19,805 results
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Plasmid#107712PurposeA somatic channelrhodopsin, which enables single cell, single millisecond resolution optogenetics. Cre-dependent virusDepositorHas ServiceAAV9InsertsoCoChR-GFP
UseAAVTagsEGFP and KA2ExpressionMammalianPromoterhSynapsinAvailable SinceMay 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
NLS-mCherry-NES reporter (pDN160)
Plasmid#72660PurposeNLS-mCherry-NES 17 reporter for light-inducible nuclear export block with pDN157 and pDN158DepositorInsertNLS-mCherry-NES
ExpressionMammalianPromoterCMVAvailable SinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA AXL
Plasmid#105932PurposeMammalian expression of myc-His tagged AXLDepositorAvailable SinceFeb. 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDsRed-Max-N1
Plasmid#21718DepositorInsertDsRed-Max
ExpressionMammalianAvailable SinceAug. 5, 2009AvailabilityAcademic Institutions and Nonprofits only -
C3AR1-Tango
Plasmid#66231PurposeExpression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocationDepositorAvailable SinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
mCherry-Smoothened1-N-18
Plasmid#55134PurposeLocalization: Cilium, Excitation: 587, Emission: 610DepositorInsertSmoothened1
TagsmCherryExpressionMammalianPromoterCMVAvailable SinceAug. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pJFRC150-20XUAS-IVS-Flp1::PEST
Plasmid#32132DepositorInsertFlippase1::PEST
TagsPESTExpressionInsectMutationAspartic Acid at Amino Acid 5Available SinceSept. 23, 2011AvailabilityAcademic Institutions and Nonprofits only -
pB1H2w2-zif268
Plasmid#18045DepositorInsertzif268
ExpressionBacterialMutationZif268 is fused to the omega subunit of RNA polym…Available SinceJune 20, 2008AvailabilityAcademic Institutions and Nonprofits only -
EasyFusion T2A-H2B-TagBFP
Plasmid#113086PurposeGeneral donor vector with T2A-H2B-TagBFP coding sequence flanking by restriction sites for generating knock-in donorDepositorInsertT2A-H2B-TagBFP
UseSynthetic BiologyAvailable SinceAug. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEX-human p53-(1-393)
Plasmid#24860DepositorAvailable SinceAug. 5, 2010AvailabilityAcademic Institutions and Nonprofits only -
pHEE2E-TRI
Plasmid#71288PurposeEgg cell-specific promoter-controlled expression 3×FLAG-NLS-zCas9-NLS and two sgRNAs targeting three genes: ETC2, TRY, and CPCDepositorInsertssgRNA targeting TRY and CPC genes
sgRNA targeting ETC2 gene
zCas9
UseCRISPR; Plant binary vectorTags3x FLAG and NLSExpressionPlantMutationZea mays codon-optimized Cas9PromoterEC1.2 enhancer fused to EC1.1 promoter and U6-26p…Available SinceJan. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CWB-cyan pre-eGRASP(p32)
Plasmid#111579PurposeAn AAV vector that expresses cyan pre-eGRASP(p32) under the CaMKIIa promoter.DepositorInsertcyan pre-eGRASP(p32)
UseAAVPromoterCaMKIIaAvailable SinceJune 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLV-CDH5-mRuby3-IRES-puro
Plasmid#85144Purposelentiviral expression of human VE-cadherin, C-terminally tagged with mRuby3DepositorInsertCDH5-mRuby3 (VE-cadherin, C-terminally tagged with mRuby3 (CDH5 Human)
UseLentiviralTagsmRuby3PromoterCAGAvailable SinceSept. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBS-85xtetO-KlURA3
Plasmid#44757DepositorInsert85x tetO array + KlURA3 marker + CHRI1 homology
Available SinceJuly 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
PKC eta DN
Plasmid#21246DepositorAvailable SinceAug. 13, 2009AvailabilityAcademic Institutions and Nonprofits only -
pTHSSe_59
Plasmid#109253PurposeTALEsp1 stabilized promoter driving GFPDepositorInsertsfGFP
ExpressionBacterialPromoterTALEsp1 stabilized promoterAvailable SinceJuly 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
CMV500 A-C/EBPβ
Plasmid#33363DepositorInsertCebpb (Cebpb Mouse)
TagsFLAGExpressionMammalianMutationDominant Negative (see comments)PromoterCMVAvailable SinceDec. 14, 2011AvailabilityAcademic Institutions and Nonprofits only -
pDiGi
Plasmid#59323PurposeInducible expression of dsRed and GFP in bacteriaDepositorInsertsEGFP
dsRed
ExpressionBacterialPromoterPbad and PlacAvailable SinceOct. 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
pmSTARRseq1
Plasmid#96945PurposeCpG free plasmid for testing effects of methylation on regulatory activityDepositorTypeEmpty backboneUseMstarr-seq screening vectorExpressionMammalianPromoterCpG -free EF1 promoterAvailable SinceAug. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pVITRO1-Trastuzumab-IgM/κ
Plasmid#61881PurposeExpresses HER2/neu receptor specific humanized IgM/κ antibody isotype (Trastuzumab-IgM/κ)DepositorInsertsHER2/neu receptor specific humanized Mu heavy chain expression cassette
HER2/neu receptor specific humanized kappa light chain expression cassette
ExpressionMammalianPromotermEF1 Prom and rEF1 PromAvailable SinceMarch 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET-6His-MBP-MpAgo
Plasmid#79249PurposeThis plasmid encodes the Argonaute protein from Marinitoga Piezophila and is codon optimized for E. coli expressionDepositorInsertMarinitoga piezophila Argonaute
UseCRISPRTags6XHis-tag + MBP + PreScission Cleavage SiteExpressionBacterialPromoterT7Available SinceJuly 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_KRAS_p.G12V
Plasmid#81665PurposeGateway Donor vector containing KRAS,part of the Target Accelerator Plasmid Collection.DepositorAvailable SinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGL3 Zscan4 promoter
Plasmid#69249PurposeLuciferase reporter for Dux4 activation of Zscan4 transcriptionDepositorInsertZscan4 (ZSCAN4 Human)
UseLuciferaseMutationThe two single nucleotide mismatches found betwee…PromoterZscan4Available SinceSept. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
MSCV-EGFP
Plasmid#91975PurposeEGFP reporter cell line control (no miR-19 sites)DepositorInsertEGFP
UseRetroviralExpressionMammalianAvailable SinceAug. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
HA-EglN2-pcDNA3
Plasmid#18961DepositorAvailable SinceJune 19, 2009AvailabilityAcademic Institutions and Nonprofits only -
pGEMHE-membrane-mEGFP
Plasmid#105526PurposeT7 promotor drives in vitro mRNA transcription of a mEGFP-tagged membrane reporterDepositorInsertGNAI2 (GNAI2 Human)
UseIn vitro transcriptionTagsmEGFPMutationfragment encoding the plasma membrane targeting s…PromoterT7Available SinceMarch 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pVITRO1-102.1F10-IgM/λ
Plasmid#61880PurposeExpresses grass pollen allergen Phl p 7 specific human IgM/λ antibody isotype (102.1F10-IgM/λ)DepositorInsertsgrass pollen allergen Phl p 7 specific human Mu heavy chain expression cassette
grass pollen allergen Phl p 7 specific human lambda light chain expression cassette
ExpressionMammalianPromotermEF1 Prom and rEF1 PromAvailable SinceMarch 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
HAT1
Plasmid#25239PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceAug. 13, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
pEGFP-N1-TrkB
Plasmid#32500DepositorAvailable SinceOct. 13, 2011AvailabilityAcademic Institutions and Nonprofits only -
pHR_Gal4UAS_humanIL-10_T2A_PDL1_PGK_mCherry
Plasmid#85430Purpose[Edit] Lentiviral vector for Gal4 inducible human IL-10/PD-L1 with constitutive mCherry expressionDepositorInserthuman IL-10 T2A PD-L1
UseLentiviralExpressionMammalianAvailable SinceAug. 10, 2017AvailabilityAcademic Institutions and Nonprofits only