We narrowed to 18,759 results for: nls
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Plasmid#176268PurposeExpression of dCas12f-VPR-mCherry in mammalian cellsDepositorInsertdCas12f
UseLentiviralTagsHA-VPR-mCherryExpressionMammalianMutationD326A, D510APromoterPGKAvailable sinceOct. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
ePPEplus
Plasmid#205241PurposeFor plant prime editing in wheat plants or monocotyledons protoplastsDepositorInsertc-myc NLS-bpNLS-nCas9-NC-MLV-vbpNLS-SV40NLS
UseCRISPRExpressionPlantMutationDetailed in manuscriptAvailable sinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDY0738 pCAG-NLS-SscINT
Plasmid#179113PurposeSscINT expression vectorDepositorInsertpCAG-NLS-SscINT
UseCRISPRAvailable sinceFeb. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDY0739 pCAG-NLS-SacINT
Plasmid#179114PurposeSacINT expression vectorDepositorInsertpCAG-NLS-SacINT
UseCRISPRAvailable sinceFeb. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT2-7xTcf-NLS-CNL
Plasmid#65712PurposeTol2-based Wnt signal reporter plasmid (expresses NLS-CNL)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsert7xTcf, minCMV, 3xNLS, CNL
UseLuciferaseAvailable sinceFeb. 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGLTR-X-GFP
Plasmid#58245PurposeGATEWAY-compatible lentiviral conditional RNAi delivery vector; SFFV-driven expression of TetR and eGFPDepositorInsertsattR1-Cm(R)-CcdB-attR2
TetR-NLS-T2A-GFP
UseLentiviral and RNAi; Gateway destionation vectorTagsGFP, NLS, T2A, and TetRExpressionMammalianPromoterSFFV and noAvailable sinceNov. 7, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pNB648
Plasmid#60140PurposeNanoLuc luciferase with a nuclear localization signal (NLS) driven by MET17 promoter.DepositorInsertNLuc
TagsNuclear localization signal (NLS)ExpressionYeastPromoterMET17prAvailable sinceMarch 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-IRES-puro-NLS-dPguCas13b-3xEGFP-NLS-3xFlag
Plasmid#132399Purposeoverexpression in human cellsDepositorInsertdPguCas13b
UseLentiviralTagsflagExpressionMammalianMutationH151A; H1121AAvailable sinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
IscB-v3
Plasmid#227015PurposeVector encoding human codon-optimized enhanced-activity OgeuIscB driven by CMV promoterDepositorInsertSV40 NLS-OgeuIscB-v3-nucleoplasmin NLS
MutationD96R/E84R/V159RPromoterCMVAvailable sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
NK613
Plasmid#109091PurposeReporter gene expression in S. rosettaDepositorInsertmCherry_SV40 NLS
UseChoanoflagellate expressionAvailable sinceJune 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
NLS-Halo-HSF2
Plasmid#200198PurposeExpression of full length HSF2 with SV40 NLSDepositorInsert3X SV40 NLS-Halo-HSF2 (Hsf2 Mouse)
TagsHaloExpressionMammalianMutationNonePromoterEF_1alphaAvailable sinceMay 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGGC012
Plasmid#48826PurposeProvides GFP:NLS as GreenGate coding sequence module.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertGFP:NLS
UseGolden gate compatible cloning vectorAvailable sinceJan. 7, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSin_EF1a_tagBFP_P2A_NLS_pMag_CreC60_IRES_PuroR
Plasmid#176311PurposeExpresses NLS-pMag-CreC60 with tagBFP markerDepositorInserttagBFP_P2A_NLS_pMag_CreC60_IRES_PuroR
UseLentiviral and Synthetic BiologyTagstagBFPExpressionMammalianPromoterhuman EF1alphaAvailable sinceFeb. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
CuO-LacI-NLS–SNAPf
Plasmid#227368PurposeLentiviral expression of LacI–SNAPDepositorInsertLacI NLS SNAPf
UseLentiviralExpressionMammalianAvailable sinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-N-Flag-ASC1
Plasmid#75134PurposeExpresses N-terminal Flag-tagged ASC1 in mammalian cellsDepositorInsertPYD and CARD domain containing (Pycard Mouse)
