We narrowed to 13,276 results for: cDNA

1. PTPRA-MAC

   Plasmid

   #139632

   Purpose

   MAC-tagged gene expression

   Depositor

   Markku Varjosalo

   Article

   Yadav et al iScience. 2020 Feb 21;23(2):100871. doi:

   Insert

   PTPRA (PTPRA Human)
   

   Use

   Tags

   StrepIII-HA-BirA*

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   May 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. SpyTag-RBD

   Plasmid

   #159999

   Purpose

   Expresses SARS-CoV-2 RBD (340-538) (receptor-binding domain from Spike) with N-terminal SpyTag fusion in mammalian cells

   Depositor

   Mark Howarth

   Article

   Rahikainen et al Angew Chem Int Ed Engl. 2020 Sep 4. doi:

   Insert

   SpyTag-SARS-CoV-2 RBD (340-538) (S SARS-CoV-2)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Nov. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. FLAG-MED7

   Plasmid

   #15416

   Depositor

   Ronald Conaway, Joan Conaway

   Article

   Sato et al J Biol Chem. 2003 Apr 25. 278(17):15123-

   Insert

   MED7 (MED7 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   N/A

   Promoter

   Available Since

   Aug. 15, 2007

   Availability

   Academic Institutions and Nonprofits only

4. Anti-amyloid-β 40/42 nanobody (R3VQ) with sortase tag

   Plasmid

   #233217

   Purpose

   Mammalian epression of anti-amyloid-β 40/42 nanobody (R3VQ) with a sortase tag for direct dye conjugation.

   Depositor

   Jeff Lichtman

   Article

   Han et al bioRxiv 2023.10.24.563674

   Insert

   Anti-amyloid-β 40/42 nanobody (R3VQ)
   

   Use

   Tags

   Sortase tag, His tag

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. Anti-pTau nanobody (A2) with sortase tag

   Plasmid

   #233218

   Purpose

   Mammalian epression of anti-pTau nanobody (A2) with a sortase tag for direct dye conjugation.

   Depositor

   Jeff Lichtman

   Article

   Han et al bioRxiv 2023.10.24.563674

   Insert

   Anti-pTau nanobody (A2)
   

   Use

   Tags

   Sortase tag, His tag

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCD4-TagRFP-T (RHPMP151)

   Plasmid

   #119238

   Purpose

   Expresses CD4 fused to TagRFP-T

   Depositor

   Ricardo Henriques

   Article

   Pereira et al Front Immunol. 2019 Apr 5;10:675. doi: 1

   Insert

   CD4 (CD4 Human)
   

   Use

   Tags

   tagRFP-T

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Feb. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. FLAG-hIes2

   Plasmid

   #15355

   Depositor

   Ronald Conaway, Joan Conaway

   Article

   Jin et al J Biol Chem. 2005 Dec 16. 280(50):41207-

   Insert

   PAP-1 binding protein (INO80B Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 17, 2007

   Availability

   Academic Institutions and Nonprofits only

8. single polypeptide FPX biosensor for caspase-8

   Plasmid

   #60884

   Purpose

   FPX biosensor for caspase-8

   Depositor

   Robert Campbell

   Article

   Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n

   Insert

   single polypeptide FPX biosensor for caspase-8
   

   Use

   Tags

   HindIII site and NES sequence LALKLAGLDIGS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Jan. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

9. pSS1 (hLPL-FLAG)

   Plasmid

   #102447

   Purpose

   Expresses FLAG-tagged human LPL in mammalian cells

   Depositor

   Brandon Davies

   Article

   Chi et al J Biol Chem. 2015 May 8;290(19):11865-77

   Insert

   Lipoprotein lipase (LPL Human)
   

   Use

   Tags

   6x His and FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   May 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. HiBiT-FZD10

    Plasmid

    #195848

    Purpose

    Expresses human FZD10 with an N-terminal HiBiT

    Depositor

    Gunnar Schulte

    Article

    Kozielewicz et al ACS Pharmacol Transl Sci. 2021 May 11;4(

    Insert

    FZD10 (FZD10 Human)
    

    Use

    Tags

    HiBiT

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. B*40:01:01

    Plasmid

    #203257

    Purpose

    Overexpress HLA allele

    Depositor

    Catherine Wu, Derin Keskin

    Article

    Sarkizova et al Nat Biotechnol. 2020 Feb;38(2):199-209.

