

    July 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pML107

    Plasmid

    #67639

    Purpose

    Expresses Cas9 and contains guide RNA expression cassette with BclI-SwaI cloning sites for guide sequence cloning; Contains LEU2 marker for yeast transformation

    Depositor

    John Wyrick

    Article

    Laughery et al Yeast. 2015 Dec;32(12):711-20. doi: 10.1

    Inserts

    Cas9
    single guide RNA expression cassette
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    pSNR52 and pTDH3 (aka GAP promoter)

    Available since

    Aug. 31, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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