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We narrowed to 1,793 results for: plasmid for e coli

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Showing: 1361 - 1400 of 1793 results

  1. M225I USP7 catalytic

    Plasmid
    #227206
    Purpose
    Expresses human USP7 Catalytic domain with a N-terminal cleavable 6His tag in E. coli
    Depositor
    Irina Bezsonova
    Insert
    USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
    Tags
    6His
    Expression
    Bacterial
    Mutation
    M225I; aa 207-560
    Promoter
    T7-promoter
    Available Since
    Oct. 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    227206-plasmid-map-sequence-id-449545

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  2. USP7 N418S catalytic

    Plasmid
    #227202
    Purpose
    Expresses human USP7 Catalytic domain with a N-terminal cleavable 6His tag in E. coli
    Depositor
    Irina Bezsonova
    Insert
    USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
    Tags
    6His
    Expression
    Bacterial
    Mutation
    N418Sl; aa 207-560
    Promoter
    T7-promoter
    Available Since
    Oct. 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    227202-plasmid-map-sequence-id-449544

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  3. L373F FL USP7

    Plasmid
    #227220
    Purpose
    Expresses human USP7 with a N-terminal cleavable 6His tag in E. coli
    Depositor
    Irina Bezsonova
    Insert
    USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
    Tags
    6His
    Expression
    Bacterial
    Mutation
    L373F
    Promoter
    T7-promoter
    Available Since
    Oct. 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    227220-plasmid-map-sequence-id-449541

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  4. Y331H FL USP7

    Plasmid
    #227218
    Purpose
    Expresses human USP7 with a N-terminal cleavable 6His tag in E. coli
    Depositor
    Irina Bezsonova
    Insert
    USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
    Tags
    6His
    Expression
    Bacterial
    Mutation
    Y331H
    Promoter
    T7-promoter
    Available Since
    Oct. 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    227218-plasmid-map-sequence-id-449540

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  5. P273H FL USP7

    Plasmid
    #227221
    Purpose
    Expresses human USP7 with a N-terminal cleavable 6His tag in E. coli
    Depositor
    Irina Bezsonova
    Insert
    USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
    Tags
    6His
    Expression
    Bacterial
    Mutation
    P273H
    Promoter
    T7-promoter
    Available Since
    Oct. 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    227221-plasmid-map-sequence-id-449538

    Enlarge

  6. M225I FL USP7

    Plasmid
    #227219
    Purpose
    Expresses human USP7 with a N-terminal cleavable 6His tag in E. coli
    Depositor
    Irina Bezsonova
    Insert
    USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
    Tags
    6His
    Expression
    Bacterial
    Mutation
    M225I
    Promoter
    T7-promoter
    Available Since
    Oct. 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    227219-plasmid-map-sequence-id-449537

    Enlarge

  7. K420E USP7 catalytic

    Plasmid
    #227210
    Purpose
    Expresses human USP7 Catalytic domain with a N-terminal cleavable 6His tag in E. coli
    Depositor
    Irina Bezsonova
    Insert
    USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
    Tags
    6His
    Expression
    Bacterial
    Mutation
    K420E; aa 207-560
    Promoter
    T7-promoter
    Available Since
    Oct. 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    227210-plasmid-map-sequence-id-449536

    Enlarge

  8. USP7 G392D catalytic

    Plasmid
    #227203
    Purpose
    Expresses human USP7 Catalytic domain with a N-terminal cleavable 6His tag in E. coli
    Depositor
    Irina Bezsonova
    Insert
    USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
    Tags
    6His
    Expression
    Bacterial
    Mutation
    G392D; aa 207-560
    Promoter
    T7-promoter
    Available Since
    Oct. 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    227203-plasmid-map-sequence-id-449535

    Enlarge

  9. F118L USP7 TRAF

    Plasmid
    #227228
    Purpose
    Expresses human TRAF-like domain of USP7 with a N-terminal cleavable 6His tag in E. coli
    Depositor
    Irina Bezsonova
    Insert
    USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
    Tags
    6His
    Expression
    Bacterial
    Mutation
    F118L; aa 62-205
    Promoter
    T7-promoter
    Available Since
    Oct. 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    227228-plasmid-map-sequence-id-449534

    Enlarge

  10. L469R FL USP7

    Plasmid
    #227225
    Purpose
    Expresses human USP7 with a N-terminal cleavable 6His tag in E. coli
    Depositor
    Irina Bezsonova
    Insert
    USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
    Tags
    6His
    Expression
    Bacterial
    Mutation
    L469R
    Promoter
    T7-promoter
    Available Since
    Oct. 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    227225-plasmid-map-sequence-id-449533

    Enlarge

  11. E345K USP7 catalytic

    Plasmid
    #227211
    Purpose
    Expresses human USP7 Catalytic domain with a N-terminal cleavable 6His tag in E. coli
    Depositor
    Irina Bezsonova
    Insert
    USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
    Tags
    6His
    Expression
    Bacterial
    Mutation
    E345K; aa 207-560
    Promoter
    T7-promoter
    Available Since
    Oct. 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    227211-plasmid-map-sequence-id-449531

    Enlarge

  12. L373F USP7 catalytic

    Plasmid
    #227207
    Purpose
    Expresses human USP7 Catalytic domain with a N-terminal cleavable 6His tag in E. coli
    Depositor
    Irina Bezsonova
    Insert
    USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
    Tags
    6His
    Expression
    Bacterial
    Mutation
    L373F; aa 207-560
    Promoter
    T7-promoter
    Available Since
    Oct. 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    227207-plasmid-map-sequence-id-449530

    Enlarge

  13. G392A USP7 catalytic

    Plasmid
    #227204
    Purpose
    Expresses human USP7 Catalytic domain with a N-terminal cleavable 6His tag in E. coli
    Depositor
    Irina Bezsonova
    Insert
    USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
    Tags
    6His
    Expression
    Bacterial
    Mutation
    G392A; aa 207-560
    Promoter
    T7-promoter
    Available Since
    Oct. 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    227204-plasmid-map-sequence-id-449529

    Enlarge

  14. E345K FL USP7

    Plasmid
    #227224
    Purpose
    Expresses human USP7 with a N-terminal cleavable 6His tag in E. coli
    Depositor
    Irina Bezsonova
    Insert
    USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
    Tags
    6His
    Expression
    Bacterial
    Mutation
    E345K
    Promoter
    T7-promoter
    Available Since
    Oct. 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    227224-plasmid-map-sequence-id-449528

    Enlarge

  15. V485G FL USP7

    Plasmid
    #227222
    Purpose
    Expresses human USP7 with a N-terminal cleavable 6His tag in E. coli
    Depositor
    Irina Bezsonova
    Insert
    USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
    Tags
    6His
    Expression
    Bacterial
    Mutation
    V485G
    Promoter
    T7-promoter
    Available Since
    Oct. 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    227222-plasmid-map-sequence-id-449527

    Enlarge

  20. pSCL.040

    Plasmid
    #184974
    Purpose
    Test effect of extending a1/a2 on ADE2 editing rates in yeast
    Depositor
    Seth Shipman
    Article
    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d
    Insert
    Eco1 editing ncRNA and gRNA, ADE2 P272X, a1/a2 length: 27 v2
    Expression
    Yeast
    Mutation
    ADE2 donor P272stop, a1/a2 length extended to 27 …
    Promoter
    Gal7
    Available Since
    Nov. 4, 2022
    Availability
    Academic Institutions and Nonprofits only
    184974-plasmid-map-sequence-id-376705

    Enlarge

  21. pSCL.109

    Plasmid
    #184978
    Purpose
    Test effect of extending a1/a2 on LYP1 editing rates in yeast
    Depositor
    Seth Shipman
    Article
    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d
    Insert
    Eco1 editing ncRNA and gRNA, LYP1 E27X, a1/a2 length: 27 v1
    Expression
    Yeast
    Mutation
    LYP1 donor E27stop, a1/a2 length extended to 27 bp
    Promoter
    Gal7
    Available Since
    Nov. 4, 2022
    Availability
    Academic Institutions and Nonprofits only
    184978-plasmid-map-sequence-id-376679

    Enlarge

  22. pSCL.107

    Plasmid
    #184976
    Purpose
    Test effect of extending a1/a2 on CAN1 editing rates in yeast
    Depositor
    Seth Shipman
    Article
    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d
    Insert
    Eco1 editing ncRNA and gRNA, CAN1 G444X, a1/a2 length: 27 v1
    Expression
    Yeast
    Mutation
    CAN1 donor G444stop, a1/a2 length extended to 27 …
    Promoter
    Gal7
    Available Since
    Nov. 4, 2022
    Availability
    Academic Institutions and Nonprofits only
    184976-plasmid-map-sequence-id-376676

    Enlarge

  23. pSCL.111

    Plasmid
    #184980
    Purpose
    Test effect of extending a1/a2 on TRP2 editing rates in yeast
    Depositor
    Seth Shipman
    Article
    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d
    Insert
    Eco1 editing ncRNA and gRNA, TRP2 E64X, a1/a2 length: 27 v1
    Expression
    Yeast
    Mutation
    TRP2 donor E64stop, a1/a2 length extended to 27 bp
    Promoter
    Gal7
    Available Since
    Nov. 4, 2022
    Availability
    Academic Institutions and Nonprofits only
    184980-plasmid-map-sequence-id-376669

    Enlarge

  24. pSCL.113

    Plasmid
    #184982
    Purpose
    Test effect of extending a1/a2 on FAA1 editing rates in yeast
    Depositor
    Seth Shipman
    Article
    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d
    Insert
    Eco1 editing ncRNA and gRNA, FAA1 P233X, a1/a2 length: 27 v1
    Expression
    Yeast
    Mutation
    FAA1 donor P233stop, a1/a2 length extended to 27 …
    Promoter
    Gal7
    Available Since
    Nov. 2, 2022
    Availability
    Academic Institutions and Nonprofits only
    184982-plasmid-map-sequence-id-376532

    Enlarge

  25. pSCL.039

    Plasmid
    #184973
    Purpose
    Test effect of extending a1/a2 on ADE2 editing rates in yeast
    Depositor
    Seth Shipman
    Article
    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d
    Insert
    Eco1 editing ncRNA and gRNA, ADE2 P272X, a1/a2 length: 27 v1
    Expression
    Yeast
    Mutation
    ADE2 donor P272stop, a1/a2 length extended to 27 …
    Promoter
    Gal7
    Available Since
    Nov. 2, 2022
    Availability
    Academic Institutions and Nonprofits only
    184973-plasmid-map-sequence-id-376529

    Enlarge

  36. pPP381

    Plasmid
    #158798
    Purpose
    pRSF-Duet1 derived plasmid containing C-terminally hexa-histidine tagged dCasΦ-2(D394A) in MCS1 for expression in E. coli and protein purification
    Depositor
    Jennifer Doudna
    Article
    Pausch et al Science. 2020 Jul 17;369(6501):333-337.
    Insert
    dCasPhi-2
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    RuvC-inactivating mutation D394A
    Available Since
    Aug. 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    158798-plasmid-map-sequence-id-309523

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  37. pET21b(+)-Is-PETase

    Plasmid
    #112202
    Purpose
    pET-21b(+) based plasmid for expression of PETase from Ideonella sakaiensis 201-F6 (Genbank GAP38373.1), codon optimized for expression in E. coli K12
    Depositor
    Gregg Beckham, Christopher Johnson
    Article
    Austin et al Proc Natl Acad Sci U S A. 2018 May 8;115
    Insert
    PETase gene from Ideonella sakaiensis 201-F6, codon optimized for expression in E. coli K12
    Tags
    6XHIS
    Expression
    Bacterial
    Promoter
    N/A
    Available Since
    July 18, 2018
    Availability
    Academic Institutions and Nonprofits only
    112202-plasmid-map-sequence-id-334619

    Enlarge

  39. pIRE4-Bpa

    Plasmid
    #155342
    Purpose
    Plasmid encoding the translational pair that enables efficient incorporation of Benzoyl-phenylalanine in response to the amber codon into proteins in mammmalian cells.
    Depositor
    Irene Coin
    Article
    Bottke et al EMBO Rep. 2020 Sep 14:e50437. doi: 10.15
    Insert
    humanized EBpaRS/BstYam
    Expression
    Mammalian
    Promoter
    PGK/U6
    Available Since
    Sept. 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    155342-plasmid-map-sequence-id-310519

    Enlarge

Showing: 1361 - 1400 of 1793 results