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We narrowed to 7,986 results for: 104

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Showing: 151 - 200 of 7986 results

  5. pTrig

    Plasmid
    #104576
    Purpose
    Copy number inducible plasmid. Contains mini-F origin for maintenance and P1 lytic replication machinery under Lac promoter for copy increase.
    Depositor
    Harris Wang
    Article
    Sheth et al Science. 2017 Dec 15;358(6369):1457-1461
    Insert
    PLac-repL
    Use
    Synthetic Biology
    Available Since
    Feb. 22, 2018
    Availability
    Academic Institutions and Nonprofits only
    104576-plasmid-map-sequence-id-201435

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  8. pTP434

    Plasmid
    #104167
    Purpose
    Bacterial expression plasmid of anti-GFP nanobody with 3x Cysteines for maleimide labeling
    Depositor
    Dirk Görlich
    Article
    Pleiner et al Elife. 2015 Dec 3;4:e11349. doi: 10.7554
    Insert
    Anti-GFP nanobody Enhancer PDB 3K1K (3x Cysteine)
    Tags
    14x Histidine tag and NEDD8 from Brachypodium dis…
    Expression
    Bacterial
    Mutation
    3x Cysteines located at bps 493-495, 520-522, and…
    Available Since
    Nov. 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    104167-plasmid-map-sequence-id-200061

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  11. pRVL-3

    Plasmid
    #104581
    Purpose
    Contains mouse IgG2c CH1/mutated CH2/CH3 domains, for cloning of antibody VH domains using SapI restriction enzyme. Abolished Fc-mediated immune effector functions
    Depositor
    Daniel Christ
    Article
    Vazquez-Lombardi et al Methods Mol Biol. 2018;1827:313-334. doi
    Insert
    Mouse IgG2c CH1/CH2/CH3 (mutated, no effector functions)
    Expression
    Mammalian
    Mutation
    L234A/L235E/G237A and E318A/K320A/K322A
    Promoter
    CMV
    Available Since
    Jan. 9, 2018
    Availability
    Academic Institutions and Nonprofits only
    104581-plasmid-map-sequence-id-201392

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  17. pGP-AAV-syn-FLEX-jGCaMP7f-WPRE

    Plasmid
    #104492
    Purpose
    AAV-mediated expression of ultrasensitive protein calcium sensor under the Syn promoter, Cre mediated flip-excision switch
    Depositor
    Douglas Kim, GENIE Project
    Article
    Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi
    Has Service
    AAV PHP.eB, AAV Retrograde, AAV1, AAV8, and AAV9
    Insert
    jGCaMP7f
    Use
    AAV and Cre/Lox
    Tags
    T7 epitope, Xpress tag, 6xHis
    Expression
    Mammalian
    Promoter
    Synapsin
    Available Since
    April 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    104492-plasmid-map-sequence-id-381181

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  20. pGP-AAV-CAG-FLEX-jGCaMP7f-WPRE

    Plasmid
    #104496
    Purpose
    AAV-mediated expression of ultrasensitive protein calcium sensor under the CAG promoter, Cre mediated flip-excision switch
    Depositor
    Douglas Kim, GENIE Project
    Article
    Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi
    Has Service
    AAV PHP.eB, AAV Retrograde, and AAV1
    Insert
    jGCaMP7f
    Use
    AAV and Cre/Lox
    Tags
    T7 epitope, Xpress tag, 6xHis
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    Dec. 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    104496-plasmid-map-sequence-id-474734

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  23. BB3cH_pGAP_23*_pPFK300_Cas9

    Plasmid
    #104912
    Purpose
    hCas9 under control of pPFK300 for direct cloning of HH-sgRNA-HDV PCR products for episomal expression in P. pastoris and selection on Hyg
    Depositor
    Brigitte Gasser
    Article
    Gassler et al Methods Mol Biol. 2019;1923:211-225. doi
    Inserts
    HH - FS23 linker - HDV
    human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS)
    Use
    CRISPR
    Expression
    Yeast
    Available Since
    Jan. 29, 2019
    Availability
    Academic Institutions and Nonprofits only
    104912-plasmid-map-sequence-id-206066

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  30. p-dCas9-HDAC1-H141A-Hygro

    Plasmid
    #104417
    Purpose
    transient expression of dCas9-dHDAC1 fusion protein
    Depositor
    Stephan Beck
    Insert
    dHDAC1 (HDAC1 Human)
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    H141A
    Promoter
    CMV promoter
    Available Since
    April 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    104417-plasmid-map-sequence-id-204223

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  38. YET1000: CAG-human dLbCpf1(D832A)-NLS-3xHA-3xFLAG-DmrA(X4)

    Plasmid
    #104571
    Purpose
    Mammalian expression vector for catalytically inactive Cpf1 from Lachnospiraceae bacterium (dLbCpf1) fused to four DmrA domains
    Depositor
    Keith Joung
    Article
    Tak et al Nat Methods. 2017 Oct 30. doi: 10.1038/n
    Insert
    human codon optimized ‘dead’ Cpf1 fused to four DmrA domains
    Use
    CRISPR
    Tags
    NLS-3xHA-3xFLAG-4xDmrA
    Expression
    Mammalian
    Mutation
    D832A
    Promoter
    CAG
    Available Since
    Jan. 17, 2018
    Availability
    Academic Institutions and Nonprofits only
    104571-plasmid-map-sequence-id-201436

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  40. BB3cK_pGAP_23*_pLAT1_Cas9

    Plasmid
    #104908
    Purpose
    hCas9 under control of LAT1 for direct cloning of HH-sgRNA-HDV PCR products for episomal expression in P. pastoris and selection on G418
    Depositor
    Brigitte Gasser
    Article
    Gassler et al Methods Mol Biol. 2019;1923:211-225. doi
    Inserts
    HH - FS23 linker - HDV
    human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS)
    Use
    CRISPR
    Expression
    Yeast
    Available Since
    Oct. 1, 2019
    Availability
    Academic Institutions and Nonprofits only
    104908-plasmid-map-sequence-id-206067

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  45. LentiRCas9-CUG

    Plasmid
    #104183
    Purpose
    Lentiviral transfer vector that carries U6-driven sgRNA targeting CUG repeats using a modified scaffold (Chen et al. Cell 2013) and CMV-driven PIN-dCas9. Derived from LentiCRISPR v2 (Zhang lab)
    Depositor
    Eugene Yeo
    Article
    Batra et al Cell. 2017 Aug 24;170(5):899-912.e10. do
    Insert
    U6-CUGsgRNA, EFS-PIN-dCas9
    Use
    CRISPR and Lentiviral
    Available Since
    May 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    104183-plasmid-map-sequence-id-201743

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  47. pSBY3_ligD-HygR

    Plasmid
    #104625
    Purpose
    CRISPR system used to genome editing in Mycobacterium smegmatis, expresses Fn Cpf1 and crRNA targeting ligD and hygromysin resistance gene.
    Depositor
    Sheng Yang
    Article
    Sun et al Biotechnol J. 2018 Sep;13(9):e1700588. d
    Insert
    FnCpf1
    Use
    CRISPR
    Expression
    Bacterial
    Mutation
    FnCpf1 codon-optimized C.glutamicum
    Promoter
    hsp60
    Available Since
    June 25, 2018
    Availability
    Academic Institutions and Nonprofits only
    104625-plasmid-map-sequence-id-201573

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Showing: 151 - 200 of 7986 results