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We narrowed to 7,219 results for: cas9 plasmid

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Showing: 1751 - 1800 of 7219 results

  1. OgeuIscB GCA RNA

    Plasmid
    #222864
    Purpose
    This plasmid codes for the guide of the OgeuIscB with a GCA target
    Depositor
    Vincent Dion
    Article
    Mangin et al bioRxiv 2023.10.24.563270
    Insert
    OgeuIscB omega RNA with a (GCA)6 target sequence
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    Human U6
    Available Since
    Oct. 16, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    222864-plasmid-map-sequence-id-449452

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  2. OgeuIscB TGC RNA

    Plasmid
    #222863
    Purpose
    This plasmid codes for the guide of the OgeuIscB with a TGC target
    Depositor
    Vincent Dion
    Article
    Mangin et al bioRxiv 2023.10.24.563270
    Insert
    OgeuIscB omega RNA with a (TGC)6 target sequence
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    Human U6
    Available Since
    Oct. 16, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    222863-plasmid-map-sequence-id-449446

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  3. OgeuIscB AGC RNA

    Plasmid
    #222866
    Purpose
    This plasmid codes for the guide of the OgeuIscB with a AGC target
    Depositor
    Vincent Dion
    Article
    Mangin et al bioRxiv 2023.10.24.563270
    Insert
    OgeuIscB omega RNA with a (AGC)6 target sequence
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    Human U6
    Available Since
    Oct. 16, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    222866-plasmid-map-sequence-id-449445

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  4. OgeuIscB CAG RNA

    Plasmid
    #222867
    Purpose
    This plasmid codes for the guide of the OgeuIscB with a CAG target
    Depositor
    Vincent Dion
    Article
    Mangin et al bioRxiv 2023.10.24.563270
    Insert
    OgeuIscB omega RNA with a (CAG)6 target sequence
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    Human U6
    Available Since
    Oct. 16, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    222867-plasmid-map-sequence-id-449443

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  5. pDM052

    Plasmid
    #216814
    Purpose
    Aspergillus nidulans codon-adjusted mNeonGreen fluorescent protein, includes linker for C-terminal tagging.
    Depositor
    Ken Sawin
    Article
    Modaffari et al Wellcome Open Res. 2024 Oct 17;9:602. do
    Insert
    mNeonGreen
    Tags
    VinnyLinker
    Expression
    Bacterial
    Mutation
    Aspergillus nidulans codon-adjusted
    Available Since
    Sept. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    216814-plasmid-map-sequence-id-429539

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  6. pDM042

    Plasmid
    #216812
    Purpose
    Aspergillus nidulans codon-adjusted mScarlet3 fluorescent protein, includes linker for C-terminal tagging.
    Depositor
    Ken Sawin
    Article
    Modaffari et al Wellcome Open Res. 2024 Oct 17;9:602. do
    Insert
    mScarlet3
    Tags
    VinnyLinker
    Expression
    Bacterial
    Mutation
    Aspergillus nidulans codon-adjusted
    Available Since
    Sept. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    216812-plasmid-map-sequence-id-429538

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  9. pCAG-EGxxFP

    Plasmid
    #50716
    Purpose
    5’ and 3’ EGFP fragments that shares 482bp were placed under ubiquitous CAG promoter. Used for validation of gRNA sequences by DSB mediated EGFP reconstitution.
    Depositor
    Masahito Ikawa
    Article
    Mashiko et al Sci Rep. 2013 Nov 27;3:3355. doi: 10.103
    Insert
    split EGFP
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    Feb. 3, 2014
    Availability
    Academic Institutions and Nonprofits only
    50716-plasmid-map-sequence-id-447281

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  10. AICSDP-63: ACTN2-mEGFP

    Plasmid
    #124607
    Purpose
    Homology arms and linker-mEGFP sequence for C-terminus tagging of human ACTN2, via excisable Cas9-excisable CAGGS-mCherry selection cassette
    Depositor
    Allen Institute for Cell Science
    Insert
    ACTN2 Homology Arms with linker-mEGFP and Cas9-excisable selection cassette (ACTN2 Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Available Since
    May 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    124607-plasmid-map-sequence-id-244336

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  13. pDM043

    Plasmid
    #216813
    Purpose
    Aspergillus nidulans codon-adjusted mScarlet3 fluorescent protein, includes linker for N-terminal tagging.
    Depositor
    Ken Sawin
    Article
    Modaffari et al Wellcome Open Res. 2024 Oct 17;9:602. do
    Insert
    mScarlet3
    Tags
    VinnyLinker
    Expression
    Bacterial
    Mutation
    Aspergillus nidulans codon-adjusted
    Available Since
    Sept. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    216813-plasmid-map-sequence-id-430146

