We narrowed to 13,692 results for: Egf

1. pLib6.4-Aalb_744

   Plasmid

   #176663

   Purpose

   Expression of sgRNA under Ae. albopictus U6 (AALF029744) promoter & delivery of D.mel-Actin5C::EGFP-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

   Depositor

   Norbert Perrimon

   Article

   Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

   Type

   Empty backbone

   Use

   Crispr

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Nov. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGLTR-FP-GFP

   Plasmid

   #58242

   Purpose

   GATEWAY-compatible lentiviral RNAi delivery vector; SFFV-driven eGFP marker

   Depositor

   Stephan Geley

   Article

   Sigl et al PLoS One. 2014 May 19;9(5):e97764. doi:

   Insert

   attR1-Cm(R)-CcdB-attR2
   

   Use

   Lentiviral and RNAi; Gateway destionation vector

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   no

   Available since

   Nov. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. Ad-CBR (GFP/cAPC)

   Plasmid

   #16576

   Depositor

   Bert Vogelstein

   Article

   Shih et al Cancer Res. 2000 Mar 15. 60(6):1671-6.

   Insert

   cAPC (APC Human)
   

   Use

   Adenoviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Residues 958-2076

   Promoter

   Available since

   July 3, 2008

   Availability

   Academic Institutions and Nonprofits only

4. UAS::PTPRFb-DN-GFP

   Plasmid

   #37111

   Depositor

   Alvaro Sagasti

   Article

   Wang et al Curr Biol. 2012 Mar 6;22(5):373-82. Epub

   Insert

   UAS::PTPRFb-DN-GFP (ptprfb Zebrafish)
   

   Use

   Tol2 gateway expression vector

   Tags

   EGFP

   Expression

   Mutation

   Deleted aa1386-1909 corresponding to phosphatase …

   Promoter

   10xUAS

   Available since

   July 18, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-G12-CheRiff-T2A-mCherry

   Plasmid

   #170275

   Purpose

   CheRiff-T2A-mCherry construct expression in mammlian cells. pAAV vector. Reporter plasmid.

   Depositor

   Vladislav Verkhusha

   Article

   Kaberniuk et al Nat Commun. 2021 Jun 23;12(1):3859. doi:

   Insert

   CheRiff-T2A-mCherry
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   11xUAS with TATA-box minimal promoter

   Available since

   June 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. SL2-sgSat-MTSb/BFP/pdCas9-C1

   Plasmid

   #162761

   Purpose

   Expressing dCas9 and sgRNA containg MTSa targeting centromeres

   Depositor

   Antony K Chen

   Article

   Wu et al Nucleic Acids Res. 2018 Jul 27;46(13):e8

   Insert

   dCas9 and sgRNA(SL2-sgSat-MTSb)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   dCas9(nuclease deactivated Cas9)

   Promoter

   U6/CMV

   Available since

   March 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTS108 pHAGE2 CMVtetO2 MCS-3C-SUMOstar-22xGS-GFP

   Plasmid

   #199361

   Purpose

   Lentiviral transfer plasmid for doxycycline inducible expression of a C-terminally 3C-SUMOstar-GFP-tagged protein in mammalian cells.

   Depositor

   Rebecca Voorhees

   Article

   Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   3C-SUMOstar-EGFP

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   Available since

   April 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTS109 pHAGE2 CMVtetO2 MCS-TEV-SUMOstar-22xGS-GFP

   Plasmid

   #199362

   Purpose

   Lentiviral transfer plasmid for doxycycline inducible expression of a C-terminally TEV-SUMOstar-GFP-tagged protein in mammalian cells.

