We narrowed to 17,814 results for: She

1. pMMLV-EGFP-P2A-Puro

   Plasmid

   #160226

   Purpose

   Expresses EGFP in mammalian cells

   Depositor

   Jason Sheltzer

   Article

   Lukow et al Dev Cell. 2021 Sep 13;56(17):2427-2439.e

   Insert

   EGFP-P2A-PuroR
   

   Use

   Retroviral

   Expression

   Bacterial and Mammalian

   Promoter

   LTR

   Available Since

   Sept. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLenti_rtTA-P2A-BFP_HygroR

   Plasmid

   #186738

   Purpose

   rtTA-P2A-BFP expression construct cloned into lentiviral backbone

   Depositor

   Richard Sherwood

   Article

   Hamilton et al bioRxiv. 2023 Jan 10:2023.01.09.500804.

   Insert

   rtTA
   

   Use

   Lentiviral

   Tags

   P2A-BFP

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   April 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. His-FGE

   Plasmid

   #75147

   Purpose

   Expression vector for His(6) tagged Formylglycine Generating Enzyme (FGE) in bacterial host.

   Depositor

   Richard Fishel

   Article

   Liu et al Sci Rep. 2015 Nov 19;5:16883. doi: 10.10

   Insert

   FGE
   

   Tags

   Histidine tag and Thrombin

   Expression

   Bacterial

   Mutation

   N/A

   Promoter

   T7

   Available Since

   July 8, 2016

   Availability

   Academic Institutions and Nonprofits only

4. PB-3-FT-ENGRAM

   Plasmid

   #179158

   Purpose

   piggyBac plasmid for 3'-FT ENGRAM recorder

   Depositor

   Jay Shendure

   Article

   Chen et al Nature. 2024 Aug;632(8027):1073-1081. do

   Insert

   Csy4
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Mammalian

   Promoter

   minP

   Available Since

   Dec. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pProEx-IDE-wt

   Plasmid

   #99014

   Purpose

   Express human Insulin Degrading Enzyme (IDE) in E. coli.

   Depositor

   Wei-Jen Tang

   Article

   Shen et al Nature. 2006 Oct 19;443(7113):870-4. Epu

   Insert

   insulin degrading enzyme (IDE Human)
   

   Tags

   His tag

   Expression

   Bacterial

   Promoter

   TRC

   Available Since

   Aug. 15, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pPbHiT_3xmyc_hsp70UTR_hdhfr/yfcu

   Plasmid

   #216421

   Purpose

   Empty backbone to express gene specific gRNA and carry the homology repair template for CRISPR editing of Plasmodium berghei using the PbHiT system.

   Depositor

   Ellen Bushell

   Article

   Jonsdottir et al bioRxiv 2024.04.20.590404

   Type

   Empty backbone

   Use

   CRISPR; Expression in plasmodium berghei

   Tags

   3xmyc

   Expression

   Bacterial

   Available Since

   April 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pPbU6_hdhfr/yfcu

   Plasmid

   #216422

   Purpose

   Empty backbone to express gene specific gRNA from the Plasmodium berghei U6 promoter for traditional CRISPR editing.

   Depositor

   Ellen Bushell

   Article

   Jonsdottir et al bioRxiv 2024.04.20.590404

   Type

   Empty backbone

   Use

   CRISPR; Expression in plasmodium berghei

   Expression

   Bacterial

   Available Since

   April 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. trPAGmEos3.2

   Plasmid

   #203752

   Purpose

   Encodes the transmembrane domain from PAG/CSK, including its 2 palmitoylation sites, with mEos3.2 fused on the C terminus. Used as a fluorescent plasma membrane probe.

   Depositor

   Sarah Veatch

   Article

   Shelby et al Nat Chem Biol. 2023 Jun;19(6):750-758. d

   Insert

   trPAG (PAG1 Human)
   

   Tags

   mEos3.2

   Expression

   Mammalian

   Available Since

   Aug. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. piGECI-N1

   Plasmid

   #160421

   Purpose

   Cytoplasmic expression of a near-infrared genetically encoded calcium indicator iGECI

   Depositor

   Vladislav Verkhusha

   Article

   Shemetov et al Nat Biotechnol. 2021 Mar;39(3):368-377.

   Insert

   iGECI
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. HBT-pcoCas9

    Plasmid

    #52254

    Purpose

    transient expression of pcoCas9 gene in plant cells

    Depositor

    Jen Sheen

    Article

    Li et al Nat Biotechnol. 2013 Aug;31(8):688-91. d

    Insert

    pcoCas9
    

    Use

    CRISPR; Plant expression

    Tags

    FLAG and NLS

    Promoter

    hybrid constitutive promoter 35SPPDK

    Available Since

    March 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBITE-EGFRvIII-F265-OKT3

    Plasmid

    #190679

    Purpose

    Encodes an EGFRvIII-CD3 BITE protein (F265 clone)

    Depositor

    Scott McComb

    Article

    Shepherd et al PLoS One. 2023 Jun 22;18(6):e0273884. do

    Insert

    Anti-EGFRvIII-scFv-F265-modularized-OKT3-HisTag
    

    Use

    Lentiviral

    Tags

    mNeonGreen

    Expression

    Mammalian

    Promoter

    EFS

    Available Since

    Feb. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. MC177VSG221RNAi

    Plasmid

    #59730

    Purpose

    For use in trypanosome cell lines already expressing T7 polymerase and Tet-repressor. Tetracycline induction results in the production of dsRNA targeting VSG221.

    Depositor

    Gloria Rudenko

    Article

    Sheader et al Proc Natl Acad Sci U S A. 2005 Jun 14;10

    Insert

    VSG221 RNAi fragment
    

    Use

    RNAi

    Promoter

    opposing T7 promoters

    Available Since

    Aug. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. piggyFlex

    Plasmid

    #218234

    Purpose

    A piggyBac transposon-based gRNA expression vector, to allow for genomic integration and stable expression of gRNAs. Contains both antibiotic (puromycin) and fluorophore (GFP) markers.

    Depositor

    Jay Shendure

    Article

    Chardon et al Nat Commun. 2024 Sep 18;15(1):8209. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Available Since

    May 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pST1374-N-NLS-flag-linker-Cas9-H840A

    Plasmid

    #51129

    Purpose

    Cas9 nickase optimized for nuclear import.

    Depositor

    Xingxu Huang

    Article

    Shen et al Nat Methods. 2014 Mar 2. doi: 10.1038/nm

    Insert

    Cas9 nickase
    

    Use

    CRISPR

    Tags

    Flag and NLS

    Expression

    Mammalian

    Mutation

    H840A

    Promoter

    CMV

    Available Since

    March 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMMLV-mRFP1-P2A-Puro

    Plasmid

    #160228

    Purpose

    Expresses mRFP in mammalian cells

    Depositor

    Jason Sheltzer

    Article

    Lukow et al Dev Cell. 2021 Sep 13;56(17):2427-2439.e

    Insert

    mRFP1-P2A-Puro
    

    Use

    Retroviral

    Expression

    Bacterial and Mammalian

    Promoter

    LTR

    Available Since

    Sept. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pJL1-sfGFP-DQNAT

    Plasmid

    #198210

    Purpose

    pJL1 plasmid encoding superfolder GFP modified at the C-terminus with 30 amino acids containing an optimal DQNAT sequon followed by a 6x-His tag

    Depositor

    Michael Jewett

    Article

    Hershewe et al Nat Commun. 2021 Apr 22;12(1):2363. doi:

    Insert

    sfGFP_DQNAT
    

    Tags

    6x His Tag and DQNAT glycosylation tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. TfR-pHoran4

    Plasmid

    #61547

    Purpose

    Human transferrin receptor tagged with pH sensitive orange/red fluorescent protein pHoran4

    Depositor

    David Perrais

    Article

    Shen et al J Cell Biol. 2014 Nov 10;207(3):419-32.

    Insert

    Transferrin receptor (TFRC Human)
    

    Tags

    pHoran4

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 19, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGL3-24p3/LCN2-Luc

    Plasmid

    #25463

    Depositor

    Sarah Gaffen

    Article

    Shen et al J Leukoc Biol. 2005 Mar . 77(3):388-99.

    Insert

    24p3/lipocalin 2 proximal promoter (Lcn2 Mouse)
    

    Use

    Luciferase

    Mutation

    none

    Available Since

    June 25, 2010

    Availability

    Academic Institutions and Nonprofits only

19. pU6_(GLuc)_INT[3xMS2_SL]

    Plasmid

    #68426

    Purpose

    Transient expression in mammalian cells of an "INT" construct_bearing three MS2 Stem-loops, targeting the GLuc reporter. U6 promoter

    Depositor

    John Rinn

    Article

    Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

    Insert

    INT construct_bearing three MS2 Stem-loops
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Mammalian

    Promoter

    human U6

    Available Since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pRc/CMV2 Dvl-2-Myc

    Plasmid

    #42194

    Depositor

    Robert Lefkowitz, Shin-ichi Yanagawa

    Article

    Lee et al J Biol Chem. 1999 Jul 23;274(30):21464-7

    Insert

    Dvl 2 (Dvl2 Mouse)
    

