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pW212-lenti-spsgRNA-Esp3I-2kb-filler-pEF1s-NLS-mScarlet-I-P2A-BlastR

Plasmid

#170810

Purpose

Lentiviral vector to co-express an spsgRNA with NLS-mScarlet-I

Depositor

Kenneth Yamada

Article

Wang et al Cell. 2021 Jul 8;184(14):3702-3716.e30.

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Mutation

NA

Available Since

June 8, 2021

Availability

Academic Institutions and Nonprofits only

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PCDH-CMV-MCS-EF1a-gfp-T2A-puro_HSALNT0217290

Plasmid

#197989

Purpose

For the overexpression experiments, full length of the transcript (HSALNT0217290) for HSALNG0104472 was inserted.

Depositor

Bo Wang

Article

Lu et al Commun Biol. 2023 Feb 17;6(1):189. doi:

Insert

HSALNT0217290

Use

Lentiviral

Tags

lac

Expression

Mammalian

Available Since

Nov. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-SENP2 (catalytic domain)

Plasmid

#16357

Depositor

Guy Salvesen

Article

Mikolajczyk et al J Biol Chem. 2007 Sep 7. 282(36):26217-2

Insert

human SENP2 catalytic domain (SENP2 Human)

Tags

His

Expression

Bacterial

Mutation

Amino Acid 365 to 590 catalytic domain of human S…

Available Since

Dec. 19, 2007

Availability

Academic Institutions and Nonprofits only

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pMT-ADARcd-E488Q-V5

Plasmid

#139686

Purpose

Expresses only the hyperactive form of the ADAR catalytic domain - no RNA binding protein. This is a control plasmid to be used with pMT-Hrp48-ADARcd-E488Q-V5

Depositor

Michael Rosbash

Article

Xu et al RNA. 2018 Feb;24(2):173-182. doi: 10.126

Insert

Adenosine deaminase acting on RNA (Adar Fly)

Tags

V5

Expression

Bacterial and Insect

Available Since

March 11, 2020

Availability

Academic Institutions and Nonprofits only

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pRSF-G1mCNPRS

Plasmid

#174718

Purpose

tRNA synthetase/tRNA pair for the in vivo incorporation of L-3-(2-cyano-4-pyridyl)alanine (mCNP), into proteins in E. coli in response to the amber (TAG) codon

Depositor

Thomas Huber

Article

Abdelkader et al Angew Chem Int Ed Engl. 2022 Jan 31. doi

Insert

tRNA synthetase

Expression

Bacterial

Promoter

T7

Available Since

Feb. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pEF.DEST51-mScarlet

Plasmid

#207960

Purpose

Vector for overexpression of the mScarlet fluorescent protein

Depositor

Johanna Ivaska

Article

Conway et al Proc Natl Acad Sci U S A. 2023 Oct 24;12

Insert

mScarlet

Expression

Mammalian

Promoter

EF1a

Available Since

Dec. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pC003 - LshC2C2 locus into pACYC184 for spacer cloning

Plasmid

#79152

Purpose

LshC2c2 locus in pACYC184 with BsaI sites for spacer cloning

Depositor

Feng Zhang

Article

Abudayyeh et al Science. 2016 Jun 2. pii: aaf5573.

Insert

LshC2c2 locus

Mutation

*

Available Since

June 29, 2016

Availability

Academic Institutions and Nonprofits only

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pCS2-3HA-Mefv iso1

Plasmid

#92105

Purpose

Expression in eukaryotic cells

Depositor

Feng Shao

Article

Gao et al Proc Natl Acad Sci U S A. 2016 Aug 16;11

Insert

Mefv isoform1 (Mefv Mouse)

Tags

3xHA

Expression

Mammalian

Available Since

May 30, 2018

Availability

Academic Institutions and Nonprofits only
pET28a-HsSENP2

Plasmid

#71466

Purpose

Encodes human full length SENP2 optimised for E. coli expression. Suitable for in vitro transcription/translation (T7).

Depositor

Jorge Eduardo Azevedo

Article

Mendes et al Biochim Biophys Acta. 2015 Oct 29. pii:

Insert

SUMO1/sentrin specific peptidase 2 (SENP2 Human)

Tags

Hexahistidine

Expression

Bacterial

Mutation

Gene optimised for E. coli expression (Genscript)

Promoter

T7

Available Since

Dec. 10, 2015

Availability

Academic Institutions and Nonprofits only
phMYT1L-N106

Plasmid

#60861

Purpose

Expresses MYT1L under the EF1a promoter

Depositor

Andrew Yoo

Article

Victor et al Neuron. 2014 Oct 22;84(2):311-23. doi: 1

Insert

MYT1L (MYT1L Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Dec. 5, 2014

Availability

Academic Institutions and Nonprofits only

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MMLV-RT(dRH) for Split-PE (pEH50)

Plasmid

#190106

Purpose

Expresses MMLV-RT (from PE2) with truncated RNAse H domain (dRH) for Split-PE use

Depositor

Keith Joung

Article

Grunewald et al Nat Biotechnol. 2023 Mar;41(3):337-343.

Insert

MMLV-RT(delta RNAseH)

Tags

bpNLS

Expression

Mammalian

Mutation

mutations from RT in PE2 and truncation of RNAse …

Promoter

CMV

Available Since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pHR-CLIP-PD-L2

Plasmid

#223587

Purpose

Lentiviral expression of human PD-L2 with CLIP tag in mammalian cells

Depositor

Enfu Hui

Article

Zhao et al Immunity. 2019 Dec 17;51(6):1059-1073.e9

Insert

PD-L2 (PDCD1LG2 Human)

Use

Lentiviral; mammalian expression

Tags

CLIP

Available Since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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KHBD00727

Plasmid

#39748

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG9932 (CG9932 Fly)

Use

Gateway donr vector

Promoter

none

Available Since

Sept. 18, 2012

Availability

Academic Institutions and Nonprofits only
RBD#4L

Plasmid

#193021

Purpose

hnRNP C RRM RNA binding domain inserted in between N415 and N416 of Loop 1 of LwaCas13a

Depositor

Xue Gao

Article

Yang et al Nat Chem Biol. 2022 Sep 22. pii: 10.1038

Insert

Cas13a

Tags

hnRNP C RRM

Expression

Bacterial

Available Since

Dec. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-DIO-hM3D(Gq)-mCherry-shLacZ-CWB

Plasmid

#223660

Purpose

Expresses hM3D(Gq) and a control shRNA (targetting LacZ) in a Cre-dependent manner

Depositor

Oscar Marin

Article

Selten et al Nature. 2025 Apr 30. doi: 10.1038/s41586

Insert

hM3D(Gq)-mCherry and shLacZ

Use

AAV and RNAi

Tags

mCherry

Expression

Mammalian

Promoter

hSyn

Available Since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

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pHR CD19-PI3K CAR

Plasmid

#113018

Purpose

for lentivirus production of CD19-PI3K CAR

Depositor

Ron Vale

Article

Morrissey et al Elife. 2018 Jun 4;7. pii: 36688. doi: 10

Insert

CD19 CAR-P intracellular PI3k recruiting domain from CD19 (CD19 Human, Synthetic)

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Available Since

Feb. 28, 2019

Availability

Academic Institutions and Nonprofits only

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CHMP6_GFP

Plasmid

#31806

Depositor

Daniel Gerlich

Article

Guizetti et al Science. 2011 Mar 25;331(6024):1616-20.

Insert

CHMP6 (CHMP6 Human)

Expression

Mammalian

Available Since

Oct. 5, 2011

Availability

Academic Institutions and Nonprofits only

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pEM3_Euk_2NLS-Flex-Cas12a (LbCas12a)-2NLS

Plasmid

#244838

Purpose

Mammalian expression of Flex-Cas12a with 2xSV40 NLS at N-terminus and 2xSV40 at C-terminus

Depositor

Jennifer Doudna

Article

Ma et al Nucleic Acids Res. 2025 Jul 8;53(13):gka

Insert

Flex-Cas12a

Use

CRISPR

Tags

2xSV40 NLS

Expression

Mammalian

Mutation

G146R/R182V/D535G/S551F/D665N/E795Q

Promoter

T7

Available Since

Sept. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pX330_DRS-1 sgRNA / hSpCas9

Plasmid

#172841

Purpose

Mammalian expression of the DRS-1 synthetic sgRNA sequence under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.

Depositor

Haining Zhong

Article

Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

Insert

DRS-1 sgRNA under the U6 promotor and hSpCas9 under the CAG promotor

Use

CRISPR

Expression

Mammalian

Available Since

Aug. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV_SynP_DIO_tAKARα _WPRE

Plasmid

#196675

Purpose

AAV plasmid for mammalian Cre-dependent expression of tAKARα (genetically encoded sensor for protein kinase A).

Depositor

Haining Zhong

Article

Ma et al Nature. 2022 Nov;611(7937):762-768. doi:

Insert

tAKARα

Use

AAV and Cre/Lox

Expression

Mammalian

Available Since

Feb. 21, 2023

Availability

Academic Institutions and Nonprofits only

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Dynamitin-GFP

Plasmid

#51419

Purpose

expression of GFP-tagged wild-type dynamitin p50 in mammalian cells

Depositor

Trina Schroer

Article

Quintyne et al J Cell Biol. 1999 Oct 18;147(2):321-34.