   Depositor

   Rebecca Voorhees

   Article

   Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   TEV-SUMOstar-EGFP

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   Available since

   April 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLL3.7 shRNA against CRTC2

   Plasmid

   #72711

   Purpose

   Lentiviral expression of shRNA against CRTC2 (3rd generation)

   Depositor

   Freddy Jeanneteau

   Article

   Jeanneteau et al Proc Natl Acad Sci U S A. 2012 Jan 24;10

   Insert

   CRTC2
   

   Use

   Lentiviral

   Tags

   CMV-EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Feb. 12, 2016

   Availability

   Academic Institutions and Nonprofits only

10. pFasBac+GFP-CAMSAP3 CC+CKK

    Plasmid

    #59041

    Purpose

    for baculovirus expression of mouse CAMSAP3 CC+CKK in Sf9 cells

    Depositor

    Ron Vale

    Article

    Hendershott et al Proc Natl Acad Sci U S A. 2014 Apr 22;11

    Insert

    CAMSAP3 CC+CKK domain (Camsap3 Mouse)
    

    Use

    Tags

    His and eGFP

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Aug. 27, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. GFP-Tac

    Plasmid

    #162489

    Purpose

    Expresses Tac (IL2 recptor alpha subunit) with GFP tag in mammalian cells

    Depositor

    Lei Lu

    Article

    Sun et al J Biol Chem. 2020 Aug 21. pii: RA120.014

    Insert

    Tac (IL2RA Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCellFree_G03 ATF1

    Plasmid

    #67065

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    ATF1 (ATF1 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Expression

    Mutation

    Promoter

    T7

    Available since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

13. pJH4329

    Plasmid

    #191679

    Purpose

    Prgef-1-GtACR2::wCherry unc-54 3' UTR C.elegans Pan-neural expression of GtACR2 RFP

    Depositor

    Mei Zhen

    Article

    Lu et al Curr Biol. 2022 Nov 7;32(21):4631-4644.e

    Insert

    GtACR2
    

    Use

    Tags

    wCherry

    Expression

    Worm

    Mutation

    Promoter

    Pregf-1

    Available since

    Nov. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTRE-FDXR wt

    Plasmid

    #16610

    Depositor

    Bert Vogelstein

    Article

    Hwang et al Nat Med. 2001 Oct . 7(10):1111-7.

    Insert

    FDXR (FDXR Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 7, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLib6.4-Cquin_596

    Plasmid

    #176655

    Purpose

    Expression of sgRNA under C. quinquefasciatus U6 (CPIJ039596) promoter & delivery of D.mel-Actin5C::EGFP-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

    Depositor

    Norbert Perrimon

    Article

    Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

    Type

    Empty backbone

    Use

    Crispr

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Nov. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pJOE5751.1

    Plasmid

    #135083

    Purpose

    pBR322-based L-rhamnose-inducible vector.

    Depositor

    Josef Altenbuchner

    Article

    Hoffmann et al FEBS J. 2012 Oct;279(20):3911-24. doi: 1

    Insert

    P-rhaP-BAD-His tag-eGFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. HS-DN-Ect2

    Plasmid

    #105956

    Purpose

    heat shock inducible transgenesis

    Depositor

    Caren Norden

    Article

    Dzafic et al Cell Rep. 2015 Oct 6;13(1):168-182. doi:

    Insert

    Ect2 (ECT2 Human)
    

    Use

    Tags

    EGFP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. p-mCherry-N1-iChloC

    Plasmid

    #85467

    Purpose

    improved slowChloC artificial anion conducting ChR. Codon optimized for mammalian expression (human/mouse)

    Depositor

    Peter Hegemann

    Article

    Wietek et al Sci Rep. 2015 Oct 7;5:14807. doi: 10.103

    Insert

    iChloC
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    E83Q, E90R, E101S, T159C, D156N

    Promoter

    CMV (+enhancer)

    Available since

    Jan. 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCAG Fzd7 GFP

    Plasmid

    #66106

    Purpose

    expression of chicken Fzd7 GFP

    Depositor

    Martin Scaal, Felicitas Proels

    Article

    et al Development. 2015 Feb 15;142(4):665-71.

    Insert

    Fzd7 (FZD7 Chicken)
    

    Use

    Chicken expression

    Tags

    EGFP

    Expression

    Mutation

    Promoter

    CAG

    Available since

    Oct. 19, 2015

    Availability

    Academic Institutions and Nonprofits only

20. pCellFree_G03 ANXA1

    Plasmid

    #67071

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    ANXA1 (ANXA1 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Expression

    Mutation

    Promoter

    T7

    Available since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

21. eDrTrkA

    Plasmid

    #183996

    Purpose

    Optically controlled eDrTrkA expression in mammalian cells

    Depositor

    Vladislav Verkhusha, Dmitry Gerashchenko

    Article

    Leopold et al Nat Methods. 2022 Jul;19(7):871-880. doi

    Insert

    eDrTrkA (NTRK1 Human)
    

    Use

    Tags

    Ig kappa secretory signal, mCherry, Deinococcus r…

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pEntry Tshld(FKBP F36V)-GFP

    Plasmid

    #53260

    Purpose

    pEntry Tshld(FKBP F36V)-GFP is a product of BP recombination reaction between attB flanked Tshld(F36V) system-encoded PCR product and pDonor 201 plasmid.

    Depositor

    Matthew Pratt

    Article

    Lin et al Chembiochem. 2014 Mar 11. doi: 10.1002/c

    Insert

    Tshld(FKBP F36V)-GFP
    

    Use

    Tags

    EGFP, Flag, and HA

    Expression

    Mutation

    FKBP F36V

    Promoter

    Available since

    May 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. RnBarr2-mTurquoise2

    Plasmid

    #137792

    Purpose

    Visualization of beta-arrestin2

    Depositor

    Dorus Gadella

    Article

    Pietraszewska-Bogiel et al ACS Omega. 2020 Feb 3;5(6):2648-2659. do

    Insert

    Rat beta-arrestin2 (Arrb2 Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pB80-FRB-GFP-ppKin14VIb(861-1321)

    Plasmid

    #174638

    Purpose

    Rapalog-inducible coupling to dimeric moss kinesin-14 for retrograde transport via FRB

    Depositor

    Lukas Kapitein

    Article

    Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

    Insert

    FRB-GFP-ppKin14VIb(861-1321)
    

    Use

    Tags

    FRB-GFP

    Expression

    Mammalian

    Mutation

    EGFP: Met1Del; ppKin14VIb(aa861-1321)

    Promoter

    Chicken beta-actin

    Available since

    Nov. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCellFree_G03 STAT3

    Plasmid

    #67120

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    STAT3 (STAT3 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Expression

    Mutation

    Promoter

    T7

    Available since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

26. spa-GCaMP6s

    Plasmid

    #67556

    Purpose

    photoactivatable fluorescent calcium reporter

    Depositor

    Ehud Isacoff

    Article

    Berlin et al Nat Methods. 2015 Jul 13. doi: 10.1038/n

    Insert

    spa-GCaMP6s
    

    Use

    Tags

    HIS-tag

    Expression

    Mammalian

    Mutation

    K65T, I83V, G87S, Y92D, V115H, K118V, S187R, Y196…

    Promoter

    Available since

    July 28, 2015

    Availability

    Academic Institutions and Nonprofits only

27. pCellFree_G03 ISG15

    Plasmid

    #67054

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    ISG15 (ISG15 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Expression

    Mutation

    Promoter

    T7

    Available since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

28. pExT

    Plasmid

    #36889

    Depositor

    Liqun Luo

    Article

    Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

    Inserts

    T (i.e., ATG, start codon as a first exon)
    GFP
    beta-globin intron
    Hygro
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    deleted nucleotides before nucleotide 275 and ins…

    Promoter

    CAG (chicken beta actin promoter and CMV enhancer…

    Available since

    Aug. 8, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pExG

    Plasmid

    #36888

    Depositor

    Liqun Luo

    Article

    Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

    Inserts

    GFP
    tdTomato-3Myc
    beta-globin intron
    Hygro
    

    Use

    Tags

    3 Myc tags

    Expression

    Mammalian

    Mutation

    deleted nucleotides after nucleotide 274, inserti…

    Promoter

    CAG (chicken beta actin promoter and CMV enhancer…

    Available since

    Aug. 17, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. PH-miRFP-FRB

    Plasmid

    #193568

    Purpose

    Expresses PH domain from PLC delta1 tagged with miRFP703 and FRB mutant (T2098L) in mammalian cells.

    Depositor

    Justin Taraska

    Article

    Obashi et al Nat Commun. 2023 Feb 9;14(1):732. doi: 1

    Insert

    PH domain from PLC delta1 (Plcd1 Rat)
    

    Use

    Tags

    FRB (T2098L) and miRFP703

    Expression

    Mammalian

    Mutation

    PH domain

    Promoter

    CMV

    Available since

    Feb. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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