2,223 results
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Plasmid#105648PurposeExpression of GFP in sensory neurons (retinal ganglion cells, trigeminal, Rohon Beard neurons and dorsal root ganglia) for up to 2 weeksDepositorInsertTol2-Isl2b:GFP:polyA (isl2b Zebrafish)
ExpressionMammalianAvailable SinceFeb. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
p5E-mitfa2.1
Plasmid#81234Purposezebrafish mitfa promoter entry vector for multisite Gateway cloningDepositorInsertmitfa promoter (mitfa Zebrafish)
UseMultisite gateway entry vectorPromotermitfa, 2.1kb including 5' UTRAvailable SinceSept. 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFRT-DestFLAGHA_RNAseH1
Plasmid#65783Purposeconstitutively expresses FLAG-HA-tagged RNAseH1 in mammalian cellsDepositorAvailable SinceJune 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pU6a:sgRNA(tyr)
Plasmid#64250Purposeexpresses sgRNA(tyr) under U6a promoterDepositorInsertU6a:sgRNA (tyr)
UseCRISPRAvailable SinceMay 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
Tg (mpx:mCherry-2A-Rac2D57N)
Plasmid#58934Purposeexpresses dominant negative rac2 tagged with mcherry in neutrophilsDepositorInsertmcherry-2a-Rac2D57N (rac2 Zebrafish)
Tagsmcherry2aMutationchanged Aspartate 57 to AsparaginePromotermpxAvailable SinceSept. 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
I-SceI pB 5UAS::ZfSyGCaMP2
Plasmid#26125DepositorAvailable SinceOct. 18, 2010AvailabilityAcademic Institutions and Nonprofits only -
pSB-CMV-MCS-Puro dr_cPLA2-mKate2
Plasmid#163844PurposeUsed as a driver for the readout of cPLA2 activation in live cells with an mKate2 tag.DepositorInsertcytosolic phospholipase a2 (pla2g4aa Zebrafish)
TagsmKate2ExpressionMammalianPromoterCMVAvailable SinceMarch 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKE4-I-SceI-fabp10a:Xpt-β-cat, cryaa:Venus
Plasmid#105127PurposeUsed along with I-SceI enzyme to generate transgenic zebrafish expressing hepatocyte-specific Xenopus activated beta-catenin and lens-specific Venus fluorescent proteinDepositorInsertsUseZebrafish transgenesisPromotercryaa (crystallin) and fabp10aAvailable SinceApril 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
14UAS PSD95:GFP 5UAS DSRedExpress
Plasmid#74315PurposeEncodes a PSD95:GFP fusion protein and DsRedExpress from separate UAS cassettes; for long-term two-photon time-lapse imaging of dendrite growth and synaptogenesis in zebrafishDepositorInsertszebrafish PSD95
DsRedExpress
TagsEGFPMutationY599H and I793S (please see depositor comment bel…Available SinceMay 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
ZE32 Prickle-1-GFP-pCS2+
Plasmid#17073DepositorAvailable SinceFeb. 1, 2008AvailabilityAcademic Institutions and Nonprofits only -
MiniCoopR mitfa:BRAF
Plasmid#118846PurposeExpresses human BRAF V600E mutant and zebrafish mitfa specifically in zebrafish melanocytesDepositorAvailable SinceDec. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUAS-Ncad-GFP
Plasmid#35986DepositorAvailable SinceApril 19, 2012AvailabilityAcademic Institutions and Nonprofits only -
slc1a3b:myrGFP-P2A-H2AmCherry
Plasmid#170208PurposeDrives myrGFP-P2A-H2AmCherry expression with the slc1a3b promoter in astrocytes. The myrGFP labels the membrane and mCherry labels the nucleusDepositorAvailable SinceJune 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
MiniCoopR mitfa:EGFP
Plasmid#118850PurposeExpresses EGFP and zebrafish mitfa specifically in zebrafish melanocytesDepositorInsertmitfa: EGFP (mitfa Zebrafish, Synthetic)
UseZebrafish expression, tol6Available SinceDec. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTol2p2A--3mnx1:mTagBFP-NS-MCS
Plasmid#78250PurposeCan be used to subclone in gene of interest into blue motor neuron expression vector.DepositorInsertsUseZebrafish motor neuron expressionPromoter-3mnx1Available SinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
5UAS zfSynaptophysin:GFP
Plasmid#74316PurposeA fusion of zebrafish synaptophysin to GFP under the control of 5UAS repeatsDepositorInsertsynaptophysin
TagsEGFPMutationQ12H and deletion of F13 (please see depositor co…Promoter5UASAvailable SinceMay 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
5UAS zfSynaptophysin:GFP-5UAS DsRedExpress
Plasmid#74317PurposeA fusion of zebrafish synaptophysin to GFP and DsRedExpress under the control of separate UAS cassettes; for dual-labeling of retinal ganglion cell (RGC) axon arbors in zebrafishDepositorInsertssynaptophysin
DsRedExpress
TagsEGFPPromoter5UASAvailable SinceMay 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pT7goldRNA
Plasmid#47930PurposeIn vitro transcription of golden gRNADepositorInsertgolden gRNA (slc24a5 Zebrafish)
UseCRISPRAvailable SinceAug. 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
M58 pPET28b-TEV-full-length zebrafish beta catenin
Plasmid#17199DepositorAvailable SinceFeb. 1, 2008AvailabilityAcademic Institutions and Nonprofits only -
pGTag-TagRFP-CAAX-SV40
Plasmid#117809PurposeDonor for precise CRISPR directed genomic integration using the GeneWeld methodDepositorInsertTagRFP
UseSynthetic BiologyTagsCAAXAvailable SinceFeb. 4, 2019AvailabilityAcademic Institutions and Nonprofits only