TagsFLAGExpressionMammalianMutationF23S (please see depositor comments below)PromoterCMVAvailable sinceAug. 31, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pT2-7xTcf-NLS-ONL
Plasmid#65713PurposeTol2-based Wnt signal reporter plasmid (expresses NLS-ONL)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsert7xTcf, minCMV, 3xNLS, ONL
UseLuciferaseAvailable sinceJan. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-N-Flag-Caspase-1
Plasmid#75128PurposeExpresses N-terminal Flag-tagged Caspase-1 in mammalian cellsDepositorInsertcaspase 1 (Casp1 Mouse)
TagsFlagExpressionMammalianMutationP341S in Caspase-1 (please see depositor comments…PromoterCMVAvailable sinceMay 19, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
NLS-SpCas9mSA-His
Plasmid#182951PurposeExpress SpCas9mSA in bacteriaDepositorInsertNLS-SpCas9mSA-His
UseCRISPRExpressionBacterialAvailable sinceApril 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pQC NLS mKorange IX
Plasmid#37346DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertNLS mKusabira Orange
Available sinceJuly 27, 2012AvailabilityAcademic Institutions and Nonprofits onlyCited by -
phage - ubc - nls - 2xmcp - egfp - BirA*
Plasmid#131132PurposeBirA* expressed as a protein fusion with MS2 coat protein (MCP) dimer and EGFP; to visualize any MS2 tagged mRNAs and to identify the RNA proximal proteome by their biotinylation.DepositorInsertNLS-HA-2xMCP-EGFP-BirA*
UseLentiviralTagsNLS, HAExpressionMammalianMutationBirA* = BIrA >R118GPromoterhuman ubiquitin C (UBC)Available sinceAug. 30, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
AAV2-ITR-pEFS-NLS_hfCas13d(RfxCas13d_N2V8)_FLAG_NLS-pA-U6-DR-Pcsk9-sg5-DR-Pcsk9-sg6-DR-AAV2-ITR
Plasmid#191795Purposevector for encoding and AAV packaging a human codon-optimized High-fidelity RfxCas13d (hfCas13d) driven by EFS promoter, Pcsk9-target guide RNAs compatible with RfxCas13d driven by hU6DepositorInserthumanized hfCas13d
UseAAV and CRISPRExpressionMammalianMutationA134V, A140V, A141V, A143VPromoterEFS, hU6Available sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCMV-pro-Il1b-C-Flag
Plasmid#75131PurposeExpresses C-terminal flag-tagged pro-IL-1β in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertinterleukin 1 beta (Il1b Mouse)
Tags3XFLAGExpressionMammalianMutation(please see depositor comments below)PromoterCMVAvailable sinceSept. 13, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pU6-SasgRNA scaffold-CMV-nSaCas9
Plasmid#171695PurposeExpresses nSaCas9 in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsgRNA scaffold-nSaCas9-NLS
UseCRISPRExpressionMammalianMutationD10APromoterU6 and CMVAvailable sinceAug. 9, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
PX458-3xHA-SpCas9
Plasmid#130968Purpose3xHA tagged Cas9 from S. pyogenes with T2A-EGFP and cloning backbone for sgRNADepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsert3xHA-NLS-SpCas9
UseCRISPRTags3x HA, EGFP, and NLSExpressionMammalianPromoterCbhAvailable sinceNov. 7, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
p35S-PBE
Plasmid#119772PurposeConversion of C to T in potatoDepositorInsertNLS-APOBEC1-nCas9-UGI-NLS
TagsNLSExpressionPlantMutationnCas9 has a mutation D10A in wild type SpCas9PromoterCaMv 2x35SAvailable sinceSept. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
EmptyTranscriptionalReporter
Plasmid#62453PurposeMXS_chaining vector with 2 cassettesDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsCMVmin::NLS-Citrine-bGHpA
CMV::NLS-Cherry-bGHpA
UseSynthetic BiologyAvailable sinceMay 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-N-HA-NEK7
Plasmid#75142PurposeExpresses N-terminal HA-tagged NEK7 in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertNIMA (never in mitosis gene a)-related expressed kinase 7 (Nek7 Mouse)
TagsHAExpressionMammalianPromoterCMVAvailable sinceMay 20, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pEN_TT mCh-V5-NES-EGFP-SV40 NLS
Plasmid#192318PurposeGateway entry vector for an inducible mCh-V5-NES-EGFP-SV40 NLSDepositorInsertmCh-V5-NES-EGFP-SV40 NLS
UseGateway CloningTags3xFLAGAvailable sinceApril 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-NES-DEVD-mCardinal-NLS
Plasmid#164052PurposeFluorescent reporter for caspase-3DepositorPublicationHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertNES-mCardinal-NLS
Tagskozak sequenceExpressionMammalianPromoterCMV promotorAvailable sinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
mini-donor - NKX2.5-nEGFP
Plasmid#209287Purposethis mini-donor knockin vector can be used to knock nuclear EGFP into NKX2.5 locusDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertEGFP-nls
UseKnockin donor vectorAvailable sinceApril 5, 2024AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pHR_TRE-->2xNLS_IFP_PGK_PuroR
Plasmid#162238PurposeLentiviral vector for the expression of TRE-->2xNLS_IFP_PGK_PuroR in mammalian cellsDepositorInsertNLS IFP
UseLentiviralExpressionMammalianAvailable sinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBFv-nosP-Cas9
Plasmid#138402PurposeExpresses Cas9 in the germline of DrosophilaDepositorInsertCas9-NLS
ExpressionInsectPromoterUASAvailable sinceMarch 19, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA5/FRT_HaloTag7-CLIP-tag2-NLS-P2A-NLS-mTurquoise2
Plasmid#254206PurposeMamalian cell expression of nuclear HaloTag7-CLIP-tag2 and nuclear mTurquoise2DepositorPublicationInsertHaloTag7-CLIP-tag2-NLS-P2A-NLS-mTurquoise2
ExpressionMammalianPromoterCMVAvailable sinceApril 8, 2026AvailabilityAcademic Institutions and Nonprofits only -
CAG-mKate2-NLS
Plasmid#248044PurposeEncodes nuclear mKate2 fluorescent protein, under control of the CAG promoter. The construct is flanked by Tol2 sites for transgenesis.DepositorInsertmKate2-NLS
ExpressionBacterialMutationmKate2 was derived from mKate with the following …PromoterCAGAvailable sinceMay 29, 2026AvailabilityAcademic Institutions and Nonprofits only -
ACE16-NLS
Plasmid#210727PurposeNIR light-activated intein-based gene expression system (C-part)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertACE16-NLS
ExpressionMammalianPromoterCMVAvailable sinceDec. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
NLS-CanlonicSF
Plasmid#188390PurposeTo explore lactate dynamics in intact systemsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertNLS-CanlonicSF
TagsNLS and mycExpressionMammalianPromoterCMVAvailable sinceSept. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMEH295 NLS dTomato 1x wild type MS2 loop
Plasmid#168214PurposeMammalian expression of nuclear-localized dTomato with 1 wild type MS2 loopDepositorInsertNLS dTomato
UseLentiviralTagsNLSExpressionMammalianPromoterCMVAvailable sinceDec. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1 RPEL1-EGFP-3XNLS
Plasmid#58469PurposeExpresses a nuclear-targeted monomeric actin reporter, consisting of the RPEL1 domain from MAL/MRTF, on a CMV promoterDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsTagsEGFPExpressionMammalianPromoterCMVAvailable sinceApril 24, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Frb-NLS-mCherry
Plasmid#217613Purposepart B of SPARK-OFFDepositorInsertFrb-NLS-mCherry
UseLentiviralExpressionMammalianAvailable sinceJune 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
SBE-NLS-mCherry PGK-rtTA3
Plasmid#195332PurposeLentiviral construct of TGFβ reporter (nuclear mCherry) and hPGK driven rtTA3DepositorInsertsNLS-mCherry
rtTA3
UseLentiviral and RNAiExpressionMammalianPromoterhPGK and miniCMVAvailable sinceFeb. 13, 2023AvailabilityAcademic Institutions and Nonprofits only