We narrowed to 987 results for: Streptococcus

1. NLS-nCas9-NLS-P2A-EGFP (pRZ70)

   Plasmid

   #123616

   Purpose

   CMV promoter expression plasmid for codon-optimized bpNLS-32AA linker-hSpCas9n(D10A)-bpNLS-P2A-EGFP (ABEmax control without TadA domains).

   Depositor

   Keith Joung

   Article

   Grunewald et al Nature. 2019 Apr 17. pii: 10.1038/s41586

   Insert

   NLS-nCas9-NLS-P2A-EGFP
   

   Expression

   Mammalian

   Mutation

   D10A in SpCas9

   Promoter

   CMV

   Available Since

   April 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. nCas9-UGI-NLS-P2A-EGFP (pJUL1001)

   Plasmid

   #123611

   Purpose

   CAG promoter expression plasmid for hSpCas9n(D10A)-UGI-NLS(SV40)-P2A-EGFP (BE3 control without rAPOBEC1).

   Depositor

   Keith Joung

   Article

   Grunewald et al Nature. 2019 Apr 17. pii: 10.1038/s41586

   Insert

   nCas9-UGI-NLS-P2A-EGFP
   

   Expression

   Mammalian

   Mutation

   D10A in SpCas9

   Promoter

   CAG

   Available Since

   April 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pNOC_dCas9_sgRNA NC

   Plasmid

   #176259

   Purpose

   pNOC episomal plasmid harboring the dead version of humanized spCas9 gene sequence tagged with Nlux and spacer sequence on sgRNA is replaced by the type IIS restriction site for endonuclease BaeI and

   Depositor

   John van der Oost

   Article

   Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-

   Insert

   dead spCas9
   

   Use

   CRISPR, Luciferase, and Synthetic Biology ; Expre…

   Tags

   luciferase

   Mutation

   H840A and D10A mutations on spCas9 to inactivate …

   Available Since

   Oct. 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. hAID-BE3 (pRZ352)

   Plasmid

   #131316

   Purpose

   CAG promoter expression plasmid for hAID-XTEN linker-hSpCas9n(D10A)-UGI-NLS-P2A-EGFP.

   Depositor

   Keith Joung

   Article

   Grunewald et al Nat Biotechnol. 2019 Sep 2. pii: 10.1038

   Insert

   hAID-XTEN linker-hSpCas9n(D10A)-UGI-NLS-P2A-EGFP
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Oct. 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pdCas9 (GB1079)

   Plasmid

   #75399

   Purpose

   Provides the human codon optimized CDS of Cas9 protein with mutated (D10A, H840A) and inactivated catalytic domains as a level 0 GoldenBraid part for C-terminal fusions

   Depositor

   Diego Orzaez

   Article

   Vazquez-Vilar et al Plant Methods. 2016 Feb 1;12:10. doi: 10

   Insert

   Cas9 coding region with mutated (D10A, H840A) and inactivated catalytic domains (human codon optimised)
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Plant

   Available Since

   Aug. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. AA007

   Plasmid

   #216002

   Purpose

   Fragmid fragment: (Cas protein) canonical Cas9; NGG PAM

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Has Service

   Cloning Grade DNA

   Insert

   Cas9_v1.1 [Sp]
   

   Use

   CRISPR; Fragment

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. AA290

   Plasmid

   #215949

   Purpose

   Fragmid fragment: (guide cassette) guide expression; positive control cell surface

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Has Service

   Cloning Grade DNA

   Insert

   U6_v1; sgCD55_v4; trRNA_v4 [Sp]
   

   Use

   CRISPR; Fragment

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. AA286

   Plasmid

   #215948

   Purpose

   Fragmid fragment: (guide cassette) guide expression; positive control cell surface

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Has Service

   Cloning Grade DNA

   Insert

   U6_v1; sgCD47_v6; trRNA_v4 [Sp]
   

   Use

   CRISPR; Fragment

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. AA427

   Plasmid

   #215961

   Purpose

   Fragmid fragment: (guide cassette) enables iBar UMIs

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Has Service

   Cloning Grade DNA

   Insert

   U6_v1; BsmBI_v0; BsmBI_v5; trRNA_v6 [Sp]
   

   Use

   CRISPR; Fragment

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. PV225

    Plasmid

    #132334

    Purpose

    dCas9 plant transcriptional activator (AT-CBF1) linked constitutive expression cassette for Zea mays

    Depositor

    Joshua Young

    Article

    Young et al Commun Biol. 2019 Oct 18;2:383. doi: 10.

    Insert

    dCas9-AT-CBF1 (CBF1 Cas9 (Streptococcus pyogenes); AT-CBF1 (Arabidopsis thaliana))
    

    Tags

    AT-CBF1

    Expression

    Plant

    Mutation

    Codon optimize for expression and stability in Ze…

    Available Since

    Oct. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEGB2Ω2_SF-35s:hCas9:tNos (GB1103)

    Plasmid

    #75400

    Purpose

    Transcriptional unit for (human codon optimized) Cas9 plant expression driven by the 35S promoter. Specially conceived to be linked with the TU of the kanamycin resistance gene GB1181.

    Depositor

    Diego Orzaez

    Article

    Vazquez-Vilar et al Plant Methods. 2016 Feb 1;12:10. doi: 10

    Insert

    hCas9
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Plant

    Promoter

    35S

    Available Since

    Aug. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. SIN-CMV-Ca9-WPRE

    Plasmid

    #87874

    Purpose

    Transfer plasmid for the production of lentiviral vectors

    Depositor

    Nicole Déglon

    Article

    Merienne et al Cell Rep. 2017 Sep 19;20(12):2980-2991.

    Insert

    spCas9
    

    Use

    Lentiviral

    Tags

    nls

    Mutation

    Human codon-optimized

    Promoter

    CMV

    Available Since

    Sept. 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTBL649 CHYRON2 integration construct

    Plasmid

    #126446

    Purpose

    To integrate the CHYRON2 locus at AAVS1 in human cells.

    Depositor

    Chang Liu

    Article

    Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

    Inserts

    pU6-CHYRON2 hgRNA
    pCMV-puro
    

    Expression

    Mammalian

    Mutation

    The SpCas9 sgRNA constant region is mutated to ma…

    Promoter

    CMV and human U6

    Available Since

    June 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pKMW281_dSpRY

    Plasmid

    #244822

    Purpose

    Mammalian expression of catalytically inactive SpRY Cas9 (dSpRY)

    Depositor

    Jennifer Doudna

    Article

    Shi et al Mol Cell. 2025 May 1;85(9):1730-1742.e9.

    Insert

    dSpRY Cas9
    

    Use

    CRISPR

    Tags

    3xFLAG-SV40 NLS and Nucleoplasmin NLS

    Expression

    Mammalian

    Mutation

    D10A/A61R/H840A/L1111R/D1135L/S1136W/G1218K/E1219…

    Promoter

    CAG

    Available Since

    Sept. 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pKMW121_WT-Cas9_U6-entry

    Plasmid

    #244818

    Purpose

    Mammalian expression of WT Cas9 with Golden Gate-compatible sgRNA spacer cassette

    Depositor

    Jennifer Doudna

    Article

    Shi et al Mol Cell. 2025 May 1;85(9):1730-1742.e9.

    Insert

    SpyCas9 (WT)
    

    Use

    CRISPR

    Tags

    3xFLAG-SV40 NLS and Nucleoplasmin NLS

    Expression

    Mammalian

    Promoter

    CAG, U6

    Available Since

    Sept. 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pKMW493_U6-sgRNA-entry

    Plasmid

    #244823

    Purpose

    Mammalian expression of SpyCas9 single guide RNA with Golden Gate-compatible sgRNA spacer

    Depositor

    Jennifer Doudna

    Article

    Shi et al Mol Cell. 2025 May 1;85(9):1730-1742.e9.

    Insert

    SpyCas9 single guide RNA
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Sept. 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pKMW122_SpG-Cas9_U6-entry

    Plasmid

    #244819

    Purpose

    Mammalian expression of SpG Cas9 with Golden Gate-compatible sgRNA spacer cassette

    Depositor

    Jennifer Doudna

    Article

    Shi et al Mol Cell. 2025 May 1;85(9):1730-1742.e9.

    Insert

    SpG Cas9
    

    Use

    CRISPR

    Tags

    3xFLAG-SV40 NLS and Nucleoplasmin NLS

    Expression

    Mammalian

    Mutation

    D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R

    Promoter

    CAG, U6

    Available Since

    Sept. 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHS484_10xHis_MBP_TEV_dSpRY

    Plasmid

    #244832

    Purpose

    Bacterial expression of catalytically inactive SpRY Cas9 (dSpRY) for protein purification

    Depositor

    Jennifer Doudna

    Article

    Shi et al Mol Cell. 2025 May 1;85(9):1730-1742.e9.

    Insert

    dSpRY Cas9
    

    Use

    CRISPR

    Tags

    10xHis-MBP

    Expression

    Bacterial

    Mutation

    D10A/A61R/H840A/L1111R/D1135L/S1136W/G1218K/E1219…

    Promoter

    T7

    Available Since

    Sept. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pHS71_10xHis_MBP_TEV_WT-Cas9_2xNLS

    Plasmid

    #244833

    Purpose

    Bacterial expression of SpyCas9 (WT) with 2xSV40 nuclear localization sequence for protein purification

    Depositor

    Jennifer Doudna

    Article

    Shi et al Mol Cell. 2025 May 1;85(9):1730-1742.e9.

    Insert

    SpyCas9 (WT)
    

    Use

    CRISPR

    Tags

    10xHis-MBP and 2xSV40 NLS

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pHS23_10xHis_MBP_TEV_SpG-Cas9

    Plasmid

    #244826

    Purpose

    Bacterial expression of SpG Cas9 for protein purification

    Depositor

    Jennifer Doudna

    Article

    Shi et al Mol Cell. 2025 May 1;85(9):1730-1742.e9.

    Insert

    SpG Cas9
    

    Use

    CRISPR

    Tags

    10xHis-MBP

    Expression

    Bacterial

    Mutation

    D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R

    Promoter

    T7

    Available Since

    Sept. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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