We narrowed to 22,938 results for: TES

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Tet-myr-CSK-GFP

Plasmid

#83470

Purpose

Lentiviral vector for doxycycline-inducible expression of membrane targeted-CSK in mammalian cells

Depositor

Barry Gumbiner

Article

Kim et al J Cell Biol. 2015 Aug 3;210(3):503-15. d

Insert

CSK (CSK Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Promoter

doxycycline-inducible promoter

Available Since

Oct. 7, 2016

Availability

Academic Institutions and Nonprofits only

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pCDH-CMV-HA-CFP-SLMAP (isoform r)

Plasmid

#235684

Purpose

Express CFP tagged SLMAP gene in mammalian cells using the CMV promoter

Depositor

Duojia Pan

Article

Wang et al Cell. 2022 Nov 10;185(23):4376-4393.e18.

Insert

SLMAP (SLMAP Human)

Use

Lentiviral

Tags

HA-CFP

Expression

Mammalian

Available Since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

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pETM41_KPNA4_Arm

Plasmid

#178478

Purpose

Bacterial expression of human domain with His-tag and MBP tag

Depositor

Ylva Ivarsson

Article

Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

Insert

KPNA4_Arm (KPNA4 Human, Synthetic)

Tags

His-MBP

Expression

Bacterial

Promoter

T7/LacO

Available Since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

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pCDH-CMV-V5-RFP-MST2

Plasmid

#235688

Purpose

Express RFP tagged MST2 gene in mammalian cells using the CMV promoter

Depositor

Duojia Pan

Article

Wang et al Cell. 2022 Nov 10;185(23):4376-4393.e18.

Insert

MST2 (STK3 Human)

Use

Lentiviral

Tags

V5-RFP

Expression

Mammalian

Available Since

April 28, 2025

Availability

Academic Institutions and Nonprofits only

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pDB2-hCRY2-Halo-CD4-bla

Plasmid

#189986

Purpose

Donor Vector to integrate HaloTag C-terminal into the human CRY2 Locus to express it as a fusion protein

Depositor

Achim Kramer

Article

Gabriel et al Proc Natl Acad Sci U S A. 2024 Aug 20;12

Insert

HaloTag

Use

CRISPR

Tags

3xFlag

Available Since

Feb. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pBabe-lincRNA-RoR

Plasmid

#45763

Depositor

George Daley

Article

Loewer et al Nat Genet. 2010 Dec;42(12):1113-7. Epub

Insert

lincRNA-RoR cDNA (LINC-ROR Human)

Use

Retroviral

Expression

Mammalian

Available Since

Aug. 12, 2013

Availability

Academic Institutions and Nonprofits only

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EE-LRRK2

Plasmid

#193583

Purpose

Expresses full-length LRRK2 with FYVE-CC2 domains from HRS and 3xFLAG on the N-terminus

Depositor

Mark Cookson

Article

Kluss et al Proc Natl Acad Sci U S A. 2022 Oct 25;11

Insert

Leucine-rich repeat kinase 2 (LRRK2 Human)

Tags

FYVE-CC2 and 3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pDIV537

Plasmid

#177704

Purpose

Plasmid expressing optimized Cas9 and NAT marker and sgRNA targeting KU70 homolog in D. hansenii

Depositor

Uffe Mortensen

Article

Strucko et al Synth Biol (Oxf). 2021 Oct 12;6(1):ysab0

Inserts

Cas9
Nat
tRNA-sgRNA-tRNA

Use

CRISPR

Tags

SV40

Expression

Bacterial and Yeast

Promoter

RNR2p (Debaryomyces hansenii ), TDH3p (Candida lu…

Available Since

March 11, 2022

Availability

Academic Institutions and Nonprofits only

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pDEST_FLAG-HUWE1_delta169-189

Plasmid

#187136

Purpose

mammalian cell expression of FLAG-HUWE1 delta169-189

Depositor

Eric Fischer

Article

Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

Insert

HUWE1 (HUWE1 Human)

Tags

1xFLAG

Expression

Mammalian

Mutation

delta169-189

Available Since

Aug. 16, 2022

Availability

Academic Institutions and Nonprofits only

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3XFlag pCMV5-TOPO TAZ (∂WW)

Plasmid

#24811

Depositor

Jeff Wrana

Article

Varelas et al Nat Cell Biol. 2008 Jul . 10(7):837-48.

Insert

TAZ (WWTR1 Human)

Tags

3XFlag

Expression

Mammalian

Mutation

Deletion of amino acids 111-158.