    Tags

    Myc

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 5, 2013

    Availability

    Academic Institutions and Nonprofits only

21. pET-28a-His6-SUMO-Munc18-1wt

    Plasmid

    #135550

    Purpose

    Express rat Munc18-1 in E. coli BL21, purified via HisPur Ni-NTA Resin

    Depositor

    Jingshi Shen

    Article

    Yu et al Methods Mol Biol. 2019;1860:237-249. doi

    Insert

    Munc18-1wt
    

    Tags

    6xHis tag, thrombin recognition and cleavage site…

    Expression

    Bacterial

    Mutation

    No ATG start codon

    Promoter

    T7 promoter

    Available Since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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22. pMMLV-EBFP-P2A-Puro

    Plasmid

    #160227

    Purpose

    Expresses EBFP in mammalian cells

    Depositor

    Jason Sheltzer

    Article

    Lukow et al Dev Cell. 2021 Sep 13;56(17):2427-2439.e

    Insert

    EBFP-P2A-Puro
    

    Use

    Retroviral

    Expression

    Bacterial and Mammalian

    Promoter

    LTR

    Available Since

    Sept. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. EGR-1 TurboRFP

    Plasmid

    #52306

    Purpose

    Fluid Shear Stress inducible Red Fluorescent Protein for mammalian expression

    Depositor

    Joel Voldman

    Article

    Varma et al Lab Chip. 2015 Feb 4.

    Insert

    EGR-1 Promoter (Egr1 Mouse)
    

    Tags

    TurboRFP

    Expression

    Mammalian

    Available Since

    Feb. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pTXB1-EXO1b

    Plasmid

    #68267

    Purpose

    Bacterial expression of human EXO1

    Depositor

    Stefano Ferrari

    Article

    El-Shemerly et al Cancer Res. 2005 May 1;65(9):3604-9.

    Insert

    EXO1 (EXO1 Human)
    

    Tags

    Mxe intein/chitin binding domain

    Expression

    Bacterial

    Mutation

    b form, codons 1-846. Please see depositor commen…

    Promoter

    T7

    Available Since

    Aug. 12, 2015

    Availability

    Academic Institutions and Nonprofits only

25. pBITE-EGFRvIII-F271-OKT3

    Plasmid

    #190682

    Purpose

    Encodes an EGFRvIII-CD3 BITE protein (F271 clone)

    Depositor

    Scott McComb

    Article

    Shepherd et al PLoS One. 2023 Jun 22;18(6):e0273884. do

    Insert

    Anti-EGFRvIII-scFv-F271-modularized-OKT3-HisTag
    

    Use

    Lentiviral

    Tags

    mNeonGreen

    Expression

    Mammalian

    Promoter

    EFS

    Available Since

    Feb. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pJL1-sfGFP-AQNAT

    Plasmid

    #198211

    Purpose

    pJL1 plasmid encoding superfolder GFP modified at the C-terminus with 30 amino acids containing a non-permissible AQNAT sequon followed by a 6x-His tag

    Depositor

    Michael Jewett

    Article

    Hershewe et al Nat Commun. 2021 Apr 22;12(1):2363. doi:

    Insert

    sfGFP_AQNAT
    

    Tags

    6x His Tag and AQNAT mutated (non-permissible) gl…

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pJL1-sfGFP-MOOR

    Plasmid

    #198212

    Purpose

    pJL1 plasmid encoding superfolder GFP modified at the C-terminus with 32 amino acids containing the minimum optimal O-linked recognition site (MOOR) followed by a 6x-His tag

    Depositor

    Michael Jewett

    Article

    Hershewe et al Nat Commun. 2021 Apr 22;12(1):2363. doi:

    Insert

    sfGFP_MOOR
    

    Tags

    6x His Tag and MOOR glycosylation tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pKT-iRFP-KAN

    Plasmid

    #64687

    Purpose

    pKT vector encoding yeast-codon optimized infrared fluorescent protein iRFP.

    Depositor

    Erin O'Shea

    Article

    Hansen et al Mol Syst Biol. 2013 Nov 5;9:704. doi: 10

    Insert

    iRFP
    

    Expression

    Yeast

    Mutation

    Nucleotide sequence has been yeast-codon optimize…

    Available Since

    May 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pXJ-His-VTCN1

    Plasmid

    #232664

    Purpose

    transient expresses VTCN1 in mammalian cells

    Depositor

    Chen-Yang Shen

    Article

    Chou et al Cell Rep. 2024 Jan 23;43(1):113641. doi:

    Insert

    VTCN1 (VTCN1 Human)
    

    Tags

    His and His (retained after signal peptide cleava…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. CD200RCD4d3+4-bio-linker-His

    Plasmid

    #36153

    Purpose

    A positive control receptor bait with bio-linker-His (rat CD4d3+4-bio-linker-his) and prey (rat CD200 receptor) EXPRESs plasmid

    Depositor

    Gavin Wright

    Article

    Bushell et al Genome Res. 2008 Apr;18(4):622-30. Epub

    Insert

    CD200R (Cd200r1 Rat)
    

