We narrowed to 8,677 results for: sgrna

1. pLenti-Blast-sgNT

   Plasmid

   #138678

   Purpose

   Expresses a Non-targeting sgRNA and Cas9

   Depositor

   Reuben Shaw

   Article

   Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

   Insert

   sgNT
   

   Use

   Lentiviral

   Promoter

   hU6

   Available Since

   Feb. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMonAID_nCas9-PmCDA_Hyg_ALS

   Plasmid

   #91693

   Purpose

   Monocot Target-AID vector expressing rice-optimized nCas9-PmCDA1 with sgRNA targeting OsAls

   Depositor

   Akihiko Kondo

   Article

   Shimatani et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

   Insert

   SpCas9
   

   Tags

   PmCDA1

   Expression

   Plant

   Mutation

   D10A for nickase Cas9

   Promoter

   2x35S

   Available Since

   June 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pPBbsr2-Gnaq

   Plasmid

   #170298

   Purpose

   Encoding mouse Gnaq

   Depositor

   Michiyuki Matsuda

   Article

   Konishi et al Cancer Res. 2021 May 25. pii: 0008-5472.

   Insert

   A recombinant mouse Gnaq (Gnaq Mouse)
   

   Tags

   N/A

   Expression

   Mammalian

   Mutation

   Asilent mutation was introduced into the sgRNA-ta…

   Available Since

   July 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLenti-Puro-sgSNRK

   Plasmid

   #138694

   Purpose

   Expresses a human SNRK-targeting sgRNA and Cas9

   Depositor

   Reuben Shaw

   Article

   Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

   Insert

   sgSNRK human (SNRK Human)
   

   Use

   Lentiviral

   Promoter

   hU6

   Available Since

   Feb. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. sgCTNNB1 Nicking

   Plasmid

   #169845

   Purpose

   Express a nicking sgRNA used for installation of the oncogenic S45F or S45del in Ctnnb1 in mouse liver.

   Depositor

   Wen Xue

   Article

   Liu et al Nat Commun. 2021 Apr 9;12(1):2121. doi:

   Insert

   sgCTNNB1 Nicking (Ctnnb1 Mouse)
   

   Expression

   Mammalian

   Available Since

   June 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJMP1333

   Plasmid

   #119268

   Purpose

   rfp "test" strain

   Depositor

   Carol Gross, Jason Peters, Oren Rosenberg

   Article

   Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

   Insert

   sgRNA NT1/RR1 (rfp)
   

   Expression

   Bacterial

   Mutation

   D10A and H840A

   Available Since

   Jan. 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. qTAG-N-Blast-3xHA-LMNB1

   Plasmid

   #207778

   Purpose

   Donor template for Blast-2A-3xHA insertion into the N-terminus of the LMNB1 locus. To be co-transfected with sgRNA plasmid px330-PITCh-LMNB1 Addgene #207770

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   LMNB1 Homology Arms flanking a Blast-3xHA Cassette (LMNB1 Human)
   

   Use

   CRISPR; Donor template

   Expression

   Mammalian

   Available Since

   Nov. 29, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. qTAG-N-Puro-sTagRFP-TUBA1B

   Plasmid

   #207769

   Purpose

   Donor template for Puro-2A-sTagRFP insertion into the N-terminus of the TUBA1B locus for tubulin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-TUBA1B Addgene #207763

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   TUBA1B Homology Arms flanking a Puro-sTagRFP Cassette (TUBA1B Human)
   

   Use

   CRISPR; Donor template

   Expression

   Mammalian

   Available Since

   Nov. 15, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pU6-(BsaI)_CBh-Cas9-T2A-mCherry

   Plasmid

   #135012

   Purpose

   Expression vector for sgRNAs cloned into the BsaI sites and for expression of Cas9 linked to mCherry via a T2A peptide

   Depositor

   Yosef Shaul

   Article

   Reuven et al Biomolecules. 2019 Oct 8;9(10). pii: bio

   Insert

   humanized S. pyogenes Cas9
   

   Tags

   3x Flag, 3xFLAG (N terminal on insert), NLS, and …

   Expression

   Mammalian

   Promoter

   CBh

   Available Since

   Jan. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pODN-mNG-RHOA

    Plasmid

    #183836

    Purpose

    Repair template for the N-terminal tagging of RhoA with mNeonGreen in human cells using CRISPR/Cas9.

    Depositor

    Alisa Piekny

    Article

    Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

    Insert

    RHOA homology arms with mNeonGreen-linker (RHOA Human)
    

    Use

    CRISPR; Donor template

    Tags

    mNeonGreen-linker

    Expression

    Mammalian

    Mutation

    Homology arms contain point mutations to remove t…

    Available Since

    May 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. eSpCas9-EF-5Nanog_2

    Plasmid

    #174871

    Purpose

    CRISPR vector for generating Nanog STREAMING-tag KI cell

    Depositor

    Hiroshi Ochiai

    Article

    Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

    Insert

    sgRNA for mouse Nanog (Nanog Synthetic)
    

    Use

    CRISPR

    Available Since

    Dec. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. Cas12f-GE ver4.0

    Plasmid

    #176543

    Purpose

    Expresses Cas12f-GE in mammalian cells

    Depositor

    Yong-Sam Kim

    Article

    Kim et al Nat Biotechnol. 2021 Sep 2. pii: 10.1038

    Inserts

    Cas12f-GE ver4.0
    Cas12f-GE ver4.0
    

    Expression

    Mammalian

    Promoter

    U6 and chicken β-actin

    Available Since

    Nov. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLentiCRISPRv2 sgBLM10

    Plasmid

    #127643

    Purpose

    Knock-out of human BLM

    Depositor

    Nicolas Manel

    Article

    Gratia et al J Exp Med. 2019 May 6;216(5):1199-1213.

    Insert

    IRF3 sgRNA (IRF3 Human)
    

    Use

    Lentiviral

    Available Since

    July 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDicAID_nCas9-PmCDA-2A-NptII_ETR

    Plasmid

    #91695

    Purpose

    Dicot Target-AID vector expressing dicot-optimized nCas9-PmCDA1-2A-NptII with sgRNA targeting SlEtr

    Depositor

    Akihiko Kondo

    Article

    Shimatani et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

    Insert

    SpCas9
    

    Tags

    PmCDA1

    Expression

    Plant

    Mutation

    D10A for nickase Cas9

    Promoter

    PcUbi

    Available Since

    June 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. qTAG-C-Solo-mStayGold-CLTC

    Plasmid

    #227313

    Purpose

    Donor template for mStayGold insertion into the C-terminus of the CLTC locus. For clathrin heavy chain visualization. To be co-transfected with sgRNA px330-PITCh-CLTC (Addgene #227312)

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    CLTC Homology Arms flanking a mStayGold Tag (CLTC Human)
    

    Use

    CRISPR; Donor template

    Expression

    Mammalian

    Promoter

    Promoterless

    Available Since

    Nov. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pU6-sgGAL4-1

    Plasmid

    #46915

    Purpose

    Human pSico-based U6 vector containing murine U6 promoter and sgRNA targeting GAL4 UAS promoter

    Depositor

    Stanley Qi, Jonathan Weissman

    Article

    Gilbert et al Cell. 2013 Jul 9. pii: S0092-8674(13)008

    Inserts

    sgGAL4-1
    Puromycin resistance and mCherry
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    CMV and U6

    Available Since

    Oct. 1, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. SPDK3889(TRV-RNA2-pPEBV-MCS-AtMet-tRNA)

    Plasmid

    #149277

    Purpose

    Modified Tobacco rattle virus RNA2 vector that could be used for expression of sgRNAs

    Depositor

    Savithramma Dinesh-Kumar

    Article

    Ellison et al Nat Plants. 2020 Jun 1. pii: 10.1038/s41

    Insert

    Modified TRV RNA2 with PEBV promoter and AtMet-tRNA mobile RNA sequence
    

    Use

    T-dna vector

    Mutation

    T2318C (DNA) in PEBV coat protein sequence

    Available Since

    June 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. Cas12f-GE ver3.0

    Plasmid

    #176508

    Purpose

    Expresses Cas12f-GE in mammalian cells

    Depositor

    Yong-Sam Kim

    Article

    Kim et al Nat Biotechnol. 2021 Sep 2. pii: 10.1038

    Inserts

    Cas12f-GE ver3.0
    Cas12f-GE ver3.0
    

    Expression

    Mammalian

    Promoter

    U6 and chicken β-actin

    Available Since

    Oct. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pODN-mR2-ACTB

    Plasmid

    #183868

    Purpose

    Repair template for the N-terminal tagging of beta-actin with mRuby2 in human cells using CRISPR/Cas9.

    Depositor

    Alisa Piekny

    Article

    Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

    Insert

    ACTB homology arms with mRuby2-linker (ACTB Human)
    

    Use

    CRISPR; Donor template

    Tags

    mRuby2-linker

    Expression

    Mammalian

    Mutation

    Homology arms contain point mutations to remove t…

    Available Since

    May 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. plenti-px330-CRBN-T2-pGK-Pur

    Plasmid

    #107383

    Purpose

    Mammalian expression CRISPR/Cas9

    Depositor

    William Kaelin

    Article

    Lu et al Science. 2014 Jan 17;343(6168):305-9. do

    Insert

    sgRNA targeting CRBN (CRBN Human)
    

    Use

    Lentiviral; Crispr

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    June 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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