2,162 results

1. pDrVSP-IRES2-EGFP

   Plasmid

   #80333

   Purpose

   Expresses Danio rerio VSP with separated EGFP in mammalian cell

   Depositor

   Yasushi Okamura

   Article

   Hossain et al J Biol Chem. 2008 Jun 27;283(26):18248-5

   Insert

   Daino rerio voltage-sensing phosphatase (tpte Zebrafish)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Sept. 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. hsp70l-loxP-mCherry-STOP-loxP-H2B-GFP_cryaa-cerulean

   Plasmid

   #24334

   Depositor

   Didier Stainier

   Article

   Hesselson et al Proc Natl Acad Sci U S A. 2009 Sep 1. 10

   Insert

   H2B
   

   Use

   Cre/Lox

   Tags

   GFP

   Expression

   Worm

   Mutation

   mCherry driven by an HSP70 promoterCerulean drive…

   Promoter

   Available Since

   April 27, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDestTol2pA2_ubi:EGFP

   Plasmid

   #27323

   Depositor

   Leonard Zon

   Article

   Mosimann et al Development. 2011 Jan . 138(1):169-77.

   Insert

   ubiquitin:EGFP reporter
   

   Use

   Tol2 transposon

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Feb. 3, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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4. pME-Cas9

   Plasmid

   #63154

   Purpose

   Middle Entry clone for Gateway containing a zebrafish codon-optimized Cas9 flanked by 2 NLS

   Depositor

   Leonard Zon

   Article

   Ablain et al Dev Cell. 2015 Mar 4. pii: S1534-5807(15

   Insert

   Cas9
   

   Use

   CRISPR; Tol2 middle entry vector for gateway

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   April 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGTag-gal4-VP16-B-actin

   Plasmid

   #117817

   Purpose

   Donor for precise CRISPR directed genomic integration using the GeneWeld method

   Depositor

   Jeffrey Essner

   Article

   Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

   Insert

   gal4/VP16
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   March 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. Tol2-elavl3-H2B-GCaMP6s

   Plasmid

   #59530

   Purpose

   Expressed nuclear-localized GCaMP6-slow, a calcium indicator, in neurons in zebrafish larva. The plasmid is in Tol2 transposon backbone

   Depositor

   Misha Ahrens

   Article

   Freeman et al Nat Methods. 2014 Jul 27. doi: 10.1038/n

   Inserts

   elavl3 promoter (elavl3 Zebrafish)
   H2B
   GCaMP6s
   

   Use

   Transposable element

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Sept. 12, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSB-CMV-MCS-Puro dr_cPLA2-mKate2

   Plasmid

   #163844

   Purpose

   Used as a driver for the readout of cPLA2 activation in live cells with an mKate2 tag.

   Depositor

   Philipp Niethammer

   Article

   Enyedi et al Cell. 2016 May 19;165(5):1160-1170. doi:

   Insert

   cytosolic phospholipase a2 (pla2g4aa Zebrafish)
   

   Use

   Tags

   mKate2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   March 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. p5E LyzC

   Plasmid

   #135200

   Purpose

   Neutrophil specific label

   Depositor

   David Tobin

   Article

   Oehlers et al Nature. 2015 Jan 29;517(7536):612-5. doi

   Insert

   Lyz promoter
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   LyzC

   Available Since

   Dec. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBS U6a gRNA

   Plasmid

   #158961

   Purpose

   gRNA backbone driven by zebrafish U6a promoter

   Depositor

   Michael Brand

   Article

   Hans et al Nat Commun. 2021 Feb 18;12(1):1125. doi:

   Inserts

   zf U6a promoter
   gRNA backbone
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Feb. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. 5UAS zfSynaptophysin:GFP-5UAS DsRedExpress

    Plasmid

    #74317

    Purpose

    A fusion of zebrafish synaptophysin to GFP and DsRedExpress under the control of separate UAS cassettes; for dual-labeling of retinal ganglion cell (RGC) axon arbors in zebrafish

    Depositor

    Stephen Smith, Martin Meyer

    Article

    Meyer et al J Neurosci. 2006 Mar 29;26(13):3604-14.