    Tags

    His tag, biotinylation peptide, and ratCD4d3+4

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 14, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. p194-RV-CAG-dio-mScarlet-T2A-Brn2

    Plasmid

    #204741

    Purpose

    Conditional expression of target gene Brn2 with fluorescent reporter mScarlet

    Depositor

    Lynette Lim

    Article

    Fisher et al Neuron. 2023 Dec 7:S0896-6273(23)00887-5

    Insert

    Brn2
    

    Use

    Retroviral

    Promoter

    CAG

    Available Since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pMXs-Sig10-Fc-IG

    Plasmid

    #183722

    Purpose

    Expresses the fusion protein containing the extracellular part of human Siglec-10 and the Fc region of human IgG1, and GFP

    Depositor

    Takeshi Tsubata

    Article

    Alborzian Deh Sheikh et al J Autoimmun. 2021 Jan;116:102571. doi: 1

    Insert

    Siglec-10
    

    Use

    Retroviral

    Tags

    Fc of human IgG1

    Available Since

    June 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pXJ-Myc-FOXA1

    Plasmid

    #232645

    Purpose

    transient expresses FOXA1 in mammalian cells

    Depositor

    Chen-Yang Shen

    Article

    Chou et al NPJ Genom Med. 2022 Jan 19;7(1):4. doi:

    Insert

    FOXA1 (FOXA1 Human)
    

    Tags

    Myc

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pCMV-BiP-Myc-KDEL P495L

    Plasmid

    #27166

    Depositor

    Ron Prywes

    Article

    Shen et al Dev Cell. 2002 Jul . 3(1):99-111.

    Insert

    BiP (HSPA5 Human)
    

    Tags

    KDEL and Myc

    Expression

    Mammalian

    Mutation

    Peptide Binding Mutant: P495L

    Available Since

    Jan. 25, 2011

    Availability

    Academic Institutions and Nonprofits only

35. pCMV-BiP-Myc-KDEL T37G

    Plasmid

    #27165

    Depositor

    Ron Prywes

    Article

    Shen et al Dev Cell. 2002 Jul . 3(1):99-111.

    Insert

    BiP (HSPA5 Human)
    

    Tags

    KDEL and Myc

    Expression

    Mammalian

    Mutation

    ATP bound form: T37G

    Available Since

    Jan. 25, 2011

    Availability

    Academic Institutions and Nonprofits only

36. pU6_(GLuc)_INT[BoBS]

    Plasmid

    #68431

    Purpose

    Transient expression in mammalian cells of an "INT" construct_bearing the "Bunch of Baby Spinach" (or "BoBS") construct, targeting the GLuc reporter. U6 promoter

    Depositor

    John Rinn

    Article

    Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

    Insert

    INT construct_bearing the "Bunch of Baby Spinach" (or "BoBS") construct
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Mammalian

    Promoter

    human U6

    Available Since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pAGH42

    Plasmid

    #107249

    Purpose

    Vipp1-mGFPmut3 (or vipp1-gfp) construct with spectinomycin resistance cassette downstream used to replace the native copy of Vipp1 in Synechocystis (sll0617).

    Depositor

    Erin O'Shea

    Article

    Gutu et al Mol Microbiol. 2018 Jan 22. doi: 10.1111

    Insert

    vipp1-gfp
    

    Use

    Suicide vector for destined for double homologous…

    Tags

    mGFPmut3

    Expression

    Bacterial

    Promoter

    native promoter of sll0617

    Available Since

    April 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pET30a-FluA-NP

    Plasmid

    #165092

    Purpose

    protein expression in E. coli

    Depositor

    Rick Tarleton

    Article

    Shen et al medRxiv [Preprint]. 2021 Jan 27:2021.01.

    Insert

    Influenza A virus(A/Georgia/03/2019(H1N1) nucleocapsid protein
    

    Expression

    Bacterial

    Available Since

    Feb. 11, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

39. HOXA9 (murine) FLAG MSCV

    Plasmid

    #8515

    Depositor

    Corey Largman

    Article

    Shen et al Mol Cell Biol 1997 Nov;17(11):6448-58.

    Insert

    HOXA9 (Hoxa9 Mouse)
    

    Use

    Retroviral

    Tags

    FLAG (DYKDDDDK) and IRES-GFP

    Expression

    Mammalian

    Mutation

    makes GFP from an IRES driving translation from a…

    Available Since

    May 30, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pU6_(GLuc)_INT[3xPP7_SL]

    Plasmid

    #68424

    Purpose

    Transient expression of an "INT" construct_bearing three PP7 Stem-loops, targeting the GLuc reporter, in mammalian cells. U6 promoter

    Depositor

    John Rinn

    Article

    Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

    Insert

    INT construct bearing three PP7 Stem-loops
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Mammalian

    Promoter

    human U6

    Available Since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge