2,159 results

1. pTol2-HuC(elavl3)-CaMPARI2

   Plasmid

   #137185

   Purpose

   pan-neuronal expression of CaMPARI2 in zebrafish, used to generate the CaMPARI2 zebrafish line at ZIRC (ZL13801)

   Depositor

   Eric Schreiter

   Article

   Moeyaert et al Nat Commun. 2018 Oct 25;9(1):4440. doi:

   Insert

   HuC(elavl3)-CaMPARI2-FLAG-HA-myc-polyA
   

   Use

   Tol2 plasmid for zebrafish

   Tags

   FLAG, HA, myc

   Expression

   Mutation

   Promoter

   HuC(elavl3)

   Available since

   March 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGEM-NLS-Cre-2A-mCherry-FKF

   Plasmid

   #119868

   Purpose

   Donor cassette containing NLS-tagged Cre, a Thosea asigna virus peptide (2A), mCherry and SV40 polyA tail, followed by FRT site-flanked Kanamycin selection gene

   Depositor

   Tatjana Sauka-Spengler

   Article

   Weinberger et al Dev Cell. 2020 Mar 9;52(5):574-590.e6. d

   Insert

   NLS-Cre, 2A, mCherry and SV40 polyA, followed by FRT site-flanked Kanamycin selection gene
   

   Use

   Tags

   mCherry

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. p5'Entry-lck-7.4kb

   Plasmid

   #58891

   Purpose

   provides zebrafish lck promoter (7.4 kb) for GateWay construction of Tol2 transgenes

   Depositor

   Jeffrey Yoder

   Insert

   zebrafish lymphocyte-specific protein tyrosine kinase (lck) promoter, 7.4 kb
   

   Use

   Gateway 5' entry clone

   Tags

   Expression

   Mutation

   mutated single nucleotide (T-->C, 1874th nucle…

   Promoter

   lymphocyte-specific protein tyrosine kinase (lck)…

   Available since

   Aug. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pT2KXIG (Tol2)-tp63:ikk1:GFP

   Plasmid

   #105646

   Purpose

   Ikk1(alpha) expression in basal keratinocytes

   Depositor

   Sandra Rieger

   Article

   Lisse et al J Cell Sci. 2017 Mar 1;130(5):975-988. d

   Insert

   tp63:ikk1:GFP (chuk Zebrafish)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. p3E_he1a:eYFP

   Plasmid

   #113879

   Purpose

   p3E entry vector for Three Multisite Gateway

   Depositor

   Marnie Halpern

   Insert

   he1a (he1.1 Zebrafish)
   

   Use

   Zebrafish danio expression

   Tags

   eYFP

   Expression

   Mutation

   Promoter

   Available since

   Sept. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTol2-hsp70l:Cas9-t2A-GFP, 5xU6:sgRNA

   Plasmid

   #108871

   Purpose

   Expression of heat shock inducible Cas9-GFP and U6-driven 5 individual gRNAs for scGESTALT in zebrafish

   Depositor

   Alex Schier

   Article

   Raj et al Nat Biotechnol. 2018 Mar 28. pii: nbt.41

   Inserts

   5xU6:sgRNA
   Cas9-t2A-GFP
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   U6 and hsp70

   Available since

   April 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTol2p2A--3mnx1:mTagBFP-NS-MCS

   Plasmid

   #78250

   Purpose

   Can be used to subclone in gene of interest into blue motor neuron expression vector.

   Depositor

   Nicholas Cole

   Article

   Don et al Zebrafish. 2016 Sep 15.

   Inserts

   -3mnx1 (mnx1 Zebrafish)
   -mTagBFP-NS-MCS
   

   Use

   Zebrafish motor neuron expression

   Tags

   Expression

   Mutation

   Promoter

   -3mnx1

   Available since

   Oct. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. fabp10a:lox2272-loxp-nls-mTagBFP2-stop-lox2272-H2B-mGL-stop-loxp-mCherry-NTR; cryaa:mCherry

   Plasmid

   #230043

   Purpose

   Partial ablation and subsequent lineage tracing of regenerating hepatocytes in zebrafish.

   Depositor

   Sumeet Pal Singh

   Article

   Eski et al Nat Commun. 2025 Jun 6;16(1):5260. doi:

   Insert

   lox2272-loxp-nls-mTagBFP2-stop-lox2272-H2B-mGL-stop-loxp-mCherry-NTR
   

   Use

   Cre/Lox; Zebrafish expression

   Tags

   Expression

   Mutation

   Promoter

   fabp10a

   Available since

   Feb. 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pT2KXIG (Tol2)-tp63:ikk1KD:GFP

   Plasmid

   #105645

   Purpose

   Kinase-deleted Ikk1(alpha) expression in basal keratinocytes

   Depositor

   Sandra Rieger

   Article

   Lisse et al J Cell Sci. 2017 Mar 1;130(5):975-988. d

   Insert

   tp63:ikk1KD:GFP (chuk Zebrafish)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   p63 promoter + ikk1 (kinase domain deleted) + GFP

   Promoter

   Available since

   Feb. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pME-cas9-2A-mCherry

    Plasmid

    #107592

    Purpose

    An entry vector with cas9 -2A-mCherry

    Depositor

    Qing Deng

    Article

    Zhou et al Dis Model Mech. 2018 Mar 28;11(3). pii:

    Insert

    cas9-2A-mCherry
    

    Use

    CRISPR

    Tags

    2A-mCherry

    Expression

    Mutation

    Promoter

    Available since

    July 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCS2(+)-zGrad

    Plasmid

    #119716

    Purpose

    Plasmid to generate zGrad mRNA to inactivate proteins in zebrafish

    Depositor

    Holger Knaut

    Article

    Yamaguchi et al Elife. 2019 Feb 8;8. pii: 43125. doi: 10

    Insert

    zGrad
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCS2+cPLA2-mKate2

    Plasmid

    #162068

    Purpose

    Used to provide a steady-state readout of cPLA2 activation in live cells. Amino-terminally fused to mKate2 for red fluorescence.

    Depositor

    Philipp Niethammer

    Article

    Enyedi et al Nat Cell Biol. 2013 Sep;15(9):1123-30. d

    Insert

    cytosolic phospholipase a2 (pla2g4aa Zebrafish)
    

    Use

    Avian, xenopus, zebrafish

    Tags

    mKate2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSCAC-69

    Plasmid

    #31241

    Purpose

    Mitochondrial localization of EGFP, for real-time imaging of mitochondria in transgenic zebrafish

    Depositor

    Seok-Yong Choi

    Article

    Kim et al Biotechniques. 2008 Sep . 45(3):331-4.

    Insert

    mitochondrial localization enhanced green fluorescent protein
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pVC-Tol2-Pax3a_enh5-E1b:eGFP-Tol2

    Plasmid

    #119061

    Purpose

    Fluorescent vector with zebrafish pax3a enhancer 5; Tol2 version

    Depositor

    Tatjana Sauka-Spengler

    Article

    Chong-Morrison et al Biol Open. 2023 Jun 15;12(6):bio059995.

    Insert

    pax3a_enh5-E1b:eGFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    E1b minimal promoter

    Available since

    Feb. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. slc1a3b:myrGCaMP6s-P2A-H2AmCherry

    Plasmid

    #170209

    Purpose

    Drives myrGCaMP6s-P2A-H2AmCherry expression with the slc1a3b promoter in astrocytes. The myrGCaMP6s calcium indicator expressed in the membrane and mCherry labels the nucleus

    Depositor

    Kelly Monk

    Article

    Chen et al Nat Neurosci. 2020 Oct;23(10):1297-1306.

    Insert

    slc1a3b (slc1a3b Zebrafish)
    

    Use

    Zebrafish

    Tags

    myrGCaMP6s-P2A-H2AmCherry

    Expression

    Mutation

    Promoter

    slc1a3b

    Available since

    June 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSB-CMV-MCS-Puro cPLA2-EGFP

    Plasmid

    #162568

    Purpose

    Used as a measure of cPLA2 activation in live cells with an EGFP tag.

    Depositor

    Philipp Niethammer

    Article

    Enyedi et al Cell. 2016 May 19;165(5):1160-1170. doi:

    Insert

    cytosolic phospholipase a2 (pla2g4aa Zebrafish)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. 5UAS zfSynaptophysin:GFP-5UAS DsRedExpress

    Plasmid

    #74317

    Purpose

    A fusion of zebrafish synaptophysin to GFP and DsRedExpress under the control of separate UAS cassettes; for dual-labeling of retinal ganglion cell (RGC) axon arbors in zebrafish

    Depositor

    Stephen Smith, Martin Meyer

    Article

    Meyer et al J Neurosci. 2006 Mar 29;26(13):3604-14.

    Inserts

    synaptophysin
    DsRedExpress
    

    Use

    Tags

    EGFP

    Expression

    Mutation

    Promoter

    5UAS

    Available since

    May 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. p5E--3mnx1

    Plasmid

    #74632

    Purpose

    Can be used to drive expression specifically in zebrafish motor neurons

    Depositor

    Nicholas Cole

    Article

    Don et al Zebrafish. 2016 Sep 15.

    Insert

    motor neuron and pancreas homeobox 1 promoter (mnx1 Zebrafish)
    

    Use

    5' entry vector for multisite gateway three …

    Tags

    Expression

    Mutation

    Promoter

    -3mnx1

    Available since

    Oct. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. p5E-3x125bp-mnx1

    Plasmid

    #169043

    Purpose

    5' entry vector for Gateway cloning for expression in spinal motor neurons using 3 tandem repeats of the zebrafish mnx1 enhancer.

    Depositor

    Paola Arlotta

    Article

    Harris et al Dev Cell. 2020 Jun 8;53(5):577-588.e7. d

    Insert

    Mnx1 enhancer (3x) (mnx1 Zebrafish)
    

    Use

    Gateway entry cloning vector

    Tags

    Expression

    Mutation

    Promoter

    3xMnx1

    Available since

    Jan. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. p5E-dr-myl7-pro (JDW 1148)

    Plasmid

    #229836

    Purpose

    A gateway compatible 5' entry clone containing the mysoin light chain seven (myl7/cmlc2) promoter for cardiomyocyte specific expression.

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv 2024.07.13.603267

    Insert

    cmlc2/myl7
    

    Use

    Gateway entry clone

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge