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Plasmid#167920PurposeLentiviral vector for expressing U6 FE-sgRNA cloning plasmidDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceFeb. 9, 2022AvailabilityAcademic Institutions and Nonprofits only
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pSB700 lenti gRNA EYFP_TtoC and hairpin extension
Plasmid#167922PurposeLentiviral vector for expressing U6 FE-sgRNA cloning plasmidDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceFeb. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSB700 lenti gRNA Hygro_TtoC and hairpin extension
Plasmid#167923PurposeLentiviral vector for expressing U6 FE-sgRNA cloning plasmidDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceFeb. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSB700 lenti gRNA iRFP713_TtoC and hairpin extension
Plasmid#167924PurposeLentiviral vector for expressing U6 FE-sgRNA cloning plasmidDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceFeb. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSB700 lenti gRNA KanR neo_TtoC and hairpin extension
Plasmid#167925PurposeLentiviral vector for expressing U6 FE-sgRNA cloning plasmidDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceFeb. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSB700 lenti gRNA Puro_TtoC and hairpin extension
Plasmid#167927PurposeLentiviral vector for expressing U6 FE-sgRNA cloning plasmidDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceFeb. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
NAX_CAGGS_CAS9_m4_KRAB-MeCP2_Blasto
Plasmid#167896PurposePiggyBac compatible plasmid expressing dCas9-KRAB-MeCP2 expression plasmidDepositorInsertdCas9-KRAB-MeCP2
UseCRISPRTagsExpressionMutationD10A, D839A, H840A, N863APromoterAvailable sinceFeb. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
NAX_CAGGS_CAS9_m4_VPR_iRFP713
Plasmid#167884PurposePiggyBac compatible plasmid expressing dCas9-VPRDepositorInsertdCas9-VPR
UseCRISPRTagsExpressionMutationD10A, D839A, H840A, N863APromoterAvailable sinceFeb. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
NAX_CAGGS_CAS9_m4_KRAB_Puro
Plasmid#167879PurposePiggyBac compatible plasmid expressing dCas9-KRABDepositorInsertdCas9-KRAB
UseCRISPRTagsExpressionMutationD10A, D839A, H840A, N863APromoterAvailable sinceFeb. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
NAX_CAGGS_CAS9_m4_KRAB_iRFP713
Plasmid#167876PurposePiggyBac compatible plasmid expressing dCas9-KRABDepositorInsertdCas9-KRAB
UseCRISPRTagsExpressionMutationD10A, D839A, H840A, N863APromoterAvailable sinceFeb. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
NAX_CAGGS_CAS9_m4_KRAB_Hygro
Plasmid#167875PurposePiggyBac compatible plasmid expressing dCas9-KRABDepositorInsertdCas9-KRAB
UseCRISPRTagsExpressionMutationD10A, D839A, H840A, N863APromoterAvailable sinceFeb. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
NAX_CAGGS_CAS9_m4_KRAB_EYFP
Plasmid#167874PurposePiggyBac compatible plasmid expressing dCas9-KRABDepositorInsertdCas9-KRAB
UseCRISPRTagsExpressionMutationD10A, D839A, H840A, N863APromoterAvailable sinceFeb. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
NAX_CAGGS_CAS9_m4_KRAB_Blasto
Plasmid#167872PurposePiggyBac compatible plasmid expressing dCas9-KRABDepositorInsertdCas9-KRAB
UseCRISPRTagsExpressionMutationD10A, D839A, H840A, N863APromoterAvailable sinceFeb. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pMC6-spacer
Plasmid#176178Purposeyeast MoClo level-0 part plasmid type 6 containing non-coding DNA spacer for construction of MoClo plasmids without yeast markerDepositorInsert41 bp non-coding DNA spacer from pYTK048
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET29b-AviTag-mEGFP-dspB(E184Q W330Y)-6xHis
Plasmid#176575PurposePlasmid for overexpression of recombinant AviTagged, His-tagged fusion protein mEGFP-DspB(E184Q W330Y), used as a non-binding control for detection of biofilm polysaccharide PNAG.DepositorInsertDispersin B
UseTagsAvitag, Hexahistidine tag, and mEGFPExpressionBacterialMutationE184Q W330YPromoterT7Available sinceNov. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBbdCas9S(-10AC1)_Psyn-sgRNArodA
Plasmid#149659Purposeall-in-one CRISPRi vector for targeting B. burgdorferi rodADepositorInsertdCas9, lacI, sgRNArodA
UseCRISPRTagsExpressionBacterialMutationPromoterPflaB, PpQE30(-10AC1), PsynAvailable sinceNov. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBbdCas9S(-10AC2)_Psyn-sgRNArodA
Plasmid#149660Purposeall-in-one CRISPRi vector for targeting B. burgdorferi rodADepositorInsertdCas9, lacI, sgRNArodA
UseCRISPRTagsExpressionBacterialMutationPromoterPflaB, PpQE30(-10AC2), PsynAvailable sinceNov. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBbdCas9S(-10TC)_Psyn-sgRNAmreB
Plasmid#149595Purposeall-in-one CRISPRi vector for targeting B. burgdorferi mreBDepositorInsertdCas9, lacI, sgRNAmreB
UseTagsExpressionBacterialMutationPromoterPflaB, PpQE30(-10TC), PsynAvailable sinceNov. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBbdCas9S(-10TC)_Psyn-sgRNAftsI
Plasmid#149591Purposeall-in-all CRISPRi vector for targeting B. burgdorferi ftsIDepositorInsertdCas9, lacI, sgRNAftsI
UseCRISPRTagsExpressionBacterialMutationPromoterPflaB, PpQE30(-10TC), PsynAvailable sinceNov. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBbdCas9S(-10TC)_Psyn-sgRNArodA
Plasmid#149657Purposeall-in-one CRISPRi vector for targeting B. burgdorferi rodADepositorInsertdCas9, lacI, sgRNArodA
UseCRISPRTagsExpressionBacterialMutationPromoterPflaB, PpQE30(-10TC), PsynAvailable sinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBbdCas9S(-10TC)_Psyn-sgRNAflaB
Plasmid#149589Purposeall-in-one CRISPRi vector for targeting B. burgdorferi flaBDepositorInsertdCas9, lacI, sgRNAflaB
UseTagsExpressionBacterialMutationPromoterPflaB, PpQE30(-10TC), PsynAvailable sinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pC-KIT3
Plasmid#118985PurposeMutational dissection of the G rich region of the c-kit promoterDepositorInsertc-kit proto-oncogene {promoter} (KIT Human)
UseTagsExpressionMammalianMutationK2-mutSP-K1PromoterAvailable sinceJune 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pC-KIT7
Plasmid#118989PurposeMutational dissection of the G rich region of the c-kit promoterDepositorInsertc-kit proto-oncogene {promoter} (KIT Human)
UseTagsExpressionMammalianMutationK2-mutSP-mutK1PromoterAvailable sinceJune 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
TIAM2_PDZ_1
Plasmid#103952PurposeProtein expression and purification of TIAM2 PDZ domainDepositorInsertTIAM2 (TIAM2 Human)
UseTagsGSTExpressionBacterialMutationPromotertacAvailable sinceAug. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
SNTA1_PDZ_1
Plasmid#103928PurposeProtein expression and purification of STNA1 PDZ domainDepositorInsertSNTA1 (SNTA1 Human)
UseTagsGSTExpressionBacterialMutationPromotertacAvailable sinceAug. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
MPP1_PDZ_1
Plasmid#103931PurposeProtein expression and purification of MPP1 PDZ domainDepositorInsertMPP1 (MPP1 Human)
UseTagsGSTExpressionBacterialMutationPromotertacAvailable sinceAug. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
PDZK1_PDZ_2
Plasmid#103943PurposeProtein expression and purification of PDZK1 PDZ2 domainDepositorInsertPDZK1 (PDZK1 Human)
UseTagsGSTExpressionBacterialMutationPromotertacAvailable sinceAug. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
PDZK1_PDZ_3
Plasmid#103944PurposeProtein expression and purification of PDZK1 PDZ3 domainDepositorInsertPDZK1 (PDZK1 Human)
UseTagsGSTExpressionBacterialMutationPromotertacAvailable sinceAug. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
MPP6_PDZ_1
Plasmid#103924PurposeProtein expression and purification of P55 PDZ domainDepositorInsertMPP6 (PALS2 Human)
UseTagsGSTExpressionBacterialMutationPromotertacAvailable sinceAug. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
INADL_PDZ_5
Plasmid#103905PurposeProtein expression and purification of INADL PDZ5 domainDepositorInsertINADL (PATJ Human)
UseTagsGSTExpressionBacterialMutationPromotertacAvailable sinceAug. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
INADL_PDZ_6
Plasmid#103906PurposeProtein expression and purification of INADL PDZ6 domainDepositorInsertINADL (PATJ Human)
UseTagsGSTExpressionBacterialMutationPromotertacAvailable sinceAug. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
INADL_PDZ_7
Plasmid#103907PurposeProtein expression and purification of INADL PDZ7 domainDepositorInsertINADL (PATJ Human)
UseTagsGSTExpressionBacterialMutationPromotertacAvailable sinceAug. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
SCRIB_PDZ_2
Plasmid#103884PurposeProtein expression and purification of Scribble PDZ2 domainDepositorInsertSCRIB (SCRIB Human)
UseTagsGSTExpressionBacterialMutationPromotertacAvailable sinceAug. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
MPDZ_PDZ_1
Plasmid#103885PurposeProtein expression and purification of Multiple PDZ protein PDZ1 domainDepositorInsertMPDZ (MPDZ Human)
UseTagsGSTExpressionBacterialMutationPromotertacAvailable sinceAug. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
MPDZ_PDZ_2
Plasmid#103886PurposeProtein expression and purification of Multiple PDZ protein PDZ2 domainDepositorInsertMPDZ (MPDZ Human)
UseTagsGSTExpressionBacterialMutationPromotertacAvailable sinceAug. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
MPDZ_PDZ_4
Plasmid#103888PurposeProtein expression and purification of Multiple PDZ protein PDZ4 domainDepositorInsertMPDZ (MPDZ Human)
UseTagsGSTExpressionBacterialMutationPromotertacAvailable sinceAug. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
MPDZ_PDZ_5
Plasmid#103889PurposeProtein expression and purification of Multiple PDZ protein PDZ5 domainDepositorInsertMPDZ (MPDZ Human)
UseTagsGSTExpressionBacterialMutationPromotertacAvailable sinceAug. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
MPDZ_PDZ_6
Plasmid#103890PurposeProtein expression and purification of Multiple PDZ protein PDZ6 domainDepositorInsertMPDZ (MPDZ Human)
UseTagsGSTExpressionBacterialMutationPromotertacAvailable sinceAug. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
MPDZ_PDZ_8
Plasmid#103892PurposeProtein expression and purification of Multiple PDZ protein PDZ8 domainDepositorInsertMPDZ (MPDZ Human)
UseTagsGSTExpressionBacterialMutationPromotertacAvailable sinceAug. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
MPDZ_PDZ_9
Plasmid#103893PurposeProtein expression and purification of Multiple PDZ protein PDZ9 domainDepositorInsertMPDZ (MPDZ Human)
UseTagsGSTExpressionBacterialMutationPromotertacAvailable sinceAug. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
MPDZ_PDZ_12
Plasmid#103896PurposeProtein expression and purification of Multiple PDZ protein PDZ12 domainDepositorInsertMPDZ (MPDZ Human)
UseTagsGSTExpressionBacterialMutationPromotertacAvailable sinceAug. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
INADL_PDZ_2
Plasmid#103902PurposeProtein expression and purification of INADL PDZ2 domainDepositorInsertINADL (PATJ Human)
UseTagsGSTExpressionBacterialMutationPromotertacAvailable sinceAug. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
INADL_PDZ_3
Plasmid#103903PurposeProtein expression and purification of INADL PDZ3 domainDepositorInsertINADL (PATJ Human)
UseTagsGSTExpressionBacterialMutationPromotertacAvailable sinceAug. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
INADL_PDZ_4
Plasmid#103904PurposeProtein expression and purification of INADL PDZ4 domainDepositorInsertINADL (PATJ Human)
UseTagsGSTExpressionBacterialMutationPromotertacAvailable sinceAug. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGS_C_3xFLAG
Plasmid#101653PurposeExpression of type I signal-peptide containing proteins with C-terminal 3xFLAG-tag in insect cellsDepositorInsertLRP6 Signal peptide, MCS linker, 3xFLAG tag
UseTags3xFLAG tagExpressionInsectMutationPromoterAvailable sinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
BB1_34_chr4_0883tt
Plasmid#98524PurposeBB1 with insert: chr4_0883ttDepositorInsertchr4_0883tt
UseTagsExpressionBacterialMutationInternal BsaI and BpiI sites removed from insertPromoterAvailable sinceFeb. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
BB1_34_IDP1tt
Plasmid#98525PurposeBB1 with insert: IDP1ttDepositorInsertIDP1tt
UseTagsExpressionBacterialMutationInternal BsaI and BpiI sites removed from insertPromoterAvailable sinceFeb. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
BB1_34_RPS3tt
Plasmid#98527PurposeBB1 with insert: RPS3ttDepositorInsertRPS3tt
UseTagsExpressionBacterialMutationInternal BsaI and BpiI sites removed from insertPromoterAvailable sinceFeb. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
BB1_34_RPL2Att
Plasmid#98528PurposeBB1 with insert: RPL2AttDepositorInsertRPL2Att
UseTagsExpressionBacterialMutationInternal BsaI and BpiI sites removed from insertPromoterAvailable sinceFeb. 20, 2018AvailabilityAcademic Institutions and Nonprofits only