We narrowed to 4,347 results for: ara-2
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Plasmid#186401PurposeDestination vector for ascidian transgenesis by electroporation of a Gateway-cloned fusion of a gene of interest with a C-ter Venus under control of the pFOG ectodermal regulatory sequence.DepositorTypeEmpty backboneUseGateway destination vectorTagsExpressionMutationPromoterAvailable sinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only
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pDEST-pSP172BSSPE-pFOGc::venus-RfA
Plasmid#186400PurposeDestination vector for ascidian transgenesis by electroporation of a Gateway-cloned fusion of N-ter Venus and a gene of interest under control of the pFOG ectodermal regulatory sequence.DepositorTypeEmpty backboneUseGateway destination vectorTagsExpressionMutationPromoterAvailable sinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST-pSP172BSSPE-Swa1::venus-RfA
Plasmid#186398PurposeDestination vector for ascidian transgenesis by electroporation of a Gateway-cloned fusion of N-ter Venus and a gene of interest under control of a reg. sequence in the Swa1 restriction site.DepositorTypeEmpty backboneUseGateway destination vectorTagsExpressionMutationPromoterAvailable sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST-pSP172BSSPE-Swa1::RfA-HA
Plasmid#186405PurposeDestination vector for ascidian transgenesis by electroporation of a Gateway-cloned fusion of a gene of interest with a C-ter HA tag under control of a reg. sequence in the Swa1 restriction site.DepositorTypeEmpty backboneUseGateway destination vectorTagsExpressionMutationPromoterAvailable sinceAug. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFX-GalT-Sirius
Plasmid#174469PurposeExpress Sirius with golgi apparatus-targeting sequence in mammalian cellsDepositorInsertSirius with golgi apparatus-targeting sequence
UseTagsβ1,4-galactose transferaseExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCS2-zfCSF3b
Plasmid#168202PurposeExpress zebrafish Csf3b, mRNA synthesisDepositorInsertCsf3b (csf3b Zebrafish)
UseVertebrate expression (zebrafish, chicken, xenopu…TagsExpressionMammalianMutationPromoterAvailable sinceJan. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBz173_CsoS2_N2_GFP
Plasmid#140853PurposeHis-tagged second N-peptide of NTD fused to Superfolder GFP ([N2]-GFP) used for BLI, pET-14b backboneDepositorInsertCsoS2 NTD repeat 2 fused to GFP
UseTagsHexahistidine tag, Strep-Tag II, and Superfolder …ExpressionBacterialMutationPromoterT7Available sinceMay 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pVH009
Plasmid#80399PurposeControl plasmid with no anti-scaffold feedback. Contains pBAD-CusR (response regulator) and pCusR-YFP-AAV.DepositorUseSynthetic BiologyTagsC-terminal LZx domain and N-terminal 3xFLAG tag, …ExpressionBacterialMutationPromoterAvailable sinceSept. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pVRc27_4265
Plasmid#49745DepositorInsertAS27_4265
UseSynthetic BiologyTagsExpressionBacterialMutationCodon optimized for E.coliPromoterpLuxAvailable sinceJan. 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pVRc14_1324
Plasmid#49734DepositorInsertAS14_1324
UseSynthetic BiologyTagsExpressionBacterialMutationCodon optimized for E.coliPromoterpLuxAvailable sinceJan. 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pVRc38_1322
Plasmid#49752DepositorInsertAS38_1322
UseSynthetic BiologyTagsExpressionBacterialMutationCodon optimized for E.coliPromoterpLuxAvailable sinceJan. 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pETduet+p300HAT
Plasmid#157793PurposeExpression of p300 HAT domainDepositorInsertp300HAT (EP300 Human)
UseTagsHis6ExpressionBacterialMutationPromoterT7Available sinceAug. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
hBACCS2-IRES-mCherry
Plasmid#72892PurposeMammalian expression of hBACCS2, a human blue light-activated Ca2+ channel switch, and mCherryDepositorInserthBACCS2-IRES-mCherry
UseTagsIRES-mCherryExpressionMammalianMutationPromoterCMVAvailable sinceJan. 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGL4.10/RSV_SF3B3-miniXon_Luciferase
Plasmid#174660PurposePlasmid containing the SF3B3-miniXon/Firefly luciferase cassette. Translation of Firefly luciferase is controlled by splicing modulation of the SF3B3-miniXon by LMI070.DepositorInsertminiXon cassette
