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172,829 results

Showing: 11911 - 11940 of 172829 results

  1. pComb3XTT

    Plasmid
    #63891
    Purpose
    Backbone of pComb3X containing TT Fab (light and heavy chains) against tetanus toxin, used as phage display/expression control and PCR template for human antibody constant regions (kappa and CH1)
    Depositor
    Carlos Barbas
    Article
    Andris-Widhopf et al J Immunol Methods. 2000 Aug 28;242(1-2):
    Insert
    TT Fab
    Use
    Phage display
    Tags
    6xHis, HA tag, and geneIII
    Expression
    Bacterial
    Promoter
    lacZ
    Available Since
    July 21, 2015
    Availability
    Academic Institutions and Nonprofits only
    63891-plasmid-map-sequence-id-450336

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  5. FRT_htr2c(c)-GFP

    Plasmid
    #79677
    Purpose
    serotonin receptor minigene with consensus splice sites that expresses the full length serotonin receptor with a C-terminal EGFP
    Depositor
    Stefan Stamm
    Article
    Zhang et al EMBO Mol Med. 2016 Aug 1;8(8):878-94. do
    Insert
    serotonin receptor 2C (HTR2C Human)
    Tags
    EGFP in Frame
    Expression
    Mammalian
    Mutation
    splice site of exon Vb in consensus
    Promoter
    CMV
    Available Since
    Aug. 30, 2016
    Availability
    Academic Institutions and Nonprofits only

  9. plex_307-ccdB-g418

    Plasmid
    #201141
    Purpose
    pLEX_307 plasmid with g418 marker
    Depositor
    Alejandro Chavez
    Type
    Empty backbone
    Expression
    Mammalian
    Promoter
    EF-1α
    Available Since
    June 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    201141-plasmid-map-sequence-id-395514

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  21. pET28a/Cas9-Cys

    Plasmid
    #53261
    Purpose
    Expresses N-terminal His tag fused human codon-optimized Cas9 nuclease having C-terminal Cysteine
    Depositor
    Hyongbum Kim
    Article
    Ramakrishna et al Genome Res. 2014 Apr 2.
    Insert
    Cas9-Cys
    Tags
    6X His tag, HA epitope, and NLS
    Expression
    Bacterial
    Mutation
    Additional Cysteine is added to the C-terminal re…
    Promoter
    T7 Promoter
    Available Since
    June 12, 2014
    Availability
    Academic Institutions and Nonprofits only
    53261-plasmid-map-sequence-id-261354

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  22. Tn7 integration plasmid

    Plasmid
    #154134
    Purpose
    Suicidal integration plasmid with the Tn7 arms, streptomycin and kanamycin resistance genes and the restriction site to clone the barcode-carrying cassette.
    Depositor
    Shimon Bershtein
    Article
    Jasinska et al Nat Ecol Evol. 2020 Feb 24. pii: 10.1038
    Inserts
    left and right end of Tn7 arm
    Spectinomycin resistant gene
    Expression
    Bacterial
    Promoter
    Spectinomycin resistance gene promoter and no
    Available Since
    July 24, 2020
    Availability
    Academic Institutions and Nonprofits only
    154134-plasmid-map-sequence-id-306811

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  25. pME-Cas9-T2A-GFP

    Plasmid
    #63155
    Purpose
    Middle Entry clone for Gateway containing a zebrafish codon-optimized Cas9 flanked by 2 NLS and followed by inframe GFP. Cas9 and GFP are separated by the sequence of a T2A self-cleaving peptide.
    Depositor
    Leonard Zon
    Article
    Ablain et al Dev Cell. 2015 Mar 4. pii: S1534-5807(15
    Insert
    Cas9-T2A-GFP
    Use
    CRISPR; Tol2 middle entry vector for gateway
    Available Since
    April 1, 2015
    Availability
    Academic Institutions and Nonprofits only
    63155-plasmid-map-sequence-id-220487

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Showing: 11911 - 11940 of 172829 results