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  14. pDM067

    Plasmid
    #216820
    Purpose
    Aspergillus nidulans codon-adjusted mStayGold2 fluorescent protein, includes linker for C-terminal tagging.
    Depositor
    Ken Sawin
    Article
    Modaffari et al Wellcome Open Res. 2024 Oct 17;9:602. do
    Insert
    mStayGold2
    Tags
    VinnyLinker
    Expression
    Bacterial
    Mutation
    Aspergillus nidulans codon-adjusted
    Available Since
    Sept. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    216820-plasmid-map-sequence-id-430143

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  15. pDM069

    Plasmid
    #216821
    Purpose
    Aspergillus nidulans codon-adjusted mStayGold2 fluorescent protein, includes linker for N-terminal tagging.
    Depositor
    Ken Sawin
    Article
    Modaffari et al Wellcome Open Res. 2024 Oct 17;9:602. do
    Insert
    mStayGold2
    Tags
    RIPGLIN and VinnyLinker
    Expression
    Bacterial
    Mutation
    Aspergillus nidulans codon-adjusted
    Available Since
    Sept. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    216821-plasmid-map-sequence-id-430130

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  16. pDM053

    Plasmid
    #216815
    Purpose
    Aspergillus nidulans codon-adjusted mNeonGreen fluorescent protein, includes linker for N-terminal tagging.
    Depositor
    Ken Sawin
    Article
    Modaffari et al Wellcome Open Res. 2024 Oct 17;9:602. do
    Insert
    mNeonGreen
    Tags
    RIPGLIN
    Expression
    Bacterial
    Mutation
    Aspergillus nidulans codon-adjusted
    Available Since
    Sept. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    216815-plasmid-map-sequence-id-429382

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  17. pDM071

    Plasmid
    #216817
    Purpose
    Aspergillus nidulans codon-adjusted mCardinal fluorescent protein, includes linker for N-terminal tagging.
    Depositor
    Ken Sawin
    Article
    Modaffari et al Wellcome Open Res. 2024 Oct 17;9:602. do
    Insert
    mCardinal
    Tags
    RIPGLIN
    Expression
    Bacterial
    Mutation
    Aspergillus nidulans codon-adjusted
    Available Since
    Sept. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    216817-plasmid-map-sequence-id-430145

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  19. pDM070

    Plasmid
    #216816
    Purpose
    Aspergillus nidulans codon-adjusted mCardinal fluorescent protein, includes linker for C-terminal tagging.
    Depositor
    Ken Sawin
    Article
    Modaffari et al Wellcome Open Res. 2024 Oct 17;9:602. do
    Insert
    mCardinal
    Tags
    VinnyLinker
    Expression
    Bacterial
    Mutation
    Aspergillus nidulans codon-adjusted
    Available Since
    Sept. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    216816-plasmid-map-sequence-id-429610

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  24. p35C-gRNA-ccdB

    Plasmid
    #246792
    Purpose
    Express gRNA in plant cells (protoplast or callus) for Cas9-mediated editing
    Depositor
    Caixia Gao
    Insert
    ccdB
    Use
    CRISPR
    Available Since
    March 17, 2026
    Availability
    Academic Institutions and Nonprofits only
    246792-plasmid-map-sequence-id-490425

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  25. pORFE1001

    Plasmid
    #112079
    Purpose
    Overexpression of the AtCAS9 in plant under 2x35S promoter
    Depositor
    Christian Chevalier
    Insert
    AtCAS9
    Use
    CRISPR
    Expression
    Plant
    Mutation
    Plant codon optimized Cas9 S. pyogenes
    Promoter
    2x35S
    Available Since
    Aug. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    112079-plasmid-map-sequence-id-217610

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  26. HCP5

    Plasmid
    #166107
    Purpose
    This plasmid encodes a Cas9 protein as well as a sgRNAs that targets the C-terminus of Cyr1.
    Depositor
    Matthias Heinemann
    Insert
    Cyr1CT-sgRNA (CYR1 Budding Yeast)
    Use
    CRISPR
    Expression
    Yeast
    Available Since
    April 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    166107-plasmid-map-sequence-id-330320

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  27. GE-0010-gRNA2-MpCKB

    Plasmid
    #238528
    Purpose
    Plant expression of Cas9 and gRNA against M. polymorpha CK2 beta
    Depositor
    Michael Hothorn
    Insert
    MpCKB
    Use
    CRISPR
    Expression
    Plant
    Available Since
    June 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    238528-plasmid-map-sequence-id-477293

