178,769 results
-
Plasmid#71446PurposeRetroviral expression of S727A-STAT3. Please note that this plasmid contains S727A-STAT3 tagged with FLAG, not GFP.DepositorAvailable sinceNov. 30, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by
-
pGZ108 (pFA6a-3FLAG-MNase-kanMX6)
Plasmid#70231PurposeChEC-tagging vector encoding a short linker and aa 83-231 of Mnase in pFA6a-3HA-kanMX6 replacing the 3xHA tag. These vectors retain compatibility with the F2/R1 primer pairs commonly used for epitopeDepositorInsert3xFLAG tag and aa 83-231 of Mnase
UseYeast genomic targetingAvailable sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pY011 (pcDNA3.1-hLb2Cpf1)
Plasmid#69983PurposeExpresses humanized Lb2Cpf1DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserthLb2Cpf1
UseCRISPRTagsNLS-3xHAExpressionMammalianPromoterCMVAvailable sinceOct. 8, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
GRM2-Tango
Plasmid#66388PurposeExpression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocationDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertGRM2 (GRM2 Human)
TagsFLAGExpressionMammalianPromoterCMVAvailable sinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
CXCR7-Tango
Plasmid#66265PurposeExpression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocationDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCXCR7 (ACKR3 Human)
TagsFLAGExpressionMammalianPromoterCMVAvailable sinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Cas9/pTREX-n
Plasmid#68708PurposeExpresses the fusion gene CAS9-HA-2xNLS-GFP in the pTREX-n backbone. This vector is used for cloning a specific sgRNA by BamHI, to be co-expressed with Cas9 for genome editing in Trypanosoma cruzi.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertFusion gene Cas9-HA-2xNLS-GFP from vector pMJ920 (Addgene) .
UseCRISPRTagsFusion gene Cas9-HA-2xNLS-GFP from vector pMJ920 …MutationC4239T mutation that eliminates a BamHI restricti…Available sinceSept. 14, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMJH46
Plasmid#67272PurposeConjugally transferable red recombinogenic plasmidDepositorInserttraJ, cat, oriT
UseRed recombinasePromotercat gene promoter from PGNS-BACAvailable sinceSept. 3, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Prig-3::CD4::spGFP1-10
Plasmid#65829PurposeFor membrane contactDepositorPublicationInsertHomo sapiens CD4 molecule (CD4), transcript variant 2, mRNA (CD4 Human)
TagsspGFP1-10ExpressionWormPromoterPrig-3Available sinceAug. 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRC_RR_VP1_r1c3
Plasmid#65724PurposeRescued infectious variant from pAAV2_RaPID library; mCherry insertion in place of the deleted amino acid sequence 453-GTTTQSRDepositorInsertmCherry
UseAAVAvailable sinceAug. 17, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
ERoxBFP
Plasmid#68126Purposemammalian expression of ER localized oxBFPDepositorInsertoxBFP
TagsKDEL ER retention sequence and bovine prolactin s…ExpressionMammalianPromoterCMVAvailable sinceAug. 10, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pYLsgRNA-OsU6a/LacZ
Plasmid#66195Purposecloning of sgRNAs for expression in plantsDepositorTypeEmpty backboneUseCloning vectorPromoterOsU6aAvailable sinceAug. 10, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pOPINS-UBE3C
Plasmid#66711PurposeExpresses UBE3C HECT domain in E. coliDepositorAvailable sinceJuly 17, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pUC18R6K-mini-Tn7T
Plasmid#64958Purposemini-Tn7 base vector with transcriptional terminatorDepositorPublicationTypeEmpty backboneExpressionBacterialAvailable sinceJuly 6, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBa-KIF5C 559-tdTomato-FKBP
Plasmid#64211PurposeKIF5C motor protein aa1-559 with C-terminal tandem dimer Tomato and FKBP tagsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertKif5c (Kif5c Rat)
TagsFKBP and tdTomatoExpressionMammalianMutationmotor domain aa1-559Promoterchicken Beta ActinAvailable sinceJune 17, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3-SEP-Glur2 (MCA10)
Plasmid#64941Purposemammalian expression of rat GluR2DepositorAvailable sinceJune 16, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLPCX-IRES-GFP