Available Since

May 14, 2010

Availability

Industry, Academic Institutions, and Nonprofits
DNMT3L-dCas9

Plasmid

#126587

Purpose

DNMT3L fused to N-terminus of dCas9; pCDNA3 vector backbone, mammalian expression

Depositor

David Segal

Article

O'Geen et al Epigenetics Chromatin. 2019 May 3;12(1):

Insert

DNMT3L (DNMT3L Human)

Use

CRISPR

Tags

3XFLag-NLS-DNMT3L-dCas9-NLS

Expression

Mammalian

Available Since

Dec. 12, 2021

Availability

Academic Institutions and Nonprofits only

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NSP4-WT-Flag

Plasmid

#210339

Purpose

Expresses SARS-CoV-2 NSP4 fused with Flag in mammalian cells

Depositor

Michele Pagano

Article

Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

Insert

SARS-CoV-2 NSP4 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1, Human)

Tags

Flag

Expression

Mammalian

Mutation

Many synonymous changes due to codon optimization

Available Since

Jan. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pAFW-Ago2

Plasmid

#50554

Purpose

Expression of FLAG tagged Ago2 in S2 cell

Depositor

Yukihide Tomari

Article

Iwasaki et al Mol Cell. 2010 Jul 30;39(2):292-9. doi:

Insert

Argonaute2 (AGO2 Fly)

Tags

FLAG

Expression

Insect

Promoter

Actin5C

Available Since

Feb. 10, 2014

Availability

Academic Institutions and Nonprofits only

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pCS2-mkgDUX4

Plasmid

#21156

Depositor

Stephen Tapscott

Article

Snider et al Hum Mol Genet. 2009 Jul 1;18(13):2414-30

Insert

DUX4 (DUX4 Human)

Expression

Bacterial and Mammalian

Available Since

July 22, 2009

Availability

Academic Institutions and Nonprofits only

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pAdTrack-CMV HA ALAS-1

Plasmid

#22746

Depositor

Bruce Spiegelman

Article

Estall et al Proc Natl Acad Sci U S A. 2009 Dec 29. 1

Insert

ALAS-1 (Alas1 Mouse)

Use

Adenoviral

Tags

HA

Expression

Mammalian

Available Since

June 2, 2010

Availability

Academic Institutions and Nonprofits only
ACTIN-SOX2-PGK Cre

Plasmid

#59019

Purpose

Bicistronic Lentivirus expressing sox2 and Cre

Depositor

Trudy Oliver

Article

Mukhopadhyay et al Cell Rep. 2014 Jul 10;8(1):40-49. doi: 1

Insert

Sox2 (Sox2 Mouse)

Use

Lentiviral

Promoter

B-actin

Available Since

Sept. 17, 2014

Availability

Academic Institutions and Nonprofits only
AA304

Plasmid

#216072

Purpose

Fragmid fragment: (guide cassette) guide expression

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

U6_v1; BsmBI_v0; BsmBI_v1; trRNA_v1 [Sa]; terminator_v1

Use

CRISPR; Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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EW410 IKZF1tar x8-H2B-GFP

Plasmid

#236156

Purpose

Fuorescent reporter encoding H2B-GFP fusion under control of E1b minimal promoter with eight IKZF1 binding sites upstream

Depositor

Xiaojing Gao

Article

Wolfsberg et al Cell Syst. 2025 Jul 16;16(7):101299. doi

Insert

H2B (H2BC11 Human)

Tags

EGFP

Expression

Mammalian

Promoter

E1b minimal promoter with eight IKZF1 binding sit…

Available Since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

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BII-ChBtW-IGF2

Plasmid

#133374

Purpose

Piggybac vector for constitutive expression

Depositor

Blair Madison

Article

Fischer et al Am J Physiol Gastrointest Liver Physiol.

Insert

IGF2 (IGF2 Human)

Tags

Flag

Expression

Mammalian

Mutation

N/A

Promoter

CMV enhancer and hEf1a (CpG free)

Available Since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pPW3754 : pUC-SpCas9_gRNA-HsIRE1-Cterm

Plasmid

#185676

Purpose

SpCas9 and gRNA targeting the C-terminus of HsIRE1

Depositor

Peter Walter

Article

Belyy et al Elife. 2022 Jun 22;11:e74342. doi: 10.75

Insert

ERN1 gRNA (ERN1 Human)

Use

CRISPR

Available Since

March 14, 2023

Availability

Academic Institutions and Nonprofits only
pAAV.hSyn1.Twitch2B.WPRE.SV40

Plasmid

#100040

Purpose

AAV expression of Twitch2B driven from hSyn1 promoter. Twitch 2B is a fluorescent reporter for calcium signaling

Depositor

Oliver Griesbeck

Article

Thestrup et al Nat Methods. 2014 Jan 5. doi: 10.1038/nm

Has Service

AAV1

Insert

Twitch2B

Use

AAV

Expression

Mammalian

Promoter

hSyn1

Available Since

Jan. 31, 2018

Availability

Academic Institutions and Nonprofits only

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pGL410-BRN2p

Plasmid

#110733

Purpose

Luciferase reporter for the human BRN2 promoter

Depositor

Robert Judson-Torres

Article

Zeng et al Cancer Cell. 2018 Jul 9;34(1):56-68.e9.

Insert

BRN2 promoter luciferase (POU3F2 Human)

Use

Luciferase

Expression

Mammalian

Available Since

July 25, 2018

Availability

Academic Institutions and Nonprofits only

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RCASBP(A)-3xFlag-mHoxd13wt

Plasmid

#145288

Purpose

Avian Expression of murine wildtype Hoxd13 with N-terminal 3xFlag tag

Depositor

Denes Hnisz

Article

Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

Insert

Hoxd13(wt) (Hoxd13 Mouse)

Use

Viral avian expression

Tags

3xFlag

Available Since

Sept. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pICH47822_Sr62NLRa

Plasmid

#233537

Purpose

Expresses Sr62NLRa in planta

Depositor

Brande Wulff

Article

Chen et al Science. 2025 Mar 28;387(6741):1402-1408

Insert

Sr62NLRa

Expression

Plant

Promoter

35S

Available Since

May 8, 2025

Availability

Academic Institutions and Nonprofits only

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LITE2.0 EF1a_TALE(Neurog2)-GS-CIB1(NLS*,∆318-334)_WPRE_hGHpA

Plasmid

#47458

Purpose

LITE2.0 TALE-CIB1. Changes from LITE1.0: glycine-serine linker, mutated endogenous NLS, aa318-334 deletion. This particular TALE targeted to Neurog2. EF1-alpha promoter for ubiquitous mammalian exp.

Depositor

Feng Zhang

Article

Konermann et al Nature. 2013 Jul 23. doi: 10.1038/nature

Insert

TALE (Neurog2)-GS-CIB1(NLS*,∆318-334)

Tags

HA

Expression

Mammalian

Promoter

EF1-alpha

Available Since

Oct. 22, 2013

Availability

Academic Institutions and Nonprofits only

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CaMKIV-dCT

Plasmid

#126422

Purpose

Plasmid expresses constitutively active form of CaMK IV (C-terminal regulatory domains removed making the enzyme constitutively active)

Depositor

Douglas Black

Article

Xie et al Nature. 2001 Apr 19;410(6831):936-9. doi

Insert

Calcium/calmodulin-dependent protein kinase type IV (CAMK4 Human)

Tags

FLAG

Expression

Mammalian

Available Since

July 12, 2019

Availability

Academic Institutions and Nonprofits only

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aMHC-Puro Rex-Blast

Plasmid

#21231

Depositor

Mark Mercola

Article

Kita-Matsuo et al PLoS ONE. 2009 . 4(4):e5046.

Insert

alpha myosin heavy chain promoter

Use

Lentiviral

Available Since

July 22, 2009

Availability

Academic Institutions and Nonprofits only

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p-mCherry-C1-hCRACR2A-isoform-a

Plasmid

#79593

Purpose

Transient expression

Depositor

Yousang Gwack

Article

Srikanth et al Sci Signal. 2016 Mar 22;9(420):ra31. doi

Insert

CRACR2A-isoform-a (CRACR2A Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

July 29, 2016

Availability

Academic Institutions and Nonprofits only
pCR8/GW_TOPO_Cdk12_Untagged

Plasmid

#127176

Purpose

Untagged mouse Cdk12 transgene (NM_001109626.1 isoform) cloned into Gateway entry vector

Depositor

Phil Sharp

Article

Dubbury et al Nature. 2018 Dec;564(7734):141-145. doi:

Insert

Mouse Cyclin Dependent Kinase 12 (Cdk12 Mouse)

Use

Entry vector with transgene for gateway cloning

Tags

None

Promoter

None

Available Since

July 5, 2019

Availability

Academic Institutions and Nonprofits only

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pCEP4-BG505.SOSIP-TM

Plasmid

#111844

Purpose

Mammalian expression plasmid for BG505 SOSIP.664 fused to a transmembrane tether

Depositor

Erik Procko

Article

Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

Insert

HIV-1 Env (BG505 SOSIP.664)

Tags

CD5 leader sequence and TM helix from MHC class I

Expression

Mammalian

Mutation

Codon-optimized synthetic gene; has SOSIP mutatio…

Promoter

CMV

Available Since

March 25, 2019

Availability

Academic Institutions and Nonprofits only

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