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  28. GE-0010-gRNA1-MpCKB

    Plasmid
    #238527
    Purpose
    Plant expression of Cas9 and gRNA against M. polymorpha CK2 beta
    Depositor
    Michael Hothorn
    Insert
    MpCKB
    Use
    CRISPR
    Expression
    Plant
    Available Since
    June 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    238527-plasmid-map-sequence-id-477292

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  29. GE-0010-gRNA1-MpCKA

    Plasmid
    #238526
    Purpose
    Plant expression of Cas9 and gRNA against M. polymorpha CK2 alpha
    Depositor
    Michael Hothorn
    Insert
    MpCKA
    Use
    CRISPR
    Expression
    Plant
    Available Since
    June 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    238526-plasmid-map-sequence-id-477280

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  30. 3xgRNA: PTEN exon 5, p53 exon 8, SMAD4 exon 2 probasin_mTQ2_FlpO

    Plasmid
    #68405
    Purpose
    The prostate specific promoter controls expression of FlpO linked to a blue flourescent protein. gRNA towards PTEN ex5, p53 ex8 and SMAD4 ex2. are expressed by the U6 promoter
    Depositor
    Jannik Elverløv-Jakobsen
    Inserts
    3xgRNA: PTEN exon 5, p53 exon 8, SMAD4 exon 2
    FlpO
    Use
    CRISPR
    Tags
    mTurquoise2 (BFP)
    Expression
    Mammalian
    Promoter
    Synthetic Probasin ARRx2 and U6
    Available Since
    Oct. 5, 2015
    Availability
    Academic Institutions and Nonprofits only
    68405-plasmid-map-sequence-id-119955

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  31. pPtGE35

    Plasmid
    #107999
    Purpose
    Expresses TevCas9 in Phaeodactylum tricornutum / Encodes elements required for conjugation
    Depositor
    David Edgell
    Article
    Slattery et al ACS Synth Biol. 2018 Jan 8. doi: 10.1021
    Inserts
    OriT
    40SRPS8 Promoter
    ShBle
    40SRPS8 Terminator
    Cen6-ArsH4-His3
    I-TevI nuclease and partial linker domain
    Use
    CRISPR and Synthetic Biology; Episomal vector for…
    Tags
    Cas9
    Available Since
    Sept. 13, 2018
    Availability
    Academic Institutions and Nonprofits only
    107999-plasmid-map-sequence-id-217151

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  33. MSP1101

    Plasmid
    #65773
    Purpose
    Human expression vector for SpCas9 VRER variant: CMV-T7-humanSpCas9(D1135V/G1218R/R1335E/T1337R)-NLS-3xFLAG (VRER variant)
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature
    Insert
    mammalian codon-optimized Streptococcus pyogenes Cas9 (D1135V/G1218R/R1335E/T1337R)-NLS-3XFlag
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Mammalian
    Mutation
    D1135V, G1218R, R1335E, and T1337R mutations in C…
    Promoter
    CMV & T7
    Available Since
    June 22, 2015
    Availability
    Academic Institutions and Nonprofits only
    65773-plasmid-map-sequence-id-112657

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  34. pAAV-CMV-dSa VP64 Neurog2

    Plasmid
    #99695
    Purpose
    Expresses dSa Cas9 fused to gold standard VP64 activator domain and Sa sgRNA for Neurog2 promoter, vector allows for activation of mouse Neurog2, can be packaged and delivered as AAV
    Depositor
    George Church
    Article
    Vora et al bioRxiv 2018. 298620
    Insert
    dCas9 and gRNA targeting Neurog2 (gRNA: GGTATATAAGGGGTTTTAAG) (Neurog2 Mouse, Synthetic)
    Use
    AAV and CRISPR
    Tags
    VP64
    Mutation
    dead Cas9
    Available Since
    Sept. 22, 2018
    Availability
    Academic Institutions and Nonprofits only
    99695-plasmid-map-sequence-id-194570

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  35. pCAG-B2UP49_GFP

    Plasmid
    #103071
    Purpose
    Cas9 coding gene template with optimized sequence for human codon usage, also expresses EGFP
    Depositor
    Duhee Bang
    Article
    Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55
    Insert
    B2UP49(Cas9 coding gene from Akkermansia muciniphila (strain ATCC BAA-835))
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    human codon-optimized
    Promoter
    CMV
    Available Since
    April 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    103071-plasmid-map-sequence-id-201896

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  36. pAAV-CMV-dSa VP64 Actc1

    Plasmid
    #99691
    Purpose
    Expresses dSa Cas9 fused to gold standard VP64 activator domain and Sa sgRNA for Actc1 promoter, vector allows for activation of mouse Actc1, can be packaged and delivered as AAV
    Depositor
    George Church
    Article
    Vora et al bioRxiv 2018. 298620
    Insert
    dCas9 and gRNA targeting Actc1 (gRNA sequence: GCCATTCTTGGAGCCAAGGG) (Actc1 Mouse, Synthetic)
    Use
    AAV and CRISPR
    Tags
    VP64
    Mutation
    dead Cas9
    Available Since
    Dec. 21, 2022
    Availability
    Academic Institutions and Nonprofits only
    99691-plasmid-map-sequence-id-194530

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  37. pLenti-CRISPRv2-sgNTC_#2-hygro

    Plasmid
    #254677
    Purpose
    knockout non-targeting control
    Depositor
    Brian Ruffell
    Insert
    sgRNA with Cas9 and hygromycin resistance
    Use
    CRISPR and Lentiviral
    Available Since
    May 4, 2026
    Availability
    Academic Institutions and Nonprofits only
    254677-plasmid-map-sequence-id-508740

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  38. pLenti-CRISPRv2-sgNTC_#1-hygro

    Plasmid
    #254676
    Purpose
    knockout non-targeting control
    Depositor
    Brian Ruffell
    Insert
    sgRNA with Cas9 and hygromycin resistance
    Use
    CRISPR and Lentiviral
    Available Since
    May 4, 2026
    Availability
    Academic Institutions and Nonprofits only
    254676-plasmid-map-sequence-id-508735

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  46. pCSD_2xNES-FRB-ZFPTS2-3xFLAG-2xNLS

    Plasmid
    #107303
    Purpose
    Expresses ZFP-VEGFA-TS2 fused to FRB in mammalian cells
    Depositor
    Scot Wolfe
    Insert
    ZFP_VEGFA-TS2
    Use
    CRISPR
    Tags
    2xNES, 3x Flag, 2xNLS, and FRB
    Expression
    Mammalian
    Promoter
    CMV IE94
    Available Since
    Nov. 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    107303-plasmid-map-sequence-id-204639

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  47. pCSD_2xNES-FRB-ZFPTS3-3xFLAG-2xNLS

    Plasmid
    #107304
    Purpose
    Expresses ZFP-VEGFA-TS3 fused to FRB in mammalian cells
    Depositor
    Scot Wolfe
    Insert
    ZFP_VEGFA-TS3
    Use
    CRISPR
    Tags
    2xNES, 3x Flag, 2xNLS, and FRB
    Expression
    Mammalian
    Promoter
    CMV IE94
    Available Since
    Nov. 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    107304-plasmid-map-sequence-id-204643

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  48. 4xgRNA: PTEN exon 5, p53 exon 8, SMAD4 exon 2, p53 exon 7 probasin_mTQ2_FlpO

    Plasmid
    #68356
    Purpose
    -The prostate specific promoter controls expression of FlpO linked to a blue flourescent protein. gRNA towards PTEN ex5, p53 ex7 and SMAD4 ex2. are expressed by the U6 promoter
    Depositor
    Jannik Elverløv-Jakobsen
    Inserts
    4xgRNA: PTEN exon 5, p53 exon 8, SMAD4 exon 2, p53 exon 7
    FlpO recombinase
    Use
    CRISPR
    Tags
    mTurquoise2 (BFP)
    Expression
    Mammalian
    Promoter
    Synthetic Probasin ARRx2 and U6
    Available Since
    Oct. 5, 2015
    Availability
    Academic Institutions and Nonprofits only
    68356-plasmid-map-sequence-id-119628

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  49. pUC gRNA Shuttle

    Plasmid
    #47024
    Purpose
    Encodes a template from which gRNAs can be made via InFusion cloning. The Medicago truncatula U6.6 promoter drives the gRNA. For use in plants.
    Depositor
    Wayne Parrott
    Article
    Jacobs et al BMC Biotechnol. 2015 Mar 12;15:16. doi:
    Insert
    gRNA Shuttle
    Use
    CRISPR; Cas9
    Expression
    Plant
    Mutation
    G to T cloning mutation at position 323
    Promoter
    Medicago truncatula U6.6
    Available Since
    Sept. 3, 2013
    Availability
    Industry, Academic Institutions, and Nonprofits
    47024-plasmid-map-sequence-id-67042

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Showing: 1751 - 1800 of 7219 results