Plasmid#65436PurposeFor transfection or retroviral production. Mammalian expression of EGFP driven by the ECMV IRES.DepositorInsertIRES-GFP
UseRetroviralExpressionMammalianPromoterCMVAvailable sinceJune 11, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLPCX-Cx43-IRES-GFP
Plasmid#65433PurposeMammalian expression of Cx43 and EGFP seperately driven by the ECMV IRES. For transfection or retroviral productionDepositorAvailable sinceJune 2, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLVX-Tight-Puro_Sox10
Plasmid#46536PurposeA lentiviral vector for the expression of mouse Sox10 under doxycyline control.DepositorAvailable sinceMay 28, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
GFP-BRD1
Plasmid#65375PurposeGFP-tagged BRD protein, which can be used to be expressed in mammalian cells.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertBRD1 (BRD1 Human)
TagsEmGFP and V5ExpressionMammalianMutationnonePromoterCMVAvailable sinceMay 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGGDestTol2LC-3sgRNA
Plasmid#64241Purposedestination vector for three U6x:sgRNA cassettesDepositorTypeEmpty backboneUseCRISPRAvailable sinceMay 5, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pGGDestTol2LC-2sgRNA
Plasmid#64240Purposedestination vector for two U6x:sgRNA cassettesDepositorTypeEmpty backboneUseCRISPRAvailable sinceMay 4, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Tet-pLKO-puro-SOX2
Plasmid#47540PurposeTet inducible knockdown of SOX2.DepositorInsertSOX2 shRNA
UseLentiviral and RNAiExpressionMammalianPromoterH1Available sinceApril 28, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pQHA-USP7 WT puroR
Plasmid#46753PurposeRetroviral vector that expresses wild type HA-tagged human USP7DepositorAvailable sinceApril 2, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMOS001: CMV-Optopatch2_FCK
Plasmid#62984PurposeCoexpression plasmid of QuasAr2 and CheRiff for all-optical electrophysiology under CMV promoterDepositorInsertOptopatch2
UseLentiviralExpressionMammalianPromoterCMVAvailable sinceMarch 12, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
mDsRed-Golgi-7
Plasmid#55832PurposeLocalization: Golgi, Excitation: 557, Emission: 585DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertGolgi (B4GALT1 Human)
TagsmDsRedExpressionMammalianMutationaa 1-82 of NM_001497.3PromoterCMVAvailable sinceMarch 12, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Spectrin-C-cpstFRET
Plasmid#61111PurposeExpression of Spectrin-C-term cpstFRET to measure mechanical stress and report multidimensional stress fieldsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertspectrin-cpstFRET
ExpressionMammalianPromoterCMVAvailable sinceMarch 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P2-MDM2 (17-125)
Plasmid#62063PurposeExpression of human MDM2 (17-125) in e. coliDepositorInsertMDM2 (MDM2 Human)
TagsGSTExpressionBacterialMutationcontains residues 17-125PromotertacAvailable sinceFeb. 23, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSBtet-RH
Plasmid#60500PurposeSB-transposon with inducible SfiI cloning site for GOI (contains firefly luciferase) and constitutive expression of RFP, rtTA and hygromycin resistance geneDepositorTypeEmpty backboneUseTransposonExpressionMammalianPromoterTCE & RPBSAAvailable sinceFeb. 11, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSBtet-RB
Plasmid#60506PurposeSB-transposon with inducible SfiI cloning site for GOI (contains firefly luciferase) and constitutive expression of RFP, rtTA and blasticidin resistance geneDepositorTypeEmpty backboneUseTransposonExpressionMammalianPromoterTCE & RPBSAAvailable sinceFeb. 11, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
single polypeptide FPX biosensor for caspase-1
Plasmid#60885PurposeFPX biosensor for caspase-1DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsingle polypeptide FPX biosensor for caspase-1
TagsHindIII site and NES sequence LALKLAGLDIGSExpressionMammalianPromoterCMVAvailable sinceJan. 28, 2015AvailabilityAcademic Institutions and Nonprofits only