    Inserts

    synaptophysin
    DsRedExpress
    

    Use

    Tags

    EGFP

    Expression

    Mutation

    Promoter

    5UAS

    Available Since

    May 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pUFlip-floxed2A-mRFP-; gcry1:BFP -0

    Plasmid

    #173886

    Purpose

    Donor for generating conditional alleles in zebrafish using precise CRISPR directed genomic integration with the GeneWeld method

    Depositor

    Jeffrey Essner

    Article

    Liu et al Elife. 2022 Jun 17;11. pii: 71478. doi:

    Insert

    2A-mRFP; gcry1: BFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pKE12-ISceI-fabp10a:loxP-BFP-loxP-Xla.Ctnnb1

    Plasmid

    #198240

    Purpose

    For I-SceI-mediated transgenesis in zebrafish; fabp10a promoter drives expression of activated β-catenin preceded by a blue fluorescent protein (BFP)-STOP cassette flanked by loxP sites

    Depositor

    Didier Stainier

    Article

    Kalasekar et al Biol Open. 2019 Oct 17;8(10):bio047829.

    Inserts

    Zebrafish fabp10a promoter:floxedBFP, Xenopus pt-beta-catenin coding sequence, polyA (fabp10a Frog)
    Zebrafish cryaa promoter:mcherry fluorescent protein coding sequence (cryaa Zebrafish)
    

    Use

    Zebrafish transgenesis

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pPRISM-TagRFP-cmlc2-eGFP

    Plasmid

    #117770

    Purpose

    Donor for precise CRISPR directed genomic integration using the GeneWeld method

    Depositor

    Jeffrey Essner

    Article

    Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

    Insert

    TagRFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTol2 hsp70l loxP-Non-FP-loxP-nCas9n-GFP

    Plasmid

    #158963

    Purpose

    Cre-dependet Cas9-GFP expression

    Depositor

    Michael Brand

    Article

    Kesavan et al Development. 2020 Jun 8;147(11). pii: de

    Inserts

    hsp70l promoter
    floxed Non-FP cassette
    Cas9-GFP
    

    Use

    CRISPR and Cre/Lox

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. MiniCoopR mitfa:KIT K642E

    Plasmid

    #118849

    Purpose

    Expresses human KIT K642E mutant and zebrafish mitfa specifically in zebrafish melanocytes

    Depositor

    Leonard Zon

    Article

    Ablain et al Science. 2018 Nov 1. pii: science.aau650

    Insert

    KIT K642E (KIT Zebrafish, Human)
    

    Use

    Zebrafish expression, tol5

    Tags

    Expression

    Mutation

    KIT K642E

    Promoter

    Available Since

    Dec. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pUFlip-floxed2A-mRFP-; HE1A:BFP -2

    Plasmid

    #180009

    Purpose

    Donor for generating conditional alleles in zebrafish using precise CRISPR directed genomic integration with the GeneWeld method

    Depositor

    Jeffrey Essner

    Article

    Liu et al Elife. 2022 Jun 17;11. pii: 71478. doi:

    Insert

    2A-mRFP; HE1A: BFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pUFlip-floxed2A-mRFP-; HE1A:BFP -0

    Plasmid

    #180007

    Purpose

    Donor for generating conditional alleles in zebrafish using precise CRISPR directed genomic integration with the GeneWeld method

    Depositor

    Jeffrey Essner

    Article

    Liu et al Elife. 2022 Jun 17;11. pii: 71478. doi:

    Insert

    2A-mRFP; HE1A: BFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pENTR5'_ubi:loxP-EGFP-loxP

    Plasmid

    #27322

    Depositor

    Leonard Zon

    Article

    Mosimann et al Development. 2011 Jan . 138(1):169-77.

    Insert

    ubiquitin:loxP-EGFP-loxP
    

    Use

    Multisite gateway 5' entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 5, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAWO-SUMO-3C_14M_zTDP2

    Plasmid

    #200512

    Purpose

    Expression of zebrafish TDP2 recombinant protein

    Depositor

    Antony Oliver

    Insert

    TDP2 (tdp2b Zebrafish)
    

    Use

    Tags

    HIS-SUMO

    Expression

    Bacterial

    Mutation

    L136N, A139S, C239T, K240R, K309Q, K314L, T315G, …

    Promoter

    Available Since

    Aug. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. p5E-gfap

    Plasmid

    #75024

    Purpose

    Can be used to drive expression in zebrafish astrocytes.

    Depositor

    Nicholas Cole

    Article

    Don et al Zebrafish. 2016 Sep 15.

    Insert

    glial fibrillary acidic protein promoter (gfap Zebrafish)
    

    Use

    5' entry vector for multisite gateway three …

    Tags

    Expression

    Mutation

    Promoter

    gfap

    Available Since

    Oct. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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