UseLuciferaseTagsExpressionMammalianMutationKozak ATG initiation sequence in middle exonPromoterRSVAvailable sinceJan. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGL4.10/RSV_SF3B3-Xon_Luciferase
Plasmid#174659PurposePlasmid containing the SF3B3-Xon/Firefly luciferase cassette. Translation of Firefly luciferase is controlled by splicing modulation of the SF3B3-xon by LMI070.DepositorInsertXon cassette
UseLuciferaseTagsExpressionMammalianMutationKozak ATG initiation sequence in middle exonPromoterRSVAvailable sinceJan. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
hBACCS2-IRES-GFP
Plasmid#72891PurposeMammalian expression of hBACCS2, a human blue light-activated Ca2+ channel switch, and GFPDepositorInserthBACCS2-IRES-GFP
UseTagsIRES-GFPExpressionMammalianMutationPromoterCMVAvailable sinceJan. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-GNPTAB
Plasmid#78108PurposeMammalian expression of human GNPTAB with C terminal myc tagDepositorInsertGNPTAB (GNPTAB Human)
UseTagsMycExpressionMammalianMutationPromoterCMVAvailable sinceMay 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pUTKan-3xHA-GFP-TOM5
Plasmid#130674PurposeUbiquitously expresses 3xHA-GFP-TOM5 in the outer mitochondrial membrane of plantaDepositorInsertTranslocase of outer mitochondrial membrane 5 (TOM5 Mustard Weed)
UseTags3xHA and sGFPExpressionPlantMutationPromoterUbiquitin10Available sinceSept. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-CAG-sbGLuc-hGtARC2-EYFP
Plasmid#114111Purposefusion protein of Gaussia luciferase variant slow burn, Guillardia theta anion channelrhodopsin-2, and enhanced yellow fluorescent protein for bioluminescent optogeneticsDepositorInsertGaussia luciferase, slow burn; Guillardia theta anion channelrhodopsin-2, human codon optimized
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceAug. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPHR-iRFP713
Plasmid#103818PurposeSoluble BLInCR mock effector that is recruited to 'localizer' sites upon blue light illuminationDepositorInsertPHR-iRFP713 (CRY2 Mustard Weed)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDGB1alpha2_SulR
Plasmid#186425Purposetranscriptional unit for sulfadiazine resistance; plant expression driven by the Pnos promoterDepositorInsertSulR
UseSynthetic Biology; Binary vector for escherichia …TagsExpressionPlantMutationBsaI and BsmBI sites removedPromoterPnosAvailable sinceJan. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAGGS-Flex-GFP
Plasmid#197883PurposeCan be used to generate AAV virus that will express GFP in the presence of CreDepositorInsertGFP
UseAAVTagsExpressionMutationN/APromoterCAGAvailable sinceMarch 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
dmBACCS2VL-IRES-dOrai
Plasmid#67629PurposeMammalian expression of dmBACCS2VL, a modified Drosophila melanogaster blue light-activated Ca2+ channel switch, and dOraiDepositorInsertdmBACCS2VL-IRES-dOrai
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET30a-SRAP-delCys2
Plasmid#84568PurposeEncodes murine SRAP domain (250aa) of Hmces/Srap1 with deletion of catalytic Cys2DepositorInsertSrap1 (Hmces Mouse)
UseTags6xHis and S-TagExpressionBacterialMutationdeletion of Cys2PromoterT7Available sinceNov. 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
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pCDH-AtAFB2-mTagBFP2
Plasmid#179889PurposeExpresses AtAFB2 (auxin-inducible degron system) fused to mTagBFP2 (lentiviral plasmid)DepositorInsertAtAFB2 (AFB2 Synthetic, Mustard Weed)
UseLentiviralTagsmTagBFP2ExpressionBacterial and MammalianMutationPromoterEF1Available sinceMay 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
dmBACCS2NS-IRES-dOrai
Plasmid#67628PurposeMammalian expression of dmBACCS2NS, a modified Drosophila melanogaster blue light-activated Ca2+ channel switch, and dOraiDepositorInsertdmBACCS2NS-IRES-dOrai
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pPHR-EYFP
Plasmid#103817PurposeSoluble BLInCR mock effector that is recruited to 'localizer' sites upon blue light illuminationDepositorInsertPHR-EYFP (CRY2 Mustard Weed)
UseTagsEYFPExpressionMammalianMutationPromoterCMVAvailable sinceNov. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1-EGFP-Vasa open (K295N) (RR228)
Plasmid#206435PurposeVasa expression in Schneider cells for imagingDepositorInsertVasa (vas Fly)
UseTagsEGFPExpressionInsectMutationK282NPromoterAvailable sinceJuly 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_STC2_WT_V5
Plasmid#82957PurposeGateway Donor vector containing STC2, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.DepositorInsertSTC2 (STC2 Human)
UseGateway entry vectorTagsExpressionMutationPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
BmR1_04g07470-bio-His
Plasmid#108037PurposeExpresses enzymatically monobiotinylated full-length BmR1_04g07470 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.DepositorInsertBmR1_04g07470
UseTagsratCD4d3+4,biotinylation sequence, 6xHisExpressionMammalianMutationall potential N-linked glycosylation sites (N-X-S…PromoterCMVAvailable sinceOct. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
HyperADAR control
Plasmid#166969PurposeExpress the Hyperactive catalytic domain of Drosophila ADAR and linker at the 5' end of this domain, in the MSCV-IRES-GFPDepositorInsertlinker-hyperADAR
UseRetroviralTagslinker-HyperADARExpressionMammalianMutationPromoterMSCVAvailable sinceMarch 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
MSI2-DCD
Plasmid#166968PurposeExpress the fusion protein of human MSI2 and the catalytic dead domain of Drosophila ADARDepositorInsertMSI2-DCD
UseRetroviralTagsMSI2-DCDExpressionMammalianMutationN495SPromoterMSCVAvailable sinceMarch 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
MSI2-ADA
Plasmid#166967PurposeExpress the fusion protein of human MSI2 and the hyperactive catalytic domain of Drosophila ADARDepositorInsertMSI2-ADA
UseRetroviralTagsMSI2-ADAExpressionMammalianMutationPromoterMSCVAvailable sinceMarch 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
BMR1_01g02031-bio-His
Plasmid#108116Purpose[Edit] Expresses enzymatically monobiotinylated full-length BMR1_01g02031 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tagDepositorInsertBMR1_01g02031
UseTagsratCD4d3+4,biotinylation sequence, 6xHisExpressionMammalianMutationall potential N-linked glycosylation sites (N-X-S…PromoterCMVAvailable sinceOct. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
nLuc_AP10_PPVs_P9mS
Plasmid#119302PurposeExpresses N-terminus of split luciferase fused to antiparallel coiled-coil AP10 connected with autoinhibitory coil P9mS and PPVs cleavage site in antiparallel harpin linker/for SPOC logicDepositorInsertsplit nLuc fused to antiparallel Coiled-coil AP10 connected with P9mS via PPV cleavable linker
UseLuciferaseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTER E2F2shRNA human
Plasmid#66884PurposeDoxycycline-regulated mammalian expression vector for expressing shRNA against E2F2DepositorInsertE2F transcription factor 2 (E2F2 Human)
UseRNAiTagsExpressionMammalianMutationPromoterH1 TetAvailable sinceJune 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
nLuc_AP4_TEVs_P3mS
Plasmid#119299PurposeExpresses N-terminus of split luciferase fused to antiparallel coiled-coil AP4 connected with autoinhibitory coil P3mS and TEV cleavage site in antiparallel harpin linker/for SPOC logicDepositorInsertsplit nLuc fused to antiparallel Coiled-coil AP4 connected with P3 via TEV cleavable linker
UseLuciferaseTagsMyc tagExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
BMR1_02g00320-bio-His
Plasmid#108028PurposeExpresses enzymatically monobiotinylated full-length BMR1_02g00320 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.DepositorInsertBMR1_02g00320
UseTagsratCD4d3+4,biotinylation sequence, 6xHisExpressionMammalianMutationall potential N-linked glycosylation sites (N-X-S…PromoterCMVAvailable sinceOct. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
BMR1_02g04075-bio-His
Plasmid#108041PurposeExpresses enzymatically monobiotinylated full-length BMR1_02g04075 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.DepositorInsertBMR1_02g04075
UseTagsratCD4d3+4,biotinylation sequence, 6xHisExpressionMammalianMutationall potential N-linked glycosylation sites (N-X-S…PromoterCMVAvailable sinceOct. 12, 2018AvailabilityAcademic Institutions and Nonprofits only