2,246 results
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Plasmid#65783Purposeconstitutively expresses FLAG-HA-tagged RNAseH1 in mammalian cellsDepositorAvailable sinceJune 18, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by
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pU6a:sgRNA(tyr)
Plasmid#64250Purposeexpresses sgRNA(tyr) under U6a promoterDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertU6a:sgRNA (tyr)
UseCRISPRAvailable sinceMay 7, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Tg (mpx:mCherry-2A-Rac2D57N)
Plasmid#58934Purposeexpresses dominant negative rac2 tagged with mcherry in neutrophilsDepositorInsertmcherry-2a-Rac2D57N (rac2 Zebrafish)
Tagsmcherry2aMutationchanged Aspartate 57 to AsparaginePromotermpxAvailable sinceSept. 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
I-SceI pB 5UAS::ZfSyGCaMP2
Plasmid#26125PurposeTransgenesis of zebrafish SyGCaMP2 (zebrafish synaptophysin fused to GCaMP2) with 5xUAS promoterDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSynaptophysin GCaMP2 (sypb Zebrafish)
UseZebrafish expressionTagsGCaMP2Available sinceOct. 18, 2010AvailabilityAcademic Institutions and Nonprofits only -
14UAS PSD95:GFP 5UAS DSRedExpress
Plasmid#74315PurposeEncodes a PSD95:GFP fusion protein and DsRedExpress from separate UAS cassettes; for long-term two-photon time-lapse imaging of dendrite growth and synaptogenesis in zebrafishDepositorInsertsTagsEGFPMutationY599H and I793S (please see depositor comment bel…Available sinceMay 19, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
ZE32 Prickle-1-GFP-pCS2+
Plasmid#17073DepositorAvailable sinceFeb. 1, 2008AvailabilityAcademic Institutions and Nonprofits only -
MiniCoopR mitfa:BRAF
Plasmid#118846PurposeExpresses human BRAF V600E mutant and zebrafish mitfa specifically in zebrafish melanocytesDepositorAvailable sinceDec. 3, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pUAS-Ncad-GFP
Plasmid#35986DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertNcad (cdh2 Zebrafish)
UseGal4 inducibleTagsEGFPPromoter14xUAS-E1bAvailable sinceApril 19, 2012AvailabilityAcademic Institutions and Nonprofits only -
slc1a3b:myrGFP-P2A-H2AmCherry
Plasmid#170208PurposeDrives myrGFP-P2A-H2AmCherry expression with the slc1a3b promoter in astrocytes. The myrGFP labels the membrane and mCherry labels the nucleusDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertslc1a3b (slc1a3b Zebrafish)
UseZebrafishTagsmyrGFP-P2A-H2AmCherryPromoterslc1a3bAvailable sinceJune 1, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
MiniCoopR mitfa:EGFP
Plasmid#118850PurposeExpresses EGFP and zebrafish mitfa specifically in zebrafish melanocytesDepositorInsertmitfa: EGFP (mitfa Synthetic, Zebrafish)
UseZebrafish expression, tol6Available sinceDec. 3, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMT-sox10-NLS-BirA-2A-mCherry_Ras
Plasmid#80063PurposeSox10 promoter driving HA-tagged biotin ligase (BirA) with NLS, a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequencesDepositorInsertBiotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein
UseZebrafish biotaggingTags3x HAExpressionBacterialPromoterSox10 promoterAvailable sinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTol2p2A--3mnx1:mTagBFP-NS-MCS
Plasmid#78250PurposeCan be used to subclone in gene of interest into blue motor neuron expression vector.DepositorPublicationHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsUseZebrafish motor neuron expressionPromoter-3mnx1Available sinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
5UAS zfSynaptophysin:GFP
Plasmid#74316PurposeA fusion of zebrafish synaptophysin to GFP under the control of 5UAS repeatsDepositorInsertsynaptophysin
TagsEGFPMutationQ12H and deletion of F13 (please see depositor co…Promoter5UASAvailable sinceMay 19, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
5UAS zfSynaptophysin:GFP-5UAS DsRedExpress
Plasmid#74317PurposeA fusion of zebrafish synaptophysin to GFP and DsRedExpress under the control of separate UAS cassettes; for dual-labeling of retinal ganglion cell (RGC) axon arbors in zebrafishDepositorInsertssynaptophysin
DsRedExpress
TagsEGFPPromoter5UASAvailable sinceMay 9, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pT7goldRNA
Plasmid#47930PurposeIn vitro transcription of golden gRNADepositorInsertgolden gRNA (slc24a5 Zebrafish)
UseCRISPRAvailable sinceAug. 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
M58 pPET28b-TEV-full-length zebrafish beta catenin
Plasmid#17199DepositorAvailable sinceFeb. 1, 2008AvailabilityAcademic Institutions and Nonprofits onlyCited by -
slc1a3b:myrGCaMP6s-P2A-H2AmCherry
Plasmid#170209PurposeDrives myrGCaMP6s-P2A-H2AmCherry expression with the slc1a3b promoter in astrocytes. The myrGCaMP6s calcium indicator expressed in the membrane and mCherry labels the nucleusDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertslc1a3b (slc1a3b Zebrafish)
UseZebrafishTagsmyrGCaMP6s-P2A-H2AmCherryPromoterslc1a3bAvailable sinceJune 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGTag-TagRFP-CAAX-SV40
Plasmid#117809PurposeDonor for precise CRISPR directed genomic integration using the GeneWeld methodDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertTagRFP
UseSynthetic BiologyTagsCAAXAvailable sinceFeb. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
p5E-mnx1
Plasmid#82402PurposeGateway-compatible zebrafish mnx1 promoterDepositorInsertmnx1 promoter
Available sinceNov. 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCS2vegf165
Plasmid#22417DepositorPublicationHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertvegf (vegfaa Zebrafish)
UseZebrafish expressionAvailable sinceDec. 10, 2009AvailabilityAcademic Institutions and Nonprofits onlyCited by