19,798 results

Showing: 11581 - 11610 of 19798 results

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pcDNA3-Delta(Pro-MP)ADAM10-HA

Plasmid

#65107

Purpose

Expression of HA tagged ADAM10 lacking prodomain and metalloprotease domain in mammalian cells

Depositor

Axel Ullrich

Article

Gschwind et al EMBO J. 2003 May 15;22(10):2411-21.

Insert

Delta(Pro-MP)ADAM10 (ADAM10 Human)

Tags

HA

Expression

Mammalian

Mutation

deleted AA 19-455

Promoter

CMV

Available Since

Nov. 7, 2016

Availability

Academic Institutions and Nonprofits only
pCS2+mScarlet-C Cloning Vector

Plasmid

#128147

Purpose

To express a protein of interest fused to the C-terminus of mScarlet

Depositor

Ken-Ichi Takemaru

Type

Empty backbone

Tags

mScarlet fusion

Expression

Mammalian

Available Since

July 2, 2019

Availability

Academic Institutions and Nonprofits only

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SIVu

Plasmid

#81089

Purpose

Single Integration Vector URA - Cloning vector for single integration into the URA3 locus of S.cerevisiae containing two MCS.

Depositor

Serge Pelet

Article

Wosika et al Mol Genet Genomics. 2016 Dec;291(6):2231

Type

Empty backbone

Use

Cre/Lox

Expression

Yeast

Available Since

Nov. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pLVX-TRE3G-BCCP-PupE-IRES-BFP

Plasmid

#113405

Purpose

Express inducible BCCP-PupE in mammalian cell

Depositor

Min Zhuang

Article

Liu et al Nat Methods. 2018 Aug 13. pii: 10.1038/s

Insert

BCCP-PupE-IRES-BFP

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 16, 2018

Availability

Academic Institutions and Nonprofits only

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IRES-puro-GFP

Plasmid

#16616

Depositor

Bert Vogelstein

Article

Torrance et al Nat Biotechnol. 2001 Oct . 19(10):940-5.

Insert

GFP

Expression

Mammalian

Available Since

March 10, 2008

Availability

Academic Institutions and Nonprofits only

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pCMVTAG-NEMO

Plasmid

#11970

Depositor

Jon Ashwell

Article

Wu et al Nat Cell Biol. 2006 Apr . 8(4):398-406.

Insert

NEMO (IKBKG Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 21, 2006

Availability

Academic Institutions and Nonprofits only

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pAAV-mCAMK-RFP

Plasmid

#22908

Depositor

Edward Callaway

Article

Nathanson et al Neuroscience. 2009 Jun 30. 161(2):441-50

Insert

mouse alpha-calcium/calmodulin-dependent protein kinase II promoter (Camk2a Mouse)

Use

AAV

Expression

Mammalian

Available Since

May 25, 2010

Availability

Academic Institutions and Nonprofits only

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plenti-UBC-HA-gate-pGK-HYG

Plasmid

#107396

Purpose

Lentiviral gateway destination vector

Depositor

William Kaelin

Article

Lu et al Science. 2014 Jan 17;343(6168):305-9. do

Type

Empty backbone

Use

Lentiviral; Gateway destination

Tags

HA

Expression

Mammalian

Available Since

May 22, 2018

Availability

Academic Institutions and Nonprofits only

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pCDHblast MCSNard OST-LMNAd50

Plasmid

#22662

Depositor

Tom Misteli

Article

Pegoraro et al Nat Cell Biol. 2009 Oct . 11(10):1261-7.

Insert

LaminAd50 (=Progerin)

Use

Lentiviral

Tags

Onestrep Tag

Expression

Mammalian

Mutation

50AA deletion creating Progerin instead of mature…

Available Since

Dec. 29, 2009

Availability

Academic Institutions and Nonprofits only

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pLEX307-TMPRSS2-G418

Plasmid

#158459

Purpose

Constitutive lentiviral expression of human TMPRSS2.

Depositor

Alejandro Chavez, Sho Iketani

Insert

TMPRSS2 (TMPRSS2 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Sept. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pSJ1256 (pFA6-link-yGFP1-10-CaURA3MX)

Plasmid

#86419

Purpose

C-terminal PCR tagging module for yeast codon optimized split GFP1-10

Depositor

Sue Jaspersen

Article

Smoyer et al J Cell Biol. 2016 Nov 21;215(4):575-590.

Insert

GFP1-10

Expression

Yeast

Available Since

Feb. 14, 2017

Availability

Academic Institutions and Nonprofits only

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FLAG-SENP6

Plasmid

#18065

Depositor

Edward Yeh

Article

Dou et al Mol Cell. 2010 Aug 13. 39(3):333-45.

Insert

SENP6 (SENP6 Human)

Tags

FLAG

Expression

Mammalian

Available Since

May 16, 2008

Availability

Academic Institutions and Nonprofits only
pLemir9-124

Plasmid

#31779

Depositor

Jerry Crabtree

Article

Yoo et al Nature. 2011 Jul 13. doi: 10.1038/nature

Insert

miR-9/9* and miR-124 (Mir124a-2, Mir9-3 Mouse)

Use

Lentiviral

Available Since

Sept. 22, 2011

Availability

Academic Institutions and Nonprofits only

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pJJB308

Plasmid

#107699

Purpose

AtU6 Promoter-LwaCas13a-gRNA Scaffold

Depositor

Daniel Voytas

Article

Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

Type

Empty backbone

Expression

Plant

Available Since

May 17, 2018

Availability

Academic Institutions and Nonprofits only

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pK-myc-Par3b

Plasmid

#19388

Depositor

Ian Macara

Article

Joberty et al Nat Cell Biol 2000 Aug;2(8):531-9.

Insert

Partitioning-defective protein 3b (PARD3 Human)

Tags

Myc

Expression

Mammalian

Mutation

Full length, isoform b.

Available Since

March 31, 2009

Availability

Academic Institutions and Nonprofits only

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p-dCas9-LSD1-Hygro

Plasmid

#104406

Purpose

transient expression of dCas9-LSD1 fusion protein

Depositor

Stephan Beck

Insert

LSD1 (KDM1A Human)

Use

CRISPR

Expression

Mammalian

Mutation

IRATp970A0364D (Source Bioscience) has a P405H ch…

Promoter

CMV promoter

Available Since

April 2, 2020

Availability

Academic Institutions and Nonprofits only

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Opto-mFGFR1_204

Plasmid

#58745

Purpose

Expresses Opto-mFGFR1 (light-activated receptor tyrosine kinase)

Depositor

Harald Janovjak

Article

Grusch et al EMBO J. 2014 Jul 1. pii: e201387695.

Insert

Opto-mFGFR1

Expression

Mammalian

Available Since

Aug. 11, 2014

Availability

Academic Institutions and Nonprofits only

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pBT128c

Plasmid

#138512

Purpose

P2

Depositor

David Liu

Article

Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

Insert

inteinC-dSpCas9

Use

Synthetic Biology

Available Since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

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mKate2-P2A-APEX2-GBP

Plasmid

#67650

Purpose

Mammalian expression vector driving APEX2-GBP (EM peroxidase marker fused to GFP-targeting nanobody). Also contains bicistronially expressed cytoplasmic red fluorescent reporter.

Depositor

Rob Parton

Article

Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

Insert

APEX2-GBP

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 30, 2015

Availability

Academic Institutions and Nonprofits only

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pYPQ141B

Plasmid

#69291

Purpose

Gateway entry vector; single gRNA under AtU3 promoter

Depositor

Yiping Qi

Article

Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

Type

Empty backbone

Use

CRISPR

Available Since

Sept. 14, 2015

Availability

Academic Institutions and Nonprofits only

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Adeno Cas9

Plasmid

#64072

Purpose

Adenoviral vector for the expression of the Cas9

Depositor

Andrea Ventura

Article

Maddalo et al Nature. 2014 Dec 18;516(7531):423-7. doi

Type

Empty backbone

Use

Adenoviral

Tags

FLAG

Promoter

U6 and CBh

Available Since

June 26, 2015

Availability

Academic Institutions and Nonprofits only

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pCSCX

Plasmid

#63918

Purpose

Expression of the sucrose utilization phenotype

Depositor

Claudia Vickers

Article

Bruschi et al Biotechnol Adv. 2012 Sep-Oct;30(5):1001-

Insert

cscAKB

Use

Bacterial cloning vector

Available Since

May 19, 2015

Availability

Academic Institutions and Nonprofits only

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pX458-DsRed2

Plasmid

#112219

Purpose

Cloning backbone for sgRNA, co-expresses human optimized S. pyogenes Cas9 and DsRed2

Depositor

Yinming Liang

Article

Luo et al J Biotechnol. 2018 Jun 7;281:11-20. doi:

Insert

DsRed2

Use

CRISPR

Expression

Mammalian

Mutation

DsRed2 contains a silent nucleotide substitution …

Available Since

July 11, 2018

Availability

Academic Institutions and Nonprofits only

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pRK5-HA-Ubiquitin-K11

Plasmid

#121152

Purpose

Mammalian expression of HA tagged ubiquitin with K11 only, other lysines mutated to arginines

Depositor

Josef Kittler

Article

Birsa et al J Biol Chem. 2014 May 23;289(21):14569-8

Insert

Ubiquitin C (UBC Human)

Expression

Mammalian

Mutation

All Ks apart from K11 mutated to Rs

Available Since

Feb. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pAV-U6+27-Tornado-BroccoliSamSensor#1

Plasmid

#124363

Purpose

Overexpression of circular RNA (Broccoli-based SAM sensor)

Depositor

Samie Jaffrey

Article

Litke et al Nat Biotechnol. 2019 Apr 8. pii: 10.1038

Insert

Tornado-BroccoliSamSensor#1

Expression

Mammalian

Promoter

U6+27

Available Since

June 7, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV2.5-THP-dsRed2

Plasmid

#80335

Purpose

Fluorescent Reporter for Dopaminergic Neuron Differentiation

Depositor

Kwang-Soo Kim

Article

Oh et al Gene Ther. 2009 Mar;16(3):437-40. doi: 1

Insert

rat TH promoter (Th Rat)

Use

AAV

Promoter

rat Tyrosine hydroxylase

Available Since

Oct. 21, 2016

Availability

Academic Institutions and Nonprofits only

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pY094

Plasmid

#84743

Purpose

Expresses huAsCpf1-T2A-GFP and crRNA guide

Depositor

Feng Zhang

Article

Zetsche et al Nat Biotechnol. 2017 Jan;35(1):31-34. do

Inserts

huAsCpf1
GFP

Tags

3xHA, NLS, and T2A

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 25, 2017

Availability

Academic Institutions and Nonprofits only

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YEE-BE4max

Plasmid

#138157

Purpose

C-to-T base editor

Depositor

David Liu

Article

Doman et al Nat Biotechnol. 2020 Feb 10. pii: 10.103

Insert

rAPOBEC1(YEE)-nSpCas9-UGI-UGI

Tags

NLS

Expression

Mammalian

Mutation

Within rAPOBEC1 W90Y + R126E + R132E; within Cas9…

Promoter

CMV

Available Since

Feb. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pME-mTagBFP-CAAX

Plasmid

#75149

Purpose

Membrane targeted TagBFP (blue) fluorophore for use with 5’ promoters.

Depositor

Nicholas Cole

Article

Don et al Zebrafish. 2016 Sep 15.

Insert

mTagBFP-CAAX

Use

Middle entry vector for multisite gateway three f…

Promoter

n/a

Available Since

Oct. 6, 2016

Availability

Academic Institutions and Nonprofits only

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NPY2R-Tango

Plasmid

#66454

Purpose

Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

Depositor

Bryan Roth

Article

Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

Insert

NPY2R (NPY2R Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 15, 2015

Availability

Academic Institutions and Nonprofits only

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19,798 results