Insert

Dynamitin (DCTN2 Chicken)

Tags

GFP and myc

Expression

Mammalian

Promoter

CMV

Available Since

March 28, 2017

Availability

Academic Institutions and Nonprofits only

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p2CT-His-MBP-Lbu_C2c2_R472A_H477A_R1048A_H1053A

Plasmid

#83485

Purpose

Bacterial expression of L. buccalis C2c2 CRISPR effector (double HEPN nuclease 1 and 2 inactive mutant).

Depositor

Jennifer Doudna

Article

East-Seletsky et al Nature. 2016 Sep 26. doi: 10.1038/nature

Insert

Lbu_C2c2_R472A_H477A_R1048A_H1053A

Use

CRISPR

Tags

His6-MBP

Expression

Bacterial

Mutation

R472A, H477A, R1048A, H1053A

Available Since

Oct. 31, 2016

Availability

Academic Institutions and Nonprofits only

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PA-Cdc42

Plasmid

#75263

Purpose

Light-activated Cdc42

Depositor

Klaus Hahn

Article

Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

Insert

Cell Division Cycle 42 (CDC42 Human)

Tags

6xHIs, LOV2, and mVenus

Expression

Bacterial, Insect, and Mamm…

Mutation

F56W

Promoter

CMV

Available Since

July 26, 2016

Availability

Academic Institutions and Nonprofits only

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mCit-4aa-SNAP25B

Plasmid

#53235

Purpose

Fluorescent probe: SNAP-25B, N-terminally-tagged with monomeric Citrine, connected with a 4 amino acid linker. Expressed in mammalian cells, used with mCer-4aa-VAMP2 to measure FRET and dispersion.

Depositor

Robert Zucker

Article

Degtyar et al J Neurosci. 2013 Mar 27;33(13):5507-23.

Insert

SNAP-25B (Snap25 Rat)

Tags

4aa-linker and mCitrine

Expression

Mammalian

Available Since

June 9, 2014

Availability

Academic Institutions and Nonprofits only

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pUCHR-osTIR1-IRES-GFP

Plasmid

#110661

Purpose

Lentiviral vector for stable expression of osTIR1 and selection with GFP

Depositor

Dmitriy Mazurov

Article

Zotova et al Sci Rep. 2019 Feb 28;9(1):3132. doi: 10.

Insert

osTIR1

Use

Lentiviral

Promoter

CMV

Available Since

March 1, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-COX4-dAPEX2

Plasmid

#117176

Purpose

Plasmid name in publication: pAAV-Matrix-dAPEX2. Peroxidase reporter for multiplexed electron microscopy labeling

Depositor

David Ginty

Article

Zhang et al Nat Neurosci. 2019 Mar 18. pii: 10.1038/

Insert

COX4-dAPEX2

Use

AAV

Expression

Mammalian

Mutation

W41F and A134P on soybean APX

Promoter

EF1a

Available Since

March 19, 2019

Availability

Academic Institutions and Nonprofits only

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gfp-Arkadia/RNF111

Plasmid

#112228

Purpose

Expresses GFP-tagged Arkadia in mammalian cells

Depositor

Vasso Episkopou

Article

Mavrakis et al PLoS Biol. 2007 Mar;5(3):e67. doi: 10.13

Insert

(Arkadia)Rnf111 (Rnf111 Mouse)

Tags

GFP

Expression

Mammalian

Available Since

July 12, 2018

Availability

Academic Institutions and Nonprofits only

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pCJR01

Plasmid

#187888

Purpose

Escherichia coli plasmid with cloned regions of homology to Campylobacter jejuni chromosome flanking a NotI restriction site. pCJR01 functions as a suicide vector for integrating cloned NotI DNA.

Depositor

Gaylen Uhlich

Article

Uhlich et al Biotechniques. 2022 Apr 13. doi: 10.2144

Insert

DNA insert has homology to a rRNA region of the Campylobacter jejuni chromosome with an added central NotI restriction site

Expression

Bacterial

Promoter

none

Available Since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pUASTattB-dALFANb(5mut)-mGreenLantern

Plasmid

#219661

Purpose

Gal4-inducible expression of the destabilised anti-ALFAtag nanobody (including 5 destabilising mutations) fused to mGreenLantern

Depositor

Markus Affolter

Article

Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

Insert

Destabilised ALFA nanobody (5 mutations) fused to mGreenLantern

Expression

Insect

Available Since

Nov. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pUC57-Amp_CRISPIE donor B1

Plasmid

#172842

Purpose

CRISPIE donor B1 (Zhong et al, eLife 2021), mEGFP translational phase (0-0), excised by ACTB i4 sgRNA or DRS-1 sgRNA (with SpCas9).

Depositor

Haining Zhong

Article

Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

Insert

CRISPIE designer exon (phase 0-0) encoding mEGFP

Use

CRISPR

Available Since

Aug. 24, 2021

Availability

Academic Institutions and Nonprofits only

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