    Insert

    B*40:01:01 (HLA-B Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. GFP-TRIM66

    Plasmid

    #65400

    Purpose

    GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

    Depositor

    Kyle Miller

    Article

    Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

    Insert

    TRIM66 (TRIM66 Human)
    

    Use

    Tags

    GFP and V5

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available Since

    Aug. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

13. pDEST47-ARL4C-GFP

    Plasmid

    #67402

    Purpose

    Mammalian expression of hArl4c with C-term GFP tag

    Depositor

    Richard Kahn

    Article

    Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

    Insert

    ARL7 (ARL4C Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

14. canxTM-mEmerald (cytosolic)

    Plasmid

    #186962

    Purpose

    Expresses mEmerald connected with the C-terminus of Calnexin transmembrane domain.

    Depositor

    Ke Xu

    Article

    Wang et al Proc Natl Acad Sci U S A. 2022 May 3;119

    Insert

    CANX (CANX Human)
    

    Use

    Tags

    mEmerald

    Expression

    Mammalian

    Mutation

    Only express calnexin (482-end) and add ER signal…

    Promoter

    CMV Promoter

    Available Since

    Sept. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. ss-SNAP-TMD-NS3-Gal4

    Plasmid

    #112279

    Purpose

    Encodes the membrane-targeted TMD-NS3-Gal4, which exhibits drug-inducible transcription "turn-off" activity. SNAP-tag is fused to the extracellular region of the encoded protein construct.

    Depositor

    John Ngo

    Article

    Tague et al Nat Methods. 2018 Jul;15(7):519-522. doi

    Insert

    SNAP-TMD-NS3-Gal4
    

    Use

    Tags

    AU1 (N term on NS3) and HA (C term on NS3)

    Expression

    Mammalian

    Mutation

    NS3 mutant T54A

    Promoter

    CMV

    Available Since

    Sept. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. Beta-Globin-PTC39

    Plasmid

    #130701

    Purpose

    Expresses Beta-globin-PTC39 NMD reporter (It has a Premature termination codon (PTC) at 39th Amino acid)

    Depositor

    Marvin Tanenbaum

    Article

    Hoek et al Mol Cell. 2019 May 23. pii: S1097-2765(1

    Insert

    Beta Globin (HBB Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    PTC39

    Promoter

    Pcmv

    Available Since

    Sept. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. CHRM1-DuET

    Plasmid

    #213207

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    CHRM1 (CHRM1 Human)
    

    Use

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, T7

    Available Since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. hCEP120-Myc

    Plasmid

    #50381

    Purpose

    Tet-On inducible expression of Myc-tagged hCEP120

    Depositor

    Tang Tang

    Article

    Lin et al J Cell Biol. 2013 Jul 22;202(2):211-9. d

    Insert

    Human CEP120 (CEP120 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    This hCEP120 contains 4 SNPs (rs6876883, rs659544…

    Promoter

    CMV-2xTetO

    Available Since

    July 14, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. CaMKIV-dCT

    Plasmid

    #126422

    Purpose

    Plasmid expresses constitutively active form of CaMK IV (C-terminal regulatory domains removed making the enzyme constitutively active)

    Depositor

    Douglas Black

    Article

    Xie et al Nature. 2001 Apr 19;410(6831):936-9. doi

    Insert

    Calcium/calmodulin-dependent protein kinase type IV (CAMK4 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. AXL KD

    Plasmid

    #105934

    Purpose

    Mammalian expression of myc-his tagged AXL kinase dead (K558R) mutant

    Depositor

    Rosa Marina Melillo

    Article

    Avilla et al Cancer Res. 2011 Mar 1;71(5):1792-804. d

    Insert

    AXL (AXL Human)
    

    Use

    Tags

    His and myc

    Expression

    Mammalian

    Mutation

    K558R, kinase dead

    Promoter

    tet-inducible CMV

    Available Since

    Feb. 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pDEST40-2XFL-Src-S4-KD

    Plasmid

    #140297

    Purpose

    Expresses Flag-tagged human Src-S4-KD in mammalian cells for structure-function studies

    Depositor

    Mark Moasser

    Article

    Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

    Insert

    Src deletion mutant (SRC Human)
    

    Use

    Tags

    2X Flag

    Expression

    Mammalian

    Mutation

    contains only the SH4 and KD regions

    Promoter

    CMV

    Available Since

    April 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. PH Akt-Venus

    Plasmid

    #85223

    Purpose

    Venus tagged PH domain of Akt for use as a PIP3 sensor

    Depositor

    Narasimhan Gautam

    Article

    O'Neill et al Mol Biol Cell. 2016 May 1;27(9):1442-50.

    Insert

    PH AKT(1-148) (AKT1 Human)
    

    Use

    Tags

    Venus

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pDEST47-ARL3

    Plasmid

    #67460

    Purpose

    Mammalian expression of hArl3 with stop codon (native expression)

    Depositor

    Richard Kahn

    Article

    Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

    Insert

    ARL3 (ARL3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

24. nLYN267-gs10-NLS-VPR

    Plasmid

    #188265

    Purpose

    Plasmid ensures constitutive expression of the N-terminal fragment of split LYN kinase domain (aa 268-512), fused to the VPR activation domain, a flexible GS linker and the SV40 NLS signal at the C-terminus

    Depositor

    Roman Jerala

    Article

    Rihtar et al Nat Chem Biol. 2022 Sep 26. doi: 10.1038

    Insert

    nLYN267 (LYN Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. B*58:01:01

    Plasmid

    #203261

    Purpose

    Overexpress HLA allele

    Depositor

    Catherine Wu, Derin Keskin

    Article

    Sarkizova et al Nat Biotechnol. 2020 Feb;38(2):199-209.

    Insert

    B*58:01:01 (HLA-B Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCMV/myc/ER-αT9ib

    Plasmid

    #110768

    Purpose

    Expresses ER intrabody against mouse and human TLR9

    Depositor

    Thomas Böldicke

    Article

    Reimer et al Cell Mol Biol Lett. 2013 Sep;18(3):433-4

    Insert

    intrabody against mouse and human TLR9
    

    Use

    Tags

    ER retention signal (SEKDEL), ER signal peptide, …

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Sept. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pDEST40-2XHA-Src-Y419F

    Plasmid

    #140316

    Purpose

    Expresses HA-tagged human Src-Y419F in mammalian cells for structure-function studies

    Depositor

    Mark Moasser

    Article

    Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

    Insert

    Src mutant (SRC Human)
    

    Use

    Tags

    2X HA

    Expression

    Mammalian

    Mutation

    Y419F

    Promoter

    CMV

    Available Since

    April 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pHS18 (mANGPTL3-Strep)

    Plasmid

    #109111

    Purpose

    Expresses StrepII-tagged mouse ANGPTL3 in mammalian cells

    Depositor

    Brandon Davies

    Article

    Chi et al Mol Metab. 2017 Oct;6(10):1137-1149. doi

    Insert

    ANGPTL3 (Angptl3 Mouse)
    

    Use

    Tags

    StrepII

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 5, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

29. Dronpa-DEVD-TagRFPT

    Plasmid

    #87704

    Purpose

    Fluorescent biosensor for super-resolution imaging of caspase activity.

    Depositor

    Jin Zhang

    Article

    Mo et al Nat Methods. 2017 Apr;14(4):427-434. doi

    Insert

    Dronpa-DEVD-TagRFPT
    

    Use

    Tags

    6xHis, T7 tag (gene 10 leader), and Xpress (TM) t…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. A*33:01:01

    Plasmid

    #203288

    Purpose

    Overexpress HLA allele

    Depositor

    Catherine Wu, Derin Keskin

    Article

    Sarkizova et al Nat Biotechnol. 2020 Feb;38(2):199-209.

    Insert

    A*33:01:01 (HLA-A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge